LOCUS       sequence-430278-        6345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRD2 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..6345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    282..330
                     /note="synthetic polyadenylation signal"
     misc_feature    341..432
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     381..400
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        469..680
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             693..961
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     1011..1070
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             1071..1097
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3210..3233
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3948..4069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3959..3978)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4013..4032
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    4173..4567
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(4581..4955)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        5548..5851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(join(5931..6345,1..174))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6011..6030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
       61 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      121 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      181 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      241 ttggtcatgg ctagttaatt aacatttaaa tcagcggccg caataaaata tctttatttt
      301 cattacatct gtgtgttggt tttttgtgtg aatcgtaact aacatacgct ctccatcaaa
      361 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca
      421 gaacatttct ctatcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
      481 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
      541 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      601 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      661 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
      721 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
      781 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
      841 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
      901 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
      961 ccaagctgtg accggcgcct acctgagatc accggcgaag gagggccacc atgtacagga
     1021 tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg tacccatacg
     1081 atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt tggattaacg
     1141 gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa gataccggca
     1201 ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag gtggcggcga
     1261 ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc tatgcgcaga
     1321 gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg tatccgttta
     1381 cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg gtggaagcgc
     1441 tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg gaagaaattc
     1501 cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt aacctgcagg
     1561 aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt aaatatgaaa
     1621 acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa gcgggcctga
     1681 cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat tatagcattg
     1741 cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg tgggcgtgga
     1801 gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc tttaaaggcc
     1861 agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg agcccgaaca
     1921 aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc ctggaagcgg
     1981 tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa gaactggcga
     2041 aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt atgccgaaca
     2101 ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac gcggcgagcg
     2161 gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac ctttacttcc
     2221 aaaccagttt cgcaatcctg ctccaaatct atgaggtcca acgcggccct gaggaagagt
     2281 ctcttctgcg cactttgtct tttgtggggt gcggcgtcag tttctgtgcg ctgaccacta
     2341 cctttctcct gttccttgtg gccggagtac ctaagtctga acgcacgacc gtgcacaaga
     2401 acctgacctt ttccttggct tctgccgagg gctttctcat gacgtccgaa tgggccaaag
     2461 caaacgaggt tgcctgtgtg gcagtgactg tggcgatgca tttcctgttc ctggtggcgt
     2521 tcagttggat gcttgtggaa ggcctgcttt tgtggcggaa ggtagtcgcc gtgagcatgc
     2581 accccggacc tggaatgcgt ctgtaccacg ctaccggctg gggagtgcca gttggcattg
     2641 tggccgtgac gctggctatg ctgccccatg actacgtcgc tccgggccac tgctggctga
     2701 atgtgcatac caatgccatt tgggctttcg tcggacccgt gctgttcgtc ctgaccgcta
     2761 acacctgtat tctggcccgt gttgtcatga tcacggtgag ctcagcccgc cggcgcgccc
     2821 gcatgttgtc tccccagccg tgtctccagc aacagatctg gacccagatc tgggcgaccg
     2881 tcaagcccgt gctggtgctg ctccccgtgt tgggtctcac ctggctggca ggcatcctgg
     2941 tgcacctgtc ccccgcctgg gcgtacgcgg ctgttggcct gaactccatc caggggctgt
     3001 acatttttct ggtgtacgca gcttgcaatg aggaagtgcg ctccgctctg cagcgtatgg
     3061 cagaaaagaa agtcgcagaa gtgttgagag ccctgggcgt gtggggcggt gcggccaagg
     3121 agcacagcct gcccttcagt gttctgcctc tgttcctgcc tcccaagcct agcacccctc
     3181 gccatccgct caaggctccc gccggttctg actacaaaga cgatgacgac aagtgaagat
     3241 ctgacaaaac tcacacatgc ccaccgtgcc cagcacctga actcctgggg ggaccgtcag
     3301 tcttcctctt ccccccaaaa cccaaggaca ccctcatgat ctcccggacc cctgaggtca
     3361 catgcgtggt ggtggacgtg agccacgaag accctgaggt caagttcaac tggtacgtgg
     3421 acggcgtgga ggtgcataat gccaagacaa agccgcggga ggagcagtac aacagcacgt
     3481 accgtgtggt cagcgtcctc accgtcctgc accaggactg gctgaatggc aaggagtaca
     3541 agtgcaaggt ctccaacaaa gccctcccag cccccatcga gaaaaccatc tccaaagcca
     3601 aagggcagcc ccgagaacca caggtgtaca ccctgccccc atcccgggag gagatgacca
     3661 agaaccaggt cagcctgacc tgcctggtca aaggcttcta tcccagcgac atcgccgtgg
     3721 agtgggagag caatgggcag ccggagaaca actacaagac cacgcctccc gtgctggact
     3781 ccgacggctc cttcttcctc tacagcaagc tcaccgtgga caagagcagg tggcagcagg
     3841 ggaacgtctt ctcatgctcc gtgatgcacg aggctctgca caaccactac acgcagaaga
     3901 gcctctccct gtctccgggt aaatgagtgc tagctggcca gacatgataa gatacattga
     3961 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     4021 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     4081 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     4141 aaacctctac aaatgtggta tggaattaat tctaaaatac agcatagcaa aactttaacc
     4201 tccaaatcaa gcctctactt gaatcctttt ctgagggatg aataaggcat aggcatcagg
     4261 ggctgttgcc aatgtgcatt agctgtttgc agcctcacct tctttcatgg agtttaagat
     4321 atagtgtatt ttcccaaggt ttgaactagc tcttcatttc tttatgtttt aaatgcactg
     4381 acctcccaca ttcccttttt agtaaaatat tcagaaataa tttaaataca tcattgcaat
     4441 gaaaataaat gttttttatt aggcagaatc cagatgctca aggcccttca taatatcccc
     4501 cagtttagta gttggactta gggaacaaag gaacctttaa tagaaattgg acagcaagaa
     4561 agcgagcttc tagcttatcc tcagtcctgc tcctctgcca caaagtgcac gcagttgccg
     4621 gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc ggtcatggcc
     4681 ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc gtacagctcg
     4741 tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg gtcctggacc
     4801 gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag
     4861 tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg
     4921 gtgagcaccg gaacggcact ggtcaacttg gccatgatgg ctcctcctgt caggagagga
     4981 aagagaagaa ggttagtaca attgctatag tgagttgtat tatactatgc agatatacta
     5041 tgccaatgat taattgtcaa actagggctg cagggttcat agtgccactt ttcctgcact
     5101 gccccatctc ctgcccaccc tttcccaggc atagacagtc agtgacttac caaactcaca
     5161 ggagggagaa ggcagaagct tgagacagac ccgcgggacc gccgaactgc gaggggacgt
     5221 ggctagggcg gcttctttta tggtgcgccg gccctcggag gcagggcgct cggggaggcc
     5281 tagcggccaa tctgcggtgg caggaggcgg ggccgaaggc cgtgcctgac caatccggag
     5341 cacataggag tctcagcccc ccgccccaaa gcaaggggaa gtcacgcgcc tgtagcgcca
     5401 gcgtgttgtg aaatgggggc ttgggggggt tggggccctg actagtcaaa acaaactccc
     5461 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc
     5521 attgatgtac tgccaaaacc gcatcatcat ggtaatagcg atgactaata cgtagatgta
     5581 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt
     5641 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa
     5701 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt
     5761 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc
     5821 aggcgggcca tttaccgtaa gttatgtaac gcctgcaggt taattaagaa catgtgagca
     5881 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5941 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6001 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6061 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6121 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6181 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6241 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6301 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtgg
//
