LOCUS       sequence-430257-        1197 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of TEX264 with SHP domain mutated, C-terminal 
            Flag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, 
            Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, 
            Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, 
            Ramadan K
  TITLE     TEX264 coordinates p97- and SPRTN-mediated resolution of 
            topoisomerase 1-DNA adducts.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
  PUBMED    32152270
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1274. doi"; pages: " 
            10.1038/s41467-020-15000-w"
COMMENT     Sequence Label: pCDNA3-TEX264-SHP1mut-cFlag
FEATURES             Location/Qualifiers
     source          1..1197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        15..65
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     110..129
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        110..128
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1105..1128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgcccccaca tttggacgga aatgggcggt ggcgtgtacg gtgggaggtc tatataagca
       61 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      121 actataggga gacccaagct tggtaccgag ctcggatcca tgtcggacct gctactactg
      181 ggcctgattg ggggcctgac tctcttactg ctgctgacgc tgctggcctt tgccgggtac
      241 tcagggctac tggctggggt ggaagtgagt gctgggtcac cccccatccg caacgtcact
      301 gtggcctaca agttccacat ggggctctat ggtgagactg ggcggctttt cactgagagc
      361 tgcagcatct ctcccaagct ccgctccatc gctgtctact atgacaaccc ccacatggtg
      421 ccccctgata agtgccgatg tgccgtgggc agcatcctga gtgaaggtga ggaatcgccc
      481 tcccctgagc tcatcgacct ctaccagaaa tttggcttca aggtgttctc cttcccggca
      541 cccagccatg tggtgacagc caccttcccc tacaccacca ttctgtccat ctggctggct
      601 acccgccgtg tccatcctgc cttggacacc tacatcaagg agcggaagct gtgtgcctat
      661 cctcggctgg agatctacca ggaagaccag atccatttca tgtgcccact ggcacggcag
      721 ggagacttct atgtgcctga gatgaaggag acagagtgga aatggcgggg gcttgtggag
      781 gccattgaca cccaggtgga tggcacagga gctgacacaa tgagtgacac gagttctgta
      841 agcttggaag tgagccctgg cagccgggag acttcagctg ccacactgtc acctggggcg
      901 agcagccgtg gctgggatga cggtgacacc cgcagcgagc acagctacag cgagtcaggt
      961 gccagcggct cctcttttga ggagctggac ttggagcgcg agaggcccgc aggggagtca
     1021 cggctggacc ctgggactga gcccctgggg actaccaagt ggctctggga gcccactgcc
     1081 cctgagaagg gcaaggagag cgctgactac aaagacgatg acgacaagta agcggccgct
     1141 cgagcatgca tctagagggc cctattctat agtgtcaacc taaaaagcaa aaaaccc
//
