LOCUS       sequence-430259-        1231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, 
            Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, 
            Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, 
            Ramadan K
  TITLE     TEX264 coordinates p97- and SPRTN-mediated resolution of 
            topoisomerase 1-DNA adducts.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
  PUBMED    32152270
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1274. doi"; pages: " 
            10.1038/s41467-020-15000-w"
COMMENT     Sequence Label: pCDNA5 TEX264wt-cSSH
FEATURES             Location/Qualifiers
     source          1..1231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1071..1094
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1131..1154
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1188..1214
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgagccctcc cgctgtttgg gcctccagag tgagacaccg ggaccgatcg agcctccgga
       61 ctctagcgtt taaacttaag cttggtaccg agctcggatc catgtcggac ctgctactac
      121 tgggcctgat tgggggcctg actctcttac tgctgctgac gctgctggcc tttgccgggt
      181 actcagggct actggctggg gtggaagtga gtgctgggtc accccccatc cgcaacgtca
      241 ctgtggccta caagttccac atggggctct atggtgagac tgggcggctt ttcactgaga
      301 gctgcagcat ctctcccaag ctccgctcca tcgctgtcta ctatgacaac ccccacatgg
      361 tgccccctga taagtgccga tgtgccgtgg gcagcatcct gagtgaaggt gaggaatcgc
      421 cctcccctga gctcatcgac ctctaccaga aatttggctt caaggtgttc tccttcccgg
      481 cacccagcca tgtggtgaca gccaccttcc cctacaccac cattctgtcc atctggctgg
      541 ctacccgccg tgtccatcct gccttggaca cctacatcaa ggagcggaag ctgtgtgcct
      601 atcctcggct ggagatctac caggaagacc agatccattt catgtgccca ctggcacggc
      661 agggagactt ctatgtgcct gagatgaagg agacagagtg gaaatggcgg gggcttgtgg
      721 aggccattga cacccaggtg gatggcacag gagctgacac aatgagtgac acgagttctg
      781 taagcttgga agtgagccct ggcagccggg agacttcagc tgccacactg tcacctgggg
      841 cgagcagccg tggctgggat gacggtgaca cccgcagcga gcacagctac agcgagtcag
      901 gtgccagcgg ctcctctttt gaggagctgg acttggaggg cgaggggccc ttaggggagt
      961 cacggctgga ccctgggact gagcccctgg ggactaccaa gtggctctgg gagcccactg
     1021 cccctgagaa gggcaaggag gcggccgctc gagggaacct tttagcagag tggagccacc
     1081 cgcagttcga gaaaggtgga ggttccggag gtggatcggg aggtggatcg tggagccacc
     1141 cgcagtttga aaaaggaacc gaactaggtt ccacgatggc aagcatgtac ccatacgatg
     1201 ttcctgacta tgcctaggtc gccgtacttg c
//
