LOCUS       sequence-430244-       10185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNAmir backbone for RNA interference, with mCherry reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10185)
  AUTHORS   Wang D, Xiu J, Zhao J, Luo J
  TITLE     miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded 
            multiplex RNAi at a single-cell level.
  JOURNAL   EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. 
            Epub 2022 Feb 24.
  PUBMED    35201651
REFERENCE   2  (bases 1 to 10185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2022-04-5"; volume: "23(4):e53691. doi"; pages: " 
            10.15252/embr.202153691. Epub 2022 Feb 24"
COMMENT     Sequence Label: LAAPCW
FEATURES             Location/Qualifiers
     source          1..10185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    285..419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(309..328)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     363..382
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        467..571
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(572..640,641..1432)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(790..809)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1603..2191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2092..2111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2345..2362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2479..2500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2515..2520,2521..2538,2539..2545)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2553..2569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2558..2580
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2577..2593
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2577..2593
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2722..3355
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     CDS             3545..3556
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3954..3974
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4035..4745)
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4582..4600
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     promoter        complement(4763..5262)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4811..4830)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5204..5227
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    8160..8275
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(8538..8582)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8767..9000
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9497..9622
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(9669..10185,1..117)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
       61 ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttccaact
      121 actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag
      181 gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat agggtatggg
      241 gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca gacatgataa
      301 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
      361 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg
      421 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      481 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      541 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      601 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      661 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      721 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      781 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      841 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      901 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      961 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     1021 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     1081 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     1141 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     1201 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     1261 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     1321 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     1381 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     1441 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     1501 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     1561 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     1621 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     1681 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     1741 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1801 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1861 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1921 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1981 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2041 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2101 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2161 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2221 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2281 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     2341 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     2401 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     2461 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     2521 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     2581 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgacggta aaacgctagc
     2641 aatgtcaagg cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa
     2701 taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg
     2761 atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca
     2821 agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga
     2881 tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat gcaggatctg
     2941 agggctcgcc actccccagt cccgcccagg ccacgcctcc ctggaaagtc cccagcggaa
     3001 agtcccttgt agcaagctcg atatcagcag ttcttgaagt actccggatg cagctctcgg
     3061 gccacgtgat gaaatgctag gcggctgtca aacctccact ctaacacttc tctctccggg
     3121 tcatccatcc catgcaggct cacagggtgt aacaagctgg tgttctctcc tttattggcc
     3181 tcttctacct tatctggctc aactggtact agcttgtagc accatccaaa ggtcagtgga
     3241 tatctgaccc ctggccctgg tgtgtagttc tgctaatcag ggaagtagcc ttgtgtgtgg
     3301 tagatccaca gatcaaggat atcttgtctt cgttgggagt gaattagccc ttccagtccc
     3361 ctcttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     3421 ggtacccctg caggccaggc ggggaggcgg cccaaaggga gatccgactc gtctgagggc
     3481 gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg
     3541 ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa
     3601 cacaggcgag cagccatgga aaggacgtca gcttccccga caacaccacg gaattgtcag
     3661 tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg
     3721 tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc
     3781 cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa
     3841 cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga
     3901 aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg
     3961 taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg taatccagag
     4021 gttgatgttt aaacctactt gtacagctcg tccatgccgc cggtggagtg gcggccctcg
     4081 gcgcgttcgt actgttccac gatggtgtag tcctcgttgt gggaaaggat gtccaacttg
     4141 atgtccacgt tgtaggctcc gggcagctgc acgggcttct tggccttgta ggtggtcttg
     4201 acttcagcgt cgtagtggcc gccgtccttc agcttcagcc tctgcttgat ctcgcccttc
     4261 agggcaccgt cctcggggta catccgctca gtggaagcct cccagcccat tgtacgacac
     4321 tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca gcttcacctt gtagatgaac
     4381 tcgccgtcct gtagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc
     4441 atcacgcgct cccagttgaa gccctcgggg aaggacagct tcaagtagtc ggggatgtcg
     4501 gcggggtgct tcacgtatgc cttggagccg tacatgaact gaggggacag gatgtcccag
     4561 gcgaagggca gggggccacc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag
     4621 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg
     4681 tgcaccttga agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc
     4741 accatggtgg ttaattaaag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg
     4801 cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg
     4861 ccccgcccct gagcccgccc ctgagcccgc cccccggccc cccccctccc cgcctctgag
     4921 cccagaaagc gaaggagcaa agctgctatt ggccgctgcc ccaaaagcct acccgcttcc
     4981 attgctcagc ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc
     5041 acgtcctgca cgacgcgagc tgcggggcgg ggggggactt cctgactagg ggaggagtag
     5101 aaggtggcgc gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg
     5161 aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag
     5221 cgcatgctcc agactgcctt gggaaaagcg cctcccctac ccggtagaat tgaaaaaagt
     5281 gatttaattt ataccatttt aattcagctt tgtaaaaatg tatcaaagag atagcaaggt
     5341 attcagtttt agtaaacaag ataattgctc gaattctagc cccttgaagt ccgaggcagg
     5401 gccctatttt tagcgcagtt aatggaacag gaactaaatt taccccaaaa agattacgtg
     5461 aatcaggata taacgttatt gagggtgaag atcgtgcatt tgaagatgaa acattgaaaa
     5521 atgttgtaaa tccaaatcca gaatttgatc ctgcatgaaa aataccgctt gaatatggta
     5581 ttaaacatga tgcagatatt attattatga atgacccaga tgctgacaga tttggaatgg
     5641 caataaaaca tgatggtcat tttgtaagat tagatggaaa tcaaacagga ccaattttaa
     5701 ttgattgaaa attatcaaat ctaaaacgct taaatagcat tccaaaaaat ccggctctat
     5761 attcaagttt tgtaacaagt gatttgggtg atagaatcgc tcaggaaaaa tatggagtta
     5821 agattgtaaa aactttaact ggattgaaat gaatgggtag agaaattgct aaagaagaag
     5881 ataacggatt aaattttgtt tttgcttatg aagaaagtta tggatatgta attgatgact
     5941 cagctagaga taaagatgga atacaaggtt ctatattgat agcagaggct gcttgatttt
     6001 gtaaaaaaca aaataaaaca ttagtggact atttagaaga tttattgaaa gaaatgggtg
     6061 catattacac tttcacttga aacttgaatt ttgaaccaga agaaaagaaa ttaaaaattg
     6121 aaccattagt gaaatcattg agagcaacac ccttaactca aattgctgga cttaaagttg
     6181 ttaatgtgga tcctgtcaac agcaatatac cttctcgagc cttctgttgg gttaagaagt
     6241 cgaagtaatc ccagcaagtg tttccaagat gtgcaggcaa cgattctgta aagtactgaa
     6301 gcctcattca aacaattacc ctgttatccc tagtcgaagc gctgatctgt aacggcgcag
     6361 aacagaaaac gaaacaaaga cgtagagttg agcaagcagg gtcaggcaaa gcgtggagag
     6421 ccggctgagt ctaggtaggc tccaagggag cgccggacaa aggcccggtc tcgacctgag
     6481 ctctaaactt acctagacgg cggacgtagt tcaggaggca ccacaggcgg gaggcggcag
     6541 aacgcgactc aaccggcgtg gatggcggcc tcaggtaagg cggcgggcgc gtgaaggaga
     6601 gatgcgagcc tggacgggcg gcggatcggc aaaggcgagg ctctgtgctc gcggggcgga
     6661 cgcggtctcg gcggtggtgg cgcgtcgcgc cgctgggttt tatagggcgc cgccgcggcc
     6721 gttcgagcca taaaaggcaa ctttcggaac ggcgcacgct gattggcccc gcgccgctca
     6781 ctcaccggct tcgccgcaca gtgcagcatt tttttacccc ctctcccctc cttttgcgaa
     6841 aaaaaaaaag agcgagagcg agattgagga agaggaggag ggagagtttt ggcgttggcc
     6901 gccttggggt gctgggccgg ggggctgggg gcgcgcgccg tggcccccgc gccccacgct
     6961 gggcagtgcc cggttcggcc ccgcatggcc aggcctgccc ccggcctgcc cgtctctctg
     7021 gccccccacc caccgcggga catcctagtt gtggacatct cttgggcact gagcgcccag
     7081 gtggggtggg ccagggtctg cacgggtgcc aaggccctgg gttctgtacg ctcctgcaga
     7141 aggagctctt ggagggcatg gagtggccag gcagtcactc ccccttgccg acttcagagc
     7201 aactgccctg aaagcagggc ctgaggacct ctggctgtgg ggctcagcta gctaaatgtg
     7261 ctgggtgggt cactagggag agacctgggc ttgagaggta gagtgtggtg ttgggggagt
     7321 caggtggctt gcggccactt agaagtcgca ggaccacact ccccaggaca gggcaggggc
     7381 cagcggtcca gtggctggag gtggcccgtg atgaaggcta caaacctacc cagccgcagc
     7441 cctgggaagg aaggtgggct ctacagggca gggcaccttt taccctggag ctgcctgctt
     7501 ttgagggtaa cagtcacgcc cagccaagac caggcctggg gcgttagtgg gtgacctagt
     7561 cactgcgggg cgggggggct gggtctacac agcctgggtc taggcccacc gtccgttgta
     7621 tgtctgctat gcgcagccac agctgaactg ccctcccaga ccatctggag gccgctgggg
     7681 gactctgggg accaagactc catgtgccac agaggattgg gggcggggcg gtgctaggaa
     7741 ctcaaagcca gcctgggaag accctgtcct tgtcaccctt tcttgccttg ggtctgtcca
     7801 ctgagtagca cacaagaccg ggtgggcagg gtccgttctg ctccgggaat cacagactgt
     7861 gtgtacccag gtggtgggca tgcagcgatc agtggcgtgg gaccacagag gggcattcgg
     7921 ctcagcctag aggaacctgc cttcctcctc ctccccctcc ttggaggtgg tgacaagaag
     7981 ctggattttt acacaaaaat aggaagagtg gtgaggtggg ggcacctcag gcctgcgggg
     8041 ctctttgcag cgcgtagtag gtgttcattg agtgtgtgtc gcaataggca aagatgagtc
     8101 catataagga catggaacat cgataaactg gatctctgct gtccctgtaa taaacccgaa
     8161 aattttgaat ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta
     8221 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaggcga
     8281 taccgtcgag atccgttcac taatcgaatg gatctgtctc tgtctctctc tccaccttct
     8341 tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat
     8401 aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa aactattctt
     8461 aaacctacca agcctcctac tatcattatg aataatttta tataccacag ccaatttgtt
     8521 atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc ttgttcattc
     8581 ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta
     8641 atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag agatttatta
     8701 ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga gcaaccccaa
     8761 atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct tgcctggagc
     8821 tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat agccctcagc
     8881 aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac cgtcagcgtc
     8941 attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa caaagctcct
     9001 attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt gggtgctact
     9061 cctaatggtt caatttttac tactttatat ttatataatt cacttctcca attgtccctc
     9121 atatctcctc ctccaggtct gaagatcagc ggccggccgc ttgctgtgcg gtggtcttac
     9181 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     9241 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     9301 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     9361 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     9421 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     9481 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     9541 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     9601 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     9661 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     9721 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     9781 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     9841 gagagaccca gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact
     9901 ccccagtccc gcccaggcca cgcctccctg gaaagtcccc agcggaaagt cccttgtagc
     9961 aagctcgata tcagcagttc ttgaagtact ccggatgcag ctctcgggcc acgtgatgaa
    10021 atgctaggcg gctgtcaaac ctccactcta acacttctct ctccgggtca tccatcccat
    10081 gcaggctcac agggtgtaac aagctggtgt tctctccttt attggcctct tctaccttat
    10141 ctggctcaac tggtactagc ttgtagcacc atccaaaggt cagtg
//
