LOCUS       sequence-430269-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of TEX264, 2-25 residues (LRR) truncated, 
            C-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, 
            Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, 
            Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, 
            Ramadan K
  TITLE     TEX264 coordinates p97- and SPRTN-mediated resolution of 
            topoisomerase 1-DNA adducts.
  JOURNAL   Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
  PUBMED    32152270
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-03-9"; volume: "11(1):1274. doi"; pages: " 
            10.1038/s41467-020-15000-w"
COMMENT     Sequence Label: pET21a-TEX264-deltaLRR-His6
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..30
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             920..937
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aaataatttt gtttaacttt aagaaggaga tatacatatg gggtactcag ggctactggc
       61 tggggtggaa gtgagtgctg ggtcaccccc catccgcaac gtcactgtgg cctacaagtt
      121 ccacatgggg ctctatggtg agactgggcg gcttttcact gagagctgca gcatctctcc
      181 caagctccgc tccatcgctg tctactatga caacccccac atggtgcccc ctgataagtg
      241 ccgatgtgcc gtgggcagca tcctgagtga aggtgaggaa tcgccctccc ctgagctcat
      301 cgacctctac cagaaatttg gcttcaaggt gttctccttc ccggcaccca gccatgtggt
      361 gacagccacc ttcccctaca ccaccattct gtccatctgg ctggctaccc gccgtgtcca
      421 tcctgccttg gacacctaca tcaaggagcg gaagctgtgt gcctatcctc ggctggagat
      481 ctaccaggaa gaccagatcc atttcatgtg cccactggca cggcagggag acttctatgt
      541 gcctgagatg aaggagacag agtggaaatg gcgggggctt gtggaggcca ttgacaccca
      601 ggtggatggc acaggagctg acacaatgag tgacacgagt tctgtaagct tggaagtgag
      661 ccctggcagc cgggagactt cagctgccac actgtcacct ggggcgagca gccgtggctg
      721 ggatgacggt gacacccgca gcgagcacag ctacagcgag tcaggtgcca gcggctcctc
      781 ttttgaggag ctggacttgg agggcgaggg gcccttaggg gagtcacggc tggaccctgg
      841 gactgagccc ctggggacta ccaagtggct ctgggagccc actgcccctg agaagggcaa
      901 ggaggcggcc gcactcgagc accaccacca ccaccactga gatccggctg ctaacaaag
//
