LOCUS       sequence-430255-        1190 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of TEX264 with E194A mutation, C-terminal Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, 
            Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, 
            Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, 
            Ramadan K
  TITLE     TEX264 coordinates p97- and SPRTN-mediated resolution of 
            topoisomerase 1-DNA adducts.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
  PUBMED    32152270
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1274. doi"; pages: " 
            10.1038/s41467-020-15000-w"
COMMENT     Sequence Label: pCDNA3-TEX264-E194A-cFlag
FEATURES             Location/Qualifiers
     source          1..1190
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..63
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     108..127
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        108..126
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1103..1126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accccccttt ggacgcaatg gggcggtagg cgtgtacggt gggaggtcta tataagcaga
       61 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
      121 tatagggaga cccaagcttg gtaccgagct cggatccatg tcggacctgc tactactggg
      181 cctgattggg ggcctgactc tcttactgct gctgacgctg ctggcctttg ccgggtactc
      241 agggctactg gctggggtgg aagtgagtgc tgggtcaccc cccatccgca acgtcactgt
      301 ggcctacaag ttccacatgg ggctctatgg tgagactggg cggcttttca ctgagagctg
      361 cagcatctct cccaagctcc gctccatcgc tgtctactat gacaaccccc acatggtgcc
      421 ccctgataag tgccgatgtg ccgtgggcag catcctgagt gaaggtgagg aatcgccctc
      481 ccctgagctc atcgacctct accagaaatt tggcttcaag gtgttctcct tcccggcacc
      541 cagccatgtg gtgacagcca ccttccccta caccaccatt ctgtccatct ggctggctac
      601 ccgccgtgtc catcctgcct tggacaccta catcaaggag cggaagctgt gtgcctatcc
      661 tcggctggag atctaccagg aagaccagat ccatttcatg tgcccactgg cacggcaggg
      721 agacttctat gtgcctgcga tgaaggagac agagtggaaa tggcgggggc ttgtggaggc
      781 cattgacacc caggtggatg gcacaggagc tgacacaatg agtgacacga gttctgtaag
      841 cttggaagtg agccctggca gccgggagac ttcagctgcc acactgtcac ctggggcgag
      901 cagccgtggc tgggatgacg gtgacacccg cagcgagcac agctacagcg agtcaggtgc
      961 cagcggctcc tcttttgagg agctggactt ggagggcgag gggcccttag gggagtcacg
     1021 gctggaccct gggactgagc ccctggggac taccaagtgg ctctgggagc ccactgcccc
     1081 tgagaagggc aaggagagcg ctgactacaa agacgatgac gacaagtaag cggccgctcg
     1141 agcatgcatc tagagggccc tattctatag ttcacctaaa agacaccccc
//
