LOCUS       sequence-430332-        9577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  shRNAmir backbone for RNA interference, with mTagBFP2 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Wang D, Xiu J, Zhao J, Luo J
  TITLE     miR-AB, a miRNA-based shRNA viral toolkit for multicolor-barcoded 
            multiplex RNAi at a single-cell level.
  JOURNAL   EMBO Rep. 2022 Apr 5;23(4):e53691. doi: 10.15252/embr.202153691. 
            Epub 2022 Feb 24.
  PUBMED    35201651
REFERENCE   2  (bases 1 to 9577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2022-04-5"; volume: "23(4):e53691. doi"; pages: " 
            10.15252/embr.202153691. Epub 2022 Feb 24"
COMMENT     Sequence Label: LEAPTW
FEATURES             Location/Qualifiers
     source          1..9577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2185..2396
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2397..3339
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3296..3316
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4430..4929
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4465..4488)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4862..4881
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4947..5657
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(5721..5741)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6139..6150)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6340..6973
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7102..7118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7102..7118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7115..7137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7126..7142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7150..7156,7157..7174,7175..7180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7195..7216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7333..7350)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7504..8092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7584..7603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8263..9054,9055..9123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8886..8905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9124..9228)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9276..9410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9313..9332)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9367..9386
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgggcag agcgcacatc gcccacagtc cccgagaagt
     2221 tggggggagg ggtcggcaat tgaaccggag cctagagaag gtggcgcggg gtaaactggg
     2281 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     2341 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     2401 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     2461 gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     2521 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     2581 gaggcctggc ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     2641 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     2701 tttttctggc aagatagtct tgtaaatgcg ggccaagatc ggcacactgg tatttcggtt
     2761 tttggggccg cgggcggcga cggggccggt gcgtcccagc gcacatgttc ggcgaggcgg
     2821 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     2881 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     2941 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     3001 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     3061 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     3121 cacctcgatt agttctcgcg cttttggagt acgtcgtctt taggttgggg ggaggggttt
     3181 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     3241 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     3301 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cgcttcgact
     3361 agggataaca gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca
     3421 catcttggaa acacttgctg ggattacttc gacttcttaa cccaacagaa ggctcgagaa
     3481 ggtatattgc tgttgacagg atccacatta acaactttaa gtccagcaat ttgagttaag
     3541 ggtgttgctc tcaatgattt cactaatggt tcaattttta atttcttttc ttctggttca
     3601 aaattcaagt ttcaagtgaa agtgtaatat gcacccattt ctttcaataa atcttctaaa
     3661 tagtccacta atgttttatt ttgtttttta caaaatcaag cagcctctgc tatcaatata
     3721 gaaccttgta ttccatcttt atctctagct gagtcatcaa ttacatatcc ataactttct
     3781 tcataagcaa aaacaaaatt taatccgtta tcttcttctt tagcaatttc tctacccatt
     3841 catttcaatc cagttaaagt ttttacaatc ttaactccat atttttcctg agcgattcta
     3901 tcacccaaat cacttgttac aaaacttgaa tatagagccg gattttttgg aatgctattt
     3961 aagcgtttta gatttgataa ttttcaatca attaaaattg gtcctgtttg atttccatct
     4021 aatcttacaa aatgaccatc atgttttatt gccattccaa atctgtcagc atctgggtca
     4081 ttcataataa taatatctgc atcatgttta ataccatatt caagcggtat ttttcatgca
     4141 ggatcaaatt ctggatttgg atttacaaca tttttcaatg tttcatcttc aaatgcacga
     4201 tcttcaccct caataacgtt atatcctgat tcacgtaatc tttttggggt aaatttagtt
     4261 cctgttccat taactgcgct aaaaataggg ccctgcctcg gacttcaagg ggctagaatt
     4321 cgagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac
     4381 aaagctgaat taaaatggta taaattaaat cacttttttc aattctaccg ggtaggggag
     4441 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     4501 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     4561 ccgttctttg gtggcccctt cgcgccacct tctacccccc ccctagtcag gaagtccccc
     4621 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     4681 ctcgtgcaga tggacagcac cgctgagcaa tggaggcggg tgggcctttg gggcagcggc
     4741 caatagcagc tttgctcctt cgctttcggg gctcagaggc ggggaggggg tgggtccggg
     4801 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     4861 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     4921 ggcctttcga cctttaatta accaccatgg tgtctaaggg cgaagagctg attaaggaga
     4981 acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat
     5041 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     5101 gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga
     5161 ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct
     5221 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     5281 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat
     5341 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggcgttcacc gagacgctgt
     5401 accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga
     5461 gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca
     5521 agatgcctgg cgtgtactat gtggactaca gactggaaag aatcaaggag gccaacaacg
     5581 agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac
     5641 tggggcacaa gctaaattag gtttaaacat caacctctgg attacaaaat ttgtgaaaga
     5701 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5761 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5821 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5881 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5941 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6001 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6061 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6121 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6181 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6241 tgggccgcct ccccgcctgg cctgcagggg tacctttaag accaatgact tacaaggcag
     6301 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     6361 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     6421 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     6481 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     6541 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     6601 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     6661 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     6721 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     6781 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6841 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6901 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6961 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7021 aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag
     7081 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7261 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7321 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7381 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7441 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7501 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7561 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7621 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7681 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7801 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7861 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7921 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7981 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8041 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8101 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8161 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8221 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8281 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8341 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8401 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8461 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8521 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8581 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8641 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8701 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8761 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8821 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8881 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8941 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9001 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9061 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9241 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg
     9301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     9421 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     9481 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     9541 attgtatttg ttaaatatgt actacaaact tagtagt
//
