LOCUS       sequence-430245-        9486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral dual expression of TAZ4SA and tagBFP with loxP site for 
            Cre excision.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Burgess CL, Huang J, Bawa PS, Alysandratos KD, Minakin K, Ayers LJ, 
            Morley MP, Babu A, Villacorta-Martin C, Yampolskaya M, Hinds A, 
            Thapa BR, Wang F, Matschulat A, Mehta P, Morrisey EE, Varelas X, 
            Kotton DN
  TITLE     Generation of human alveolar epithelial type I cells from 
            pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017.
  PUBMED    38642558
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2024 Apr 15:S1934-5909(24)00098-5. doi: 
            10.1016/j.stem.2024.03.017."
COMMENT     Sequence Label: p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP
FEATURES             Location/Qualifiers
     source          1..9486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3406..3415
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3415..3480
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    3484..3508
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4712..4736)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4747..5145
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             5160..5861
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    5880..6468
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5933..5953)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6351..6362)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    6604..6637
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6664..6844
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6891..6909)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6977..7081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7082..7150,7151..7942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7300..7319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8113..8701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8855..8872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9112..9133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9148..9153,9154..9171,9172..9178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9186..9202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9191..9213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9210..9226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9210..9226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9276..9295
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc catggactac
     3421 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
     3481 cttacaagtt tgtacaaaaa agttggcatg aatccggcct cggcgccccc tccgctcccg
     3541 ccgcctgggc agcaagtgat ccacgtcacg caggacctag acacagacct cgaagccctc
     3601 ttcaactctg tcatgaatcc gaagcctagc tcgtggcgga agaagatcct gccggagtct
     3661 ttctttaagg agcctgattc gggctcgcac tcgcgccagt ccgccaccga ctcgtccgga
     3721 ggccacccgg ggcctcgact ggctgggggt gcccagcatg tccgctcgca cgcctcgccc
     3781 gcgtccctgc agctgggcac cggcgcgggt gctgcgggta gccccgcgca gcagcacgcg
     3841 cacctccgcc agcaggccta cgacgtgacc gacgagctgc cactgccccc gggctgggag
     3901 atgaccttca cggccactgg ccagaggtac ttcctcaatc acatagaaaa aatcaccaca
     3961 tggcaagacc ctaggaaggc gatgaatcag cctctgaatc atatgaacct ccaccctgcc
     4021 gtcagttcca caccagtgcc tcagaggtcc atggcagtat cccagccaaa tctcgtgatg
     4081 aatcaccaac accagcagca gatggccccc agtaccctga gccagcagaa ccaccccact
     4141 cagaacccac ccgcagggct catgagtatg cccaatgcgc tgaccactca gcagcagcag
     4201 cagcagaaac tgcggcttca gagaatccag atggagagag aaaggattcg aatgcgccaa
     4261 gaggagctca tgaggcagga agctgccctc tgtcgacagc tccccatgga agctgagact
     4321 cttgccccag ttcaggctgc tgtcaaccca cccacgatga ccccagacat gagatccatc
     4381 actaataata gctcagatcc tttcctcaat ggagggccat atcattcgag ggagcaggcc
     4441 actgacagtg gcctggggtt agggtgctac agtgtcccca caactccgga ggacttcctc
     4501 agcaatgtgg atgagatgga tacaggagaa aacgcaggac aaacacccat gaacatcaat
     4561 ccccaacaga cccgtttccc tgatttcctt gactgtcttc caggaacaaa cgttgactta
     4621 ggaactttgg aatctgaaga cctgatcccc ctcttcaatg atgtagagtc tgctctgaac
     4681 aaaagtgagc cctttctaac ctggctgtaa tacccaactt tcttgtacaa agtggtgtga
     4741 ggatccggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga
     4801 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca
     4861 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca
     4921 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag
     4981 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac
     5041 gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat
     5101 ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggccgcc catatggcca
     5161 tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca
     5221 accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
     5281 tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta
     5341 gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca
     5401 agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg
     5461 tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga
     5521 tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg
     5581 aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg
     5641 ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca
     5701 agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa
     5761 gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat
     5821 actgcgacct ccctagcaaa ctggggcaca agcttaatta aaggatatcg atagatccta
     5881 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5941 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6001 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6061 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6121 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6181 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6241 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6301 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6361 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6421 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta
     6481 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6541 ctggaagggc taattcactc ccaacgaaga caagatcacc tgcaggacag gcgcgcaggt
     6601 accataactt cgtataatgt atgctatacg aagttatggc gcgccctgct ttttgcttgt
     6661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6721 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6781 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6841 agcacccggg cgattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     6901 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     6961 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7021 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7081 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7141 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7201 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7261 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7321 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7381 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7441 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7501 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7561 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7621 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7681 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7741 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7801 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7861 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7921 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7981 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8041 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8101 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8161 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8221 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8281 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8341 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8401 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8461 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8521 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8581 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8641 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8701 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8761 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8821 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8881 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
     8941 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9001 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
     9061 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     9121 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     9181 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
     9241 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     9301 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     9361 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     9421 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     9481 agtagt
//
