LOCUS       sequence-430251-        1176 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of TEX264 with SIM1 motif mutated, C-terminal 
            Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, 
            Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, 
            Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, 
            Ramadan K
  TITLE     TEX264 coordinates p97- and SPRTN-mediated resolution of 
            topoisomerase 1-DNA adducts.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
  PUBMED    32152270
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1274. doi"; pages: " 
            10.1038/s41467-020-15000-w"
COMMENT     Sequence Label: pCDNA3-TEX264-SIM1_mut-cFlag
FEATURES             Location/Qualifiers
     source          1..1176
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     90..109
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        90..108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1085..1108
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gccggttggg taaccgggtg gagagggtct atataagcag agctctctgg ctctctagaa
       61 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
      121 tggtaccgag ctcggatcca tgtcggacct gctactactg ggcctgattg ggggcctgac
      181 tctcttactg ctgctgacgc tgctggcctt tgccgggtac tcagggctac tggctggggt
      241 ggaagtgagt gctgggtcac cccccatccg caacgtcact gtggcctaca agttccacat
      301 ggggctctat ggtgagactg ggcggctttt cactgagagc tgcagcatct ctcccaagct
      361 ccgctccatc gctgtctact atgacaaccc ccacatggtg ccccctgata agtgccgatg
      421 tgccgtgggc agcatcctga gtgaaggtga ggaatcgccc tcccctgagg ccgccgccgc
      481 cgcccagaaa tttggcttca aggtgttctc cttcccggca cccagccatg tggtgacagc
      541 caccttcccc tacaccacca ttctgtccat ctggctggct acccgccgtg tccatcctgc
      601 cttggacacc tacatcaagg agcggaagct gtgtgcctat cctcggctgg agatctacca
      661 ggaagaccag atccatttca tgtgcccact ggcacggcag ggagacttct atgtgcctga
      721 gatgaaggag acagagtgga aatggcgggg gcttgtggag gccattgaca cccaggtgga
      781 tggcacagga gctgacacaa tgagtgacac gagttctgta agcttggaag tgagccctgg
      841 cagccgggag acttcagctg ccacactgtc acctggggcg agcagccgtg gctgggatga
      901 cggtgacacc cgcagcgagc acagctacag cgagtcaggt gccagcggct cctcttttga
      961 ggagctggac ttggagggcg aggggccctt aggggagtca cggctggacc ctgggactga
     1021 gcccctgggg actaccaagt ggctctggga gcccactgcc cctgagaagg gcaaggagag
     1081 cgctgactac aaagacgatg acgacaagta agcggccgct cgagcatgca ttctagaggg
     1141 ccctattcta tagtttcaac ctaaaaacaa gcccca
//
