LOCUS       sequence-430365-       15606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HIV reporter vector expressing dGFP and mThy1.2 from Env locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Peterson JJ, Lewis CA, Burgos SD, Manickam A, Xu Y, Rowley AA, 
            Clutton G, Richardson B, Zou F, Simon JM, Margolis DM, Goonetilleke 
            N, Browne EP
  TITLE     A histone deacetylase network regulates epigenetic reprogramming and
            viral silencing in HIV-infected cells.
  JOURNAL   Cell Chem Biol. 2023 Dec 21;30(12):1617-1633.e9. doi: 
            10.1016/j.chembiol.2023.11.009.
  PUBMED    38134881
REFERENCE   2  (bases 1 to 15606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2023.11"; journalName: "Cell Chem Biol"; date: 
            "2023-12-21- 21"; volume: "30"; issue: "12"; pages: "1617-1633.e9"
COMMENT     Sequence Label: NL4-3-Delta-6-drEGFP-IRES-mThy1.2
FEATURES             Location/Qualifiers
     source          1..15606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             790..2292
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARA**LSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQI*HQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2085..5096
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIWPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4781..4898
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             join(6313..6315,6316..6318,6319..7029,7030..7032,7033..7035,
                     7036..7158)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to a mutated version 
                     of residues 422-461 of mouse ornithine decarboxylase, 
                     giving an in vivo half-life of ~1 hour"
                     /label=d1EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKEFSHGFPPAVAAQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(6358..6379)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6619..6638)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6966..6987
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7166..7739
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7189..7741
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7333..7350)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7560..7579
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7750..8238
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.2
                     /note="This allele of Thy1 is also known as CD90.2."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELQVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    8748..8981
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9166..9210
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9504..9530
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     LTR             9857..10490
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12130..12147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12301..12889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12381..12400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13060..13851,13852..13920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13683..13702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13921..14025)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14093..14111
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(14149..14171)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     14271..14290
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtaataa ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatctaaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
     1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
     1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc
     2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
     2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggag
     2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc
     2281 tcgtcacaat aaagataggg gggcaattaa aggaagctct attagataca ggagcagatg
     2341 atacagtatt agaagaaatg aatttgccag gaagatggaa accaaaaatg atagggggaa
     2401 ttggaggttt tatcaaagta agacagtatg atcagatact catagaaatc tgcggacata
     2461 aagctatagg tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt
     2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa
     2581 aattaaagcc aggaatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa
     2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattt
     2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat
     2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc
     2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg
     2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta
     2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac
     3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt
     3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat
     3121 ctgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga
     3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg
     3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca
     3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt
     3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag
     3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa
     3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga
     3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa
     3601 caggaaagta tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg
     3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat
     3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga
     3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga
     3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta
     3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg
     3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat
     4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag
     4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag
     4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat
     4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag
     4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg
     4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc
     4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa
     4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag
     4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa
     4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt
     4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa
     4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
     4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
     4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt
     5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca
     5101 tggaaaagat tagtaaaaca ccatatgtag atttcaagga aagctaagga ctggttttat
     5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg
     5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat
     5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct
     5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata
     5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac
     5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag
     5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagact
     5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga
     5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg
     5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc
     5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
     5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
     5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
     5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
     6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgta
     6061 atgcaacctt aatagtagca atagtagcat tagtagtagc aataataata gcaatagttg
     6121 tgtggtccat agtaatcata gaatatagga aaatattaag acaaagaaaa atagacaggt
     6181 taattgatag actaatagaa agagcagaag acagtggcaa tgagagtgaa ggagaagtat
     6241 cagcacttgt ggagatgggg gtggaaatgg ggcaccatgc gccgtgggac atcgatgatc
     6301 tgtagttcta gaatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     6361 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     6421 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     6481 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     6541 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     6601 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     6661 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     6721 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     6781 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     6841 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     6901 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     6961 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     7021 ctgtacaagg aattcagcca tggcttcccg ccggcggtgg cggcgcagga tgatggcacg
     7081 ctgcccatgt cttgtgccca ggagagcggg atggaccgtc accctgcagc ctgtgcttct
     7141 gctaggatca atgtgtaggc tagcgcccct ctccctcccc cccccctaac gttactggcc
     7201 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     7261 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     7321 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     7381 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     7441 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     7501 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     7561 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     7621 tgggatctga tctggggcct cggtacacat gctttacatg tgtttagtcg aggttaaaaa
     7681 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     7741 atggccacaa tgaacccagc catcagcgtc gctctcctgc tctcagtctt gcaggtgtcc
     7801 cgagggcaga aggtgaccag cctgacagcc tgcctggtga accaaaacct tcgcctggac
     7861 tgccgccatg agaataacac caaggataac tccatccagc atgagttcag cctgacccga
     7921 gagaagagga agcacgtgct ctcaggcacc cttgggatac ccgagcacac gtaccgctcc
     7981 cgcgtcaccc tctccaacca gccctatatc aaggtcctta ccctagccaa cttcaccacc
     8041 aaggatgagg gcgactactt ttgtgagctt caagtctcgg gcgcgaatcc catgagctcc
     8101 aataaaagta tcagtgtgta tagagacaag ctggtcaagt gtggcggcat aagcctgctg
     8161 gttcagaaca catcctggat gctgctgctg ctgctttccc tctccctcct ccaagccctg
     8221 gacttcattt ctctgtgagc tagcaaatta agagaacaat ttggaaataa taaaacaata
     8281 atctttaagc aatcctcagg aggggaccca gaaattgtaa cgcacagttt taattgtgga
     8341 ggggaatttt tctactgtaa ttcaacacaa ctgtttaata gtacttggtt taatagtact
     8401 tggagtactg aagggtcaaa taacactgaa ggaagtgaca caatcacact cccatgcaga
     8461 ataaaacaat ttataaacat gtggcaggaa gtaggaaaag caatgtatgc ccctcccatc
     8521 agtggacaaa ttagatgttc atcaaatatt actgggctgc tattaacaag agatggtggt
     8581 aataacaaca atgggtccga gatcttcaga cctggaggag gcgatatgag ggacaattgg
     8641 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     8701 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     8761 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     8821 caggccagac aattattgtc tgatatagtg cagcagcaga acaatttgct gagggctatt
     8881 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaaacagct ccaggcaaga
     8941 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     9001 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     9061 gaacagattt ggaataacat gacctggatg gagtgggaca gagaaattaa caattacaca
     9121 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     9181 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     9241 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     9301 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     9361 cacctcccaa tcccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     9421 agagacagag acagatccat tcgattagtg aacggatcct tagcacttat ctgggacgat
     9481 ctgcggagcc tgtgcctctt cagctaccac cgcttgagag acttactctt gattgtaacg
     9541 aggattgtgg aacttctggg acgcaggggg tgggaagccc tcaaatattg gtggaatctc
     9601 ctacagtatt ggagtcagga actaaagaat agtgctgttc cagcagcaga tggggtggga
     9661 gcagtatctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctaac
     9721 aatgctgctt gtgcctggct agaagcacaa gaggaggaag aggtgggttt tccagtcaca
     9781 cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     9841 aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     9901 ctgtggatct accacacaca aggctacttc cctgattggc agaactacac accagggcca
     9961 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
    10021 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
    10081 ggaatggatg accctgagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
    10141 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgaca tcgagcttgc
    10201 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
    10261 tggcgagccc tcagatgctg catataagca gctgcttttt gcctgtactg ggtctctctg
    10321 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10381 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10441 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca cccaggaggt
    10501 agaggttgca gtgagccaag atcgcgccac tgcattccag cctgggcaag aaaacaagac
    10561 tgtctaaaat aataataata agttaagggt attaaatata tttatacatg gaggtcataa
    10621 aaatatatat atttgggctg ggcgcagtgg ctcacacctg cgcccggccc tttgggaggc
    10681 cgaggcaggt ggatcacctg agtttgggag ttccagacca gcctgaccaa catggagaaa
    10741 ccccttctct gtgtattttt agtagatttt attttatgtg tattttattc acaggtattt
    10801 ctggaaaact gaaactgttt ttcctctact ctgataccac aagaatcatc agcacagagg
    10861 aagacttctg tgatcaaatg tggtgggaga gggaggtttt caccagcaca tgagcagtca
    10921 gttctgccgc agactcggcg ggtgtccttc ggttcagttc caacaccgcc tgcctggaga
    10981 gaggtcagac cacagggtga gggctcagtc cccaagacat aaacacccaa gacataaaca
    11041 cccaacaggt ccaccccgcc tgctgcccag gcagagccga ttcaccaaga cgggaattag
    11101 gatagagaaa gagtaagtca cacagagccg gctgtgcggg agaacggagt tctattatga
    11161 ctcaaatcag tctccccaag cattcgggga tcagagtttt taaggataac ttagtgtgta
    11221 gggggccagt gagttggaga tgaaagcgta gggagtcgaa ggtgtccttt tgcgccgagt
    11281 cagttcctgg gtgggggcca caagatcgga tgagccagtt tatcaatccg ggggtgccag
    11341 ctgatccatg gagtgcaggg tctgcaaaat atctcaagca ctgattgatc ttaggtttta
    11401 caatagtgat gttaccccag gaacaatttg gggaaggtca gaatcttgta gcctgtagct
    11461 gcatgactcc taaaccataa tttctttttt gttttttttt ttttattttt gagacagggt
    11521 ctcactctgt cacctaggct ggagtgcagt ggtgcaatca cagctcactg cagcctcaac
    11581 gtcgtaagct caagcgatcc tcccacctca gcctgcctgg tagctgagac tacaagcgac
    11641 gccccagtta atttttgtat ttttggtaga ggcagcgttt tgccgtgtgg ccctggctgg
    11701 tctcgaactc ctgggctcaa gtgatccagc ctcagcctcc caaagtgctg ggacaaccgg
    11761 ggccagtcac tgcacctggc cctaaaccat aatttctaat cttttggcta atttgttagt
    11821 cctacaaagg cagtctagtc cccaggcaaa aagggggttt gtttcgggaa agggctgtta
    11881 ctgtctttgt ttcaaactat aaactaagtt cctcctaaac ttagttcggc ctacacccag
    11941 gaatgaacaa ggagagcttg gaggttagaa gcacgatgga attggttagg tcagatctct
    12001 ttcactgtct gagttataat tttgcaatgg tggttcaaag actgcccgct tctgacacca
    12061 gtcgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    12121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    12181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    12241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    12301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    12361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    12421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    12481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    12541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    12601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    12661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    12721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    12781 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    12841 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    12901 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    12961 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    13021 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    13081 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    13141 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    13201 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    13261 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    13321 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    13381 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    13441 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    13501 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    13561 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    13621 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    13681 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    13741 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    13801 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    13861 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    13921 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    13981 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    14041 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    14101 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    14161 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    14221 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
    14281 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    14341 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    14401 ggtgcgggcc tcttcgctat tacgccaggg gaggcagaga ttgcagtaag ctgagatcgc
    14461 agcactgcac tccagcctgg gcgacagagt aagactctgt ctcaaaaata aaataaataa
    14521 atcaatcaga tattccaatc ttttccttta tttatttatt tattttctat tttggaaaca
    14581 cagtccttcc ttattccaga attacacata tattctattt ttctttatat gctccagttt
    14641 tttttagacc ttcacctgaa atgtgtgtat acaaaatcta ggccagtcca gcagagccta
    14701 aaggtaaaaa ataaaataat aaaaaataaa taaaatctag ctcactcctt cacatcaaaa
    14761 tggagataca gctgttagca ttaaatacca aataacccat cttgtcctca ataattttaa
    14821 gcgcctctct ccaccacatc taactcctgt caaaggcatg tgccccttcc gggcgctctg
    14881 ctgtgctgcc aaccaactgg catgtggact ctgcagggtc cctaactgcc aagccccaca
    14941 gtgtgccctg aggctgcccc ttccttctag cggctgcccc cactcggctt tgctttccct
    15001 agtttcagtt acttgcgttc agccaaggtc tgaaactagg tgcgcacaga gcggtaagac
    15061 tgcgagagaa agagaccagc tttacagggg gtttatcaca gtgcaccctg acagtcgtca
    15121 gcctcacagg gggtttatca cattgcaccc tgacagtcgt cagcctcaca gggggtttat
    15181 cacagtgcac ccttacaatc attccatttg attcacaatt tttttagtct ctactgtgcc
    15241 taacttgtaa gttaaatttg atcagaggtg tgttcccaga ggggaaaaca gtatatacag
    15301 ggttcagtac tatcgcattt caggcctcca cctgggtctt ggaatgtgtc ccccgagggg
    15361 tgatgactac ctcagttgga tctccacagg tcacagtgac acaagataac caagacacct
    15421 cccaaggcta ccacaatggg ccgccctcca cgtgcacatg gccggaggaa ctgccatgtc
    15481 ggaggtgcaa gcacacctgc gcatcagagt ccttggtgtg gagggaggga ccagcgcagc
    15541 ttccagccat ccacctgatg aacagaacct agggaaagcc ccagttctac ttacaccagg
    15601 aaaggc
//
