LOCUS       sequence-430343-        9400 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of PlsB, PlsC, CdsA and PssA under the control of T7 
            promoters. The 4-gene insert is flanked by Phi29 replication 
            origins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   van den Brink M, Althuis TY, Danelon C, Claassens NJ
  TITLE     MOSAIC: A Highly Efficient, One-Step Recombineering Approach to 
            Plasmid Editing and Diversification.
  JOURNAL   ACS Synth Biol. 2025 Jun 5. doi: 10.1021/acssynbio.4c00657.
  PUBMED    40471122
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 Jun 5. doi: 10.1021/acssynbio.4c00657."
COMMENT     Sequence Label: G555
FEATURES             Location/Qualifiers
     source          1..9400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6..52
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(309..325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(309..325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(322..344)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    333..349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(357..363,364..381,382..387))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    402..423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(540..557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(711..1299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(791..810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1470..2261,2262..2330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2093..2112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2331..2435)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2503..2521
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2559..2581)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2681..2700
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2894..2916
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2908..2925
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2909..2925
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3188..3206
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3238..3260
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      5820..5867
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5873..5891
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             5923..5945
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      6759..6806
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6812..6830
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6831..6855
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6870..6892
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6900..6932
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     terminator      7855..7902
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        7908..7926
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7927..7951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7966..7988
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7996..8028
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tcgagtagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgcctcctat
       61 gattggttgt cttattacct tacttctatt atagtataac atgttaaacg atagtttgtc
      121 tacccttttc gacaaattga tgataataaa tagtataggt atatagtcgt gatttagttg
      181 ttagattctt gtcgaagata gtcggtcaat ggggaaatgg tgtatgttgt cgctgtaccc
      241 tactttattg gatcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggcg
      301 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      361 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      421 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      481 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2461 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2521 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     2581 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     2641 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     2701 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2761 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     2821 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     2881 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt
     2941 acctcgcgaa tgcatctaga tccaataaag taagccccca ccctcacatg ataccattct
     3001 cctaatatcg acataatccg tcgatcctcg gcataccatg atcagggagg gaaactacta
     3061 cttaatatat caatctatag acctactaga taggtttgtc aatgaacaac ataaaacgac
     3121 acagaatccc acgttttagc gcttcgtctg tgtcgcatgt ccatggctag aacgtctcaa
     3181 tctctattaa tacgactcac tatagggtca ccacaacggt ttccctctag aaataatttt
     3241 gtttaacttt aagaaggaga tatacatatg actttctgct atccttgccg cgcatttgca
     3301 ttattaacca gaggctttac atcgtttatg tccggctggc cacgaattta ctacaaatta
     3361 ctgaatttac cattaagcat cctggtaaaa agcaagtcta ttccggcaga tcctgccccg
     3421 gaactggggc tggatacctc tcgtccaatt atgtacgttt taccgtacaa ctcgaaagca
     3481 gatttgctga cgttacgcgc ccagtgtctg gctcatgact tgcctgaccc gttagagccg
     3541 ctggaaatcg acggcacgct actgccgcgc tatgtgttca ttcacggcgg gccgcgtgta
     3601 ttcacctatt acacgccgaa agaagagtct attaagctgt tccacgacta tctcgatttg
     3661 caccgtagca acccaaatct ggatgtgcag atggtgccag tatcggtgat gtttggtcgc
     3721 gcgccggggc gtgaaaaagg cgaagtaaac ccgccgctgc gtatgcttaa cggcgtacag
     3781 aaatttttcg ccgtactgtg gctcggtcgc gacagttttg tgcgtttctc gccgtcagtt
     3841 tcgctgcgcc gtatggcgga tgaacacggc acggataaaa ctatcgcaca gaaactggcg
     3901 cgcgtggcgc gtatgcactt tgcccgtcaa cgtctggctg ctgtgggccc acgtctgcct
     3961 gctcgtcagg atctgtttaa taagctgctc gcctcccgcg ccattgccaa agcggtagaa
     4021 gatgaagcgc gcagcaaaaa aatctcccat gaaaaagcgc agcagaacgc gattgcgctg
     4081 atggaagaga ttgcggcgaa tttctcttac gagatgattc gcctgactga ccgtattctg
     4141 ggcttcacct ggaaccgcct ttaccagggc atcaacgtcc ataacgccga gcgcgttcgc
     4201 cagctggccc acgacggtca tgagctggta tatgtgcctt gccaccgcag ccacatggac
     4261 tacctgctgc tttcttacgt gctgtatcac caggggctgg tgccgccgca tatcgccgct
     4321 gggatcaacc tgaacttctg gccggccggg ccgattttcc gccgtctggg ggcgttcttt
     4381 attcgccgta cgttcaaagg caataaactt tattccaccg ttttccgtga gtatctcggc
     4441 gaactgttca gccgtggtta ttccgtcgag tacttcgtgg aaggcggtcg ttcccgcacg
     4501 gggcgtttgc tggatccgaa aaccggtacg ctgtcgatga ccattcaggc gatgctgcgt
     4561 ggcggcacgc gtccgattac gctgattccg atctacatcg gttatgagca cgtcatggaa
     4621 gtgggtactt acgccaaaga actgcgcggc gcgacgaaag agaaagagag cctgccgcag
     4681 atgctgcgcg gtttaagcaa gctgcgtaat ctcggtcagg gttacgtcaa cttcggtgaa
     4741 ccaatgccgt tgatgaccta ccttaaccag cacgtaccag actggcgtga atctatcgat
     4801 cccatcgaag cggtgcgtcc ggcatggtta acgccgacgg tcaataatat tgctgccgat
     4861 ctgatggtac gcattaacaa cgcaggcgcg gcaaacgcca tgaacctgtg ctgtactgcg
     4921 cttctggcgt cacgtcagcg ctcactcacc cgcgagcagt taaccgagca actcaactgc
     4981 tacctggatc tgatgcgcaa cgtaccttac tccacggact ctaccgttcc ttcagccagc
     5041 gccagcgagc ttatcgatca cgcgctgcaa atgaacaagt ttgaagtcga gaaagacaca
     5101 atcggcgaca tcatcattct gccgcgcgag caagcggtgc tgatgaccta ctatcgcaac
     5161 aacattgcgc atatgttggt gctgccttcg ctgatggcgg caatcgtcac ccagcatcgc
     5221 cacatctccc gcgacgtatt gatggagcac gtcaatgtgc tttacccaat gctgaaagcg
     5281 gagctgttcc tgcgctggga tcgcgacgag ttgccggacg ttattgatgc gctggcaaat
     5341 gagatgcaac gtcaggggct gattaccctg caagatgatg agttgcatat caacccggcg
     5401 cattctcgca cgctacagct gctggccgca ggcgcgcgcg aaacgctgca acgttatgcc
     5461 atcaccttct ggttgttgag tgccaacccg tcgatcaacc gcggtacgct ggagaaagag
     5521 agccgcaccg tcgcgcaacg tctctccgtg ctgcacggca tcaacgcgcc ggagttcttc
     5581 gacaaggcgg tgttcagttc tctggtgctg acactgcgtg atgaagggta tatcagcgat
     5641 agcggcgatg ccgaaccggc agaaacgatg aaggtttatc agttgctggc ggagttgatt
     5701 acatcagacg tgcgtttgac gattgagagt gcgacgcagg gcgaagggta agcttggatc
     5761 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
     5821 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgcgc aataatacga
     5881 ctcactatag ggtcaccaca acggtttccc tctagaaata attttgttta actttaagaa
     5941 ggagatatac atatgctata tatctttcgt cttattatta ccgtgattta cagcatctta
     6001 gtctgtgtat tcggctccat ttactgcctt ttcagcccgc gtaacccgaa acatgtggcc
     6061 acctttgggc atatgtttgg ccgtcttgcg ccgctgtttg gcctgaaagt tgagtgccgt
     6121 aaacctacag acgctgaaag ctacggcaat gctatctata tcgctaacca ccagaacaac
     6181 tatgacatgg tgacagcatc gaacatcgtg caaccgccga cggtgacggt aggtaaaaag
     6241 agcttgctgt ggatcccctt cttcgggcag ttgtactggt taaccggcaa cttattgatc
     6301 gacagaaaca atcgcactaa agctcacggc accattgcgg aagtagtgaa tcacttcaaa
     6361 aaacgccgta tttccatctg gatgttcccg gaaggaaccc gcagccgtgg tcgcggcctg
     6421 ctaccgttca agactggagc atttcacgcg gcaattgcgg cgggcgtccc gattattccc
     6481 gtgtgcgtct ctacaacttc gaataagatt aatcttaatc gactgcacaa cggtctggtg
     6541 attgtcgaaa tgctgccgcc aattgacgtc agtcagtatg gcaaagatca ggttcgtgag
     6601 ctggctgccc attgtcgttc gataatggaa caaaaaatcg ccgagctcga taaagaagtc
     6661 gcagaacgcg aagccgccgg aaaagtttaa gcttggatcc ggctgctaac aaagcccgaa
     6721 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
     6781 ctaaacgggt cttgaggggt tttttgcact ataatacgac tcactatagg ggaattgtga
     6841 gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata
     6901 tggctagcat gactggtgga cagcaaatgg gtcgcggatc cggctgcttg ctgaagtatc
     6961 gcctgatatc tgcttttgtg ttaatacccg tcgtcatcgc ggcgttgttt ctgttgccgc
     7021 cggtggggtt cgccattgta acgctggtgg tctgcatgct ggcagcgtgg gaatggggac
     7081 agcttagcgg ttttaccact cgttcgcagc gagtatggtt ggcggtgtta tgcgggttat
     7141 tgttggcgct gatgcttttt ctgttgccgg aatatcaccg aaatattcat caaccgctgg
     7201 ttgaaatctc actttgggct tcgctgggtt ggtggattgt cgcgctattg ctggtgctgt
     7261 tttacccagg ttccgcagca atctggcgta actctaaaac attgcgcctt atttttggcg
     7321 tgctaaccat tgttcccttc ttctggggca tgctggcgtt acgggcctgg cactatgacg
     7381 agaatcatta cagtggcgca atatggctgc tctatgtcat gatcctggta tggggcgctg
     7441 actccggcgc atatatgttt ggcaaattgt ttggtaaaca taagctggca ccgaaggttt
     7501 ctccgggtaa aacctggcaa ggctttatcg gtggactcgc tactgcagcg gtaatctcat
     7561 ggggttatgg catgtgggcg aatctcgacg tcgctcccgt caccttactc atttgctcta
     7621 ttgtcgcagc gttagcctca gtgctcggcg atctgaccga gagtatgttt aagcgtgaag
     7681 caggaattaa ggacagcggt catttaattc caggacacgg tggtatttta gatcgtattg
     7741 atagcctgac ggctgcggta ccggtctttg cttgcttgtt gttactggta ttcaggacgc
     7801 tttaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     7861 taaccccttg gggcctctaa acgggtcttg aggggttttt tgcttactaa tacgactcac
     7921 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
     7981 gaaggagata tacatatggc tagcatgact ggtggacagc aaatgggtcg cggatccggc
     8041 tgcatgttgt caaaatttaa gcgtaataaa catcaacaac accttgccca actacccaag
     8101 atttctcaat cagttgatga tgtcgatttc ttttacgctc ccgccgactt ccgggagacg
     8161 ctgctggaaa aaatagccag cgcgaagcag cgcatttgca ttgtcgccct gtatctcgaa
     8221 caggatgacg gtggcaaagg cattctgaac gcgttgtatg aggctaaaag gcagcgtccg
     8281 gaactggatg tgcgggtgct ggtcgactgg catcgtgcac aacgtggacg cattggcgct
     8341 gcggcatcta acactaacgc tgactggtac tgccgcatgg cgcaggaaaa tccgggcgta
     8401 gatgttccgg tttatggcgt tccaatcaat actcgtgaag cccttggtgt tctgcacttt
     8461 aaaggcttta tcatcgacga tagcgtactt tatagcggtg ccagcctgaa cgatgtttac
     8521 ctgcatcagc acgataaata tcgctacgac cgttatcatc tgatccgtaa ccgtaagatg
     8581 tcagacatta tgtttgaatg ggttacacag aatattatga atggccgcgg cgttaatcgt
     8641 ctggatgatg ttaatcggcc aaaaagcccg gaaatcaaga acgatattcg tctgttccgc
     8701 caggagctgc gtgatgccgc ttatcatttc cagggcgatg ccgacaacga tcagctttct
     8761 gtaacgccgc tagtggggct ggggaaatcg agtctgttga acaagaccat tttccatctt
     8821 atgccttgtg ccgagcagaa actaaccatc tgtacgccat acttcaacct gccagcaatc
     8881 cttgtgcgca atattatcca gttgctgcgc gaagggaaaa aggtcgaaat tattgttggt
     8941 gataaaaccg cgaatgactt ctacattccg gaagatgaac ctttcaagat aattggcgca
     9001 ttgccttatc tctatgagat caatctgcgt cgtttcctga gccgtttgca gtattacgtc
     9061 aatactgacc agctagtggt tcggttatgg aaagatgacg acaacaccta tcacctgaaa
     9121 gggatgtggg ttgatgataa gtggatgttg atcaccggta ataacctgaa cccgcgcgcc
     9181 tggcgtctgg atctggaaaa cgccattttg atccacgatc cgcaacttga gctggcgcca
     9241 cagcgagaga aagaactgga gctgatccgc gagcatacca ccatcgttaa gcactatcgc
     9301 gatctgcaaa gtattgccga ttatccggtg aaggttcgta aactcatccg ccgtttgcgc
     9361 cgtatccgca tcgaccgatt aattagccgc atcctgtaac
//
