LOCUS       sequence-430095-        8115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MBP-tagged full length Efg1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Wang SH, Zheng T, Fawzi NL
  TITLE     Structure and interactions of prion-like domains in transcription 
            factor Efg1 phase separation.
  JOURNAL   Biophys J. 2024 Jun 4;123(11):1481-1493. doi: 
            10.1016/j.bpj.2024.01.030. Epub 2024 Feb 1.
  PUBMED    38297837
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2024.01.030"; journalName: "Biophys J"; date: 
            "2024-06-4- 4"; volume: "123"; issue: "11"; pages: "1481-1493"
COMMENT     Sequence Label: His-MBP-FL-Efg1
FEATURES             Location/Qualifiers
     source          1..8115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      511..520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     628..651
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     protein_bind    673..697
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             706..726
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2401..2418
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2471..2489)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2485..2532
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2569..3024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2656..2675)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2866..2887
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3116..3931)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3116..3931)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3839..3858
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3923..3942
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4053..4641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4542..4561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4795..4812
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4827..4966
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4892..4911)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5052..5074
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5068..5259)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6068..7150)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7112..7131
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7151..7228)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7434..7453
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     7537..7556
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7537..7555
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7556..7580
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7595..7617
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7642..7659
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(7672..8115,1..654)
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
ORIGIN
        1 ttcaacctgc aagaaccgta cttcacctgg ccgctgattg ctgctgacgg gggttatgcg
       61 ttcaagtatg aaaacggcaa gtacgacatt aaagacgtgg gcgtggataa cgctggcgcg
      121 aaagcgggtc tgaccttcct ggttgacctg attaaaaaca aacacatgaa tgcagacacc
      181 gattactcca tcgcagaagc tgcctttaat aaaggcgaaa cagcgatgac catcaacggc
      241 ccgtgggcat ggtccaacat cgacaccagc aaagtgaatt atggtgtaac ggtactgccg
      301 accttcaagg gtcaaccatc caaaccgttc gttggcgtgc tgagcgcagg tattaacgcc
      361 gccagtccga acaaagagct ggcaaaagag ttcctcgaaa actatctgct gactgatgaa
      421 ggtctggaag cggttaataa agacaaaccg ctgggtgccg tagcgctgaa gtcttacgag
      481 gaagagttgg cgaaagatcc acgtattgcc gccaccatgg aaaacgccca gaaaggtgaa
      541 atcatgccga acatcccgca gatgtccgct ttctggtatg ccgtgcgtac tgcggtgatc
      601 aacgccgcca gcggtcgtca gactgtcgat gaagccctga aagacgcgca gactaattcg
      661 ggatctgata tcacaagttt gtacaaaaaa gctgaaggcg gtaccgagaa cctgtacttc
      721 cagggtcata tgagcaccta tagcattccg tattataatc agatgaacgg caactacaat
      781 aatggtatgc cgcagcagac caccgcagca aatcagcagg catttcctca gcagcagcaa
      841 ccgaccacca ccggtaatgc aagccagcaa cagcaacagg cagccgcaac cgcagcagcg
      901 gcagcagttc agcagccgta taactatatg ttttatcagc agcaaggtca gcctggtcag
      961 cagaccggtc agaccgcagg tcagcagcaa caacagcaac agcagcagca gcaatatgat
     1021 tataacacct ataaccgcta tcagtatccg gcagcaacca gccagggtaa ttactatcag
     1081 cagacaattc cgaatcagct gagccagccg cagcctcagc attataacgg tagcaatcgt
     1141 aattatacca gcgcaccgag tggtgcaccg attccgagca atagcaccag cggtccgagc
     1201 cagcagcctc cgctgcctgg acagcaggcc gttccgattc cgcctcatgt tagcaccatg
     1261 cagcagccga caccggttca ggataccctg aatgccagca gcaccagtac cgttggtcag
     1321 tttcagcctc cgggtattcg tccgcgtgtt accaccacca tgtgggaaga tgaaaaaacc
     1381 ctgtgttatc aggtggatgc caataatgtt agcgttgttc gtcgtgcaga taacaacatg
     1441 attaatggca ccaaactgct gaatgttgca cagatgaccc gtggtcgtcg tgatggtatt
     1501 ctgaaaagcg aaaaagttcg tcacgtggtg aaaattggta gcatgcatct gaaaggtgtt
     1561 tggattccgt ttgaacgtgc actggcaatg gcacagcgtg agcagattgt tgatatgctg
     1621 tatccgctgt ttgtgcgtga tattaaacgt gttattcaga ccggtgttac cccgaatgcg
     1681 gcagcggcta ctgcagctgc cgcagccacc gcaaccagcg caagtgcacc tcctccgcct
     1741 ccgccaccgg ttgcagcagc taccacaaca gcggcaaccg ccattagcaa aagcagctca
     1801 ggtaatggta atagcattag cgcgaccagc ggtggtagca atgttagtgg tgcaagcggt
     1861 gcaggtagca ccaccagtcc ggttaatacc aaagcagcga ccgcagccgg tattccgcag
     1921 ggcaactatt atcagaccta taatcagcaa cagtacccgc agcagtatgg tcagtataat
     1981 gcaccgggta aaaatcagaa tacaccggca tcacagcctg gtagtaccac caatgatcag
     2041 tatctgcagc agcagcaaca gcaacaacaa caaatgtatg gttatcagag caactactat
     2101 caaggtggtg cagccaatag cagctattat ccgaattatt accaacaaca gcagccgaat
     2161 tatgcaagca gctatccgta tcaacagcag caacaaaaac agcagcaaca acaaccgaat
     2221 cagcagcaac agagtgatca gcagcagacc tcaaccccga gcggtggtgc cggtacacgt
     2281 agcgttcatc agagtccgca ggttcagagc ctgacccagg gtagcgtgca tccgagccca
     2341 cagcagcatc aggcaaatca gagcgcaagc accgttgcaa aagaagagaa ataactcgag
     2401 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
     2461 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     2521 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
     2581 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     2641 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     2701 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     2761 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     2821 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     2881 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     2941 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     3001 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3061 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga
     3121 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     3181 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     3241 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     3301 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     3361 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     3421 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     3481 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     3541 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     3601 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     3661 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     3721 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     3781 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     3841 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
     3901 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     3961 cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4021 tcagaccccg tagaaaagat caaaggatct tcttgatatc ctttttttct gcgcgtaatc
     4081 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4141 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     4201 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4261 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4321 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4381 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4441 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4501 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4561 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4621 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4681 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4741 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4801 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     4861 ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     4921 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     4981 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     5041 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     5101 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     5161 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     5221 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     5281 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     5341 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     5401 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     5461 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     5521 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     5581 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     5641 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     5701 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
     5761 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
     5821 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
     5881 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
     5941 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
     6001 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     6061 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     6121 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     6181 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     6241 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     6301 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     6361 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     6421 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     6481 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     6541 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     6601 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     6661 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     6721 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     6781 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     6841 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     6901 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     6961 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     7021 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     7081 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     7141 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     7201 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     7261 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     7321 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     7381 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     7441 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     7501 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     7561 tgtgagcgga taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat
     7621 accatgggat ctgataaaat tcatcatcat catcatcaca gcagcggtac caaaatcgaa
     7681 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     7741 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     7801 gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca
     7861 cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     7921 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     7981 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     8041 aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     8101 aagagcgcgc tgatg
//
