LOCUS       sequence-430293-        7272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRL1 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..7272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        171..474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             1067..1441
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1455..1849
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     polyA_signal    1953..2074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1990..2009)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2044..2063
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(2789..2812)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5852..5878)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     sig_peptide     complement(5879..5938)
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     LTR             5988..6256
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(6269..6480)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    6517..6608
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(6549..6568)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    6619..6667
                     /note="synthetic polyadenylation signal"
     rep_origin      join(6775..7272,1..91)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     join(7264..7272,1..11)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
       61 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
      121 cctggccttt tgctggcctt ttgctcacat gttcttaatt aacctgcagg cgttacataa
      181 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      241 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      301 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      361 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      421 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catgatgatg
      481 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      541 ctccacccca ttgacgtcaa tgggagtttg ttttgactag tcagggcccc aaccccccca
      601 agcccccatt tcacaacacg ctggcgctac aggcgcgtga cttccccttg ctttggggcg
      661 gggggctgag actcctatgt gctccggatt ggtcaggcac ggccttcggc cccgcctcct
      721 gccaccgcag attggccgct aggcctcccc gagcgccctg cctccgaggg ccggcgcacc
      781 ataaaagaag ccgccctagc cacgtcccct cgcagttcgg cggtcccgcg ggtctgtctc
      841 aagcttctgc cttctccctc ctgtgagttt ggtaagtcac tgactgtcta tgcctgggaa
      901 agggtgggca ggagatgggg cagtgcagga aaagtggcac tatgaaccct gcagccctag
      961 tttgacaatt aatcattggc atagtatatc tgcatagtat aatacaactc actatagcaa
     1021 ttgtactaac cttcttctct ttcctctcct gacaggagga gccatcatgg ccaagttgac
     1081 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     1141 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     1201 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     1261 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     1321 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     1381 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttt gtggcagagg agcaggactg
     1441 aggataagct agaagctcgc tttcttgctg tccaatttct attaaaggtt cctttgttcc
     1501 ctaagtccaa ctactaaact gggggatatt atgaagggcc ttgagcatct ggattctgcc
     1561 taataaaaaa catttatttt cattgcaatg atgtatttaa attatttctg aatattttac
     1621 taaaaaggga atgtgggagg tcagtgcatt taaaacataa agaaatgaag agctagttca
     1681 aaccttggga aaatacacta tatcttaaac tccatgaaag aaggtgaggc tgcaaacagc
     1741 taatgcacat tggcaacagc ccctgatgcc tatgccttat tcatccctca gaaaaggatt
     1801 caagtagagg cttgatttgg aggttaaagt tttgctatgc tgtattttag aattaattcc
     1861 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     1921 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     1981 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2041 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggccagct agcactcatt
     2101 tacccggaga cagggagagg ctcttctgcg tgtagtggtt gtgcagagcc tcgtgcatca
     2161 cggagcatga gaagacgttc ccctgctgcc acctgctctt gtccacggtg agcttgctgt
     2221 agaggaagaa ggagccgtcg gagtccagca cgggaggcgt ggtcttgtag ttgttctccg
     2281 gctgcccatt gctctcccac tccacggcga tgtcgctggg atagaagcct ttgaccaggc
     2341 aggtcaggct gacctggttc ttggtcatct cctcccggga tgggggcagg gtgtacacct
     2401 gtggttctcg gggctgccct ttggctttgg agatggtttt ctcgatgggg gctgggaggg
     2461 ctttgttgga gaccttgcac ttgtactcct tgccattcag ccagtcctgg tgcaggacgg
     2521 tgaggacgct gaccacacgg tacgtgctgt tgtactgctc ctcccgcggc tttgtcttgg
     2581 cattatgcac ctccacgccg tccacgtacc agttgaactt gacctcaggg tcttcgtggc
     2641 tcacgtccac caccacgcat gtgacctcag gggtccggga gatcatgagg gtgtccttgg
     2701 gttttggggg gaagaggaag actgacggtc cccccaggag ttcaggtgct gggcacggtg
     2761 ggcatgtgtg agttttgtca gatcttcact tgtcgtcatc gtctttgtag tcagaaccca
     2821 gtgaggtgac caactgcatc tgtccatcac cgtcagggcc aggcccctcc aggccgggtg
     2881 cggcaagata gccctcatgg ctaggacggc gtacctggta ataaccttgc agtggattgc
     2941 gagcgaccag cgctggcgga cgactcgtat agtagatttc cggtgggcca ggtggagcag
     3001 gcggtggagg tgggagtgcc tcagatggtc cctcgggaga ggagtctgga tagctgggac
     3061 tatccctcag gtttgcgcca ctagcataca gggaatcgcg tccgggagga ctggacagag
     3121 gacgggaggt agctccatct tcagcggtgc aggattcact ctcatccagg tcggactgat
     3181 acagcacaga ctgcgcgcga ggcaggagca ggggctcttc aagggccttg tagagcagtt
     3241 cgatctcagc cctgtcggcc ccgcctggac cgccggcctc ttcctccccg cctccaccag
     3301 gcacaggtgg aacgggaggc tcaggcggag gcggtccttt cgcggcggaa gagctacctc
     3361 tcagattgtt gtgaaccaac tcgctaatga tcatcttttc gaacgcggca gcatcagcca
     3421 ggttacgacc ccttggaggt tctgggccac catcgccagg cggaaagtcg ccactccgca
     3481 gggaatagga gttattgaag ttaccgttaa gcggcagggt gtccatgccg caggcttctc
     3541 tgccacccag gggatgcttc ggctcccgat aagaaccggg gctattgaag acaggcggtg
     3601 agctcgggtt gaaccccaca gactccgcaa taagggtgtt gtagggactt gttccgccac
     3661 ggggctgcag tactgggttg gtaagcagat ggttgcccat ggtaccccgg ttgagggtcg
     3721 gggttgagtt gatgtcgcca gccataaaag aggactcggt ctgtttgcgc acggtgtcgt
     3781 tccacatacg ccgaatgcgg gactgggtac cggtatagta ccgggtgtta gaacgcatcg
     3841 cggatgtctt cagagatccg tgtgtgccac ctggcgggct ccgaatgcaa caatagctat
     3901 ggcgcaggca cttggaatat tccttgtgga cctttttctg cagcgcgcaa tggaaaacga
     3961 aaatgaacac gccctggaag gcgttgaagg tagtgaacag ataagccata acgacgcttt
     4021 ctttattgat gaacaggagg ccaaaggccc aggtcaaccc cagaagaaac aaaagcgcga
     4081 tggcgcccag cgcccaggac ttgatgttat ccagacggct ggaatcgggc ttcaacacgg
     4141 aactgctgcg gatcatcttg tgcagtgtca ccatcaggaa taccaagttc acgacgatga
     4201 caaaagaaac tggaccgatg aaggaccaaa tgaaatagtt atcgacgcgg agccaacaag
     4261 ccttctctgt cccgtaggat ctgtaatcga tggctgcggc gatgcccaca accagagctg
     4321 ggaaacagta gccacccagg taatagtact tggtgcggga atactcgctc tcaaagacct
     4381 ctaccaacag caagtagaga tggacaccct ccaggcacag ccaggaaaag gcagccagga
     4441 aaaaataatg caggagacca gcgaagatgg ggcaagcgat ttcgtactgg gtcttgtcga
     4501 tcccaaccag gaacaggagc tcagcgagga acaggttaat gcagaggttc ttgtgaatgg
     4561 tgttcctgtc ggtctgcagt cctctgagaa aacagaaagt ggagatacaa atggccaggc
     4621 acaccaggct gatcacaatg ccaacccatg tgatcacact aagcaggagc tcgttaatgc
     4681 ggccctggta gatttcgcgg tgggccatga gcacggcaaa gttggtttgg aagtaaaggt
     4741 tctcgttggt ctgcgcatct ttcagcgctt catccacggt ctggcggccg ctcgccgcgt
     4801 taatcaccgc ggtgcgcacc gcataccaaa acgcgctcat ctgcggaatg ttcggcataa
     4861 tttcgccttt ctgcgcgttt tccatggtcg ccgcaatgcg cggatctttc gccagttctt
     4921 cttcatagct tttcagcgcc accgcgccca gcggtttatc tttgttcacc gcttccaggc
     4981 cttcatcggt cagcagatag ttttccagaa attctttcgc cagttctttg ttcgggctcg
     5041 ccgcgttaat gcccgcgctc agcacgccca caaacggttt gctcggctgg cctttaaagg
     5101 tcggcagcac ggtcacgcca tagttcactt tgctggtatc aatgttgctc cacgcccacg
     5161 ggccgttaat ggtcatcgcg gtttcgcctt tgttaaacgc cgcttccgca atgctataat
     5221 cggtatccgc gttcatatgt ttgtttttaa tcagatccac cagaaaggtc aggcccgctt
     5281 tcgcgcccgc gttatccacg cccacatctt taatatcata tttgccgttt tcatatttaa
     5341 acgcatagcc gccatccgcc gcaatcagcg gccaggtaaa atacggttcc tgcaggttaa
     5401 acatcagcgc gcttttgcct ttcgctttca gttctttatc cagcgccgga atttcttccc
     5461 aggttttcgg cgggttcggc agcagatctt tgttataaat caggctcagc gcttccaccg
     5521 caatcggata cgcaatcagt ttgccgttat agcgcaccgc atcccaggta aacggataca
     5581 gtttatcctg aaacgcttta tccggggtaa tttccgccag caggccgctc tgcgcatagc
     5641 cgccaaagcg atcatgcgcc caaaaaataa tatccgggcc atcgccggtc gccgccacct
     5701 gcggaaattt ttcttccagt ttatccggat gttccacggt cactttaatg ccggtatctt
     5761 tttcaaattt tttgcccact tccgccaggc cgttatagcc tttatcgccg ttaatccaaa
     5821 tcaccagttt gccttcttca attttggatc cagcgtaatc tggaacatcg tatgggtacg
     5881 aattcgtgac aagtgcaaga cttagtgcaa tgcaagacag gagttgcatc ctgtacatgg
     5941 tggccctcct tcgccggtga tctcaggtag gcgccggtca cagcttggat ctgtaacggc
     6001 gcagaacaga aaacgaaaca aagacgtaga gttgagcaag cagggtcagg caaagcgtgg
     6061 agagccggct gagtctaggt aggctccaag ggagcgccgg acaaaggccc ggtctcgacc
     6121 tgagctttaa acttacctag acggcggacg cagttcagga ggcaccacag gcgggaggcg
     6181 gcagaacgcg actcaaccgg cgtggatggc ggcctcaggt agggcggcgg gcgcgtgaag
     6241 gagagatgcg agcccctcga agcttcagct gtgttctggc ggcaaacccg ttgcgaaaaa
     6301 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     6361 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcacccg
     6421 ttcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     6481 actgacgggc accggagcga tcgcagatcc ttcgatagag aaatgttctg gcacctgcac
     6541 ttgcactggg gacagcctat tttgctagtt tgttttgttt cgttttgttt tgatggagag
     6601 cgtatgttag ttacgattca cacaaaaaac caacacacag atgtaatgaa aataaagata
     6661 ttttattgcg gccgctgatt taaatgttaa ttaactagcc atgaccaaaa tcccttaacg
     6721 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     6781 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     6841 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     6901 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6961 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7021 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7081 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7141 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7201 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7261 agggggaaac gc
//
