LOCUS       sequence-430201-        9877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral dual expression of YAP5SA and eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Burgess CL, Huang J, Bawa PS, Alysandratos KD, Minakin K, Ayers LJ, 
            Morley MP, Babu A, Villacorta-Martin C, Yampolskaya M, Hinds A, 
            Thapa BR, Wang F, Matschulat A, Mehta P, Morrisey EE, Varelas X, 
            Kotton DN
  TITLE     Generation of human alveolar epithelial type I cells from 
            pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017.
  PUBMED    38642558
REFERENCE   2  (bases 1 to 9877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017."
COMMENT     Sequence Label: p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP
FEATURES             Location/Qualifiers
     source          1..9877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3480..3545
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3549..3573
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3570..3579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5092..5116)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5127..5525
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             join(5534..5536,5537..5539,5540..6253)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5534..5536,5537..5539,5540..6250)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5579..5600)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5840..5859)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6187..6208
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6267..6855
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6320..6340)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6738..6749)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6930..7163
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7282..7300)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7368..7472
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7473..7541,7542..8333)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7691..7710)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8504..9092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8993..9012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9246..9263
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9503..9524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9539..9544,9545..9562,9563..9569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9577..9593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9582..9604
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9601..9617
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9601..9617
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9667..9686
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc aattctgcag
     3421 tcgacggtac cgcgggcccg ggatccgacc ctaggccgac gcgtctcgag ggtaccatgg
     3481 actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg
     3541 acaagcttac aagtttgtac aaaaaagttg ccaccatgga tcccgggcag cagccgccgc
     3601 ctcaaccggc cccccagggc caagggcagc cgccttcgca gcccccgcag gggcagggcc
     3661 cgccgtccgg acccgggcaa ccggcacccg cggcgaccca ggcggcgccg caggcacccc
     3721 ccgccgggca tcagatcgtg cacgtccgcg gggacgcgga gaccgacctg gaggcgctct
     3781 tcaacgccgt catgaacccc aagacggcca acgtgcccca gaccgtgccc atgaggctcc
     3841 ggaagctgcc cgactccttc ttcaagccgc cggagcccaa atcccactcc cgacaggccg
     3901 ctactgatgc aggcactgca ggagccctga ctccacagca tgttcgagct catgcctctc
     3961 cagcttctct gcagttggga gctgtttctc ctgggacact gacccccact ggagtagtct
     4021 ctggcccagc agctacaccc acagctcagc atcttcgaca gtctgctttt gagatacctg
     4081 atgatgtacc tctgccagca ggttgggaga tggcaaagac atcttctggt cagagatact
     4141 tcttaaatca catcgatcag acaacaacat ggcaggaccc caggaaggcc atgctgtccc
     4201 agatgaacgt cacagccccc accagtccac cagtgcagca gaatatgatg aactcggctt
     4261 caggtcctct tcctgatgga tgggaacaag ccatgactca ggatggagaa atttactata
     4321 taaaccataa gaacaagacc acctcttggc tagacccaag gcttgaccct cgttttgcca
     4381 tgaaccagag aatcagtcag agtgctccag tgaaacagcc accacccctg gctccccaga
     4441 gcccacaggg aggcgtcatg ggtggcagca actccaacca gcagcaacag atgcgactgc
     4501 agcaactgca gatggagaag gagaggctgc ggctgaaaca gcaagaactg cttcggcagg
     4561 caatgcggaa tatcaatccc agcacagcaa attctccaaa atgtcaggag ttagccctgc
     4621 gtagccagtt accaacactg gagcaggatg gtgggactca aaatccagtg tcttctcccg
     4681 ggatgtctca ggaattgaga acaatgacga ccaatagctc agatcctttc cttaacagtg
     4741 gcacctatca ctctcgagat gaggctacag acagtggact aagcatgagc agctacagtg
     4801 tccctcgaac cccagatgac ttcctgaaca gtgtggatga gatggataca ggtgatacta
     4861 tcaaccaaag caccctgccc tcacagcaga accgtttccc agactacctt gaagccattc
     4921 ctgggacaaa tgtggacctt ggaacactgg aaggagatgg aatgaacata gaaggagagg
     4981 agctgatgcc aagtctgcag gaagctttga gttctgacat ccttaatgac atggagtctg
     5041 ttttggctgc caccaagcta gataaagaaa gctttcttac atggttataa tacccaactt
     5101 tcttgtacaa agtggtgtga agatccggcc tccgcgccgg gttttggcgc ctcccgcggg
     5161 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     5221 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     5281 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     5341 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     5401 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     5461 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     5521 cttggccgcc catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5581 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     5641 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     5701 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     5761 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     5821 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     5881 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     5941 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     6001 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     6061 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     6121 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     6181 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     6241 gctgtacaag taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     6301 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6361 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6421 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6481 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6541 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6601 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6661 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6721 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6781 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6841 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
     6901 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6961 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7141 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7201 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
     7261 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     7321 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7381 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7441 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7501 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7561 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7621 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7681 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     7741 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7801 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7861 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7921 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7981 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8041 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8101 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8161 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8221 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8281 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8341 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8401 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8461 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8521 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8581 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8641 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8701 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8761 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8821 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8881 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8941 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9001 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9061 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9121 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9181 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9241 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9301 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
     9361 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9421 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
     9481 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9541 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9601 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
     9661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     9721 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     9781 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     9841 attgtatttg ttaaatatgt actacaaact tagtagt
//
