LOCUS       sequence-430139-        7317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRB1 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..7317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        49..260
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             273..541
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     591..650
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             651..677
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3762..3785
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    4500..4621
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4511..4530)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4565..4584
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    4725..5119
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(5133..5507)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        6100..6403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(6483..7071)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6563..6582)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7179..7227
                     /note="synthetic polyadenylation signal"
     misc_feature    join(7238..7317,1..12)
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7278..7297
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 gaacatttct ctatcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
       61 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
      121 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      181 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      241 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
      301 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
      361 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
      421 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
      481 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
      541 ccaagctgtg accggcgcct acctgagatc accggcgaag gagggccacc atgtacagga
      601 tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg tacccatacg
      661 atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt tggattaacg
      721 gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa gataccggca
      781 ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag gtggcggcga
      841 ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc tatgcgcaga
      901 gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg tatccgttta
      961 cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg gtggaagcgc
     1021 tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg gaagaaattc
     1081 cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt aacctgcagg
     1141 aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt aaatatgaaa
     1201 acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa gcgggcctga
     1261 cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat tatagcattg
     1321 cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg tgggcgtgga
     1381 gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc tttaaaggcc
     1441 agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg agcccgaaca
     1501 aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc ctggaagcgg
     1561 tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa gaactggcga
     1621 aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt atgccgaaca
     1681 ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac gcggcgagcg
     1741 gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac ctttacttcc
     1801 aacagctgag cgccgatgct aacatggaga aggctacact gcccagtgtc acactgatcg
     1861 tgggttgtgg agtgagctct ctcacgctcc tgatgctggt catcatttat gtgagcgtgt
     1921 ggcgctatat tcgcagtgaa cgcagcgtca ttctgatcaa cttttgcctt agcattatct
     1981 caagcaacgc gctgatcctc atcggccaaa cgcagacacg taacaaggtt gtatgtacac
     2041 tggtggccgc attcctccat ttctttttcc tgtcttcctt ctgttgggtt ttgactgagg
     2101 cctggcaatc ctacatggct gtgaccgggc atctgcgtaa ccgtttgatc cggaagcggt
     2161 ttctgtgctt gggatgggga ttgcccgctc tggtcgtggc tattagtgta ggtttcacaa
     2221 aggcgaaggg ttatagcacc atgaactatt gctggctgtc tctcgaagga ggcttgctgt
     2281 atgcttttgt gggacccgct gccgctgttg tcctggtcaa catggtgatt ggtattctgg
     2341 tgtttaataa gctcgtatct aaggacggca ttaccgacaa aaagctgaag gaacgcgctg
     2401 gagccagcct ctggtccagt tgcgtcgtgc tcccacttct cgcactcacg tggatgagcg
     2461 ccgtcctcgc cgtgaccgac cggcgctccg ccctgtttca aattctgttc gcagtgtttg
     2521 actctctgga gggcttcgtt atcgtgatgg tccactgtat ccttaggcgt gaggtgcagg
     2581 atgctgtgaa gtgccgtgtc gtagacaggc aggaagaggg taatggcgat tccggtggct
     2641 ctttccagaa cggtcacgcc cagcttatga cagacttcga gaaggacgtg gatcttgcgt
     2701 gccgctctgt gctcaacaag gacatcgcag cgtgtcggac tgcaaccatc accggcaccc
     2761 tgaaaagacc tagcctcccg gaagaggaaa agctgaagct ggctcatgcg aagggaccgc
     2821 ccacaaattt taattctctg cctgctaatg tcagtaagct ccacctgcac ggcagcccac
     2881 gctaccccgg tggccctctc cccgattttc ctaaccactc actgaccctg aaacgggata
     2941 aggctcccaa aagctctttc gtgggagacg gtgacatctt taaaaagctc gatagcgagc
     3001 tgagccgtgc ccaagagaag gccctggata catcttacgt gattctccca acagccacag
     3061 ccacgctgcg cccaaagcct aaggaagagc caaaatactc tattcacatc gatcagatgc
     3121 cccagaccag acttatccac ctgtccactg cccctgaggc aagtctgccc gcgcgcagcc
     3181 caccttctag acagccgccc agtggtggcc cgcctgaagc accgccagcg caaccgccac
     3241 cgcctccacc tccgcctcca ccgcctccgc agcaaccact gccacctccg ccaaaccttg
     3301 aacctgcccc tccgtccctg ggtgatcccg gagagccggc ggcccacccc ggtccctcaa
     3361 ccggaccctc caccaagaat gagaacgttg ccaccttgtc cgtttcctca ttggaaagga
     3421 gaaagtctag atatgccgaa ctcgactttg agaagattat gcacacccgg aagcgccatc
     3481 aggacatgtt ccaggacctg aaccggaaac tccagcacgc cgctgaaaaa gacaaagagg
     3541 tgctgggtcc ggactcaaag ccagagaaac agcaaactcc caataagagg ccttgggagt
     3601 ccctgcgcaa ggcacatgga accccaacct gggttaaaaa ggagcttgag cctcttcagc
     3661 caagcccact cgaactgcgc tccgtcgagt gggagagatc tggcgcgact atcccactcg
     3721 tcggccagga tattatcgac ctgcaaacag aggtgggttc tgactacaaa gacgatgacg
     3781 acaagtgaag atctgacaaa actcacacat gcccaccgtg cccagcacct gaactcctgg
     3841 ggggaccgtc agtcttcctc ttccccccaa aacccaagga caccctcatg atctcccgga
     3901 cccctgaggt cacatgcgtg gtggtggacg tgagccacga agaccctgag gtcaagttca
     3961 actggtacgt ggacggcgtg gaggtgcata atgccaagac aaagccgcgg gaggagcagt
     4021 acaacagcac gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg
     4081 gcaaggagta caagtgcaag gtctccaaca aagccctccc agcccccatc gagaaaacca
     4141 tctccaaagc caaagggcag ccccgagaac cacaggtgta caccctgccc ccatcccggg
     4201 aggagatgac caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg
     4261 acatcgccgt ggagtgggag agcaatgggc agccggagaa caactacaag accacgcctc
     4321 ccgtgctgga ctccgacggc tccttcttcc tctacagcaa gctcaccgtg gacaagagca
     4381 ggtggcagca ggggaacgtc ttctcatgct ccgtgatgca cgaggctctg cacaaccact
     4441 acacgcagaa gagcctctcc ctgtctccgg gtaaatgagt gctagctggc cagacatgat
     4501 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4561 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4621 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     4681 ttaaagcaag taaaacctct acaaatgtgg tatggaatta attctaaaat acagcatagc
     4741 aaaactttaa cctccaaatc aagcctctac ttgaatcctt ttctgaggga tgaataaggc
     4801 ataggcatca ggggctgttg ccaatgtgca ttagctgttt gcagcctcac cttctttcat
     4861 ggagtttaag atatagtgta ttttcccaag gtttgaacta gctcttcatt tctttatgtt
     4921 ttaaatgcac tgacctccca cattcccttt ttagtaaaat attcagaaat aatttaaata
     4981 catcattgca atgaaaataa atgtttttta ttaggcagaa tccagatgct caaggccctt
     5041 cataatatcc cccagtttag tagttggact tagggaacaa aggaaccttt aatagaaatt
     5101 ggacagcaag aaagcgagct tctagcttat cctcagtcct gctcctctgc cacaaagtgc
     5161 acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg ctcgccgatc
     5221 tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg
     5281 gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc cggcaccacc
     5341 tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg
     5401 tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac cgctccggcg
     5461 acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatgat ggctcctcct
     5521 gtcaggagag gaaagagaag aaggttagta caattgctat agtgagttgt attatactat
     5581 gcagatatac tatgccaatg attaattgtc aaactagggc tgcagggttc atagtgccac
     5641 ttttcctgca ctgccccatc tcctgcccac cctttcccag gcatagacag tcagtgactt
     5701 accaaactca caggagggag aaggcagaag cttgagacag acccgcggga ccgccgaact
     5761 gcgaggggac gtggctaggg cggcttcttt tatggtgcgc cggccctcgg aggcagggcg
     5821 ctcggggagg cctagcggcc aatctgcggt ggcaggaggc ggggccgaag gccgtgcctg
     5881 accaatccgg agcacatagg agtctcagcc ccccgcccca aagcaagggg aagtcacgcg
     5941 cctgtagcgc cagcgtgttg tgaaatgggg gcttgggggg gttggggccc tgactagtca
     6001 aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg agtcaaaccg
     6061 ctatccacgc ccattgatgt actgccaaaa ccgcatcatc atggtaatag cgatgactaa
     6121 tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca
     6181 ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg
     6241 atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt
     6301 ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt
     6361 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcctgcag gttaattaag
     6421 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6481 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6541 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6601 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6661 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6721 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6781 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6841 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6901 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     6961 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7021 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7081 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7141 gtcatggcta gttaattaac atttaaatca gcggccgcaa taaaatatct ttattttcat
     7201 tacatctgtg tgttggtttt ttgtgtgaat cgtaactaac atacgctctc catcaaaaca
     7261 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgcca
//
