LOCUS       sequence-430237-        9866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral dual expression of YAP5SA and tagBFP with loxP site for 
            Cre excision.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Burgess CL, Huang J, Bawa PS, Alysandratos KD, Minakin K, Ayers LJ, 
            Morley MP, Babu A, Villacorta-Martin C, Yampolskaya M, Hinds A, 
            Thapa BR, Wang F, Matschulat A, Mehta P, Morrisey EE, Varelas X, 
            Kotton DN
  TITLE     Generation of human alveolar epithelial type I cells from 
            pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017.
  PUBMED    38642558
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017."
COMMENT     Sequence Label: p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP
FEATURES             Location/Qualifiers
     source          1..9866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3480..3545
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3549..3573
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3570..3579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5092..5116)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5127..5525
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             5540..6241
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    6260..6848
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6313..6333)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6731..6742)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    6984..7017
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7044..7224
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7271..7289)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7357..7461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7462..7530,7531..8322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7680..7699)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8493..9081
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9235..9252
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9492..9513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9528..9533,9534..9551,9552..9558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9566..9582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9571..9593
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9590..9606
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9590..9606
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9656..9675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc aattctgcag
     3421 tcgacggtac cgcgggcccg ggatccgacc ctaggccgac gcgtctcgag ggtaccatgg
     3481 actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg
     3541 acaagcttac aagtttgtac aaaaaagttg ccaccatgga tcccgggcag cagccgccgc
     3601 ctcaaccggc cccccagggc caagggcagc cgccttcgca gcccccgcag gggcagggcc
     3661 cgccgtccgg acccgggcaa ccggcacccg cggcgaccca ggcggcgccg caggcacccc
     3721 ccgccgggca tcagatcgtg cacgtccgcg gggacgcgga gaccgacctg gaggcgctct
     3781 tcaacgccgt catgaacccc aagacggcca acgtgcccca gaccgtgccc atgaggctcc
     3841 ggaagctgcc cgactccttc ttcaagccgc cggagcccaa atcccactcc cgacaggccg
     3901 ctactgatgc aggcactgca ggagccctga ctccacagca tgttcgagct catgcctctc
     3961 cagcttctct gcagttggga gctgtttctc ctgggacact gacccccact ggagtagtct
     4021 ctggcccagc agctacaccc acagctcagc atcttcgaca gtctgctttt gagatacctg
     4081 atgatgtacc tctgccagca ggttgggaga tggcaaagac atcttctggt cagagatact
     4141 tcttaaatca catcgatcag acaacaacat ggcaggaccc caggaaggcc atgctgtccc
     4201 agatgaacgt cacagccccc accagtccac cagtgcagca gaatatgatg aactcggctt
     4261 caggtcctct tcctgatgga tgggaacaag ccatgactca ggatggagaa atttactata
     4321 taaaccataa gaacaagacc acctcttggc tagacccaag gcttgaccct cgttttgcca
     4381 tgaaccagag aatcagtcag agtgctccag tgaaacagcc accacccctg gctccccaga
     4441 gcccacaggg aggcgtcatg ggtggcagca actccaacca gcagcaacag atgcgactgc
     4501 agcaactgca gatggagaag gagaggctgc ggctgaaaca gcaagaactg cttcggcagg
     4561 caatgcggaa tatcaatccc agcacagcaa attctccaaa atgtcaggag ttagccctgc
     4621 gtagccagtt accaacactg gagcaggatg gtgggactca aaatccagtg tcttctcccg
     4681 ggatgtctca ggaattgaga acaatgacga ccaatagctc agatcctttc cttaacagtg
     4741 gcacctatca ctctcgagat gaggctacag acagtggact aagcatgagc agctacagtg
     4801 tccctcgaac cccagatgac ttcctgaaca gtgtggatga gatggataca ggtgatacta
     4861 tcaaccaaag caccctgccc tcacagcaga accgtttccc agactacctt gaagccattc
     4921 ctgggacaaa tgtggacctt ggaacactgg aaggagatgg aatgaacata gaaggagagg
     4981 agctgatgcc aagtctgcag gaagctttga gttctgacat ccttaatgac atggagtctg
     5041 ttttggctgc caccaagcta gataaagaaa gctttcttac atggttataa tacccaactt
     5101 tcttgtacaa agtggtgtga agatccggcc tccgcgccgg gttttggcgc ctcccgcggg
     5161 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     5221 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     5281 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     5341 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     5401 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     5461 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     5521 cttggccgcc catatggcca tgagcgagct gattaaggag aacatgcaca tgaagctgta
     5581 catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc
     5641 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     5701 cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca
     5761 gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac
     5821 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     5881 cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca
     5941 gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct
     6001 ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat
     6061 caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta
     6121 tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca
     6181 cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatta
     6241 aaggatatcg atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     6301 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     6361 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     6421 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     6481 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     6541 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     6601 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     6661 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     6721 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     6781 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6841 ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6901 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatcacc
     6961 tgcaggacag gcgcgcaggt accataactt cgtataatgt atgctatacg aagttatggc
     7021 gcgccctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7081 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7141 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7201 agtcagtgtg gaaaatctct agcacccggg cgattaagga aagggctaga tcattcttga
     7261 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     7321 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     7381 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     7441 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7501 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7561 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7621 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7681 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     7741 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7801 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7861 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7921 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7981 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     8041 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     8101 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     8161 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     8221 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     8281 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     8341 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     8401 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8461 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8521 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8581 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8641 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8701 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8761 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8821 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8881 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8941 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9001 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9061 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9121 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9181 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9241 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     9301 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
     9361 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9421 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
     9481 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     9541 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     9601 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9661 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     9721 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     9781 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     9841 taaatatgta ctacaaactt agtagt
//
