LOCUS       sequence-430148-       13108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2886
FEATURES             Location/Qualifiers
     source          1..13108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8965..9553
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9018..9038)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9436..9447)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9625..9858
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9936..10057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9973..9992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10027..10046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10097..10232
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10252..10271)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10253..10271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10281..10298)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10281..10297)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10290..10312)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10439..10894
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10526..10545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10736..10757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10920..11024
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11025..11093,11094..11885)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11243..11262)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12056..12644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12545..12564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12798..12815
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12932..12953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12968..12973,12974..12991,12992..12998)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13006..13022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13011..13033
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13030..13046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13030..13046
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13065..13085
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        13067..13085
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gcccagtccc acagctccac
     4141 gaccactgaa aagaaaaaag ttgagaattc catagtgaag tgctccactc gaacagatgt
     4201 gagcgagaaa gccgttgcct ccagcaccac ttctaatgag gatgaaagtc ctggacagac
     4261 ttatcacaga gagagaagaa acgcaatcac tatgcagcca cagaatgtcc aggggctcag
     4321 caaagtcagt gaggaacctt caacatcgag tgacgagagg gcctcattga tcaagaaaga
     4381 gatccatggg tccctgccac acgtggcgga gccctctgtg ccgtaccgcg ggacggtgtt
     4441 tgccatggac cccaggaatg gttacatgga gccccactac caccctcctc atcttttccc
     4501 tgccttccat cctcctgtac caattgatgc cagacatcat gagggccgtt accattacga
     4561 tccatctccg attcctccat tgcatatgac ttccgcctta tctagtagcc ctacgtatcc
     4621 ggacctgccc ttcattagga tctccccaca ccggaacccc actgctgctt ccgagtctcc
     4681 cttcagccct ccacatccct acattaatcc ctacatggac tatatccgct ccttgcacag
     4741 cagcccatcg ctctccatga tctcagcaac ccgtgggctg agccctacag atgcgcccca
     4801 tgcaggagtc agcccagcag aatactatca tcagatggcc ctgctaactg gccagcgcag
     4861 cccctatgca gacattattc cctcagctgc caccgccggc acgggggcca tccacatgga
     4921 atatcttcat gctatggata gcaccagatt ctccagcccc aggctgtcag ccaggccgag
     4981 ccgaaaacgt acactgtcca tatcaccact ctccgatcat agctttgacc ttcagaccat
     5041 gataaggacg tctcccaact ccttggtcac gattctcaat aattcccgta gcagctcttc
     5101 agcaagtggc tcctatggtc acttatctgc aagtgcaatc agccctgcct tgagcttcac
     5161 ctactcttcc gcgcccgtct ctctccacat gcatcagcag atcctaagcc gacaacagag
     5221 cttaggttca gcctttggac acagccctcc actcatccac cctgccccaa cttttccaac
     5281 acagaggcct attccaggga tccctacggt tctgaacccc gtccaggtca gctccggccc
     5341 ttctgagtcc tcacagaaca agcccacgag tgagtctgca gtgagcagca ctggtgaccc
     5401 gatgcacaac aagaggtcca agatcaaacc cgatgaagac ctccccagcc caggggctcg
     5461 ggggcagcag gaacagcccg aaggaacaac ccttgtcaag gaggaagggg acaaagatga
     5521 aagcaaacag gagcctgaag tcatctatga gacaaactgc cactgggaag gctgcgcgag
     5581 ggagttcgac acccaagagc agcttgtgca ccatataaat aacgaccata ttcatggaga
     5641 gaagaaggag ttcgtgtgca ggtggctgga ctgctcaaga gagcagaaac ccttcaaagc
     5701 ccagtatatg ttggtagtgc atatgagaag acacacgggc gagaagcctc acaaatgcac
     5761 ttttgaaggt tgcacaaagg cctactcgag actagaaaac ttgaaaacac acttgagatc
     5821 tcacactgga gagaaaccat acgtctgtga gcacgaaggt tgcaacaagg ctttctcaaa
     5881 tgcctctgat cgcgccaaac accaaaacag aacgcattcc aatgagaaac catatgtgtg
     5941 caaaatccca ggctgcacta agcgttacac agacccaagc tccctccgga aacatgtgaa
     6001 gacagtgcat ggcccagagg ctcatgtcac caagaagcag cgaggggaca tccatcctcg
     6061 gccgccaccc ccgagagatt ccggcagcca ttcacagtcc aggtcgcctg gccgaccgac
     6121 tcagggagcc cttggtgagc agcaggacct cagcaacact acctcaaagc gggaagaatg
     6181 cctccaggtg aaaaccgtca aggcagagaa gccaatgaca tctcagccaa gccctggtgg
     6241 tcagtcttca tgcagcagcc aacagtcccc catcagcaac tattccaaca gtgggctcga
     6301 gcttcctctg accgatggag gtagtatagg agacctcagt gccatcgatg aaaccccaat
     6361 catggactca accatttcca ctgcaaccac agcccttgct ttgcaagcca ggagaaaccc
     6421 ggcagggacc aaatggatgg agcacgtaaa actagaaagg ctaaaacaag tgaatggaat
     6481 gtttccgcga ctgaacccca ttctaccccc taaagcccct gcggtctctc ctctcatagg
     6541 aaatggcaca cagtccaaca acacctgcag cttgggtggg cccatgacgc ttctcccggg
     6601 cagaagcgac ctctctgggg tggacgtcac tatgctgaac atgctcaaca gaagggacag
     6661 cagcgccagc accatcagct cggcctacct gagcagccgc cgctcctcag ggatctcgcc
     6721 ctgcttctcc agccgccgct ccagcgaggc gtcacaggcc gagggccggc cgcagaacgt
     6781 gagcgtggcc gactcctacg accccatctc caccgacgcc tcgcgccgct ccagcgaagc
     6841 cagccagagc gacggcctgc ccagcctgct cagcctcacg cccgcccagc agtaccgcct
     6901 caaggccaag tacgcggctg ccacaggagg gccgccgccg acgcccctgc ccaacatgga
     6961 gaggatgagc ctgaagacgc gcctggcgct gctcggggat gccctcgagc ctggcgtggc
     7021 cctgcctcca gttcatgccc cgaggaggtg cagcgacggg ggagcccacg gctacgggcg
     7081 gcgccacctg cagccgcacg atgcgccggg ccacggcgtg aggagggcca gcgacccggt
     7141 gcggacaggc tccgagggcc tggccctgcc tcgtgtgccg cgcttcagca gcctcagcag
     7201 ctgcaacccc ccggcgatgg ccacgtccgc ggagaagcgc agtctcgtgc ttcagaatta
     7261 cacgcggccc gagggcggcc agtcccgaaa cttccactcg tccccctgtc ctcccagcat
     7321 caccgagaac gtcaccctgg agtccctgac catggacgct gatgccaacc tgaacgatga
     7381 ggatttcctg ccggacgacg tggtgcagta tttaaattcc cagaaccaag cagggtacga
     7441 gcagcacttc cccagcgccc tcccggacga cagcaaagtg ccccacgggc ccggtgactt
     7501 tgacgcgccc gggctgccag acagccacgc tggccagcag ttccatgccc tcgagcagcc
     7561 ctgccccgag ggcagcaaaa ccgacctgcc cattcagtgg aacgaagtca gctccggaag
     7621 cgccgacctg tcctcctcca agctcaagtg tgggccgcgg cccgctgtgc cgcagactcg
     7681 cgcctttggg ttctgcaacg gcatggtcgt ccacccgcag aaccccttga ggagcgggcc
     7741 tgctgggggc tatcagaccc tcggggagaa cagcaacccc tacggtggcc cagagcactt
     7801 gatgctccac aacagccccg gaagtggcac cagtggaaac gccttccatg aacagccctg
     7861 taaggccccg cagtatggga actgtctcaa caggcagcca gtggcccctg gtgcactcga
     7921 cggtgcctgt ggtgccggga ttcaagcctc aaagctgaag agcaccccca tgcaagggag
     7981 cgggggccag ctgaatttcg gcctgccggt agcgccaaat gagtcagctg gcagcatggt
     8041 gaatggcatg cagaaccagg acccagtggg acaggggtac ctggctcacc agctcctcgg
     8101 cgacagcatg cagcacccgg gggcaggccg ccccggtcag cagatgcttg ggcagattag
     8161 tgctacctca cacatcaaca tctaccaagg gccagagagc tgcctgccag gggctcacgg
     8221 catgggcagc cagccgtcaa gcttggcagt tgtcaggggc taccagccat gtgccagctt
     8281 tgggggcagc aggcgccagg ctatgccgag ggacagcctt gctctgcagt caggacagct
     8341 cagtgacaca agtcagacct gcagggtgaa tggtatcaag atggagatga aagggcagcc
     8401 ccatccgctg tgctctaatc tgcagaatta ctctggtcag ttctatgacc aaaccgtggg
     8461 cttcagtcag caagacacga aagctggttc attctctatt tcagacgcca gctgcctgct
     8521 acaggggacc agcgccaaaa actctgagtt actttcccca ggtgctaatc aggtgacaag
     8581 cacagtggac agcctcgaca gccatgacct ggaaggggta cagattgact tcgatgccat
     8641 catagacgat ggggaccact ccagcctgat gtcgggggcc ctgagcccaa gtatcattca
     8701 gaacctttcc catagctcct cccgcctcac cacgcctcgg gcgtccctcc cattcccagc
     8761 gctgtccatg agcaccacca acatggctat cggggacatg agttctttgc tgacctccct
     8821 agcggaagaa agcaaattcc ttgcagttat gcaatagtaa tgaactagtc cgtaacttga
     8881 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgacacaa ttgccataat
     8941 cggaactgta cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     9001 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     9061 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     9121 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     9181 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     9241 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     9301 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     9361 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     9421 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     9481 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     9541 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     9601 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     9661 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9721 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9781 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9841 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     9901 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     9961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10021 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
    10081 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
    10141 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
    10201 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
    10261 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    10321 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    10381 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
    10441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    10501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    10561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    10621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    10681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    10741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    10801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    10861 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    10921 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10981 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    11041 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    11101 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    11161 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    11221 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    11281 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    11341 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    11401 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    11461 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    11521 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    11581 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    11641 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    11701 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    11761 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    11821 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11881 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11941 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    12001 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    12061 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    12121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    12181 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    12241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    12301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    12361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    12421 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    12481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    12541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    12601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    12661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    12721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    12781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    12841 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    12901 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    12961 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    13021 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    13081 aaagggaaca aaagctggag ctgcaagc
//
