LOCUS       sequence-430354-       20426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TREX2-FrCas9 genome editing in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20426)
  AUTHORS   He Y, Han Y, Ma Y, Liu S, Fan T, Liang Y, Tang X, Zheng X, Wu Y, 
            Zhang T, Qi Y, Zhang Y
  TITLE     Expanding plant genome editing scope and profiles with CRISPR-FrCas9
            systems targeting palindromic TA sites.
  JOURNAL   Plant Biotechnol J. 2024 Sep;22(9):2488-2503. doi: 
            10.1111/pbi.14363. Epub 2024 May 7.
  PUBMED    38713743
REFERENCE   2  (bases 1 to 20426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14363";
            journalName: "Plant Biotechnol J"; date: "2024-09"; volume: "22"; 
            issue: "9"; pages: "2488-2503"
COMMENT     Sequence Label: pGEL847
FEATURES             Location/Qualifiers
     source          1..20426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    191..215
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1516..2145
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2574..3647
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3713..3907
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4143..4165)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4251..4391
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4306..4325
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4406..4423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4577..5165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4657..4676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6590..7384)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7809..7833
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7911..8085
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8126..9151)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             complement(8126..9151)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9224..9569)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9261..9281)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     gap             9770..9869
                     /estimated_length=100
     protein_bind    10113..10134
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10149..10154,10155..10172,10173..10179)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10187..10203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10211..10227
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10274..12271
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             12287..12307
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13094..13114
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             17252..17299
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      17315..17563
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(19213..19620)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     19485..19501
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     19511..19530
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        19511..19529
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(19577..19597)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(19577..19595)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(19616..19632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    19640..19656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(19664..19670,19671..19688,19689..19694))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    19709..19730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(20301..20317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
       61 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      121 gcgaatgcta gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac
      181 tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga
      241 ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
      301 gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata
      361 gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt
      421 cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt
      481 ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga
      541 gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca
      601 accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg
      661 gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg
      721 ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg
      781 ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca
      841 ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc
      901 gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga
      961 agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg
     1021 accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc
     1081 tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg
     1141 ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc
     1201 aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga
     1261 agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacggctc
     1321 aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc
     1381 ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga
     1441 gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat
     1501 acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag
     1561 acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta
     1621 gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg
     1681 ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg
     1741 cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc
     1801 aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc
     1861 gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg
     1921 gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg
     1981 ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca
     2041 ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc
     2101 gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag
     2161 agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca
     2221 aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt
     2281 tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta
     2341 ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg
     2401 agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc
     2461 gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg
     2521 gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg
     2581 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     2641 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     2701 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     2761 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     2821 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     2881 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     2941 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     3001 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     3061 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     3121 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     3181 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     3241 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     3301 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     3361 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     3421 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     3481 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     3541 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     3601 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     3661 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata
     3721 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     3781 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     3841 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     3901 gtgcataact gtctggccag cgcacagccc aagagctgca aaaagcgcct acccttcggt
     3961 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     4021 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     4081 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     4141 aacctctgac acatgcagct cccggaaacg gtcacagctt gtctgtaagc ggatgccggg
     4201 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     4261 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     4321 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     4381 accgcatcag gccctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4981 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     5041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     5101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     5161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtgctga gagatcccct
     5221 cataatttcc ccaaagcgta accatgtgtg aataaatttt gagctagtag ggttgcagcc
     5281 acgagtaagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag
     5341 cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa
     5401 ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt
     5461 agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg
     5521 taggcaggac ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata
     5581 ttaacaagtt gtttgggtgt tcgaatttca acaggtaagt tagttgctag aatccatggc
     5641 tcctttgccg acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag
     5701 cgctgatttt ttcggccttt agagcgagat ttatacaata gaatttggca tgagattgga
     5761 ttgcttttag tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt
     5821 aagttgctga taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt
     5881 actcgactta accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag
     5941 ccagcatcac tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct
     6001 agaaattggt catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa
     6061 agagtaacag aacgaccgtg tagtgcgact gaagctcgca ataccataag ccgtttttgc
     6121 tcacggatat cagaccagtc aacaagtaca atgggcatcg tattgcccga acagataaag
     6181 ctagcatgcc aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat
     6241 cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt
     6301 tcggtaagag tgagagtttt acagtcaagt aaggcgtggc aagccaacgt taagctgttg
     6361 agtcgtttta agtgtaattc ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt
     6421 tcgcacattt tgttgtctga ttattgattt ttggcgaaac catttgatca tatgacaaga
     6481 tgtgtatcta ccttaactta atgattttga taaaaatcat taggggattc atcagcgctc
     6541 agtggaacga aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt
     6601 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     6661 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     6721 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     6781 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     6841 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     6901 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     6961 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     7021 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     7081 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     7141 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     7201 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     7261 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     7321 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     7381 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     7441 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
     7501 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
     7561 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     7621 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
     7681 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
     7741 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
     7801 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
     7861 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt
     7921 ttagtactgg attttggttt taggaattag aaattttatt gatagaagta ttttacaaat
     7981 acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc
     8041 ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat
     8101 cgacagatcc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc
     8161 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     8221 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     8281 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     8341 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc
     8401 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     8461 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     8521 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     8581 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     8641 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     8701 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     8761 tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa
     8821 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     8881 tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg
     8941 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     9001 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     9061 cctccgagag ctgcatcagg tcggacacgc tgtcgaactt ttcgatcaga aacttctcga
     9121 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggat ctgcgaaagc
     9181 tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa
     9241 atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc
     9301 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     9361 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     9421 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     9481 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
     9541 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
     9601 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttcacatca atccacttgc
     9661 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     9721 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcaatggaat ccgaggaggt ttcccgatat
     9901 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
     9961 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
    10021 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    10081 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    10141 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    10201 caatttcaca caggaaacag ctatgacatg attacgaatt cgagctcggt acccggggat
    10261 cggcgcgccg gagctgcagt gcagcgtgac ccggtcgtgc ccctctcttg agataaggtg
    10321 agcattgcat gtctaagtta taaaaaatta ccacatattt tttttgtcac acttgtttga
    10381 agtgcagttt atctatcttt atacatatat ttaaacttta ctctacgaat aatataatct
    10441 atagtactac aataatatca gtgttttaga gaatcatata aatgaacagt tagacatggt
    10501 ctaaaggaca attgagtatt ttgacaacag gactctacag ttttatcttt ttagtgtgca
    10561 tgtgttctcc tttttttttg caaatagctt cacctatata atacttcatc cattttatta
    10621 gtacatccat ttagggttta gggttaatgg tttttataga ctaatttttt tagtacatct
    10681 attttattct attttagcct ctaaattaag aaaactaaaa ctctatttta gtttttttat
    10741 ttaataattt agatataaaa tagaataaaa taaagtgact aaaaattaaa caaataccct
    10801 ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt cgagtagata atgccagcct
    10861 gttaaacgcc gtcgacgagt ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg
    10921 gccaagcgaa gcagacggca cggcatctct gtcgctgcct ctggacccct ctcgagagtt
    10981 ccgctccacc gttggacttg ctccgctgtc ggcatccaga aattgcgtgg cggagcggca
    11041 gacgtgagcc ggcacggcag gcggcctcct cctcctctca cggcaccggc agctacgggg
    11101 gattcctttc ccaccgctcc ttcgctttcc cttcctcgcc cgccgtaata aatagacacc
    11161 ccctccacac cctctttccc caacctcgtg ttgttcggag cgcacacaca cacaaccaga
    11221 actcccccaa atccacccgt cggcacctcc gcttcaaggt acgccgctcg tcctcccccc
    11281 cccccctctc taccttctca agatcggcgt tccggtccat ggttagggcc cggtagttct
    11341 acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgcta ctagcgttcg
    11401 tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct
    11461 ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt
    11521 ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac
    11581 ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg
    11641 ttgggcggtc gttcaagatc ggagtagaat taattctgtt tcaaactacc tggtggattt
    11701 attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat tgaagatgat
    11761 ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat
    11821 acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc ggtcgttcat
    11881 tcgttcaaga tcggagtaga atactgtttc aaactacctg gtgtatttat taattttgga
    11941 actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg gaaatatcga
    12001 tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg
    12061 cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt
    12121 ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga
    12181 tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg
    12241 ctcaccctgt tgtttggtgt tacttctgca gcctgcagga atggctccta agaagaagcg
    12301 gaaggttggt attcacgggg tgcctgcggc ttctgaacca ccgagagcag agacattcgt
    12361 gttcctcgat ctggaggcca cgggattgcc caacatggac ccggagatcg ccgaaattag
    12421 cctattcgcc gtccatcgtt cctccctcga aaatccagaa agagatgatt ctggttcgtt
    12481 ggtcctcccg agggtgttgg acaagctcac cctgtgcatg tgccccgaga ggccttttac
    12541 tgcaaaggcc tccgaaataa ctggattgtc aagtgagtcc ctcatgcact gcggcaaggc
    12601 cggcttcaac ggcgccgtgg tgagaacatt acaaggtttt ttatcaaggc aggaggggcc
    12661 catctgcctt gtagctcata atggatttga ttatgacttc ccgctgcttt gtacagagct
    12721 gcagcgcctc ggcgcacacc tcccacaaga tactgtttgt ctggacacgc tgccagctct
    12781 ccgcggcctc gaccgcgccc actcccatgg caccagggcg caggggagga agtcctactc
    12841 ccttgcttcc ctgttccacc ggtacttcca agctgaacct agcgcggcgc attcagcgga
    12901 gggcgacgtg cacacattgc ttctaatctt cctccacagg gcgccggagc tgctggcctg
    12961 ggctgatgag caggcgcggt cgtgggcaca tattgagccc atgtacgtgc cgccggacgg
    13021 gccaagtctt gaggctggtg gaggcggaag tggaggtggc gggtcagggg gtggcggatc
    13081 tggttcgatg gctccgaaga agaagaggaa ggttggcatc cacggggtgc cagctgcttg
    13141 caccaaggag tcggaaaagc tcaataagaa cgcggattac tacattggtc tggacatggg
    13201 cacatcctca gctggctggg cggtgagtga ctccgagtac aatctaatcc gcagaaaagg
    13261 caaggatttg tggggcgttc gccagttcga agaagcgaaa acggcggcgg aacgccgggg
    13321 gttccgggtc gctcgtcgaa ggaaacagag gcagcaggtc aggaatcgac tattgagtga
    13381 ggaatttcag aatgaaatca caaagatcga ttctgggttt ttaaaaagaa tggaggattc
    13441 tcggtttgtt attagcgaca agagggtgcc agagaagtac accctgttca atgattccgg
    13501 gtacacagat gtagaatact acaaccagta cccgaccatc taccatctga gaaaagcctt
    13561 aatcgaatcc aatgagaggt ttgatattcg ccttgtattc ctgggaatcc actcgttgtt
    13621 ccagcatcca ggccatttcc tcgacaaggg ggacgtggat acggacaata caggtcccga
    13681 agaactgata cagtttctcg aagactgcat gaacgagata caaatcagca tcccgctcgt
    13741 ctcaaaccaa aaagtcttaa ctgatatctt gacggatagc cgaataacga ggcgtgacaa
    13801 agaacagcaa attcttgaga tattgcaacc caacaaagag tccaaaaagg ccgtttccca
    13861 gttcgtgaag gttctcacag gtcaaaaagc caagttaggc gatctcatta tgatggaaga
    13921 taaagatacg gaggagtaca agtattcatt ttctttccgc gaaaagaccc tggaggagat
    13981 tcttcccgat atagagggcg tcatcgacgg tcttgcgctt gagtatatcg aatctatcta
    14041 ctcgctatat tcatggagcc tcctcaacag ctacatgaaa gatacattga ccggtcatta
    14101 ttactcgtat ctagctgagg cccgcgtggc ggcgtacgat aagcatcata gtgatcttgt
    14161 taagctgaaa acattgttcc gcgagtatat cccggaagaa tacgacaact tcttccgcaa
    14221 gatggagaag gccaactatt cgcattatat tggcagcacc gagtatgatg gtgagaagcg
    14281 ttgtagaaca gccaaagcca agcaagagga cttctacaag agtatcaaca aaatgctcga
    14341 aaagatccct gagtgcagtg aaaaaacgga gatccagaag gagataatag agggcacttt
    14401 cctccttaag caaactgggc ctcagaacgg ctttgtcccg aatcagttac agctaaaaga
    14461 actacgaaag atcctgcaaa atgcgtccaa gcactacccc tttctaacag agaaggatga
    14521 aagagatatg actgccattg acagaatcga ggcgctgttc agctttagaa tcccgtacta
    14581 catcggccca ctcaagaaca cggacaatca agggcacggt tgggctgtta ggagggacgg
    14641 gcacgagcaa atcccggtac ggccgtggaa cttcgaagaa atcattgatg agtccgctag
    14701 cgccgatctg ttcatcaaga atttggtgaa cagttgtact tacctcagga ccgagaaggt
    14761 actcccaaaa tctagcttgc tgtatcaaga attcgaagtc ctgaatgagc tcaacaacct
    14821 caggataaat gggatgtacc cagatgaaat tcagccaggc ctaaagcgta tgatcttcga
    14881 gcagtgcttc tattctggca aaaaagtcac tggaaaaaaa ctgcagttat tcctcaggag
    14941 tgtgctcacc aattcttcaa cggaggagtt tgttctaaca ggcattgaca aggactttaa
    15001 atcgtccctg tcgagttaca aaaaattctg cgagcttttt ggcgttaaga cactgaacga
    15061 tacacagaag gtgatggctg aacagattat tgaatggtcc actgtctatg gcgattcgcg
    15121 caagtttctt aagagaaagc tcgaggataa ctatccggaa cttacagatc aacaaattcg
    15181 acggattgct ggcttcaagt tctccgagtg ggggaacctg agcagggcat tcctcgagat
    15241 ggaggggtac aaggacgaag caggaaatcc tgttaccata attagagcgc ttcgtgatac
    15301 tcaaaaaaac ttgatgcagc tcctctccaa cgactcagca ttcgctaaga agctgcagga
    15361 gttaaatgac tacgtgacgc gcgacatctg gtcaatagag ccggacgacc tcgatggaat
    15421 gtatctgtct gctcctgttc gccggatgat atggcagaca tttttgatcc tcagagaagt
    15481 ggtcgatacc attggttatt cacctaagaa gattttcatg gagatggcgc ggggggagca
    15541 ggagaagaag aggactgcgt ctaggaagaa acagttgata gatctctaca aggaggcagg
    15601 catgaagaac gatgaattgt ttggagatct cgagagcctg gaagaggccc aattgaggtc
    15661 caagaagctc tatctttatt tccggcagat ggggagggat atttatagtg gaaagctaat
    15721 tgatttcatg gatgtactcc acggcaatcg gtacgacata gaccacatcc acccgcaaag
    15781 caagaagaaa gatgactcct tagaaaacaa tctggtactc acaagcaagg acttcaataa
    15841 tcatattaaa caagacgtct accccattcc tgagcaaatt caatcgagac aaaaaggatt
    15901 ttgggccatg ctcctgaagc aaggtttcat gtctcaggag aaatacaacc gactcatgcg
    15961 gacgactccg ttcaccgatg aggagctggc ggagttcgtc aacagacaac tggtggagac
    16021 tagacaggga acaaaagcaa ttatatcact catcaaccag tgtttccctg actcagaagt
    16081 tgtgtacgtg aaggccggaa acacctcgga cttccggcag cggttcgaca ttccaaagtc
    16141 gcgggacttg aacaattacc accacgccgt agacgcctat cttaacatcg ttgtcggcaa
    16201 cgtttatgac accaaattta ccaaaaatcc aatcaatttt attaagaaga tgaggaaatc
    16261 tggtaatttg cactcctata gtttaagaag gatgtatgat ttcaatgtcc agcgcggaga
    16321 tcagaccgcg tgggttgccg aaaatgacac gacgttaaaa acagtgaaga agacagcttt
    16381 taagacctcg cccatggtga caaaacggac gtacgagagg aaaggtgggt tggcagattc
    16441 cgtcctgata gccgccaaga aggcaaagcc tggggtccat cttccagtga aaacttcaga
    16501 ctcacgcttt gcaaaccagg tgtccaccta cggcggctac gacaacgtga agggaagcca
    16561 cttctttctt gttgagcatc agcagaagaa aaaaacaata aggagcatag agaatgtccc
    16621 gatccatctt aaagagaagt taaagaccaa ggaggaactc gaacactact gtgcacaagt
    16681 gctcggcatg gtgcagcctg atgtccgtct gactcgcatc ccaatgtact ctcttctatt
    16741 gattgatggc tattactatt acctgaccgg acgtacgggt ggcaatctgt ctctctctaa
    16801 tgcggttgag ctctgcttgc cagccaagga gcaggcacat atcaggatga tttcaaaaat
    16861 agccgggggg cgctcaacgg acgctttgtc tgcggaggcc aaggacgatt ttcgcaagaa
    16921 aaatcttcgg ctctatgacg aactcgctga aaagcaccgg tcaactattt tttcgaaacg
    16981 caagaacccc atcggaccca agctgctgaa gtacagagag gcatttgtga aacaaactat
    17041 cgagaatcaa tgcaaggtta ttttacagat attgaagttg accagcacaa actgcaaaac
    17101 ttctgctgac cttaagctta tcggaggttc agggcaagaa ggcgtcatga gtatttccaa
    17161 gctcctacga gctgaaaagt atgcagaatt ttacctaatt tgtcagtccc ctagcggaat
    17221 ttatgagact cgaaagaacc tgctgactat aaagcggcca gcggcgacga agaaggcggg
    17281 gcaggcgaag aagaagaagt gagcttctcg agtatatgaa gatgaagatg aaatatttgg
    17341 tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta
    17401 tgaatttgtg gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca
    17461 aatgtttcta taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact
    17521 gtatgtgcta tggtatggac tatggaatat gattaaagat aagcgctgag ctcggacggc
    17581 gcgccggagc acatcagtct ctgcacaaag tgcatcctgg gctgcttcaa ttataaagcc
    17641 ccattcacca catttgctag atagtcgaaa agcaccatca atattgagct tcaggtattt
    17701 ttggttgtgt tgtggttgga ttgattctaa tatataccaa atcaatataa ttcactacca
    17761 aaatatacca tagccatcac aactttatta attttggtag cttaagatgg tatatataat
    17821 aaccaattaa caactgattc taattttact acggcccagt atctaccaat acaaaacaac
    17881 gagtatgttt tcttccgtcg taatcgtaca cagtacaaaa aaacctggcc agcctttctt
    17941 gggctggggc tctctttcga aaggtcacaa aacgtacacg gcagtaacgc cgcttcgctg
    18001 cgtgttaacg gccaccaacc ccgccgtgag caaacggcat cagctttcca cctcctcgat
    18061 atctccgcgg cgccgtctgg acccgccccc tttccgttcc tttctttcct tctcgcgttt
    18121 gcgtggtggg gacggactcc ccaaaccgcc tctccctctc tttatttgtc tatattctca
    18181 ctgggcccca cccaccgcac ccctgggccc actcacgagt ccccccctcc ccacctataa
    18241 ataccccacc ccctcctcgc ctcttcctcc atcaatcgaa tccccaaaat cgcagagaaa
    18301 aaaaaatctc ccctcgaagc gaagcgtcga atcgccttct caaggtatgc gattttctga
    18361 tcctctccgt tcctcgcgtt tgatttgatt tcccggcctg ttcgtgattg tgagatgttg
    18421 ttgttagtct ccgttttgcg atctgtggta gatttgaaca gggttagatg gggttcgcgt
    18481 ggtatgctgg atctgtgatt atgagcgatg ctgttcgtgg tccaagtatt tattggttcg
    18541 gatctagaag tagaactgtg ctagggttgt gatttgttcc gatctgttca atcagtagga
    18601 tttagtctct gtttttctcg ttgatccaag tagcagcttc aggtatattt tgcttaggtt
    18661 gtttttgatt cagtccctct agttgcatag attctactct gttcatgttt aatctaaggg
    18721 ctgcgtcttg ttgattagtg attacatagc atagctttca ggatatttta cttgcttatg
    18781 cctatcttat caactgttgc acctgtaaat tctagcctat gttaattaac ctgccttatg
    18841 tgctctcggg atagtgctag tagttattga atcagtttgc cgatggaatt ctagtagttc
    18901 atagacctgc agattatttt tgtgaactcg agcacggtgc gactctctat tttgttaggt
    18961 cactgttggt gttgataggt acactgatgt tattgtggtt tagatcgtgt atctaacata
    19021 ttggaataat ttgattgact gatttctgct gtacttgctt ggtattgtta taatttcatg
    19081 ttcatagttg ctgaccatgc ttcggtaatt gtgtgtgcag cctgcaggaa caaagcacca
    19141 gtggtctagt ggtagaatag taccctgcca cggtacagac ccgggttcga ttcccggctg
    19201 gtgcaggaga ccttacagtt ggacacaggc caacttgtga agattgcaaa acgtggcggc
    19261 gtgaccgcaa tggaggcagt gcatgcatcg cgcaatgcac tgacgggtgc ccccctggag
    19321 acgggcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
    19381 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    19441 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    19501 tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt
    19561 cctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
    19621 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    19681 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    19741 gctcactgcc cgctttccac cggtggtctc agtttgagtg tcttgttaag aaattaacaa
    19801 gatgagttca aatcaggctc ctagagagat ccgaacttac cttcatggcg ggcattgtgc
    19861 ccaacaaagc accagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt
    19921 tcgattcccg gctggtgcag gtactcgagc ctagacttgt ccatcttctg gattggccaa
    19981 gttaattaat gtatgaaata aaaggatgca cacatagtga catgctaatc actataatgt
    20041 gggcatcaaa gttgtgtgtt atgtgtaatt actaattatc tgaataagag aaagagatca
    20101 tccatatttc ttatcctaaa tgaatgtcac gtgtctttat aattctttga tgaaccagat
    20161 gcattttatt aaccaattcc atatacatat aaatattaat catatataat taatatcaat
    20221 tgggttagca aaacaaatct agtctaggtg tgttttgccg ctgagctccc ggggcgcgcc
    20281 gatatcgagc tcagtgtggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
    20341 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
    20401 gaagaggccc gcaccgatcg cccttc
//
