LOCUS       sequence-430134-       10057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10057)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0009
FEATURES             Location/Qualifiers
     source          1..10057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2995..3583
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3048..3068)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3466..3477)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3655..3888
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3966..4087
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4003..4022)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4057..4076
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4127..4262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4282..4301)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4283..4301)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4311..4328)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4311..4327)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4320..4342)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4469..4924
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4556..4575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4766..4787
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4950..5054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5055..5123,5124..5915)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5273..5292)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6086..6674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6575..6594
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6828..6845
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6962..6983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6998..7003,7004..7021,7022..7028)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7036..7052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7041..7063
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7060..7076
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7060..7076
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7095..7115
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7097..7115
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7143..7369
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7370..7550
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7597..7722
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8215..8448
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8633..8677
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8908..9118
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8930..9126
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8977..9112
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9158..9760
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9158..9177)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9654..9674
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9803..9920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 accattgaca tttatggatg tggccataga attctctctg gaggagtggc agtgcctgga
     1261 cacagcacag cagaatttat ataggaatgt catgttagag aattatagaa acctggtatt
     1321 cctgggtatt gctgtgtcta atttagacct gatcacctgt ctggagcaag gaaaagagcc
     1381 ctggaatatg aagagacacg agatggcagc caaaccccca gctatgtgtt ctcattttgc
     1441 caaagacctt aggccagagc aatatataaa aaattctttc caacaagtga tactgagaag
     1501 atatggaaaa tgtggatatc agaaaggctg taaaagtgtg gatgagcata agttgcacaa
     1561 aggaggtcac aagggactta accggtgtgt gacaactacc cagagcaaaa tagttcagtg
     1621 tgacaaatac gtgaaagtct ttcataaata ttcaaatgca aagagacata agataagaca
     1681 tactggaaaa aatcctttca aatgtaaaga atgtggcaaa tcattttgca tgctttcaca
     1741 actaactcaa catgagataa ttcatactgg agaaaaaccc tacaaatgtg aagaatgtgg
     1801 caaagctttt aaaaagtcct caaaccttac gaatcataag ataattcata ctggagagaa
     1861 accctacaaa tgtgaagaat gtggaaaagc ttttaaccag tcctcaactc ttactagaca
     1921 taagataatt catactggag agaaactcta caaatgtgaa gaatgtggca aagcttttaa
     1981 ccggtcctca aaccttacta aacataagat agttcatact ggagagaaac cctacaaatg
     2041 tgaagaatgt ggcaaagctt ttaaacagtc ctcaaacctt actaatcaca agaaaattca
     2101 tactggagag aaaccctaca aatgtggaga atgtggcaaa gcctttaccc tatcttcaca
     2161 ccttactaca cataagagga ttcatactgg tgagaaaccc tacaaatgtg aagaatgtgg
     2221 caaagctttt agtgtatttt caacccttac taaacataag ataattcata ctgaagagaa
     2281 accctacaaa tgtgaagaat gtggcaaagc ttttaaccgg tcctcacacc ttactaatca
     2341 taaggtaatt catactggag agaaacccta caaatgtgaa gaatgtggta aagcctttac
     2401 caagtcctca actcttactt atcataaggt aattcatact ggaaagaaac cctacaaatg
     2461 tgaagaatgt ggtaaagcct ttagtatatt ctcaatcctt actaaacata aagtaattca
     2521 tactgaagac aaaccctaca aatgtgaaga atgtggcaaa acttttaact actcctcaaa
     2581 ttttactaat cataaaaaaa ttcatactgg agagaaaccc tataagtgtg aagaatgtgg
     2641 caaaagcttt attctgtcct ctcatcttac tacacataag ataattcata ctggagagaa
     2701 accctacaaa tgtaaagaat gtggcaaagc ttttaaccag tcctcaaccc ttatgaaaca
     2761 taagataatt catactggag agaaacccta caaatgtgaa gaatgtggca aagcctttaa
     2821 ccagtcccca aaccttacta aacataagag aattcatacc aaagagaaac cctacaaatg
     2881 taaatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     2941 ttgtggaaag gacgatcgcc tcgcgcactt ggcccgatca cgcgttaagt cgacaatcaa
     3001 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3061 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3121 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3181 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3241 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3301 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3361 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3421 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3481 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3541 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     3601 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3661 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     3721 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3781 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3841 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     3901 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3961 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4021 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4081 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     4141 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4201 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4261 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4321 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4381 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4441 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4501 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4561 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4621 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4681 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4741 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4801 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4861 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4921 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5041 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5101 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5161 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5221 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5281 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5341 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5401 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5461 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5521 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5581 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5641 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5701 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5761 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5821 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5881 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6721 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6781 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6841 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6901 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6961 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7021 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7081 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     7141 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     7201 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     7261 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7321 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7381 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7441 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7501 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7561 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7621 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7681 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7741 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7801 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7861 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7921 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7981 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8041 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     8101 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     8161 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8221 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8281 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8341 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8401 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8461 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8521 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8581 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8641 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8701 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8761 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8821 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     8881 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     8941 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9001 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9061 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9121 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     9181 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     9241 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     9301 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     9361 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     9421 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     9481 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     9541 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     9601 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     9661 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     9721 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     9781 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9841 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9901 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     9961 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
    10021 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
