LOCUS       sequence-430118-        7119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..7119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..260
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             273..541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     591..650
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             651..677
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3564..3587
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4302..4423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4313..4332)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4367..4386
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    4527..4921
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(4935..5309)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        5902..6205
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(6285..6873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6365..6384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    6981..7029
                     /note="synthetic polyadenylation signal"
     misc_feature    join(7040..7119,1..12)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7080..7099
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gaacatttct ctatcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
       61 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
      121 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      181 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      241 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
      301 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
      361 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
      421 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
      481 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
      541 ccaagctgtg accggcgcct acctgagatc accggcgaag gagggccacc atgtacagga
      601 tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg tacccatacg
      661 atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt tggattaacg
      721 gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa gataccggca
      781 ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag gtggcggcga
      841 ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc tatgcgcaga
      901 gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg tatccgttta
      961 cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg gtggaagcgc
     1021 tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg gaagaaattc
     1081 cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt aacctgcagg
     1141 aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt aaatatgaaa
     1201 acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa gcgggcctga
     1261 cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat tatagcattg
     1321 cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg tgggcgtgga
     1381 gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc tttaaaggcc
     1441 agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg agcccgaaca
     1501 aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc ctggaagcgg
     1561 tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa gaactggcga
     1621 aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt atgccgaaca
     1681 ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac gcggcgagcg
     1741 gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac ctttacttcc
     1801 aagaactgtc tgcctttcct cgcgaggtcg gtggcgctgg agcgggactc caccctgttg
     1861 tgtatccctg tacagccttg ctgttgctgt gcttgttcgc cactatcatt acttacattc
     1921 tgaatcactc ctcaattcgg gtatcccgca agggatggca catgctcctg aacctctgtt
     1981 tccacatcgc catgacaagc gccgtcttcg ccggtggcat caccctgacc aattatcaga
     2041 tggtgtgcca ggccgtaggt atcaccctgc actattcctc tctctccacc ctcctgtgga
     2101 tgggcgtaaa ggcccgtgtg cttcacaagg aactgacgtg gagggctcct ccaccgcagg
     2161 agggagatcc cgccctgcct acaccaagtc ccatgctgcg cttctacctg atcgccggcg
     2221 gtatcccatt gatcatttgc ggcatcacgg ctgcggtcaa tatccacaat tacagagatc
     2281 atagtcccta ttgctggctc gtgtggcgtc cttcactggg agctttctac attcccgttg
     2341 ctctcatcct tctgattact tggatttact tcctgtgtgc cgggttgcgc ctccgtgggc
     2401 ctctcgcgca gaatcctaag gccgggaact ccagggcctc cctggaggcc ggagaggaac
     2461 tgcgtggctc cacacgtctg agaggaagcg gcccactgtt gtctgattcc ggctcccttc
     2521 tggccactgg atctgctcgt gtgggaactc ccggtccgcc agaagatggt gactccttgt
     2581 acagtcccgg cgtccagctg ggagccttgg tcacaaccca ttttctgtat ctggctatgt
     2641 gggcctgtgg tgccctggcg gttagccagc gttggctgcc tcgcgttgtg tgcagctgtc
     2701 tctacggagt cgcagcctcc gctttgggcc tcttcgtttt tactcatcac tgtgctagac
     2761 ggcgcgacgt gagagcatca tggcgtgcct gttgccctcc ggcatcacca gccgctccgc
     2821 acgcaccacc tcgcgctctg ccggcagctg ccgaagatgg ttctcctgtc tttggcgagg
     2881 gcccgcccag cctcaagtct agccctagtg gaagctccgg ccacccgctg gcactgggac
     2941 cgtgcaagtt gaccaacctg cagttggctc agagccaagt gtgcgaagcc ggtgctgcgg
     3001 ctggcggtga gggcgaacca gaacccgccg gtacaagagg caatctggcc cataggcacc
     3061 cgaacaatgt tcatcacggt aggcgtgctc ataagtctcg tgccaaaggg caccgtgcgg
     3121 gtgaagcctg tggtaagaac cgcctcaaag ctttgcgcgg tggcgcggcc ggtgctctgg
     3181 agcttctgag cagtgaatct ggctctctgc acaacagtcc cactgactct tatctcgggt
     3241 cctcacgcaa ctcaccagga gccggtctcc aactggaggg cgaaccaatg ctgactccat
     3301 ccgaaggtag cgacacctca gcggcaccgt tgtccgaagc cggaagggca gggcaacgtc
     3361 gcagcgcctc acgtgacagc ctcaagggcg gaggcgccct ggaaaaggaa agtcataggc
     3421 gcagctaccc gctgaacgct gccagcctga atggtgctcc caagggaggt aagtatgatg
     3481 acgtgaccct tatgggcgcc gaggttgcca gtggcggttg catgaagacc ggtctgtgga
     3541 aatcagagac taccgtcggt tctgactaca aagacgatga cgacaagtga agatctgaca
     3601 aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc
     3661 tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg
     3721 tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg
     3781 tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg
     3841 tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca
     3901 aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc
     3961 agccccgaga accacaggtg tacaccctgc ccccatcccg ggaggagatg accaagaacc
     4021 aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg
     4081 agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg
     4141 gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg
     4201 tcttctcatg ctccgtgatg cacgaggctc tgcacaacca ctacacgcag aagagcctct
     4261 ccctgtctcc gggtaaatga gtgctagctg gccagacatg ataagataca ttgatgagtt
     4321 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     4381 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     4441 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     4501 ctacaaatgt ggtatggaat taattctaaa atacagcata gcaaaacttt aacctccaaa
     4561 tcaagcctct acttgaatcc ttttctgagg gatgaataag gcataggcat caggggctgt
     4621 tgccaatgtg cattagctgt ttgcagcctc accttctttc atggagttta agatatagtg
     4681 tattttccca aggtttgaac tagctcttca tttctttatg ttttaaatgc actgacctcc
     4741 cacattccct ttttagtaaa atattcagaa ataatttaaa tacatcattg caatgaaaat
     4801 aaatgttttt tattaggcag aatccagatg ctcaaggccc ttcataatat cccccagttt
     4861 agtagttgga cttagggaac aaaggaacct ttaatagaaa ttggacagca agaaagcgag
     4921 cttctagctt atcctcagtc ctgctcctct gccacaaagt gcacgcagtt gccggccggg
     4981 tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat ggccggcccg
     5041 gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag ctcgtccagg
     5101 ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg gaccgcgctg
     5161 atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg
     5221 gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc
     5281 accggaacgg cactggtcaa cttggccatg atggctcctc ctgtcaggag aggaaagaga
     5341 agaaggttag tacaattgct atagtgagtt gtattatact atgcagatat actatgccaa
     5401 tgattaattg tcaaactagg gctgcagggt tcatagtgcc acttttcctg cactgcccca
     5461 tctcctgccc accctttccc aggcatagac agtcagtgac ttaccaaact cacaggaggg
     5521 agaaggcaga agcttgagac agacccgcgg gaccgccgaa ctgcgagggg acgtggctag
     5581 ggcggcttct tttatggtgc gccggccctc ggaggcaggg cgctcgggga ggcctagcgg
     5641 ccaatctgcg gtggcaggag gcggggccga aggccgtgcc tgaccaatcc ggagcacata
     5701 ggagtctcag ccccccgccc caaagcaagg ggaagtcacg cgcctgtagc gccagcgtgt
     5761 tgtgaaatgg gggcttgggg gggttggggc cctgactagt caaaacaaac tcccattgac
     5821 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     5881 gtactgccaa aaccgcatca tcatggtaat agcgatgact aatacgtaga tgtactgcca
     5941 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     6001 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     6061 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     6121 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     6181 gccatttacc gtaagttatg taacgcctgc aggttaatta agaacatgtg agcaaaaggc
     6241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6721 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatggc tagttaatta
     6961 acatttaaat cagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     7021 ttttgtgtga atcgtaacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     7081 tagcaaaata ggctgtcccc agtgcaagtg caggtgcca
//
