LOCUS       sequence-430138-        7728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..7728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..260
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             273..541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     591..650
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             651..677
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4173..4196
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4911..5032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4922..4941)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4976..4995
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    5136..5530
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(5544..5918)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        6511..6814
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(6894..7482)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6974..6993)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7590..7638
                     /note="synthetic polyadenylation signal"
     misc_feature    join(7649..7728,1..12)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7689..7708
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gaacatttct ctatcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
       61 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
      121 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      181 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      241 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
      301 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
      361 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
      421 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
      481 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
      541 ccaagctgtg accggcgcct acctgagatc accggcgaag gagggccacc atgtacagga
      601 tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg tacccatacg
      661 atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt tggattaacg
      721 gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa gataccggca
      781 ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag gtggcggcga
      841 ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc tatgcgcaga
      901 gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg tatccgttta
      961 cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg gtggaagcgc
     1021 tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg gaagaaattc
     1081 cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt aacctgcagg
     1141 aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt aaatatgaaa
     1201 acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa gcgggcctga
     1261 cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat tatagcattg
     1321 cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg tgggcgtgga
     1381 gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc tttaaaggcc
     1441 agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg agcccgaaca
     1501 aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc ctggaagcgg
     1561 tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa gaactggcga
     1621 aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt atgccgaaca
     1681 ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac gcggcgagcg
     1741 gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac ctttacttcc
     1801 aagatgcaag cccaagggaa cgcttggagg gggacctgga actgctggcc gtatttacac
     1861 atgtagttgt ggccgtatcc gtagccgccc tggtgcttac cgcggcgatt ctgctttcct
     1921 tgcgcagtct gaaaagcaat gttcggggca tacacgccaa cgttgctgcg gctcttgggg
     1981 tagccgaact cctgtttctc cttgggattc atcgaacgca caatcagctt gtttgcacgg
     2041 cggttgccat cctccttcat tatttctttc ttagcacctt cgcttggctg tttgtgcagg
     2101 ggcttcatct gtaccgaatg caagttgagc ctaggaacgt agataggggt gcaatgaggt
     2161 tttatcatgc cttggggtgg ggtgtgcccg ctgtcctcct tggacttgcg gtggggttgg
     2221 acccggaggg ctacggaaac cctgacttct gttggattag tgttcatgag ccgctcatat
     2281 ggtcttttgc tggtccggta gtcttggtga ttgtaatgaa cggtactatg ttccttctgg
     2341 ctgctcgcac ctcttgtagt actggccaaa gggaagcaaa aaagacgagc gcgctgacat
     2401 tgcgatcaag ctttcttttg ctgcttcttg taagcgcgtc ctggttgttt ggtttgctgg
     2461 ccgtcaacca ttcaattctg gcctttcact atctgcacgc agggttgtgt ggactccaag
     2521 gattggcagt gcttctgctg ttctgcgttc ttaacgccga cgccagagca gcatggatgc
     2581 cggcttgcct tggacgcaag gctgcaccag aagaggctag accggcacca ggcctgggac
     2641 ctggggcata caacaacacc gccttgttcg aagaaagtgg gcttatacgg ataactcttg
     2701 gcgcgtcaac tgtttcaagt gtaagctcag cacggtctgg ccggacccaa gatcaagact
     2761 cacaacgcgg ccgcagttac cttagggata acgttctcgt gaggcatggt agcgcagctg
     2821 accacaccga tcattctctc caagcccatg ctggacccac tgacctcgac gttgcgatgt
     2881 tccaccgcga cgctggtgcc gacagtgata gcgattccga cttgtcactt gaagaggagc
     2941 ggtctctttc tattccatct tcagaaagtg aagacaacgg tagaaccagg ggtcggttcc
     3001 agagaccgct ctgtcgcgcc gcgcaatcag agagactctt gacccatcct aaggacgtag
     3061 acggaaacga tctgttgtct tattggcctg ctcttggaga gtgtgaggca gcaccatgtg
     3121 cacttcaaac atggggaagt gagcgccggc ttggcctcga tacgagcaaa gacgccgcga
     3181 acaataatca gcctgacccg gctcttacta gcggtgatga aacatctctc ggtagggcac
     3241 aacgacaacg caaaggcatt cttaagaatc gcttgcagta tccactggtg cctcagaccc
     3301 gcggagcacc ggagctttct tggtgccggg cagcgactct cggccaccgg gcggtacccg
     3361 cggcgtccta cggccggatt tatgcgggag gaggcacggg aagcctctcc cagcctgcat
     3421 cacgctattc aagccgagaa cagttggacc tcctgctcag aagacaactg tcacgcgaac
     3481 ggcttgaaga agccccagcg cctgtcttga gacctttgag caggcccggc tctcaggagt
     3541 gcatggacgc ggcgcccggg cgactggagc ctaaagacag gggcagtacg cttccacggc
     3601 gacaaccacc cagagactac cctggggcca tggcagggcg gtttggcagt agagacgcgc
     3661 ttgacctggg cgctccaaga gagtggctct ccacacttcc acctccgcgc cgaacacgag
     3721 atctcgaccc ccaaccaccc ccgcttcccc tgtcaccaca acggcaactc tctcgcgatc
     3781 cgcttctccc ctcaaggccg cttgactcac tcagtaggtc cagcaatagt agagaacaac
     3841 tcgaccaagt accttctcgc cacccgtcaa gggaggccct gggtccgctg ccccagctct
     3901 tgagagcgag agaagactcc gtctcaggcc cgtcccatgg tccctctaca gagcaactgg
     3961 atatactgtc ctccatcctt gcttccttca atagttcagc cctgtcttct gttcagtcca
     4021 gcagcacacc gcttggcccc cacaccacag caactccttc cgcgacggcc tccgttttgg
     4081 gaccctccac tcctcgatca gcaacgtcac actcaatctc tgagctctct cccgattccg
     4141 aagttccccg atcagaaggg cactctggtt ctgactacaa agacgatgac gacaagtgaa
     4201 gatctgacaa aactcacaca tgcccaccgt gcccagcacc tgaactcctg gggggaccgt
     4261 cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg acccctgagg
     4321 tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc aactggtacg
     4381 tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag tacaacagca
     4441 cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat ggcaaggagt
     4501 acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc atctccaaag
     4561 ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg gaggagatga
     4621 ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc gacatcgccg
     4681 tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct cccgtgctgg
     4741 actccgacgg ctccttcttc ctctacagca agctcaccgt ggacaagagc aggtggcagc
     4801 aggggaacgt cttctcatgc tccgtgatgc acgaggctct gcacaaccac tacacgcaga
     4861 agagcctctc cctgtctccg ggtaaatgag tgctagctgg ccagacatga taagatacat
     4921 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     4981 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     5041 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     5101 gtaaaacctc tacaaatgtg gtatggaatt aattctaaaa tacagcatag caaaacttta
     5161 acctccaaat caagcctcta cttgaatcct tttctgaggg atgaataagg cataggcatc
     5221 aggggctgtt gccaatgtgc attagctgtt tgcagcctca ccttctttca tggagtttaa
     5281 gatatagtgt attttcccaa ggtttgaact agctcttcat ttctttatgt tttaaatgca
     5341 ctgacctccc acattccctt tttagtaaaa tattcagaaa taatttaaat acatcattgc
     5401 aatgaaaata aatgtttttt attaggcaga atccagatgc tcaaggccct tcataatatc
     5461 ccccagttta gtagttggac ttagggaaca aaggaacctt taatagaaat tggacagcaa
     5521 gaaagcgagc ttctagctta tcctcagtcc tgctcctctg ccacaaagtg cacgcagttg
     5581 ccggccgggt cgcgcagggc gaactcccgc ccccacggct gctcgccgat ctcggtcatg
     5641 gccggcccgg aggcgtcccg gaagttcgtg gacacgacct ccgaccactc ggcgtacagc
     5701 tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt ccggcaccac ctggtcctgg
     5761 accgcgctga tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg
     5821 aagtcccggg agaacccgag ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc
     5881 gcggtgagca ccggaacggc actggtcaac ttggccatga tggctcctcc tgtcaggaga
     5941 ggaaagagaa gaaggttagt acaattgcta tagtgagttg tattatacta tgcagatata
     6001 ctatgccaat gattaattgt caaactaggg ctgcagggtt catagtgcca cttttcctgc
     6061 actgccccat ctcctgccca ccctttccca ggcatagaca gtcagtgact taccaaactc
     6121 acaggaggga gaaggcagaa gcttgagaca gacccgcggg accgccgaac tgcgagggga
     6181 cgtggctagg gcggcttctt ttatggtgcg ccggccctcg gaggcagggc gctcggggag
     6241 gcctagcggc caatctgcgg tggcaggagg cggggccgaa ggccgtgcct gaccaatccg
     6301 gagcacatag gagtctcagc cccccgcccc aaagcaaggg gaagtcacgc gcctgtagcg
     6361 ccagcgtgtt gtgaaatggg ggcttggggg ggttggggcc ctgactagtc aaaacaaact
     6421 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
     6481 cccattgatg tactgccaaa accgcatcat catggtaata gcgatgacta atacgtagat
     6541 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
     6601 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
     6661 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
     6721 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
     6781 gccaggcggg ccatttaccg taagttatgt aacgcctgca ggttaattaa gaacatgtga
     6841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     6901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     6961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7021 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7081 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7321 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7381 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7441 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7501 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatggct
     7561 agttaattaa catttaaatc agcggccgca ataaaatatc tttattttca ttacatctgt
     7621 gtgttggttt tttgtgtgaa tcgtaactaa catacgctct ccatcaaaac aaaacgaaac
     7681 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgcca
//
