LOCUS       sequence-430263-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of wild type TEX264 fused at C-terminus to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, 
            Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, 
            Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, 
            Ramadan K
  TITLE     TEX264 coordinates p97- and SPRTN-mediated resolution of 
            topoisomerase 1-DNA adducts.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
  PUBMED    32152270
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1274. doi"; pages: " 
            10.1038/s41467-020-15000-w"
COMMENT     Sequence Label: pCDNA5 TEX264wt-cGFP
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..60
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     protein_bind    62..80
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     111..135
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 accccattga cccaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
       61 ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg agctcgttta
      121 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
      181 cgggaccgat ccagcctccg gactctagcg tttaaactta agcttggtac cgagctcgga
      241 tccatgtcgg acctgctact actgggcctg attgggggcc tgactctctt actgctgctg
      301 acgctgctgg cctttgccgg gtactcaggg ctactggctg gggtggaagt gagtgctggg
      361 tcacccccca tccgcaacgt cactgtggcc tacaagttcc acatggggct ctatggtgag
      421 actgggcggc ttttcactga gagctgcagc atctctccca agctccgctc catcgctgtc
      481 tactatgaca acccccacat ggtgccccct gataagtgcc gatgtgccgt gggcagcatc
      541 ctgagtgaag gtgaggaatc gccctcccct gagctcatcg acctctacca gaaatttggc
      601 ttcaaggtgt tctccttccc ggcacccagc catgtggtga cagccacctt cccctacacc
      661 accattctgt ccatctggct ggctacccgc cgtgtccatc ctgccttgga cacctacatc
      721 aaggagcgga agctgtgtgc ctatcctcgg ctggagatct accaggaaga ccagatccat
      781 ttcatgtgcc cactggcacg gcagggagac ttctatgtgc ctgagatgaa ggagacagag
      841 tggaaatggc gggggcttgt ggaggccatt gacacccagg tggatggcac aggagctgac
      901 acaatgagtg acacgagttc tgtaagcttg gaagtgagcc ctggcagccg ggagacttca
      961 gctgccacac tgtcacctgg ggcgagcagc cgtggctggg atgacggtga cacccgcagc
     1021 gagcacagct acagcgagtc aggtgccagc ggctcctctt ttgaggagct ggacttggag
     1081 ggcgaggggc ccttagggga gtcacggctg gaccctggga ctgagcccct ggggactacc
     1141 aagtggctct gggagcccac tgcccctgag aagggcaagg agctcgaggg tggaggttcc
     1201 ggaggtggat cgggtggaat ggtgagcaa
//
