LOCUS       sequence-430345-       19001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FrCas9 adenine base editing plasmid in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19001)
  AUTHORS   He Y, Han Y, Ma Y, Liu S, Fan T, Liang Y, Tang X, Zheng X, Wu Y, 
            Zhang T, Qi Y, Zhang Y
  TITLE     Expanding plant genome editing scope and profiles with CRISPR-FrCas9
            systems targeting palindromic TA sites.
  JOURNAL   Plant Biotechnol J. 2024 Sep;22(9):2488-2503. doi: 
            10.1111/pbi.14363. Epub 2024 May 7.
  PUBMED    38713743
REFERENCE   2  (bases 1 to 19001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14363";
            journalName: "Plant Biotechnol J"; date: "2024-09"; volume: "22"; 
            issue: "9"; pages: "2488-2503"
COMMENT     Sequence Label: pGEL849
FEATURES             Location/Qualifiers
     source          1..19001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    13..34
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(49..54,55..72,73..79)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    87..103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     111..127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2166..2175
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2178..2198
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2823..2888
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2895..2915
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7053..7100
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      7119..7367
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(9013..9420)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     9285..9301
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9311..9330
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9311..9329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9377..9397)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9377..9395)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9416..9432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9440..9456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9464..9470,9471..9488,9489..9494))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9509..9530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10101..10117)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10320..10344
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11645..12274
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12703..13776
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13842..14036
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14272..14294)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14380..14520
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14435..14454
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14535..14552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14706..15294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14786..14805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15381..16175)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    16600..16624
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    16702..16876
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(16917..17942)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             complement(16917..17942)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(18015..18360)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(18052..18072)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     gap             18561..18660
                     /estimated_length=100
     protein_bind    18904..18925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(18940..18945,18946..18963,18964..18970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18978..18994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
       61 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
      121 ctatgacatg attacgaatt cgagctcggt acccggggat cggcgcgcct gcagtgcagc
      181 gtgacccggt cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa
      241 ttaccacata ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata
      301 tatttaaact ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt
      361 agagaatcat ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa
      421 caggactcta cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag
      481 cttcacctat ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa
      541 tggtttttat agactaattt ttttagtaca tctattttat tctattttag cctctaaatt
      601 aagaaaacta aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata
      661 aaataaagtg actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac
      721 atttttcttg tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg
      781 acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc
      841 tctgtcgctg cctctggacc cctctcgaga gttccgctcc accgttggac ttgctccgct
      901 gtcggcatcc agaaattgcg tggcggagcg gcagacgtga gccggcacgg caggcggcct
      961 cctcctcctc tcacggcacc ggcagctacg ggggattcct ttcccaccgc tccttcgctt
     1021 tcccttcctc gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc
     1081 gtgttgttcg gagcgcacac acacacaacc agatctcccc caaatccacc cgtcggcacc
     1141 tccgcttcaa ggtacgccgc tcgtcctccc ccccccccct ctctaccttc tctagatcgg
     1201 cgttccggtc catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc
     1261 gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac
     1321 acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc
     1381 gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg
     1441 cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct
     1501 tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag
     1561 aattaattct gtttcaaact acctggtgga tttattaatt ttggatctgt atgtgtgtgc
     1621 catacatatt catagttacg aattgaagat gatggatgga aatatcgatc taggataggt
     1681 atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt tcgcttggtt
     1741 gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt agaatactgt
     1801 ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca tacatcttca
     1861 tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca tgttgatgtg
     1921 ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg ctctaacctt
     1981 gagtacctat ctattataat aaacaagtat gttttataat tattttgatc ttgatatact
     2041 tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct tcatacgcta
     2101 tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg tgttacttcc
     2161 tgcaggccgc catggctcct aagaagaagc ggaaggttgg tattcacggg gtgcctgcgg
     2221 cttctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga
     2281 gggcacggga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga
     2341 tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta
     2401 tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac gccaccctgt
     2461 acgtgacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct aggatcggcc
     2521 gcgtggtgtt tggcgtgagg aactcaaaaa gaggcgccgc aggctccctg atgaacgtgc
     2581 tgaactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg gcagatgaat
     2641 gtgccgccct gctgtgcgat ttctatcgga tgcctagaca ggtgttcaat gctcagaaga
     2701 aggcccagag ctccatcaac tccggaggat ctagcggagg ctcctctggc tctgagacac
     2761 ctggcacaag cgagagcgca acacctgaaa gcagcggggg cagcagcggg gggtcaacca
     2821 tggattacaa ggaccacgac ggggattaca aggaccacga cattgattac aaggatgatg
     2881 atgacaagat ggctccgaag aagaagagga aggttggcat ccacggggtg ccagctgctt
     2941 gcaccaagga gtcggaaaag ctcaataaga acgcggatta ctacattggt ctggacatgg
     3001 gcacatcctc agctggctgg gcggtgagtg actccgagta caatctaatc cgcagaaaag
     3061 gcaaggattt gtggggcgtt cgccagttcg aagaagcgaa aacggcggcg gaacgccggg
     3121 ggttccgggt cgctcgtcga aggaaacaga ggcagcaggt caggaatcga ctattgagtg
     3181 aggaatttca gaatgaaatc acaaagatcg attctgggtt tttaaaaaga atggaggatt
     3241 ctcggtttgt tattagcgac aagagggtgc cagagaagta caccctgttc aatgattccg
     3301 ggtacacaga tgtagaatac tacaaccagt acccgaccat ctaccatctg agaaaagcct
     3361 taatcgaatc caatgagagg tttgatattc gccttgtatt cctgggaatc cactcgttgt
     3421 tccagcatcc aggccatttc ctcgacaagg gggacgtgga tacggacaat acaggtcccg
     3481 aagaactgat acagtttctc gaagactgca tgaacgagat acaaatcagc atcccgctcg
     3541 tctcaaacca aaaagtctta actgatatct tgacggatag ccgaataacg aggcgtgaca
     3601 aagaacagca aattcttgag atattgcaac ccaacaaaga gtccaaaaag gccgtttccc
     3661 agttcgtgaa ggttctcaca ggtcaaaaag ccaagttagg cgatctcatt atgatggaag
     3721 ataaagatac ggaggagtac aagtattcat tttctttccg cgaaaagacc ctggaggaga
     3781 ttcttcccga tatagagggc gtcatcgacg gtcttgcgct tgagtatatc gaatctatct
     3841 actcgctata ttcatggagc ctcctcaaca gctacatgaa agatacattg accggtcatt
     3901 attactcgta tctagctgag gcccgcgtgg cggcgtacga taagcatcat agtgatcttg
     3961 ttaagctgaa aacattgttc cgcgagtata tcccggaaga atacgacaac ttcttccgca
     4021 agatggagaa ggccaactat tcgcattata ttggcagcac cgagtatgat ggtgagaagc
     4081 gttgtagaac agccaaagcc aagcaagagg acttctacaa gagtatcaac aaaatgctcg
     4141 aaaagatccc tgagtgcagt gaaaaaacgg agatccagaa ggagataata gagggcactt
     4201 tcctccttaa gcaaactggg cctcagaacg gctttgtccc gaatcagtta cagctaaaag
     4261 aactacgaaa gatcctgcaa aatgcgtcca agcactaccc ctttctaaca gagaaggatg
     4321 aaagagatat gactgccatt gacagaatcg aggcgctgtt cagctttaga atcccgtact
     4381 acatcggccc actcaagaac acggacaatc aagggcacgg ttgggctgtt aggagggacg
     4441 ggcacgagca aatcccggta cggccgtgga acttcgaaga aatcattgat gagtccgcta
     4501 gcgccgatct gttcatcaag aatttggtga acagttgtac ttacctcagg accgagaagg
     4561 tactcccaaa atctagcttg ctgtatcaag aattcgaagt cctgaatgag ctcaacaacc
     4621 tcaggataaa tgggatgtac ccagatgaaa ttcagccagg cctaaagcgt atgatcttcg
     4681 agcagtgctt ctattctggc aaaaaagtca ctggaaaaaa actgcagtta ttcctcagga
     4741 gtgtgctcac caattcttca acggaggagt ttgttctaac aggcattgac aaggacttta
     4801 aatcgtccct gtcgagttac aaaaaattct gcgagctttt tggcgttaag acactgaacg
     4861 atacacagaa ggtgatggct gaacagatta ttgaatggtc cactgtctat ggcgattcgc
     4921 gcaagtttct taagagaaag ctcgaggata actatccgga acttacagat caacaaattc
     4981 gacggattgc tggcttcaag ttctccgagt gggggaacct gagcagggca ttcctcgaga
     5041 tggaggggta caaggacgaa gcaggaaatc ctgttaccat aattagagcg cttcgtgata
     5101 ctcaaaaaaa cttgatgcag ctcctctcca acgactcagc attcgctaag aagctgcagg
     5161 agttaaatga ctacgtgacg cgcgacatct ggtcaataga gccggacgac ctcgatggaa
     5221 tgtatctgtc tgctcctgtt cgccggatga tatggcagac atttttgatc ctcagagaag
     5281 tggtcgatac cattggttat tcacctaaga agattttcat ggcgatggcg cggggggagc
     5341 aggagaagaa gaggactgcg tctaggaaga aacagttgat agatctctac aaggaggcag
     5401 gcatgaagaa cgatgaattg tttggagatc tcgagagcct ggaagaggcc caattgaggt
     5461 ccaagaagct ctatctttat ttccggcaga tggggaggga tatttatagt ggaaagctaa
     5521 ttgatttcat ggatgtactc cacggcaatc ggtacgacat agaccacatc cacccgcaaa
     5581 gcaagaagaa agatgactcc ttagaaaaca atctggtact cacaagcaag gacttcaata
     5641 atcatattaa acaagacgtc taccccattc ctgagcaaat tcaatcgaga caaaaaggat
     5701 tttgggccat gctcctgaag caaggtttca tgtctcagga gaaatacaac cgactcatgc
     5761 ggacgactcc gttcaccgat gaggagctgg cggagttcgt caacagacaa ctggtggaga
     5821 ctagacaggg aacaaaagca attatatcac tcatcaacca gtgtttccct gactcagaag
     5881 ttgtgtacgt gaaggccgga aacacctcgg acttccggca gcggttcgac attccaaagt
     5941 cgcgggactt gaacaattac caccacgccg tagacgccta tcttaacatc gttgtcggca
     6001 acgtttatga caccaaattt accaaaaatc caatcaattt tattaagaag atgaggaaat
     6061 ctggtaattt gcactcctat agtttaagaa ggatgtatga tttcaatgtc cagcgcggag
     6121 atcagaccgc gtgggttgcc gaaaatgaca cgacgttaaa aacagtgaag aagacagctt
     6181 ttaagacctc gcccatggtg acaaaacgga cgtacgagag gaaaggtggg ttggcagatt
     6241 ccgtcctgat agccgccaag aaggcaaagc ctggggtcca tcttccagtg aaaacttcag
     6301 actcacgctt tgcaaaccag gtgtccacct acggcggcta cgacaacgtg aagggaagcc
     6361 acttctttct tgttgagcat cagcagaaga aaaaaacaat aaggagcata gagaatgtcc
     6421 cgatccatct taaagagaag ttaaagacca aggaggaact cgaacactac tgtgcacaag
     6481 tgctcggcat ggtgcagcct gatgtccgtc tgactcgcat cccaatgtac tctcttctat
     6541 tgattgatgg ctattactat tacctgaccg gacgtacggg tggcaatctg tctctctcta
     6601 atgcggttga gctctgcttg ccagccaagg agcaggcaca tatcaggatg atttcaaaaa
     6661 tagccggggg gcgctcaacg gacgctttgt ctgcggaggc caaggacgat tttcgcaaga
     6721 aaaatcttcg gctctatgac gaactcgctg aaaagcaccg gtcaactatt ttttcgaaac
     6781 gcaagaaccc catcggaccc aagctgctga agtacagaga ggcatttgtg aaacaaacta
     6841 tcgagaatca atgcaaggtt attttacaga tattgaagtt gaccagcaca aactgcaaaa
     6901 cttctgctga ccttaagctt atcggaggtt cagggcaaga aggcgtcatg agtatttcca
     6961 agctcctacg agctgaaaag tatgcagaat tttacctaat ttgtcagtcc cctagcggaa
     7021 tttatgagac tcgaaagaac ctgctgacta taaagcgtcc tgctgccacc aaaaaggccg
     7081 gacaggctaa gaaaaagaag tgaacgcgta ctcgagtata tgaagatgaa gatgaaatat
     7141 ttggtgtgtc aaataaaaag cttgtgtgct taagtttgtg tttttttctt ggcttgttgt
     7201 gttatgaatt tgtggctttt tctaatatta aatgaatgta agatcacatt ataatgaata
     7261 aacaaatgtt tctataatcc attgtgaatg ttttgttgga tctcttctgc agcatataac
     7321 tactgtatgt gctatggtat ggactatgga atatgattaa agataaggag ctcggacggc
     7381 gcgccggagc acatcagtct ctgcacaaag tgcatcctgg gctgcttcaa ttataaagcc
     7441 ccattcacca catttgctag atagtcgaaa agcaccatca atattgagct tcaggtattt
     7501 ttggttgtgt tgtggttgga ttgattctaa tatataccaa atcaatataa ttcactacca
     7561 aaatatacca tagccatcac aactttatta attttggtag cttaagatgg tatatataat
     7621 aaccaattaa caactgattc taattttact acggcccagt atctaccaat acaaaacaac
     7681 gagtatgttt tcttccgtcg taatcgtaca cagtacaaaa aaacctggcc agcctttctt
     7741 gggctggggc tctctttcga aaggtcacaa aacgtacacg gcagtaacgc cgcttcgctg
     7801 cgtgttaacg gccaccaacc ccgccgtgag caaacggcat cagctttcca cctcctcgat
     7861 atctccgcgg cgccgtctgg acccgccccc tttccgttcc tttctttcct tctcgcgttt
     7921 gcgtggtggg gacggactcc ccaaaccgcc tctccctctc tttatttgtc tatattctca
     7981 ctgggcccca cccaccgcac ccctgggccc actcacgagt ccccccctcc ccacctataa
     8041 ataccccacc ccctcctcgc ctcttcctcc atcaatcgaa tccccaaaat cgcagagaaa
     8101 aaaaaatctc ccctcgaagc gaagcgtcga atcgccttct caaggtatgc gattttctga
     8161 tcctctccgt tcctcgcgtt tgatttgatt tcccggcctg ttcgtgattg tgagatgttg
     8221 ttgttagtct ccgttttgcg atctgtggta gatttgaaca gggttagatg gggttcgcgt
     8281 ggtatgctgg atctgtgatt atgagcgatg ctgttcgtgg tccaagtatt tattggttcg
     8341 gatctagaag tagaactgtg ctagggttgt gatttgttcc gatctgttca atcagtagga
     8401 tttagtctct gtttttctcg ttgatccaag tagcagcttc aggtatattt tgcttaggtt
     8461 gtttttgatt cagtccctct agttgcatag attctactct gttcatgttt aatctaaggg
     8521 ctgcgtcttg ttgattagtg attacatagc atagctttca ggatatttta cttgcttatg
     8581 cctatcttat caactgttgc acctgtaaat tctagcctat gttaattaac ctgccttatg
     8641 tgctctcggg atagtgctag tagttattga atcagtttgc cgatggaatt ctagtagttc
     8701 atagacctgc agattatttt tgtgaactcg agcacggtgc gactctctat tttgttaggt
     8761 cactgttggt gttgataggt acactgatgt tattgtggtt tagatcgtgt atctaacata
     8821 ttggaataat ttgattgact gatttctgct gtacttgctt ggtattgtta taatttcatg
     8881 ttcatagttg ctgaccatgc ttcggtaatt gtgtgtgcag cctgcaggaa caaagcacca
     8941 gtggtctagt ggtagaatag taccctgcca cggtacagac ccgggttcga ttcccggctg
     9001 gtgcaggaga ccttacagtt ggacacaggc caacttgtga agattgcaaa acgtggcggc
     9061 gtgaccgcaa tggaggcagt gcatgcatcg cgcaatgcac tgacgggtgc ccccctggag
     9121 acgggcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     9181 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     9241 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     9301 tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt
     9361 cctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     9421 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9481 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9541 gctcactgcc cgctttccac cggtggtctc agtttgagtg tcttgttaag aaattaacaa
     9601 gatgagttca aatcaggctc ctagagagat ccgaacttac cttcatggcg ggcattgtgc
     9661 ccaacaaagc accagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt
     9721 tcgattcccg gctggtgcag gtactcgagc ctagacttgt ccatcttctg gattggccaa
     9781 gttaattaat gtatgaaata aaaggatgca cacatagtga catgctaatc actataatgt
     9841 gggcatcaaa gttgtgtgtt atgtgtaatt actaattatc tgaataagag aaagagatca
     9901 tccatatttc ttatcctaaa tgaatgtcac gtgtctttat aattctttga tgaaccagat
     9961 gcattttatt aaccaattcc atatacatat aaatattaat catatataat taatatcaat
    10021 tgggttagca aaacaaatct agtctaggtg tgttttgccg ctgagctccc ggggcgcgcc
    10081 gatatcgagc tcagtgtggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    10141 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    10201 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag
    10261 agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt
    10321 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
    10381 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
    10441 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
    10501 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
    10561 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
    10621 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
    10681 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
    10741 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
    10801 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
    10861 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
    10921 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
    10981 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
    11041 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
    11101 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
    11161 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
    11221 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
    11281 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
    11341 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
    11401 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacggctca accgtgcggc
    11461 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
    11521 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
    11581 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
    11641 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
    11701 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
    11761 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
    11821 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
    11881 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
    11941 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
    12001 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
    12061 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
    12121 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
    12181 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
    12241 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
    12301 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
    12361 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
    12421 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
    12481 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
    12541 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
    12601 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg
    12661 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
    12721 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
    12781 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
    12841 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
    12901 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
    12961 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
    13021 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
    13081 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
    13141 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
    13201 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
    13261 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
    13321 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
    13381 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
    13441 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
    13501 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
    13561 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
    13621 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
    13681 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
    13741 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
    13801 attgccctag caggggaaaa aggtcgaaaa ggcctctttc ctgtggatag cacgtacatt
    13861 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
    13921 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
    13981 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
    14041 tctggccagc gcacagccca agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
    14101 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
    14161 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
    14221 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    14281 catgcagctc ccggaaacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    14341 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
    14401 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
    14461 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
    14521 ccctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    14581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    14641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    14701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    14761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    14821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    14881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    14941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    15001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    15061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    15121 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
    15181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    15241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    15301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    15361 ttggtcatgc attctaggta ctaaaacaat tcatccagta aaatataata ttttattttc
    15421 tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg
    15481 atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg
    15541 cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct
    15601 cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca
    15661 tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg
    15721 gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta
    15781 tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg
    15841 ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc
    15901 tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca
    15961 ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc
    16021 cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta
    16081 tataccttag caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt
    16141 tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac
    16201 agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc
    16261 ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg
    16321 cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc
    16381 tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt tcaaacccgg
    16441 cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct gccgccttac
    16501 aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga gtggtgattt
    16561 tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat attgtggtgt
    16621 aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa tgtactgaat
    16681 taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt ttaggaatta
    16741 gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt ttcttatatg
    16801 ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga actactcaca
    16861 cattattatg gagaaactcg agcttgtcga tcgacagatc ccggtcggca tctactctat
    16921 ttctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
    16981 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
    17041 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
    17101 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagt
    17161 cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
    17221 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
    17281 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
    17341 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
    17401 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
    17461 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
    17521 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
    17581 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
    17641 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
    17701 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
    17761 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
    17821 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggacacg
    17881 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
    17941 atatctcatt gccccccgga tctgcgaaag ctcgagagag atagatttgt agagagagac
    18001 tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
    18061 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
    18121 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
    18181 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
    18241 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
    18301 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
    18361 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
    18421 tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
    18481 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
    18541 ttgaacgata gcctttcctt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
    18721 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
    18781 ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat
    18841 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    18901 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    18961 tcgtatgttg tgtggaattg tgagcggata acaatttcac a
//
