LOCUS       sequence-430023-       11624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo gene amplification (HamAmp) of nerolidol synthetic module at
            RPL25 locus (15 copy). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11624)
  TITLE     Vickers Lab plasmids
REFERENCE   2  (bases 1 to 11624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pINER3R
FEATURES             Location/Qualifiers
     source          1..11624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             743..1117
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
     primer_bind     complement(4325..4341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4325..4341)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4338..4360)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4349..4365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4373..4379,4380..4397,4398..4403))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4418..4439
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4556..4573)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4727..5315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4807..4826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5486..6277,6278..6346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6109..6128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6347..6451)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6519..6537
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6575..6597)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6697..6716
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6910..6932
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6924..6941
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6925..6941
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8987..9428
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    8987..9104
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     9399..9422
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 cattacttca tgagataaat taactcgagt gtactaatcc aaggaggttt acggaccaga
       61 ggaactttcc agattcagat cacagcaata taggactgga aaacatcagg tagccgcact
      121 caacttgtaa ctggcaacta ctttgcatta aactccaatt aaatgcggta gaatcttttc
      181 agaaaaggta ttcaacgtca attcggaaag cttccttccg gaatggctta agtaggttgc
      241 aatttctttt tctattagta gctaaaaatg ggtcacgtga tctatattcg aaaggggcgg
      301 ttgcctcagg aaggcaccgg cggtctttcg tccgtgcgga gatatctgcg ccgttcaggg
      361 gtccatgtgc cttggacgat attaaggcag aaggcagtat cggggcggat cactccgaac
      421 cgagattagt taagcccttc ccatctcaag atggggagca aatggcatta tactcctgct
      481 agaaagttaa ctgtgcacat attcttaaat tatacaatgt tctggagagc tattgtttaa
      541 aaaacaaaca tttcgcaggc taaaatgtgg agataggatt agttttgtag acatatataa
      601 acaatcagta attggattga aaatttggtg ttgtgaattg ctcttcatta tgcaccttat
      661 tcaattatca tcaagaatag caatagttaa gtaaacacaa gattaacata ataaaaaaaa
      721 taattctttc agtggatcca aaatggaaca cgttgctttc ggttctgaag atattgaaaa
      781 cactttggct aaaatggatg atggtcaatt agacggttta gctttcggtg ctattcaatt
      841 ggacggtgat ggtaacattt tgcaatacaa tgctgctgaa ggtgatatta ctggtagaga
      901 tccaaaacaa gttattggta agaacttctt taaggacgtt gctccaggta ctgactctcc
      961 agagttctat ggtaagttta aggaaggtgt tgcttctggt aacttaaaca ctatgtttga
     1021 atggatgatc ccaacttcca gaggtccaac taaggttaaa gttcacatga aaaaggcttt
     1081 atctggtgac tcttactggg ttttcgttaa aagagttggt gctactaact tctctttgtt
     1141 gaagttggct ggtgacgttg aacttaaccc aggtccagct accgcagcag gtcctatcgc
     1201 aactaacaac tccccacaaa actccaacgc ttacagaact ccaatcgctc cttccgtacc
     1261 aattactcat aaatggtcta tagctgaaga tttgacatgt atttccaatc ctagtaagca
     1321 caataaccct caaactggtt acagatcatt ttctgacgaa ttatacgtta agtacgaaga
     1381 aaagttggaa gatgttagaa aagcattaag agaagttgaa gaaaaccctt tggaaggttt
     1441 agttatgata gacgctttgc aaagattggg tatcgattac catttcagag gtgaaattgg
     1501 tgcattcttg caaaagcaac aaatcatatc ttcaactcca gatggttacc ctgaacatgg
     1561 tttgtacgaa gtttcaacat tgtttagatt cttaagacaa gaaggtcaca atgttaccgc
     1621 tgacgtcttt aataacttca aggataagga aggtagattc agatcagaat tgtcaacaga
     1681 tattagaggt ttgatgtcct tatacgaagc aagtcaattg agaatagaag gtgaagacat
     1741 cttagatcaa gctgctgatt tctccagtca attgttaggt agatggacaa aagatcctaa
     1801 tcatcacgaa gccagattgg tttctaacac tttaacacat ccataccaca agtcattggc
     1861 tacctttatg ggtcaaaaat tgtcctacat gaactgcaag ggtccaaact gggacggtgt
     1921 cgataatttg caagaattag ctaagatgga tttgactatc gtacaaagta tccatcaaaa
     1981 ggaagtattc caagtttctc aatggtggaa ggatacaggt ttagccaatg aattgaaatt
     2041 ggctagaaac caaccattga agtggtatat gtggcctatg gccgctttaa ccgatccaag
     2101 attcagtgaa gaaagagttg aattaactaa gcctatttct tttatataca tcatagatga
     2161 catcttcgac gtctatggta ccattgaaga attgaccttg tttactgatg cagttaatag
     2221 atgggaattg tctgccgtcg aacaattacc agactacatg aaagtatgtt tcaaggcttt
     2281 gtacgatgtt accaacgaaa tcgcatacaa aatctataaa aagcatggtc aaaaccctat
     2341 tgattccttg caaaagactt gggctagttt gtgcaatgca tttttagttg aagcaaagtg
     2401 gttcgcctct ggtcacttgc caaatgcaga agaatactta aagaacggta tcatctcttc
     2461 aggtgttcat gttgtcttgg cccacatgtt tttcttgtta ggtgacggta ttacacaaga
     2521 atcagttgat ttggtagatg actatccagg tatttccaca agtatcgcaa ccattttgag
     2581 attatctgat gacttgggtt cagccaaaga tgaagaccaa gatggttatg atggttctta
     2641 catcgaatgt tacatgaagg aacataaggg ttccagtgtc gattcagcca gagaagaagt
     2701 aataagaatg atctccgaag catggaaatg tttgaataag gaatgcttat caccaaaccc
     2761 tttttctgaa tcattcagaa taggttcctt gaatatggct agaatgatcc ctatgatgta
     2821 ctcttacgat gacaaccata acttgccaat tttagaagaa cacatgaagg caatgatcta
     2881 taacacatct ttataatagt gaccatggcg gattgagagc aaatcgttaa gttcaggtca
     2941 agtaaaaatt gatttcgaaa actaatttct cttatacaat cctttgattg gaccgtcatc
     3001 ctttcgaata taagattttg ttaagaatat tttagacaga gatctacttt atatttaata
     3061 tctagatatt acataatttc ctctctaata aaatatcatt aataaaataa aaatgaagcg
     3121 atttgatttt gtgttgtcaa cttagtttgc cgctatgcct cttgggtaat gctattattg
     3181 aatcgaaggg ctttattata ttacccttta gcttattctg aggtttctgt ggcgtcccgg
     3241 gaatctcggt cgtaatgatt tgcatgcgaa attcaaaact ctccagacaa agcctgccat
     3301 ttggccaagc aagcttttga cgacgctatt gctgagttgg acactctgtc tgaagaatca
     3361 tacaaagata gcacacttat catgcaactg ctaagggaca atttaacctt atggacttca
     3421 gacatgtccg agtccggtca agctgaagac caacaacaac aacaacaaca tcagcaacag
     3481 cagccacctg ctgccgccga aggtgaagca ccaaagtaag tattccgata aatctaaaga
     3541 aaaattacta aaaaaaagaa aaaaaaaaga acgggggtat aataatttgt agttcattat
     3601 tgcaattata tatctatatc tatatatgta tataacatta acatgtgcac gtacacacgt
     3661 aatcgcgcgt gtacatgtct atatgtgtta cttgaactat actgttttga cgtgtatgtt
     3721 tgtttatctc tcttctgatt cctccacccc ttccttactc aaccgggtaa atgtcgcatc
     3781 atgactcccg acaataatcc cctctggtat agcgagaagc aacttcagct tcttaacggc
     3841 aagaactttt ttatgtttgt cgcacctgta gcttcacaaa agttggatac agcaataaga
     3901 aaggaaacca catttgtgcc aatggctcca tctggtatgt gaactgcaat attaatagca
     3961 cgagaaaatt gagaggaaga tagatgggaa ctagtagagt tgatattgat gagatacgaa
     4021 aaccacacgt aaataaacta tccgacgaca agaatagtgc attaaggact tgattaagat
     4081 atggtgagca acgtaattat cgggctcaac agtttattag caatcgtttt gatagaagcg
     4141 ttgatgctgt ggaagttgtt ctttttacta ccaacagcca ttaacaaatc cattagagtg
     4201 ttcgttcgtt tttctgctca gattcaagaa aattattcca tcctcattat acttttttct
     4261 tctatttcgt gctccacgtc gaggtatcaa ggaacatagt ttaaacaagc ttggcgtaat
     4321 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     4381 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     4441 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4501 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4561 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4621 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4681 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4741 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4801 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4861 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4921 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4981 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5041 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5101 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5161 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5221 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5281 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5341 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5401 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5461 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5521 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5581 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5641 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5701 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5761 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5821 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5881 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5941 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6001 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6061 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6121 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6181 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6241 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6301 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6361 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6421 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     6481 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     6541 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     6601 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     6661 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     6721 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6781 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6841 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     6901 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcagt ttaaacatgt
     6961 actaatcagt ctaacacccc ggaggaggct tatcattaca tattcttgtc tcggcgttct
     7021 ttaagtttct tatcttgtat gcccgatata gcaaccttgt tggtaccaat ctaacggttt
     7081 ccgtactttg caatgaagag atgaggaggc atgggtcact tatttaatat gtacgggtgt
     7141 ttacatggag ttgctttctt tttttgtctc agcagtcatt gtgcgccaaa aaaagagaaa
     7201 accgtgagcc gaagtccacg ctctggagtt aggctctccc attacggaga gaagcatttc
     7261 ctcagcctgg gagccccgtt ggaacagtca ggctaaactg ggccttccta cccactgctt
     7321 gctgtttctc actggacgca caaggggatt ttctttctac cttcggcttg cctcactgcg
     7381 ttggggcttc ccaatgcaac ttcgttcgta tgcatacaat cttttagata ttatctttta
     7441 aaattatttt aaaacaattt taaatgtatc tcatatgctt ttcttctgct gttgaaaagg
     7501 ctaaacaaag aagatcaata agataaagcg gccgcaaaat gtctaagaat atcgttgtcc
     7561 taccgggtga tcacgtcggt aaagaagtta ctgacgaagc tattaaggtc ttgaatgcca
     7621 ttgctgaagt ccgtccagaa attaagttca atttccaaca tcacttgatc gggggtgctg
     7681 ccatcgatgc cactggcact cctttaccag atgaagctct agaagcctct aagaaagccg
     7741 atgctgtctt actaggtgct gttggtggtc caaaatgggg tacgggcgca gttagaccag
     7801 aacaaggtct attgaagatc agaaaggaat tgggtctata cgccaacttg agaccatgta
     7861 actttgcttc tgattcttta ctagatcttt ctcctttgaa gcctgaatat gcaaagggta
     7921 ccgatttcgt cgtcgttaga gaattggttg gtggtatcta ctttggtgaa agaaaagaag
     7981 atgaaggtga cggagttgct tgggactctg agaaatacag tgttcctgaa gttcaaagaa
     8041 ttacaagaat ggctgctttc ttggcattgc aacaaaaccc accattacca atctggtctc
     8101 ttgacaaggc taacgtgctt gcctcttcca gattgtggag aaagactgtt gaagaaacca
     8161 tcaagactga gttcccacaa ttaactgttc agcaccaatt gatcgactct gctgctatga
     8221 ttttggttaa atcaccaact aagctaaacg gtgttgttat taccaacaac atgtttggtg
     8281 atattatctc cgatgaagcc tctgttattc caggttcttt gggtttatta ccttctgcat
     8341 ctctagcttc cctacctgac actaacaagg cattcggttt gtacgaacca tgtcatggtt
     8401 ctgccccaga tttaccagca aacaaggtta acccaattgc taccatctta tctgcagcta
     8461 tgatgttgaa gttatccttg gatttggttg aagaaggtag ggctcttgaa gaagctgtta
     8521 gaaatgtctt ggatgcaggt gtcagaaccg gtgaccttgg tggttctaac tctaccactg
     8581 aggttggcga tgctatcgcc aaggctgtca aggaaatctt ggcttaaaga gtcttttgta
     8641 acgaccccgt ctccaccaac ttggtatgct tgaaatctca aggccattac acattcagtt
     8701 atgtgaacga aaggtcttta tttaacgtag cataaactaa ataatacagg ttccggttag
     8761 cctgcgctag cttatattga attttcaaaa attcttactt tttttttgga tggacgcaaa
     8821 gaagtttaat aatcatatta catggcaata ccaccatata catatccata tctaatctta
     8881 cttatatgtt gtggaaatgt aaagagcccc attatcttag cctaaaaaaa ccttctcttt
     8941 ggaactttca gtaatacgct taactgctca ttgctatatt gaagtacgga ttagaagccg
     9001 ccgagcgggc gacagccctc cgacggaaga ctctcctccg tgcgtcctgg tcttcaccgg
     9061 tcgcgttcct gaaacgcaga tgtgcctcgc gccgcactgc tccgaacaat aaagattcta
     9121 caatactagc ttttatggtt atgaagagga aaaattggca gtaacctggc cccacaaacc
     9181 ttcaaatcaa cgaatcaaat taacaaccat aggataataa tgcgattagt tttttagcct
     9241 tatttctggg gtaattaatc agcgaagcga tgatttttga tctattaaca gatatataaa
     9301 tgcaaaagct gcataaccac tttaactaat actttcaaca ttttcggttt gtattacttc
     9361 ttattcaaat gtcataaaag tatcaacaaa aaattgttaa tatacctcta tactttaacg
     9421 tcaaggagaa aaaactatag ctagcaaaat ggcttcagaa aaagaaatta ggagagagag
     9481 attcttgaac gttttcccta aattagtaga ggaattgaac gcatcgcttt tggcttacgg
     9541 tatgcctaag gaagcatgtg actggtatgc ccactcattg aactacaaca ctccaggcgg
     9601 taagttaaat agaggtttgt ccgttgtgga cacgtatgct attctctcca acaagaccgt
     9661 tgaacaattg gggcaagaag aatacgaaaa ggttgctatt ctaggttggt gcattgagtt
     9721 gttgcaggct tacttcttgg tcgccgatga tatgatggac aagtccatta ccagaagagg
     9781 ccaaccatgt tggtacaagg ttcctgaagt tggggaaatt gccatcaatg acgcattcat
     9841 gttagaggct gctatctaca agcttttgaa atctcacttc agaaacgaaa aatactacat
     9901 agatatcacc gaattgttcc atgaagtcac cttccaaacc gaattgggcc aattgatgga
     9961 cttaatcact gcacctgaag acaaagtcga cttgagtaag ttctccctaa agaagcactc
    10021 cttcatagtt actttcaaga ctgcttacta ttctttctac ttgcctgtcg cattggctat
    10081 gtacgttgcc ggtatcacag atgaaaagga tttgaaacaa gccagagatg tcttgattcc
    10141 attgggtgaa tatttccaaa ttcaagatga ctacttagac tgcttcggta ccccagaaca
    10201 gatcggtaag atcggtacag atatccaaga taacaaatgt tcttgggtaa tcaacaaggc
    10261 attagaactt gcttccgcag aacaaagaaa gactttagac gaaaattacg gtaagaagga
    10321 ctcagtcgca gaagccaaat gcaaaaagat tttcaatgac ttgaaaatcg accagttata
    10381 ccacgaatat gaagagtctg ttgccaagga tttgaaggcc aagatctccc aagtcgacga
    10441 gtctcgtggc ttcaaagccg acgtcttaac tgcgtttttg aacaaagttt acaagagaag
    10501 caaataatag tgacctgcag gaagttttgt tagaaaataa atcatttttt aattgagcat
    10561 tcttattcct attttattta aatagtttta tgtattgtta gctacataca acagtttaaa
    10621 tcaaattttc tttttcccaa gtccaaaatg gaggtttatt ttgatgaccc gcatgcgatt
    10681 atgttttgaa agtataagac tacatacatg tacatatatt taaacatgta aacccgtcca
    10741 ttatattgct tactttcttc ttttttgccg ttttgacttg gacctctggt ttgctatttc
    10801 cttacaatct ttgctacaat gggcccctag ctctaattgg tttaattaat aaatttaata
    10861 ttatttttaa atttttcttt aaatatacaa taaatctttc ataacatgtt aaattcatga
    10921 ttaagcgtaa ataaagtgta gtggcagagt gcacggggtt tcctgtgcct tacaaagtag
    10981 gtaccaattt gcgtattgca gcgagggttc cggttactat ttataattac gtgttagtgt
    11041 actgtgattt tattgaggct ataacaagaa aaggatctgt gaaggttttg agagaatgag
    11101 gcgaaaacac agggttattt tcatcagcaa cgccgctgtt tcagcggcac cctgcccttt
    11161 tcttttcgca agatgagagt aaagagttgt acatcaggta agaatgttat tatttaaatt
    11221 cgaagtgata aattcttttc atgatgaatc actcgcttat atggggtaga atatatatat
    11281 atgtgtgtgt gtgtgtgtgt ttgtgtatgt agggatggtg cgcgtttgtt gtgtgacatt
    11341 tgctactcat tcttttcctt ttcctacgac tggcttaacg ggaatattat caatttgctg
    11401 cattcttatg cttcggtccg atgctcaagc ttaacttctt cgtgaggaag gaaagtgttg
    11461 tctcctactt ttttcaaatt ttcgaattgt atttatattt atttagtact tcttgagttt
    11521 acatatcctt cgtaaaaatg caacttttgt cgaaaaacac ttccaaaaaa aaataataat
    11581 gaatttatga agcatactaa cgagcgagca catcgctgag gtat
//
