LOCUS       sequence-430297-        7182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRL3 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..7182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(320..694)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        1287..1590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(1670..2258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1750..1769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    2366..2414
                     /note="synthetic polyadenylation signal"
     misc_feature    2425..2516
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2465..2484
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2553..2764
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2777..3045
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     3095..3154
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             3155..3181
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6131..6154
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    6869..6990
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6880..6899)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6934..6953
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    join(7094..7182,1..306)
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
ORIGIN
        1 gctgttgcca atgtgcatta gctgtttgca gcctcacctt ctttcatgga gtttaagata
       61 tagtgtattt tcccaaggtt tgaactagct cttcatttct ttatgtttta aatgcactga
      121 cctcccacat tcccttttta gtaaaatatt cagaaataat ttaaatacat cattgcaatg
      181 aaaataaatg ttttttatta ggcagaatcc agatgctcaa ggcccttcat aatatccccc
      241 agtttagtag ttggacttag ggaacaaagg aacctttaat agaaattgga cagcaagaaa
      301 gcgagcttct agcttatcct cagtcctgct cctctgccac aaagtgcacg cagttgccgg
      361 ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg
      421 gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt
      481 ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg
      541 cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt
      601 cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg
      661 tgagcaccgg aacggcactg gtcaacttgg ccatgatggc tcctcctgtc aggagaggaa
      721 agagaagaag gttagtacaa ttgctatagt gagttgtatt atactatgca gatatactat
      781 gccaatgatt aattgtcaaa ctagggctgc agggttcata gtgccacttt tcctgcactg
      841 ccccatctcc tgcccaccct ttcccaggca tagacagtca gtgacttacc aaactcacag
      901 gagggagaag gcagaagctt gagacagacc cgcgggaccg ccgaactgcg aggggacgtg
      961 gctagggcgg cttcttttat ggtgcgccgg ccctcggagg cagggcgctc ggggaggcct
     1021 agcggccaat ctgcggtggc aggaggcggg gccgaaggcc gtgcctgacc aatccggagc
     1081 acataggagt ctcagccccc cgccccaaag caaggggaag tcacgcgcct gtagcgccag
     1141 cgtgttgtga aatgggggct tgggggggtt ggggccctga ctagtcaaaa caaactccca
     1201 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     1261 ttgatgtact gccaaaaccg catcatcatg gtaatagcga tgactaatac gtagatgtac
     1321 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     1381 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     1441 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     1501 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     1561 ggcgggccat ttaccgtaag ttatgtaacg cctgcaggtt aattaagaac atgtgagcaa
     1621 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1681 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1741 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1801 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1861 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1921 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1981 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2041 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2101 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2161 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2221 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2281 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atggctagtt
     2341 aattaacatt taaatcagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     2401 tggttttttg tgtgaatcgt aactaacata cgctctccat caaaacaaaa cgaaacaaaa
     2461 caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg
     2521 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2581 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     2641 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2701 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2761 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     2821 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     2881 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     2941 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     3001 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     3061 gcctacctga gatcaccggc gaaggagggc caccatgtac aggatgcaac tcctgtcttg
     3121 cattgcacta agtcttgcac ttgtcacgaa ttcgtaccca tacgatgttc cagattacgc
     3181 tggatccaaa attgaagaag gcaaactggt gatttggatt aacggcgata aaggctataa
     3241 cggcctggcg gaagtgggca aaaaatttga aaaagatacc ggcattaaag tgaccgtgga
     3301 acatccggat aaactggaag aaaaatttcc gcaggtggcg gcgaccggcg atggcccgga
     3361 tattattttt tgggcgcatg atcgctttgg cggctatgcg cagagcggcc tgctggcgga
     3421 aattaccccg gataaagcgt ttcaggataa actgtatccg tttacctggg atgcggtgcg
     3481 ctataacggc aaactgattg cgtatccgat tgcggtggaa gcgctgagcc tgatttataa
     3541 caaagatctg ctgccgaacc cgccgaaaac ctgggaagaa attccggcgc tggataaaga
     3601 actgaaagcg aaaggcaaaa gcgcgctgat gtttaacctg caggaaccgt attttacctg
     3661 gccgctgatt gcggcggatg gcggctatgc gtttaaatat gaaaacggca aatatgatat
     3721 taaagatgtg ggcgtggata acgcgggcgc gaaagcgggc ctgacctttc tggtggatct
     3781 gattaaaaac aaacatatga acgcggatac cgattatagc attgcggaag cggcgtttaa
     3841 caaaggcgaa accgcgatga ccattaacgg cccgtgggcg tggagcaaca ttgataccag
     3901 caaagtgaac tatggcgtga ccgtgctgcc gacctttaaa ggccagccga gcaaaccgtt
     3961 tgtgggcgtg ctgagcgcgg gcattaacgc ggcgagcccg aacaaagaac tggcgaaaga
     4021 atttctggaa aactatctgc tgaccgatga aggcctggaa gcggtgaaca aagataaacc
     4081 gctgggcgcg gtggcgctga aaagctatga agaagaactg gcgaaagatc cgcgcattgc
     4141 ggcgaccatg gaaaacgcgc agaaaggcga aattatgccg aacattccgc agatgagcgc
     4201 gttttggtat gcggtgcgca ccgcggtgat taacgcggcg agcggccgcc agaccgtgga
     4261 tgaagcgctg aaagatgcgc agaccaacga gaacctttac ttccaaacca atttcgctgt
     4321 cttgatggcc catgtcgaag ttaaacactc cgatgcggtc catgacttgc tgttggatgt
     4381 catcacatgg gtgggtattc tgttgagcct ggtgtgtctg ctcatctgta tcttcacatt
     4441 ttgcttcttt aggggtctgc agagcgacag gaacacgatc cataaaaacc tgtgtatctc
     4501 actgttcgtg gcggaactcc tgttcctgat tggcattaac cgcactgatc agcctattgc
     4561 ctgcgcggtg ttcgctgccc tgcttcactt tttctttctg gcagccttca cttggatgtt
     4621 tctcgagggc gttcagctgt atatcatgct ggtcgaagtc ttcgaatctg agcattcacg
     4681 ccggaagtat ttctaccttg tgggctatgg aatgccagcc ctcatcgttg ccgtttccgc
     4741 cgctgtggac taccgtagct acgggacaga taaggtgtgc tggctgaggc tggacacata
     4801 cttcatctgg agtttcatcg gccccgctac cctgattatc atgctgaacg tcatcttcct
     4861 gggcatcgcc ctgtacaaaa tgtttcacca tacagctatc ctcaagccgg aatctggctg
     4921 tctcgacaac attaagtcct gggttatcgg tgctatcgct ttgctgtgcc ttttgggtct
     4981 gacttgggca ttcggcctga tgtacattaa cgaatccaca gtcatcatgg cctacctgtt
     5041 taccatcttc aacagcctgc agggcatgtt catctttatt ttccactgtg tgctccagaa
     5101 aaaggtgcgg aaagaatatg ggaaatgtct gcgtactcac tgctgtagcg gcaagtccac
     5161 ggagtcttcc attggatccg gcaagaccag tggtagccgc acacccggaa gatactccac
     5221 tggttcccag tctagaatcc gtcgcatgtg gaatgacacc gtgagaaaac agagcgagag
     5281 tagcttcatc acgggcgaca tcaactccag tgccagtctg aacagggagg gactcctgaa
     5341 taacgctcgc gacacctccg tcatggacac cttgcccctg aacggcaacc acggcaactc
     5401 atacagcatc gcttccggcg agtatctctc caactgcgtt cagatcattg atcgcggcta
     5461 caaccataac gagactgctc tcgagaaaaa gatcctgaag gaactgacca gcaattacat
     5521 tcccagctat ctgaataacc acgaacgtag ttccgagcaa aacagaaatc ttatgaacaa
     5581 actcgtgaat aacctggggt ccgggagaga agatgacgct atcgtgcttg acgatgctac
     5641 ctcctttaac cacgaagagt ccttgggcct cgagctgatc cacgaagaga gcgatgcccc
     5701 attgctgcca cctcgggtct acagcacaga gaaccaccag ccgcaccatt acacccgtag
     5761 gagaatccca caggatcact ccgagtcctt tttccctctg ctcactaacg aacacaccga
     5821 ggatctgcag agcccccatc gggacagttt gtatactagc atgcccacac tcgctggcgt
     5881 ggcggcaaca gaatccgtaa ccactagcac ccagacggaa cctccgcccg cgaagtgcgg
     5941 cgatgccgag gatgtgtatt acaagagcat gccaaacttg ggttcccgca accacgtgca
     6001 ccagctgcat acctattacc agctgggtag gggtagtagc gacggtttta tcgtcccgcc
     6061 caacaaggat ggcacgcccc ctgaaggctc cagcaagggc cctgctcatc tggttacctc
     6121 cctgggttct gactacaaag acgatgacga caagtgaaga tctgacaaaa ctcacacatg
     6181 cccaccgtgc ccagcacctg aactcctggg gggaccgtca gtcttcctct tccccccaaa
     6241 acccaaggac accctcatga tctcccggac ccctgaggtc acatgcgtgg tggtggacgt
     6301 gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa
     6361 tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct
     6421 caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa
     6481 agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc
     6541 acaggtgtac accctgcccc catcccggga ggagatgacc aagaaccagg tcagcctgac
     6601 ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca
     6661 gccggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct
     6721 ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc
     6781 cgtgatgcac gaggctctgc acaaccacta cacgcagaag agcctctccc tgtctccggg
     6841 taaatgagtg ctagctggcc agacatgata agatacattg atgagtttgg acaaaccaca
     6901 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     6961 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     7021 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     7081 atggaattaa ttctaaaata cagcatagca aaactttaac ctccaaatca agcctctact
     7141 tgaatccttt tctgagggat gaataaggca taggcatcag gg
//
