LOCUS       sequence-430242-        9428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system 
            (ThermoFisher)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9428)
  AUTHORS   Pardal AJ, Bowman AJ
  TITLE     A specific role for importin-5 and NASP in the import and nuclear 
            hand-off of monomeric H3.
  JOURNAL   Elife. 2022 Sep 6;11:e81755. doi: 10.7554/eLife.81755.
  PUBMED    36066346
REFERENCE   2  (bases 1 to 9428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-09-6"; volume: "11:e81755. doi"; pages: " 
            10.7554/eLife.81755"
COMMENT     Sequence Label: pCL1161
FEATURES             Location/Qualifiers
     source          1..9428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(138..929,930..998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     761..780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(999..1103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1178..1197)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1369..1748
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1749..1952
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1903..1923
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    1954..1972
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1975..1993
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2003..2027
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2038..2047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2509..2518
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2518..2541
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2542..3288
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             join(3295..3297,3298..3300,3301..4011)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3340..3361)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3601..3620)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             4051..4071
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     CDS             4081..4101
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     misc_feature    join(4711..5285,5286..5288,5289..5297)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4878..4895)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5105..5124
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5298..5300,5301..5618)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     complement(6514..6531)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6520..6744
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6877..6896)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    7028..7075
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7083..8105
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    8235..8356
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8272..8291)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8326..8345
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8405..8421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8405..8421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8418..8440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8429..8445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8453..8459,8460..8477,8478..8483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8498..8519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8636..8653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8807..9395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8887..8906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
       61 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
      121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      421 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
      481 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      541 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
      601 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
      661 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
      721 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
      781 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      841 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      901 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      961 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1021 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1081 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     1141 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     1201 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     1261 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     1321 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     1381 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     1441 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1501 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     1561 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1621 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1681 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1741 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     1801 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     1861 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     1921 gtgtacggtg ggaggtctat ataagcagag ctctccctat cagtgataga gatctcccta
     1981 tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg tcagatccgc tagcgccgcc
     2041 gccatggctc gcactaagca aactgctcgg aagtctactg gtggcaaggc gccacgcaaa
     2101 cagttggcca ctaaggcagc ccgcaaaagc gctccggcca ccggcggcgt gaaaaagccc
     2161 caccgctacc ggccgggcac cgtggctctg cgcgagatcc gccgttatca gaagtccact
     2221 gaactgctta ttcgtaaact acctttccag cgcctggtgc gcgagattgc gcaggacttt
     2281 aaaacagacc tgcgtttcca gagctccgct gtgatggctc tgcaggaggc gtgcgaggcc
     2341 tacttggtag ggctatttga ggacactaac ctgtgcgcca tccacgccaa gcgcgtcact
     2401 atcatgccca aggacatcca gctcgcccgc cgcatccgcg gagagagagc tggaggtggc
     2461 tcaggaggtg gctcaggagg tggctcaggt ggaggcgcgc taccggtcgc caccatggac
     2521 tacaaggatg acgacgataa gggaaagtct tacccaactg tgagtgctga ttaccaggac
     2581 gccgttgaga aggcgaagaa gaagctcaga ggcttcatcg ctgagaagag atgcgctcct
     2641 ctaatgctcc gtttggcatt ccactctgct ggaacctttg acaagggcac gaagaccgga
     2701 ggacccttcg gaaccatcaa gcaccctgcc gaactggctc acagcgctaa caacggtctt
     2761 gacatcgctg ttaggctttt ggagccactc aaggcggagt tccctatttt gagctacgcc
     2821 gatttctacc agttggctgg cgttgttgcc gttgaggtca cgggtggacc taaggttcca
     2881 ttccaccctg gaagagagga caagcctgag ccaccaccag agggtcgctt gcccgatccc
     2941 actaagggtt ctgaccattt gagagatgtg tttggcaaag ctatggggct tactgaccaa
     3001 gatatcgttg ctctatctgg gggtcacact attggagctg cacacaagga gcgttctgga
     3061 tttgagggtc cctggacctc taatcctctt attttcgaca actcatactt cacggagttg
     3121 ttgagtggtg agaaggaagg actccttcag ctaccttctg acaaggctct tttgtctgac
     3181 cctgtattcc gccctctcgt tgacaaatat gcagcggacg aagatgcctt ctttgctgat
     3241 tacgctgagg ctcaccaaaa gctctccgag cttgggtttg ctgatgccgg taccatggtg
     3301 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3361 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3421 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3481 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3541 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3601 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3661 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3721 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3781 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3841 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3901 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtccttaag
     3961 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtactcagat
     4021 ctcgagggag gttccggcgg gagcggagga gaaactgtaa gattccaaag cggtggatcc
     4081 gaaaccgtgc gctttcagag cggtggcggg ggaagcggcg gatcaggtgg cgcgggaggt
     4141 ggctctggag gtagcggcgg cgtggcgatt ctgtggcatg aaatgtggca tgaaggcctg
     4201 gaagaagcga gccgcctgta ttttggcgaa cgcaacgtga aaggcatgtt tgaagtgctg
     4261 gaaccgctgc atgcgatgat ggaacgcggc ccgcagaccc tgaaagaaac cagctttaac
     4321 caggcgtatg gccgcgatct gatggaagcg caggaatggt gccgcaaata tatgaaaagc
     4381 ggcaacgtga aagatctgac ccaggcgtgg gatctgtatt atcatgtgtt tcgccgcatt
     4441 ggcggcaccg gcggcagcgg aggtaccggc ggtagcgggg gatcaggtgg cagcggaggg
     4501 accggtggaa gcgagctcaa gcttcgcggc gatggcgaac cgagcggcgt gccggtggcg
     4561 gtggtgctgc tgccggtgtt tgcgctgacc ctggtggcgg tgtgggcgtt tgtgcgctat
     4621 cgcaaacagc tgtagcgcta ccggactcag atctcgagct caagcttcga attctgcagt
     4681 cgacggtacc gcgggcccgg gatcgatccg cccctctccc tccccccccc ctaacgttac
     4741 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     4801 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     4861 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     4921 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     4981 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     5041 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     5101 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     5161 ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt agtcgaggtt
     5221 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     5281 ataatatggc cacaaccatg gtgcaggtgg aaaccattag cccgggcgat ggccgcacct
     5341 ttccgaaacg cggccagacc tgcgtggtgc attataccgg catgctggaa gatggcaaaa
     5401 aatttgatag cagccgcgat cgcaacaaac cgtttaaatt tatgctgggc aaacaggaag
     5461 tgattcgcgg ctgggaagaa ggcgtggcgc agatgagcgt gggccagcgc gcgaaactga
     5521 ccattagccc ggattatgcg tatggcgcga ccggccatcc gggcattatt ccgccgcatg
     5581 cgaccctggt gtttgatgtg gaactgctga aactggaagg cggctccggc ggcagcggtg
     5641 gatcaggcgg gtctggaggt agcggcggtt caaaggcgct acttaaggga gtgcgagatt
     5701 ttaatccaat ctctgcttgc gtatgcctcc ttgagaactc ctcggatggg catagtgaga
     5761 gactgtttgg cattggtttt ggcccatata tcattgccaa ccaacatctt tttagaagga
     5821 acaatggaga gttgactatc aaaaccatgc atggtgagtt caaagtcaag aactcaacac
     5881 aattgcagat gaaaccagtt gagggcagag acataatagt tatcaaaatg gctaaggact
     5941 tcccaccatt ccctcaaaaa ctaaaattca gacagcctac catcaaagat agagtgtgca
     6001 tggtatccac aaattttcag cagaaaagtg tctctagtct agtgtctgag tcatcacaca
     6061 ttgtgcataa agaggacact tcattctggc aacactggat aacaacaaag gatggacaat
     6121 gtggaagtcc gctggtttca atcattgatg gaaatatttt ggggatccac agcctgacgc
     6181 atacgaccaa tggtagcaat tacttcgtgg aatttcctga gaagttcgta gctacatatc
     6241 ttgatgccgc tgatggttgg tgcaagaatt ggaagttcaa tgctgataag atcagttggg
     6301 gttcctttat tctgtgggaa gatgcgccgg aagattttat gagcggcctg gtgccacgcg
     6361 gtgtgggtcg cgaatatgtg cgctttgcgc cgggcagcta gaaacctccc acacctcccc
     6421 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcctcg
     6481 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     6541 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6601 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6661 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6721 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     6781 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6841 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6901 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6961 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7021 gtacctagaa gttcctattc cgaagttcct attctctaga aagtatagga acttccttgg
     7081 ccaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     7141 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     7201 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     7261 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     7321 gggagttcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     7381 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     7441 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     7501 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     7561 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     7621 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     7681 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     7741 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     7801 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     7861 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     7921 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     7981 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     8041 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     8101 aatagcacgt actacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     8161 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     8221 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8281 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8341 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     8401 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8461 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8521 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8581 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8641 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8701 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8761 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8821 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8881 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8941 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     9001 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     9061 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     9121 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     9181 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9241 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9301 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9361 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9421 ggtctgac
//
