LOCUS       sequence-430129-        7251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRL1 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..7251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..260
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             273..541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     591..650
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             651..677
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1785..1805
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQA"
     CDS             3696..3719
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4434..4555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4499..4518
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    4659..5053
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(5067..5441)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        6034..6337
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(6417..7005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6497..6516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7113..7161
                     /note="synthetic polyadenylation signal"
     misc_feature    join(7172..7251,1..12)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7212..7231
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gaacatttct ctatcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
       61 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
      121 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      181 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      241 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
      301 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
      361 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
      421 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
      481 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
      541 ccaagctgtg accggcgcct acctgagatc accggcgaag gagggccacc atgtacagga
      601 tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg tacccatacg
      661 atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt tggattaacg
      721 gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa gataccggca
      781 ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag gtggcggcga
      841 ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc tatgcgcaga
      901 gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg tatccgttta
      961 cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg gtggaagcgc
     1021 tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg gaagaaattc
     1081 cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt aacctgcagg
     1141 aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt aaatatgaaa
     1201 acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa gcgggcctga
     1261 cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat tatagcattg
     1321 cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg tgggcgtgga
     1381 gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc tttaaaggcc
     1441 agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg agcccgaaca
     1501 aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc ctggaagcgg
     1561 tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa gaactggcga
     1621 aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt atgccgaaca
     1681 ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac gcggcgagcg
     1741 gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac ctttacttcc
     1801 aagcccaccg cgaaatctac cagggccgca ttaacgagct cctgcttagt gtgatcacat
     1861 gggttggcat tgtgatcagc ctggtgtgcc tggccatttg tatctccact ttctgttttc
     1921 tcagaggact gcagaccgac aggaacacca ttcacaagaa cctctgcatt aacctgttcc
     1981 tcgctgagct cctgttcctg gttgggatcg acaagaccca gtacgaaatc gcttgcccca
     2041 tcttcgctgg tctcctgcat tattttttcc tggctgcctt ttcctggctg tgcctggagg
     2101 gtgtccatct ctacttgctg ttggtagagg tctttgagag cgagtattcc cgcaccaagt
     2161 actattacct gggtggctac tgtttcccag ctctggttgt gggcatcgcc gcagccatcg
     2221 attacagatc ctacgggaca gagaaggctt gttggctccg cgtcgataac tatttcattt
     2281 ggtccttcat cggtccagtt tcttttgtca tcgtcgtgaa cttggtattc ctgatggtga
     2341 cactgcacaa gatgatccgc agcagttccg tgttgaagcc cgattccagc cgtctggata
     2401 acatcaagtc ctgggcgctg ggcgccatcg cgcttttgtt tcttctgggg ttgacctggg
     2461 cctttggcct cctgttcatc aataaagaaa gcgtcgttat ggcttatctg ttcactacct
     2521 tcaacgcctt ccagggcgtg ttcattttcg ttttccattg cgcgctgcag aaaaaggtcc
     2581 acaaggaata ttccaagtgc ctgcgccata gctattgttg cattcggagc ccgccaggtg
     2641 gcacacacgg atctctgaag acatccgcga tgcgttctaa cacccggtac tataccggta
     2701 cccagtcccg cattcggcgt atgtggaacg acaccgtgcg caaacagacc gagtcctctt
     2761 ttatggctgg cgacatcaac tcaaccccga ccctcaaccg gggtaccatg ggcaaccatc
     2821 tgcttaccaa cccagtactg cagccccgtg gcggaacaag tccctacaac acccttattg
     2881 cggagtctgt ggggttcaac ccgagctcac cgcctgtctt caatagcccc ggttcttatc
     2941 gggagccgaa gcatcccctg ggtggcagag aagcctgcgg catggacacc ctgccgctta
     3001 acggtaactt caataactcc tattccctgc ggagtggcga ctttccgcct ggcgatggtg
     3061 gcccagaacc tccaaggggt cgtaacctgg ctgatgctgc cgcgttcgaa aagatgatca
     3121 ttagcgagtt ggttcacaac aatctgagag gtagctcttc cgccgcgaaa ggaccgcctc
     3181 cgcctgagcc tcccgttcca cctgtgcctg gtggaggcgg ggaggaagag gccggcggtc
     3241 caggcggggc cgacagggct gagatcgaac tgctctacaa ggcccttgaa gagcccctgc
     3301 tcctgcctcg cgcgcagtct gtgctgtatc agtccgacct ggatgagagt gaatcctgca
     3361 ccgctgaaga tggagctacc tcccgtcctc tgtccagtcc tcccggacgc gattccctgt
     3421 atgctagtgg cgcaaacctg agggatagtc ccagctatcc agactcctct cccgagggac
     3481 catctgaggc actcccacct ccaccgcctg ctccacctgg cccaccggaa atctactata
     3541 cgagtcgtcc gccagcgctg gtcgctcgca atccactgca aggttattac caggtacgcc
     3601 gtcctagcca tgagggctat cttgccgcac ccggcctgga ggggcctggc cctgacggtg
     3661 atggacagat gcagttggtc acctcactgg gttctgacta caaagacgat gacgacaagt
     3721 gaagatctga caaaactcac acatgcccac cgtgcccagc acctgaactc ctggggggac
     3781 cgtcagtctt cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg
     3841 aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt
     3901 acgtggacgg cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca
     3961 gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg
     4021 agtacaagtg caaggtctcc aacaaagccc tcccagcccc catcgagaaa accatctcca
     4081 aagccaaagg gcagccccga gaaccacagg tgtacaccct gcccccatcc cgggaggaga
     4141 tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg
     4201 ccgtggagtg ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc
     4261 tggactccga cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc
     4321 agcaggggaa cgtcttctca tgctccgtga tgcacgaggc tctgcacaac cactacacgc
     4381 agaagagcct ctccctgtct ccgggtaaat gagtgctagc tggccagaca tgataagata
     4441 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     4501 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     4561 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     4621 caagtaaaac ctctacaaat gtggtatgga attaattcta aaatacagca tagcaaaact
     4681 ttaacctcca aatcaagcct ctacttgaat ccttttctga gggatgaata aggcataggc
     4741 atcaggggct gttgccaatg tgcattagct gtttgcagcc tcaccttctt tcatggagtt
     4801 taagatatag tgtattttcc caaggtttga actagctctt catttcttta tgttttaaat
     4861 gcactgacct cccacattcc ctttttagta aaatattcag aaataattta aatacatcat
     4921 tgcaatgaaa ataaatgttt tttattaggc agaatccaga tgctcaaggc ccttcataat
     4981 atcccccagt ttagtagttg gacttaggga acaaaggaac ctttaataga aattggacag
     5041 caagaaagcg agcttctagc ttatcctcag tcctgctcct ctgccacaaa gtgcacgcag
     5101 ttgccggccg ggtcgcgcag ggcgaactcc cgcccccacg gctgctcgcc gatctcggtc
     5161 atggccggcc cggaggcgtc ccggaagttc gtggacacga cctccgacca ctcggcgtac
     5221 agctcgtcca ggccgcgcac ccacacccag gccagggtgt tgtccggcac cacctggtcc
     5281 tggaccgcgc tgatgaacag ggtcacgtcg tcccggacca caccggcgaa gtcgtcctcc
     5341 acgaagtccc gggagaaccc gagccggtcg gtccagaact cgaccgctcc ggcgacgtcg
     5401 cgcgcggtga gcaccggaac ggcactggtc aacttggcca tgatggctcc tcctgtcagg
     5461 agaggaaaga gaagaaggtt agtacaattg ctatagtgag ttgtattata ctatgcagat
     5521 atactatgcc aatgattaat tgtcaaacta gggctgcagg gttcatagtg ccacttttcc
     5581 tgcactgccc catctcctgc ccaccctttc ccaggcatag acagtcagtg acttaccaaa
     5641 ctcacaggag ggagaaggca gaagcttgag acagacccgc gggaccgccg aactgcgagg
     5701 ggacgtggct agggcggctt cttttatggt gcgccggccc tcggaggcag ggcgctcggg
     5761 gaggcctagc ggccaatctg cggtggcagg aggcggggcc gaaggccgtg cctgaccaat
     5821 ccggagcaca taggagtctc agccccccgc cccaaagcaa ggggaagtca cgcgcctgta
     5881 gcgccagcgt gttgtgaaat gggggcttgg gggggttggg gccctgacta gtcaaaacaa
     5941 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     6001 acgcccattg atgtactgcc aaaaccgcat catcatggta atagcgatga ctaatacgta
     6061 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     6121 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     6181 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     6241 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     6301 tcagccaggc gggccattta ccgtaagtta tgtaacgcct gcaggttaat taagaacatg
     6361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6841 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7081 gctagttaat taacatttaa atcagcggcc gcaataaaat atctttattt tcattacatc
     7141 tgtgtgttgg ttttttgtgt gaatcgtaac taacatacgc tctccatcaa aacaaaacga
     7201 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc a
//
