LOCUS       sequence-430340-       20582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FrCas9 cytosine base editing plasmid V2.1 in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20582)
  AUTHORS   He Y, Han Y, Ma Y, Liu S, Fan T, Liang Y, Tang X, Zheng X, Wu Y, 
            Zhang T, Qi Y, Zhang Y
  TITLE     Expanding plant genome editing scope and profiles with CRISPR-FrCas9
            systems targeting palindromic TA sites.
  JOURNAL   Plant Biotechnol J. 2024 Sep;22(9):2488-2503. doi: 
            10.1111/pbi.14363. Epub 2024 May 7.
  PUBMED    38713743
REFERENCE   2  (bases 1 to 20582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14363";
            journalName: "Plant Biotechnol J"; date: "2024-09"; volume: "22"; 
            issue: "9"; pages: "2488-2503"
COMMENT     Sequence Label: pGEL848
FEATURES             Location/Qualifiers
     source          1..20582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(33..38,39..56,57..63)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    71..87
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     95..111
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        158..2155
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             2816..2881
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2888..2908
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7046..7093
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7115..7363
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7376..7396
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      7412..7660
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(9310..9717)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     9582..9598
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9608..9627
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9608..9626
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9674..9694)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9674..9692)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9713..9729)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9737..9753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9761..9767,9768..9785,9786..9791))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9806..9827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10398..10414)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10617..10641
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11942..12571
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13000..14073
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14139..14333
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14569..14591)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14677..14817
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14732..14751
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14832..14849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15003..15591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15083..15102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17016..17810)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    18235..18259
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    18337..18511
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(18552..19577)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             complement(18552..19577)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(19644..20320)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(19687..19707)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    20511..20532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 wrwngtkagc ksaywmatta kkcaccccag gctttacact ttatgcttcc ggctcgtatg
       61 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
      121 aattcgagct cggtacccgg ggatcggcgc gccggagctg cagtgcagcg tgacccggtc
      181 gtgcccctct cttgagataa ggtgagcatt gcatgtctaa gttataaaaa attaccacat
      241 attttttttg tcacacttgt ttgaagtgca gtttatctat ctttatacat atatttaaac
      301 tttactctac gaataatata atctatagta ctacaataat atcagtgttt tagagaatca
      361 tataaatgaa cagttagaca tggtctaaag gacaattgag tattttgaca acaggactct
      421 acagttttat ctttttagtg tgcatgtgtt ctcctttttt tttgcaaata gcttcaccta
      481 tataatactt catccatttt attagtacat ccatttaggg tttagggtta atggttttta
      541 tagactaatt tttttagtac atctatttta ttctatttta gcctctaaat taagaaaact
      601 aaaactctat tttagttttt ttatttaata atttagatat aaaatagaat aaaataaagt
      661 gactaaaaat taaacaaata ccctttaaga aattaaaaaa actaaggaaa catttttctt
      721 gtttcgagta gataatgcca gcctgttaaa cgccgtcgac gagtctaacg gacaccaacc
      781 agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac ggcacggcat ctctgtcgct
      841 gcctctggac ccctctcgag agttccgctc caccgttgga cttgctccgc tgtcggcatc
      901 cagaaattgc gtggcggagc ggcagacgtg agccggcacg gcaggcggcc tcctcctcct
      961 ctcacggcac cggcagctac gggggattcc tttcccaccg ctccttcgct ttcccttcct
     1021 cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct cgtgttgttc
     1081 ggagcgcaca cacacacaac cagaactccc ccaaatccac ccgtcggcac ctccgcttca
     1141 aggtacgccg ctcgtcctcc cccccccccc tctctacctt ctcaagatcg gcgttccggt
     1201 ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg
     1261 ttagatccgt gctactagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga
     1321 ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag
     1381 acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc
     1441 tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt
     1501 cttggttgtg atgatgtggt ctggttgggc ggtcgttcaa gatcggagta gaattaattc
     1561 tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat
     1621 tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt
     1681 gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg
     1741 tggtgtggtt gggcggtcgt tcattcgttc aagatcggag tagaatactg tttcaaacta
     1801 cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga
     1861 gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact
     1921 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta
     1981 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg
     2041 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc
     2101 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcctgc
     2161 aggaatggag gcctctccgg cttcaggtcc tcgccatctc atggaccccc atatctttac
     2221 ctccaatttc aataacggca ttggcaggca caagacatac ctctgttatg aggtcgagcg
     2281 gctcgataac ggaacctcag tgaagatgga ccaacacagg ggtttccttc ataaccaagc
     2341 gaaaaatctc ctttgcggtt tctatgggcg gcacgcggag ctgagattct tggatttggt
     2401 cccgagcctc cagctggatc ccgcgcaaat ataccgcgtc acttggttca tatcatggag
     2461 cccgtgcttt agctgggggt gtgccggtga ggttcgcgcg ttcctccagg aaaacaccca
     2521 tgttcgcctc cggattttcg cagcgaggat tttcgactat gatccacttt acaaagaggc
     2581 cctccaaatg cttagagatg ctggggctca agtttcaatt atgacatacg acgagtttaa
     2641 gcattgttgg gacacttttg tcgatcatca gggttgtccc ttccagccgt gggatggtct
     2701 tgatgagcat tcgcaagctt tgagtggcag gcttcgcgcg atacttcaga accagggtaa
     2761 ctcaggttct gaaacccctg gcacaagtga gtcagcaaca cccgagtcca ccatggatta
     2821 caaggaccac gacggggatt acaaggacca cgacattgat tacaaggatg atgatgacaa
     2881 gatggctccg aagaagaaga ggaaggttgg catccacggg gtgccagctg cttgcaccaa
     2941 ggagtcggaa aagctcaata agaacgcgga ttactacatt ggtctggaca tgggcacatc
     3001 ctcagctggc tgggcggtga gtgactccga gtacaatcta atccgcagaa aaggcaagga
     3061 tttgtggggc gttcgccagt tcgaagaagc gaaaacggcg gcggaacgcc gggggttccg
     3121 ggtcgctcgt cgaaggaaac agaggcagca ggtcaggaat cgactattga gtgaggaatt
     3181 tcagaatgaa atcacaaaga tcgattctgg gtttttaaaa agaatggagg attctcggtt
     3241 tgttattagc gacaagaggg tgccagagaa gtacaccctg ttcaatgatt ccgggtacac
     3301 agatgtagaa tactacaacc agtacccgac catctaccat ctgagaaaag ccttaatcga
     3361 atccaatgag aggtttgata ttcgccttgt attcctggga atccactcgt tgttccagca
     3421 tccaggccat ttcctcgaca agggggacgt ggatacggac aatacaggtc ccgaagaact
     3481 gatacagttt ctcgaagact gcatgaacga gatacaaatc agcatcccgc tcgtctcaaa
     3541 ccaaaaagtc ttaactgata tcttgacgga tagccgaata acgaggcgtg acaaagaaca
     3601 gcaaattctt gagatattgc aacccaacaa agagtccaaa aaggccgttt cccagttcgt
     3661 gaaggttctc acaggtcaaa aagccaagtt aggcgatctc attatgatgg aagataaaga
     3721 tacggaggag tacaagtatt cattttcttt ccgcgaaaag accctggagg agattcttcc
     3781 cgatatagag ggcgtcatcg acggtcttgc gcttgagtat atcgaatcta tctactcgct
     3841 atattcatgg agcctcctca acagctacat gaaagataca ttgaccggtc attattactc
     3901 gtatctagct gaggcccgcg tggcggcgta cgataagcat catagtgatc ttgttaagct
     3961 gaaaacattg ttccgcgagt atatcccgga agaatacgac aacttcttcc gcaagatgga
     4021 gaaggccaac tattcgcatt atattggcag caccgagtat gatggtgaga agcgttgtag
     4081 aacagccaaa gccaagcaag aggacttcta caagagtatc aacaaaatgc tcgaaaagat
     4141 ccctgagtgc agtgaaaaaa cggagatcca gaaggagata atagagggca ctttcctcct
     4201 taagcaaact gggcctcaga acggctttgt cccgaatcag ttacagctaa aagaactacg
     4261 aaagatcctg caaaatgcgt ccaagcacta cccctttcta acagagaagg atgaaagaga
     4321 tatgactgcc attgacagaa tcgaggcgct gttcagcttt agaatcccgt actacatcgg
     4381 cccactcaag aacacggaca atcaagggca cggttgggct gttaggaggg acgggcacga
     4441 gcaaatcccg gtacggccgt ggaacttcga agaaatcatt gatgagtccg ctagcgccga
     4501 tctgttcatc aagaatttgg tgaacagttg tacttacctc aggaccgaga aggtactccc
     4561 aaaatctagc ttgctgtatc aagaattcga agtcctgaat gagctcaaca acctcaggat
     4621 aaatgggatg tacccagatg aaattcagcc aggcctaaag cgtatgatct tcgagcagtg
     4681 cttctattct ggcaaaaaag tcactggaaa aaaactgcag ttattcctca ggagtgtgct
     4741 caccaattct tcaacggagg agtttgttct aacaggcatt gacaaggact ttaaatcgtc
     4801 cctgtcgagt tacaaaaaat tctgcgagct ttttggcgtt aagacactga acgatacaca
     4861 gaaggtgatg gctgaacaga ttattgaatg gtccactgtc tatggcgatt cgcgcaagtt
     4921 tcttaagaga aagctcgagg ataactatcc ggaacttaca gatcaacaaa ttcgacggat
     4981 tgctggcttc aagttctccg agtgggggaa cctgagcagg gcattcctcg agatggaggg
     5041 gtacaaggac gaagcaggaa atcctgttac cataattaga gcgcttcgtg atactcaaaa
     5101 aaacttgatg cagctcctct ccaacgactc agcattcgct aagaagctgc aggagttaaa
     5161 tgactacgtg acgcgcgaca tctggtcaat agagccggac gacctcgatg gaatgtatct
     5221 gtctgctcct gttcgccgga tgatatggca gacatttttg atcctcagag aagtggtcga
     5281 taccattggt tattcaccta agaagatttt catggcgatg gcgcgggggg agcaggagaa
     5341 gaagaggact gcgtctagga agaaacagtt gatagatctc tacaaggagg caggcatgaa
     5401 gaacgatgaa ttgtttggag atctcgagag cctggaagag gcccaattga ggtccaagaa
     5461 gctctatctt tatttccggc agatggggag ggatatttat agtggaaagc taattgattt
     5521 catggatgta ctccacggca atcggtacga catagaccac atccacccgc aaagcaagaa
     5581 gaaagatgac tccttagaaa acaatctggt actcacaagc aaggacttca ataatcatat
     5641 taaacaagac gtctacccca ttcctgagca aattcaatcg agacaaaaag gattttgggc
     5701 catgctcctg aagcaaggtt tcatgtctca ggagaaatac aaccgactca tgcggacgac
     5761 tccgttcacc gatgaggagc tggcggagtt cgtcaacaga caactggtgg agactagaca
     5821 gggaacaaaa gcaattatat cactcatcaa ccagtgtttc cctgactcag aagttgtgta
     5881 cgtgaaggcc ggaaacacct cggacttccg gcagcggttc gacattccaa agtcgcggga
     5941 cttgaacaat taccaccacg ccgtagacgc ctatcttaac atcgttgtcg gcaacgttta
     6001 tgacaccaaa tttaccaaaa atccaatcaa ttttattaag aagatgagga aatctggtaa
     6061 tttgcactcc tatagtttaa gaaggatgta tgatttcaat gtccagcgcg gagatcagac
     6121 cgcgtgggtt gccgaaaatg acacgacgtt aaaaacagtg aagaagacag cttttaagac
     6181 ctcgcccatg gtgacaaaac ggacgtacga gaggaaaggt gggttggcag attccgtcct
     6241 gatagccgcc aagaaggcaa agcctggggt ccatcttcca gtgaaaactt cagactcacg
     6301 ctttgcaaac caggtgtcca cctacggcgg ctacgacaac gtgaagggaa gccacttctt
     6361 tcttgttgag catcagcaga agaaaaaaac aataaggagc atagagaatg tcccgatcca
     6421 tcttaaagag aagttaaaga ccaaggagga actcgaacac tactgtgcac aagtgctcgg
     6481 catggtgcag cctgatgtcc gtctgactcg catcccaatg tactctcttc tattgattga
     6541 tggctattac tattacctga ccggacgtac gggtggcaat ctgtctctct ctaatgcggt
     6601 tgagctctgc ttgccagcca aggagcaggc acatatcagg atgatttcaa aaatagccgg
     6661 ggggcgctca acggacgctt tgtctgcgga ggccaaggac gattttcgca agaaaaatct
     6721 tcggctctat gacgaactcg ctgaaaagca ccggtcaact attttttcga aacgcaagaa
     6781 ccccatcgga cccaagctgc tgaagtacag agaggcattt gtgaaacaaa ctatcgagaa
     6841 tcaatgcaag gttattttac agatattgaa gttgaccagc acaaactgca aaacttctgc
     6901 tgaccttaag cttatcggag gttcagggca agaaggcgtc atgagtattt ccaagctcct
     6961 acgagctgaa aagtatgcag aattttacct aatttgtcag tcccctagcg gaatttatga
     7021 gactcgaaag aacctgctga ctataaagcg gccagcggcg acgaagaagg cggggcaggc
     7081 gaagaagaag aagggagacg gatcaggcgg gtcaacaaac ctcagtgaca tcatagagaa
     7141 ggaaactggt aagcaactgg ttattcaaga gagcattctg atgctccctg aggaagtcga
     7201 ggaagttata ggaaacaagc ctgagagcga tatactcgtg catacggcgt atgacgagag
     7261 cacggatgaa aatgtgatgc tcttgaccag cgatgcgcca gaatataaac cctgggcact
     7321 ggttattcag gactctaacg gggaaaataa aatcaagatg ctctcaggtg gctccccaaa
     7381 gaagaaacgc aaggtttagg cttctcgagt atatgaagat gaagatgaaa tatttggtgt
     7441 gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
     7501 atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat
     7561 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta
     7621 tgtgctatgg tatggactat ggaatatgat taaagataag cgctgagctc ggacggcgcg
     7681 ccggagcaca tcagtctctg cacaaagtgc atcctgggct gcttcaatta taaagcccca
     7741 ttcaccacat ttgctagata gtcgaaaagc accatcaata ttgagcttca ggtatttttg
     7801 gttgtgttgt ggttggattg attctaatat ataccaaatc aatataattc actaccaaaa
     7861 tataccatag ccatcacaac tttattaatt ttggtagctt aagatggtat atataataac
     7921 caattaacaa ctgattctaa ttttactacg gcccagtatc taccaataca aaacaacgag
     7981 tatgttttct tccgtcgtaa tcgtacacag tacaaaaaaa cctggccagc ctttcttggg
     8041 ctggggctct ctttcgaaag gtcacaaaac gtacacggca gtaacgccgc ttcgctgcgt
     8101 gttaacggcc accaaccccg ccgtgagcaa acggcatcag ctttccacct cctcgatatc
     8161 tccgcggcgc cgtctggacc cgcccccttt ccgttccttt ctttccttct cgcgtttgcg
     8221 tggtggggac ggactcccca aaccgcctct ccctctcttt atttgtctat attctcactg
     8281 ggccccaccc accgcacccc tgggcccact cacgagtccc cccctcccca cctataaata
     8341 ccccaccccc tcctcgcctc ttcctccatc aatcgaatcc ccaaaatcgc agagaaaaaa
     8401 aaatctcccc tcgaagcgaa gcgtcgaatc gccttctcaa ggtatgcgat tttctgatcc
     8461 tctccgttcc tcgcgtttga tttgatttcc cggcctgttc gtgattgtga gatgttgttg
     8521 ttagtctccg ttttgcgatc tgtggtagat ttgaacaggg ttagatgggg ttcgcgtggt
     8581 atgctggatc tgtgattatg agcgatgctg ttcgtggtcc aagtatttat tggttcggat
     8641 ctagaagtag aactgtgcta gggttgtgat ttgttccgat ctgttcaatc agtaggattt
     8701 agtctctgtt tttctcgttg atccaagtag cagcttcagg tatattttgc ttaggttgtt
     8761 tttgattcag tccctctagt tgcatagatt ctactctgtt catgtttaat ctaagggctg
     8821 cgtcttgttg attagtgatt acatagcata gctttcagga tattttactt gcttatgcct
     8881 atcttatcaa ctgttgcacc tgtaaattct agcctatgtt aattaacctg ccttatgtgc
     8941 tctcgggata gtgctagtag ttattgaatc agtttgccga tggaattcta gtagttcata
     9001 gacctgcaga ttatttttgt gaactcgagc acggtgcgac tctctatttt gttaggtcac
     9061 tgttggtgtt gataggtaca ctgatgttat tgtggtttag atcgtgtatc taacatattg
     9121 gaataatttg attgactgat ttctgctgta cttgcttggt attgttataa tttcatgttc
     9181 atagttgctg accatgcttc ggtaattgtg tgtgcagcct gcaggaacaa agcaccagtg
     9241 gtctagtggt agaatagtac cctgccacgg tacagacccg ggttcgattc ccggctggtg
     9301 caggagacct tacagttgga cacaggccaa cttgtgaaga ttgcaaaacg tggcggcgtg
     9361 accgcaatgg aggcagtgca tgcatcgcgc aatgcactga cgggtgcccc cctggagacg
     9421 ggcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     9481 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     9541 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     9601 gcgcgcgtaa tacgactcac tatagggcga attgggtacc gggccccccc tcgaggtcct
     9661 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     9721 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     9781 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     9841 cactgcccgc tttccaccgg tggtctcagt ttgagtgtct tgttaagaaa ttaacaagat
     9901 gagttcaaat caggctccta gagagatccg aacttacctt catggcgggc attgtgccca
     9961 acaaagcacc agtggtctag tggtagaata gtaccctgcc acggtacaga cccgggttcg
    10021 attcccggct ggtgcaggta ctcgagccta gacttgtcca tcttctggat tggccaagtt
    10081 aattaatgta tgaaataaaa ggatgcacac atagtgacat gctaatcact ataatgtggg
    10141 catcaaagtt gtgtgttatg tgtaattact aattatctga ataagagaaa gagatcatcc
    10201 atatttctta tcctaaatga atgtcacgtg tctttataat tctttgatga accagatgca
    10261 ttttattaac caattccata tacatataaa tattaatcat atataattaa tatcaattgg
    10321 gttagcaaaa caaatctagt ctaggtgtgt tttgccgctg agctcccggg gcgcgccgat
    10381 atcgagctca gtgtggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    10441 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    10501 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc
    10561 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
    10621 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
    10681 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
    10741 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
    10801 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
    10861 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
    10921 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
    10981 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
    11041 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
    11101 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
    11161 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
    11221 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
    11281 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
    11341 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
    11401 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
    11461 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
    11521 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
    11581 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
    11641 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
    11701 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cggctcaacc gtgcggctgc
    11761 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
    11821 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
    11881 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
    11941 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
    12001 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
    12061 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
    12121 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
    12181 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
    12241 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
    12301 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
    12361 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
    12421 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
    12481 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
    12541 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
    12601 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
    12661 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
    12721 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
    12781 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
    12841 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
    12901 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc
    12961 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
    13021 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
    13081 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
    13141 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
    13201 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
    13261 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
    13321 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
    13381 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
    13441 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
    13501 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
    13561 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
    13621 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
    13681 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
    13741 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
    13801 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
    13861 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
    13921 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
    13981 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
    14041 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
    14101 gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac gtacattggg
    14161 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
    14221 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
    14281 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
    14341 ggccagcgca cagcccaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
    14401 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
    14461 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
    14521 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    14581 gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    14641 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
    14701 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
    14761 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggccc
    14821 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    14881 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    14941 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    15001 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    15061 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    15121 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    15181 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    15241 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    15301 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    15361 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    15421 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    15481 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    15541 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    15601 ttgatctttt ctacggggtc tgacgctcag tgctgagaga tcccctcata atttccccaa
    15661 agcgtaacca tgtgtgaata aattttgagc tagtagggtt gcagccacga gtaagtcttc
    15721 ccttgttatt gtgtagccag aatgccgcaa aacttccatg cctaagcgaa ctgttgagag
    15781 tacgtttcga tttctgactg tgttagcctg gaagtgcttg tcccaacctt gtttctgagc
    15841 atgaacgccc gcaagccaac atgttagttg aagcatcagg gcgattagca gcatgatatc
    15901 aaaacgctct gagctgctcg ttcggctatg gcgtaggcct agtccgtagg caggactttt
    15961 caagtctcgg aaggtttctt caatctgcat tcgcttcgaa tagatattaa caagttgttt
    16021 gggtgttcga atttcaacag gtaagttagt tgctagaatc catggctcct ttgccgacgc
    16081 tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt gtcgagcgct gattttttcg
    16141 gcctttagag cgagatttat acaatagaat ttggcatgag attggattgc ttttagtcag
    16201 cctcttatag cctaaagtct ttgagtgact agatgacata tcatgtaagt tgctgatagg
    16261 tttccagttt tccgctccta ggtctgcata ttgtactttt cctcttactc gacttaacca
    16321 gtaccaaccc agcttctcaa cggatttata ccatggcact ttaaagccag catcactgac
    16381 aatgagcggt gtggtgttac tcggtagaat gctcgcaagg tcggctagaa attggtcatg
    16441 agctttcttt gaacattgct ctgaaagcgg gaacgctttc tcataaagag taacagaacg
    16501 accgtgtagt gcgactgaag ctcgcaatac cataagccgt ttttgctcac ggatatcaga
    16561 ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag ataaagctag catgccaacg
    16621 gtatacagcg agtcgctctt tgtggaggtg acgattacct aacaatcggt cgattcgttt
    16681 gatgttatgt tttgttctcg ctttggttgg caggttacgg ccaagttcgg taagagtgag
    16741 agttttacag tcaagtaagg cgtggcaagc caacgttaag ctgttgagtc gttttaagtg
    16801 taattcgggg cagaattggt aaagagagtc gtgtaaaata tcgagttcgc acattttgtt
    16861 gtctgattat tgatttttgg cgaaaccatt tgatcatatg acaagatgtg tatctacctt
    16921 aacttaatga ttttgataaa aatcattagg ggattcatca gcgctcagtg gaacgaaaac
    16981 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
    17041 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
    17101 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
    17161 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
    17221 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
    17281 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
    17341 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
    17401 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
    17461 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
    17521 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
    17581 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
    17641 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
    17701 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
    17761 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
    17821 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    17881 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    17941 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
    18001 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
    18061 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
    18121 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
    18181 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
    18241 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
    18301 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt
    18361 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
    18421 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
    18481 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
    18541 cggcatctac tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
    18601 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
    18661 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
    18721 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
    18781 atatacgccc ggagtcgtgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
    18841 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
    18901 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
    18961 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
    19021 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
    19081 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
    19141 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
    19201 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
    19261 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
    19321 ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
    19381 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
    19441 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
    19501 atcaggtcgg acacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
    19561 agttcaggct ttttcatatc tcattgcccc ccggatctgc gaaagctcga gagagataga
    19621 tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat
    19681 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
    19741 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
    19801 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata
    19861 gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt
    19921 ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt
    19981 tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag
    20041 tagacgagag tgtcgtgctc caccatgttc acatcaatcc acttgctttg aagacgtggt
    20101 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
    20161 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
    20221 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
    20281 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
    20341 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttggcaag
    20401 ctgctctagc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    20461 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    20521 tagctcactc attaggcacc ccaggcttta cactttatgc ttccrgckcr wmkswwktrw
    20581 kg
//
