LOCUS       sequence-430271-        7065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRC1 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..7065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    280..401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(291..310)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     345..364
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    505..899
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(913..1287)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        1880..2183
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2263..2851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2343..2362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2959..3007
                     /note="synthetic polyadenylation signal"
     misc_feature    3018..3109
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3058..3077
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3146..3357
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3370..3638
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     3688..3747
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             3748..3774
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6607..6630
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt ggagtgggag
       61 agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga ctccgacggc
      121 tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca ggggaacgtc
      181 ttctcatgct ccgtgatgca cgaggctctg cacaaccact acacgcagaa gagcctctcc
      241 ctgtctccgg gtaaatgagt gctagctggc cagacatgat aagatacatt gatgagtttg
      301 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
      361 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
      421 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
      481 acaaatgtgg tatggaatta attctaaaat acagcatagc aaaactttaa cctccaaatc
      541 aagcctctac ttgaatcctt ttctgaggga tgaataaggc ataggcatca ggggctgttg
      601 ccaatgtgca ttagctgttt gcagcctcac cttctttcat ggagtttaag atatagtgta
      661 ttttcccaag gtttgaacta gctcttcatt tctttatgtt ttaaatgcac tgacctccca
      721 cattcccttt ttagtaaaat attcagaaat aatttaaata catcattgca atgaaaataa
      781 atgtttttta ttaggcagaa tccagatgct caaggccctt cataatatcc cccagtttag
      841 tagttggact tagggaacaa aggaaccttt aatagaaatt ggacagcaag aaagcgagct
      901 tctagcttat cctcagtcct gctcctctgc cacaaagtgc acgcagttgc cggccgggtc
      961 gcgcagggcg aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga
     1021 ggcgtcccgg aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc
     1081 gcgcacccac acccaggcca gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat
     1141 gaacagggtc acgtcgtccc ggaccacacc ggcgaagtcg tcctccacga agtcccggga
     1201 gaacccgagc cggtcggtcc agaactcgac cgctccggcg acgtcgcgcg cggtgagcac
     1261 cggaacggca ctggtcaact tggccatgat ggctcctcct gtcaggagag gaaagagaag
     1321 aaggttagta caattgctat agtgagttgt attatactat gcagatatac tatgccaatg
     1381 attaattgtc aaactagggc tgcagggttc atagtgccac ttttcctgca ctgccccatc
     1441 tcctgcccac cctttcccag gcatagacag tcagtgactt accaaactca caggagggag
     1501 aaggcagaag cttgagacag acccgcggga ccgccgaact gcgaggggac gtggctaggg
     1561 cggcttcttt tatggtgcgc cggccctcgg aggcagggcg ctcggggagg cctagcggcc
     1621 aatctgcggt ggcaggaggc ggggccgaag gccgtgcctg accaatccgg agcacatagg
     1681 agtctcagcc ccccgcccca aagcaagggg aagtcacgcg cctgtagcgc cagcgtgttg
     1741 tgaaatgggg gcttgggggg gttggggccc tgactagtca aaacaaactc ccattgacgt
     1801 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     1861 actgccaaaa ccgcatcatc atggtaatag cgatgactaa tacgtagatg tactgccaag
     1921 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     1981 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     2041 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     2101 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     2161 catttaccgt aagttatgta acgcctgcag gttaattaag aacatgtgag caaaaggcca
     2221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2701 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatggcta gttaattaac
     2941 atttaaatca gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     3001 ttgtgtgaat cgtaactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     3061 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgaaggatc
     3121 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     3181 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     3241 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     3301 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     3361 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     3421 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     3481 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     3541 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     3601 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacc
     3661 tgagatcacc ggcgaaggag ggccaccatg tacaggatgc aactcctgtc ttgcattgca
     3721 ctaagtcttg cacttgtcac gaattcgtac ccatacgatg ttccagatta cgctggatcc
     3781 aaaattgaag aaggcaaact ggtgatttgg attaacggcg ataaaggcta taacggcctg
     3841 gcggaagtgg gcaaaaaatt tgaaaaagat accggcatta aagtgaccgt ggaacatccg
     3901 gataaactgg aagaaaaatt tccgcaggtg gcggcgaccg gcgatggccc ggatattatt
     3961 ttttgggcgc atgatcgctt tggcggctat gcgcagagcg gcctgctggc ggaaattacc
     4021 ccggataaag cgtttcagga taaactgtat ccgtttacct gggatgcggt gcgctataac
     4081 ggcaaactga ttgcgtatcc gattgcggtg gaagcgctga gcctgattta taacaaagat
     4141 ctgctgccga acccgccgaa aacctgggaa gaaattccgg cgctggataa agaactgaaa
     4201 gcgaaaggca aaagcgcgct gatgtttaac ctgcaggaac cgtattttac ctggccgctg
     4261 attgcggcgg atggcggcta tgcgtttaaa tatgaaaacg gcaaatatga tattaaagat
     4321 gtgggcgtgg ataacgcggg cgcgaaagcg ggcctgacct ttctggtgga tctgattaaa
     4381 aacaaacata tgaacgcgga taccgattat agcattgcgg aagcggcgtt taacaaaggc
     4441 gaaaccgcga tgaccattaa cggcccgtgg gcgtggagca acattgatac cagcaaagtg
     4501 aactatggcg tgaccgtgct gccgaccttt aaaggccagc cgagcaaacc gtttgtgggc
     4561 gtgctgagcg cgggcattaa cgcggcgagc ccgaacaaag aactggcgaa agaatttctg
     4621 gaaaactatc tgctgaccga tgaaggcctg gaagcggtga acaaagataa accgctgggc
     4681 gcggtggcgc tgaaaagcta tgaagaagaa ctggcgaaag atccgcgcat tgcggcgacc
     4741 atggaaaacg cgcagaaagg cgaaattatg ccgaacattc cgcagatgag cgcgttttgg
     4801 tatgcggtgc gcaccgcggt gattaacgcg gcgagcggcc gccagaccgt ggatgaagcg
     4861 ctgaaagatg cgcagaccaa cgagaacctt tacttccaaa ctgccagctt cgccgtgctg
     4921 atggacatca gccgtagaga gaacggagaa gtgctgcctc tgaaaatcgt gacatacgcc
     4981 gccgtgtccc tctccctggc tgccctgctg gtggctttcg tgctgctgtc tctggtgcgg
     5041 atgcttcgga gcaacctgca tagcatccac aagcacctgg ccgtggccct gttcctgagc
     5101 cagctggtct ttgtgattgg catcaaccag acagagaatc ctttcctgtg tacagtggtg
     5161 gccatcctgc tccactatat ctacatgagc acattcgcct ggactctggt ggaaagcctg
     5221 cacgtgtaca gaatgctgac agaggtgcgc aacattgaca ccggccctat gcggttctac
     5281 tacgtggtcg gatggggcat ccccgctatc gtgaccggcc tggccgtggg cttggatccc
     5341 cagggctacg gcaaccccga cttctgctgg ctgagcttgc aggacaccct gatctggtct
     5401 ttcgccggcc ccatcggcgc cgtaatcatc atcaacaccg ttacatctgt gctgtctgct
     5461 aaggtgtcct gtcagagaaa gcaccactac tacggaaaaa agggcatcgt gagcctgctg
     5521 cgaaccgcct tcctgctact gctcctgatc agcgccacat ggctgctggg cctgctggcc
     5581 gtgaacagag atgccctgag ctttcactac ctgtttgcca tcttcagcgg cctgcagggc
     5641 cccttcgtgc tgctgttcca ctgcgtgctg aaccaggagg tgagaaagca cctgaagggc
     5701 gtgctgggag gcaggaagct gcacctggaa gattccgcca ccacaagagc caccctgctg
     5761 accagaagcc tgaactgcaa caccaccttc ggcgacggac ctgacatgct gagaacagat
     5821 ctgggcgagt ctaccgccag cctggactcc atcgtgcggg acgagggcat ccagaaactg
     5881 ggagtttcct ccggcctggt gcggggatcc cacggcgaac ccgacgccag cctcatgcct
     5941 cggagctgca aggacccacc aggccacgac agcgattccg acagcgagct gagcctggac
     6001 gagcagagca gcagctacgc ctctagccac agctctgata gcgaggacga cggcgtgggc
     6061 gccgaggaaa agtgggaccc cgccagaggc gccgtgcaca gcacccctaa gggcgatgcc
     6121 gttgctaatc acgtgccagc tggctggcct gatcaatctc tggctgagag cgacagcgag
     6181 gaccccagcg gcaagcctag actgaaggtg gaaaccaagg tgtctgtgga actgcacaga
     6241 gaggaacaag ggagccatag aggcgagtac cctcctgatc aggagagcgg cggcgcggct
     6301 agactggcct ccagccagcc tcctgagcag cggaaaggca tcctgaagaa caaggtcaca
     6361 tatcctcctc cactgaccct gaccgagcag accctgaagg gaagactgag agaaaagctg
     6421 gctgattgcg agcaaagtcc taccagctct agaaccagct ccctgggaag cggaggtcct
     6481 gattgcgcca tcacagtgaa gagccccggc agagaacctg gccgggacca cctaaatggc
     6541 gtcgccatga acgtgcggac cggctctgcc caggccgacg gcagcgactc tgaaaaacct
     6601 ggttctgact acaaagacga tgacgacaag tgaagatctg acaaaactca cacatgccca
     6661 ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc
     6721 aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc
     6781 cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc
     6841 aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc
     6901 gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc
     6961 ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag
     7021 gtgtacaccc tgcccccatc ccgggaggag atgaccaaga accag
//
