LOCUS       sequence-430249-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of wild type TEX264, C-terminal Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, 
            Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, 
            Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, 
            Ramadan K
  TITLE     TEX264 coordinates p97- and SPRTN-mediated resolution of 
            topoisomerase 1-DNA adducts.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
  PUBMED    32152270
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1274. doi"; pages: " 
            10.1038/s41467-020-15000-w"
COMMENT     Sequence Label: pCDNA3-TEX264wt-cFlag
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..56
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     101..120
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        101..119
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1096..1119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccnntnnngn nntgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct
       61 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
      121 agacccaagc ttggtaccga gctcggatcc atgtcggacc tgctactact gggcctgatt
      181 gggggcctga ctctcttact gctgctgacg ctgctggcct ttgccgggta ctcagggcta
      241 ctggctgggg tggaagtgag tgctgggtca ccccccatcc gcaacgtcac tgtggcctac
      301 aagttccaca tggggctcta tggtgagact gggcggcttt tcactgagag ctgcagcatc
      361 tctcccaagc tccgctccat cgctgtctac tatgacaacc cccacatggt gccccctgat
      421 aagtgccgat gtgccgtggg cagcatcctg agtgaaggtg aggaatcgcc ctcccctgag
      481 ctcatcgacc tctaccagaa atttggcttc aaggtgttct ccttcccggc acccagccat
      541 gtggtgacag ccaccttccc ctacaccacc attctgtcca tctggctggc tacccgccgt
      601 gtccatcctg ccttggacac ctacatcaag gagcggaagc tgtgtgccta tcctcggctg
      661 gagatctacc aggaagacca gatccatttc atgtgcccac tggcacggca gggagacttc
      721 tatgtgcctg agatgaagga gacagagtgg aaatggcggg ggcttgtgga ggccattgac
      781 acccaggtgg atggcacagg agctgacaca atgagtgaca cgagttctgt aagcttggaa
      841 gtgagccctg gcagccggga gacttcagct gccacactgt cacctggggc gagcagccgt
      901 ggctgggatg acggtgacac ccgcagcgag cacagctaca gcgagtcagg tgccagcggc
      961 tcctcttttg aggagctgga cttggagggc gaggggccct taggggagtc acggctggac
     1021 cctgggactg agcccctggg gactaccaag tggctctggg agcccactgc ccctgagaag
     1081 ggcaaggaga gcgctgacta caaagacgat gacgacaagt aagcggccgc tcgagcatgc
     1141 atctagaggg ccctattcta tagntcncnn naa
//
