LOCUS       sequence-430265-        1222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of TEX264 with SIM1 motif mutated, fused at 
            C-terminus to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, 
            Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, 
            Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, 
            Ramadan K
  TITLE     TEX264 coordinates p97- and SPRTN-mediated resolution of 
            topoisomerase 1-DNA adducts.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
  PUBMED    32152270
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1274. doi"; pages: " 
            10.1038/s41467-020-15000-w"
COMMENT     Sequence Label: pCDNA5-TEX264-SIM1_mut-cGFP
FEATURES             Location/Qualifiers
     source          1..1222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..55
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     protein_bind    57..75
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    78..96
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     106..130
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tgaacgcaaa ttgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctccc
       61 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
      121 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      181 ccgatccagc ctccggactc tagcgtttaa acttaagctt ggtaccgagc tcggatccat
      241 gtcggacctg ctactactgg gcctgattgg gggcctgact ctcttactgc tgctgacgct
      301 gctggccttt gccgggtact cagggctact ggctggggtg gaagtgagtg ctgggtcacc
      361 ccccatccgc aacgtcactg tggcctacaa gttccacatg gggctctatg gtgagactgg
      421 gcggcttttc actgagagct gcagcatctc tcccaagctc cgctccatcg ctgtctacta
      481 tgacaacccc cacatggtgc cccctgataa gtgccgatgt gccgtgggca gcatcctgag
      541 tgaaggtgag gaatcgccct cccctgaggc cgccgccgcc gcccagaaat ttggcttcaa
      601 ggtgttctcc ttcccggcac ccagccatgt ggtgacagcc accttcccct acaccaccat
      661 tctgtccatc tggctggcta cccgccgtgt ccatcctgcc ttggacacct acatcaagga
      721 gcggaagctg tgtgcctatc ctcggctgga gatctaccag gaagaccaga tccatttcat
      781 gtgcccactg gcacggcagg gagacttcta tgtgcctgag atgaaggaga cagagtggaa
      841 atggcggggg cttgtggagg ccattgacac ccaggtggat ggcacaggag ctgacacaat
      901 gagtgacacg agttctgtaa gcttggaagt gagccctggc agccgggaga cttcagctgc
      961 cacactgtca cctggggcga gcagccgtgg ctgggatgac ggtgacaccc gcagcgagca
     1021 cagctacagc gagtcaggtg ccagcggctc ctcttttgag gagctggact tggagggcga
     1081 ggggccctta ggggagtcac ggctggaccc tgggactgag cccctgggga ctaccaagtg
     1141 gctctgggag cccactgccc ctgagaaggg caaggagctc gagggtggag gttccggagg
     1201 tggattcggg tggaaatggt ga
//
