LOCUS       sequence-430303-        7161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRL3 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..7161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    328..449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(339..358)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     393..412
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    553..947
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     CDS             complement(961..1335)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     enhancer        1928..2231
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2311..2899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2391..2410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3007..3055
                     /note="synthetic polyadenylation signal"
     misc_feature    3066..3157
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3106..3125
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3194..3405
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3418..3686
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     sig_peptide     3736..3795
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             3796..3822
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4930..4950
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQA"
     CDS             6751..6774
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 caggtgtaca ccctgccccc atcccgggag gagatgacca agaaccaggt cagcctgacc
       61 tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag caatgggcag
      121 ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc cttcttcctc
      181 tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt ctcatgctcc
      241 gtgatgcacg aggctctgca caaccactac acgcagaaga gcctctccct gtctccgggt
      301 aaatgagtgc tagctggcca gacatgataa gatacattga tgagtttgga caaaccacaa
      361 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      421 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      481 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
      541 tggaattaat tctaaaatac agcatagcaa aactttaacc tccaaatcaa gcctctactt
      601 gaatcctttt ctgagggatg aataaggcat aggcatcagg ggctgttgcc aatgtgcatt
      661 agctgtttgc agcctcacct tctttcatgg agtttaagat atagtgtatt ttcccaaggt
      721 ttgaactagc tcttcatttc tttatgtttt aaatgcactg acctcccaca ttcccttttt
      781 agtaaaatat tcagaaataa tttaaataca tcattgcaat gaaaataaat gttttttatt
      841 aggcagaatc cagatgctca aggcccttca taatatcccc cagtttagta gttggactta
      901 gggaacaaag gaacctttaa tagaaattgg acagcaagaa agcgagcttc tagcttatcc
      961 tcagtcctgc tcctctgcca caaagtgcac gcagttgccg gccgggtcgc gcagggcgaa
     1021 ctcccgcccc cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa
     1081 gttcgtggac acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac
     1141 ccaggccagg gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac
     1201 gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg
     1261 gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact
     1321 ggtcaacttg gccatgatgg ctcctcctgt caggagagga aagagaagaa ggttagtaca
     1381 attgctatag tgagttgtat tatactatgc agatatacta tgccaatgat taattgtcaa
     1441 actagggctg cagggttcat agtgccactt ttcctgcact gccccatctc ctgcccaccc
     1501 tttcccaggc atagacagtc agtgacttac caaactcaca ggagggagaa ggcagaagct
     1561 tgagacagac ccgcgggacc gccgaactgc gaggggacgt ggctagggcg gcttctttta
     1621 tggtgcgccg gccctcggag gcagggcgct cggggaggcc tagcggccaa tctgcggtgg
     1681 caggaggcgg ggccgaaggc cgtgcctgac caatccggag cacataggag tctcagcccc
     1741 ccgccccaaa gcaaggggaa gtcacgcgcc tgtagcgcca gcgtgttgtg aaatgggggc
     1801 ttgggggggt tggggccctg actagtcaaa acaaactccc attgacgtca atggggtgga
     1861 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     1921 gcatcatcat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     1981 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     2041 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     2101 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     2161 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     2221 gttatgtaac gcctgcaggt taattaagaa catgtgagca aaaggccagc aaaaggccag
     2281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2821 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2881 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2941 gaacgaaaac tcacgttaag ggattttggt catggctagt taattaacat ttaaatcagc
     3001 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     3061 taactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
     3121 gtccccagtg caagtgcagg tgccagaaca tttctctatc gaaggatctg cgatcgctcc
     3181 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     3241 gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     3301 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     3361 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg
     3421 gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg
     3481 agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta
     3541 agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga
     3601 ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg
     3661 ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacctg agatcaccgg
     3721 cgaaggaggg ccaccatgta caggatgcaa ctcctgtctt gcattgcact aagtcttgca
     3781 cttgtcacga attcgtaccc atacgatgtt ccagattacg ctggatccaa aattgaagaa
     3841 ggcaaactgg tgatttggat taacggcgat aaaggctata acggcctggc ggaagtgggc
     3901 aaaaaatttg aaaaagatac cggcattaaa gtgaccgtgg aacatccgga taaactggaa
     3961 gaaaaatttc cgcaggtggc ggcgaccggc gatggcccgg atattatttt ttgggcgcat
     4021 gatcgctttg gcggctatgc gcagagcggc ctgctggcgg aaattacccc ggataaagcg
     4081 tttcaggata aactgtatcc gtttacctgg gatgcggtgc gctataacgg caaactgatt
     4141 gcgtatccga ttgcggtgga agcgctgagc ctgatttata acaaagatct gctgccgaac
     4201 ccgccgaaaa cctgggaaga aattccggcg ctggataaag aactgaaagc gaaaggcaaa
     4261 agcgcgctga tgtttaacct gcaggaaccg tattttacct ggccgctgat tgcggcggat
     4321 ggcggctatg cgtttaaata tgaaaacggc aaatatgata ttaaagatgt gggcgtggat
     4381 aacgcgggcg cgaaagcggg cctgaccttt ctggtggatc tgattaaaaa caaacatatg
     4441 aacgcggata ccgattatag cattgcggaa gcggcgttta acaaaggcga aaccgcgatg
     4501 accattaacg gcccgtgggc gtggagcaac attgatacca gcaaagtgaa ctatggcgtg
     4561 accgtgctgc cgacctttaa aggccagccg agcaaaccgt ttgtgggcgt gctgagcgcg
     4621 ggcattaacg cggcgagccc gaacaaagaa ctggcgaaag aatttctgga aaactatctg
     4681 ctgaccgatg aaggcctgga agcggtgaac aaagataaac cgctgggcgc ggtggcgctg
     4741 aaaagctatg aagaagaact ggcgaaagat ccgcgcattg cggcgaccat ggaaaacgcg
     4801 cagaaaggcg aaattatgcc gaacattccg cagatgagcg cgttttggta tgcggtgcgc
     4861 accgcggtga ttaacgcggc gagcggccgc cagaccgtgg atgaagcgct gaaagatgcg
     4921 cagaccaacg agaaccttta cttccaagcc catgtcgaag ttaaacactc cgatgcggtc
     4981 catgacttgc tgttggatgt catcacatgg gtgggtattc tgttgagcct ggtgtgtctg
     5041 ctcatctgta tcttcacatt ttgcttcttt aggggtctgc agagcgacag gaacacgatc
     5101 cataaaaacc tgtgtatctc actgttcgtg gcggaactcc tgttcctgat tggcattaac
     5161 cgcactgatc agcctattgc ctgcgcggtg ttcgctgccc tgcttcactt tttctttctg
     5221 gcagccttca cttggatgtt tctcgagggc gttcagctgt atatcatgct ggtcgaagtc
     5281 ttcgaatctg agcattcacg ccggaagtat ttctaccttg tgggctatgg aatgccagcc
     5341 ctcatcgttg ccgtttccgc cgctgtggac taccgtagct acgggacaga taaggtgtgc
     5401 tggctgaggc tggacacata cttcatctgg agtttcatcg gccccgctac cctgattatc
     5461 atgctgaacg tcatcttcct gggcatcgcc ctgtacaaaa tgtttcacca tacagctatc
     5521 ctcaagccgg aatctggctg tctcgacaac attaagtcct gggttatcgg tgctatcgct
     5581 ttgctgtgcc ttttgggtct gacttgggca ttcggcctga tgtacattaa cgaatccaca
     5641 gtcatcatgg cctacctgtt taccatcttc aacagcctgc agggcatgtt catctttatt
     5701 ttccactgtg tgctccagaa aaaggtgcgg aaagaatatg ggaaatgtct gcgtactcac
     5761 tgctgtagcg gcaagtccac ggagtcttcc attggatccg gcaagaccag tggtagccgc
     5821 acacccggaa gatactccac tggttcccag tctagaatcc gtcgcatgtg gaatgacacc
     5881 gtgagaaaac agagcgagag tagcttcatc acgggcgaca tcaactccag tgccagtctg
     5941 aacagggagg gactcctgaa taacgctcgc gacacctccg tcatggacac cttgcccctg
     6001 aacggcaacc acggcaactc atacagcatc gcttccggcg agtatctctc caactgcgtt
     6061 cagatcattg atcgcggcta caaccataac gagactgctc tcgagaaaaa gatcctgaag
     6121 gaactgacca gcaattacat tcccagctat ctgaataacc acgaacgtag ttccgagcaa
     6181 aacagaaatc ttatgaacaa actcgtgaat aacctggggt ccgggagaga agatgacgct
     6241 atcgtgcttg acgatgctac ctcctttaac cacgaagagt ccttgggcct cgagctgatc
     6301 cacgaagaga gcgatgcccc attgctgcca cctcgggtct acagcacaga gaaccaccag
     6361 ccgcaccatt acacccgtag gagaatccca caggatcact ccgagtcctt tttccctctg
     6421 ctcactaacg aacacaccga ggatctgcag agcccccatc gggacagttt gtatactagc
     6481 atgcccacac tcgctggcgt ggcggcaaca gaatccgtaa ccactagcac ccagacggaa
     6541 cctccgcccg cgaagtgcgg cgatgccgag gatgtgtatt acaagagcat gccaaacttg
     6601 ggttcccgca accacgtgca ccagctgcat acctattacc agctgggtag gggtagtagc
     6661 gacggtttta tcgtcccgcc caacaaggat ggcacgcccc ctgaaggctc cagcaagggc
     6721 cctgctcatc tggttacctc cctgggttct gactacaaag acgatgacga caagtgaaga
     6781 tctgacaaaa ctcacacatg cccaccgtgc ccagcacctg aactcctggg gggaccgtca
     6841 gtcttcctct tccccccaaa acccaaggac accctcatga tctcccggac ccctgaggtc
     6901 acatgcgtgg tggtggacgt gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg
     6961 gacggcgtgg aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg
     7021 taccgtgtgg tcagcgtcct caccgtcctg caccaggact ggctgaatgg caaggagtac
     7081 aagtgcaagg tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaagcc
     7141 aaagggcagc cccgagaacc a
//
