LOCUS       sequence-430381-        9851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 
            (Saccharomyces cerevisiae). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   He CW, Cui XF, Ma SJ, Xu Q, Ran YP, Chen WZ, Mu JX, Li H, Zhu J, 
            Gong Q, Xie Z
  TITLE     Membrane recruitment of Atg8 by Hfl1 facilitates turnover of 
            vacuolar membrane proteins in yeast cells approaching stationary 
            phase.
  JOURNAL   BMC Biol. 2021 Jun 4;19(1):117. doi: 10.1186/s12915-021-01048-7.
  PUBMED    34088313
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2021-06-4"; volume: "19(1):117. doi"; pages: " 
            10.1186/s12915-021-01048-7"
COMMENT     Sequence Label: BS-ZJLEU-PTEF1-HFL1-W371A I375A D384A Y387A-GFP
FEATURES             Location/Qualifiers
     source          1..9851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1508..2218
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(1535..1563)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2158..2178
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2250..2415
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2305..2329)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3693..3712)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3694..3712)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3719..3736)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3719..3735)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3728..3750)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3876..4331
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3963..3982)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4173..4194
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4687..4706)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4806..4828
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4866..4884)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4969..5073
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5074..5142,5143..5934)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5292..5311)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6105..6693
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6594..6613
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6847..6864
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6981..7002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7017..7022,7023..7040,7041..7047)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7055..7071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7060..7082
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7079..7095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7079..7095
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7116..7134
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7422..7829
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        join(9649..9851,1..211)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt
       61 ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa cggtcttcaa
      121 tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgctca
      181 ttagaaagaa agcatagcaa tctaatctaa gttttaatta caaatgctcg agatggaaaa
      241 caaactactt tgttggtggt tgtactggcc atgcgtgtat tcatctatta tagctaccat
      301 aatatctttt tacaccataa ctagacactt attaaattac cgtaaaccat atgaacaaag
      361 actatccata cggatactat tgctcgtgcc catttttagt gtgtcatgcg caagtggcat
      421 aattaagcct gaagcagctc aattttatgt cgatcctatc agagaatttt atgaagcatt
      481 cgttatttac acttttttca catttttaac actactacta ggaggcgaga ggaacatcat
      541 tactgtttta agcttaaatc acgcccccac aagacatccg ataccgctaa taggtaaaat
      601 ttgcaaaccg atagacttat ctgatccttt tgattttctt tttgtcaaaa aaggcatatt
      661 acagtatgtt tggttcaaac cattttattg ttttggtaca ttgatatgca gtgcatggaa
      721 gctacccaaa ttcgaaatat ttttgaacgt tttttataat atttcagtga cttggtcact
      781 gtatagttta gcactatttt ggaagtgtct ctatccggaa ttaactcctt ataaaccatg
      841 gttgaaattt ttatgtgtca aattgattat ttttgcctct tattggcaga gcatcataat
      901 ccagggtcta gtggttactg gaaagttggg tacgggaaac caagatcgaa cctctggata
      961 tgtgtacaag aatgggctcc tgtgtataga gatggtgcct ttcgccatct tgcatgcagt
     1021 agctttccct tggaacaaat acacagcatt tagcatacct tatggggcca ggatgaaatt
     1081 catttacgct ttgaaagatt ttcttggttg cggtgatcta atatgggatt ttaaacagac
     1141 tttgtttgct ggtcccttgt actataatta taggaacttc gatcctgaag ccatggatct
     1201 attaagcact agacaacaat ccggtgcgac tatggaaaga ctaaagcatg ggctaagatt
     1261 tactgataat ggaagaaata gttattgggt ggcatatggg agtatagata acaaccttgt
     1321 tccggaatca atagaggaat cagcggaaga tgatgctgct ggtcagagaa cttttcctga
     1381 ggctccaaat gctccggttg ttcacgacta cacgatgggc catcgatata gtagatcaat
     1441 gaatgattta agaagagatg ttcaaagtag gtcatcaatg gcttgtcgga tccccgggtt
     1501 aattaacagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1561 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     1621 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     1681 acttgtcact actttcactt atggtgttca atgcttttca agatacccag atcatatgaa
     1741 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     1801 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     1861 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     1921 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     1981 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     2041 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     2101 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     2161 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     2221 gtaaggatcc tgagtaaata agctgccgcg cgaatttctt atgatttatg atttttatta
     2281 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
     2341 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
     2401 agcatgaggt cgctcttatt gaccacaccg agctctaagc aaatagctaa attatatacg
     2461 aattaatatt atgattaagt gtttacgtga gtgcgatatt tttattacta tcttatacag
     2521 ttgtatatac tctataaaat gagttgtcta ttaattaacg cgatgaatgc tttctgggtt
     2581 tacctctcca acaactctag tttacttctc aatacattca attgtatttg atttgtcaat
     2641 acttcatcat taatcaattc tatagttttg tttttctcgt ttatttccaa atttaatgca
     2701 tcaattttat tattcaattt gtcgttgatt ttggttaatg attttatggt ttgatctctg
     2761 gcattgattg tttgtgttag tttttcatta ttgataatta aattatttaa gttagttatc
     2821 aactcggtgt tttcaagttt caagttttca atttctttag agtttattag atttgtcaaa
     2881 gtttctgaat tgcttgattg gtcctgtaga agagtatttg ttgttgtgga taattgattc
     2941 aatttttgag acaattgctg gaaggcgttg aaatatctag catcaatctc atggtttttt
     3001 tcccgagagt ctcgtagatt caattgtttt aatatatctt gggaccactc ttgatttgaa
     3061 ctcatggaaa ttaaactggg tgttgtgttg tggtgtaatg attgtacccc ctttgcttat
     3121 aattgtgtgg ccaccgaagt cggtgatgct gtcgccgaag aagttaagaa aatccttgct
     3181 taaaaagatt ctcttttttt atgatatttg tacataaact ttataaatga aattcataat
     3241 agaaacgaca cgaaattaca aaatggaata tgttcatagg gtagacgaaa ctatatacgc
     3301 aatctacata catttatcaa gaaggagaaa aaggaggatg taaaggaata caggtaagca
     3361 aattgatact aatggctcaa cgtgataagg aaaaagaatt gcactttaac attaatattg
     3421 acaaggagga gggcaccaca caaaaagtta ggtgtaacag aaaatcatga aactatgatt
     3481 cctaatttat atattggagg attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca
     3541 ctcaatgacc tgaccatttg atggagttta agtcaatacc tccttgaacc atttcccata
     3601 atggtgaaag ttccctcaag aattttactc tgtcagaaac ggccttaacg acgtaggccg
     3661 gccatttaaa tgtttaaacg gccacgctgg ccccctatag tgagtcgtat tacaattcac
     3721 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     3781 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     3841 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
     3901 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3961 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4021 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4081 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4141 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4201 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4261 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4321 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4381 atagggtaat aactgatata attaaattga agctctaatt tgtgagttta gtattaggat
     4441 ctctaccctt ggcgaaaagt cctctgccaa caatgatgat atctgatcca ccacttacaa
     4501 cttcgtcgac ggttctgtac tgctgaccca atatgcgtat atataccaat ctaagtctgt
     4561 gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa
     4621 accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaaggtgg tatggtgcac
     4681 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     4741 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4801 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     4861 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     4921 gacgtcggcg cgccgaggac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4981 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5041 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5101 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5161 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5221 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5281 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5341 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5401 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5461 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5521 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5581 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5641 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5701 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5761 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5821 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5881 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5941 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6001 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6061 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6121 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6181 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6241 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6301 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6361 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6421 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6481 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6541 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6601 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6661 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6721 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6781 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6841 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6901 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6961 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7021 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7081 ggaaacagct atgaccatga ttacgccaag ctcggaatta accctcacta aagggaacaa
     7141 aagctgggcc ggccatttaa atgtttaaac ggccacgctg gccgaggaga acttctagta
     7201 tatctacata cctaatatta ttgccttatt aaaaatggaa tcccaacgat tacatcaaaa
     7261 tccacattct cttcaaaatc aattgtcctg tacttccttg ttcatgtgtg ttcaaaaacg
     7321 ttatatttat aggataatta tactctattt ctcaacaagt aattggttgt ttggccgagc
     7381 ggtctaaggc gcctgattca agaaatatct tgaccgcagt taactgtggg aatactcagg
     7441 tatcgtaaga tgcaagagtt cgaatctctt agcaaccatt atttttttcc tcaacataac
     7501 gagaacacac aggggcgcta tcgcacagaa tcaaattcga tgactggaaa ttttttgtta
     7561 atttcagagg tcgcctgacg catatacctt tttcaactga aaaattggga gaaaaaggaa
     7621 aggtgagagc gccggaaccg gcttttcata tagaatagag aagcgttcat gactaaatgc
     7681 ttgcatcaca atacttgaag ttgacaatat tatttaagga cctattgttt tttccaatag
     7741 gtggttagca atcgtcttac tttctaactt ttcttacctt ttacatttca gcaatatata
     7801 tatatatatt tcaaggatat accattctaa tgtctgcccc taagttacca atagatagcg
     7861 gtttaatata aggtttcaac aagaaatatt ttgtgaaaat caaaagttaa tagtttcact
     7921 gtcgaaaata tgaaagttta agttttcagt tcatcccatt gtagttatca aatttctgtg
     7981 tttcccgtat atgtgtgctc tgtattcatc gctatcttga aactggttat gatcgttgcg
     8041 catgtgacct gtatcgctca aaagagcccg gcaccggtac cgggcaaaag cccctagcaa
     8101 gctaaaatga ttcaactttc gtagtaattt gttcaaccgg tcacagtaaa gtcaatgact
     8161 aacttttgaa tcgaggatgt atttccaatt gaaagcaatg tatatataaa ccattgtgtt
     8221 gaagcttgtc ttacagtttt cttgtatcct cccattgttc atatagtact taaagtacca
     8281 taatccttaa agaacaaact tgaaagagaa tacactatac acaactaata aataaagaat
     8341 aatcatggct gtgaccaaga caattgtagt tctaccaggt gaccatgttg gtcaagaaat
     8401 cactgaagag gccattaaag ttttaaatgc tattcaggaa tgtcgtccag acaaggtcaa
     8461 tttcaagttt gatcatcatt tgatcggtgg tgctgcaatt gatgccactg gtgttccatt
     8521 accagacgaa gctttggagg cctctaagaa agctgatgcc gtgcttctgg gtgctgttgg
     8581 tggtccaaaa tggggtactg gcgctgtcag accagaacaa ggtcttttga aaatccgtaa
     8641 ggagttgcaa ttgtatgcta atctaagacc atgtaatttt gcatctgatt ccttactaga
     8701 tctatcgcca ttgaagcctg aaattgcaag aggtacagat ttcgtcgttg ttagagaact
     8761 agtgggtggt atttatttcg gggagagaaa agaagatgaa ggtgatggtg tcgcctggga
     8821 tagcgaaaag tattctgtgc ccgaagttca aagaatcact agaatggctg ctttcatggc
     8881 tctgcaacac aacccacctt tacctatttg gtctctagat aaagccaacg ttctagcatc
     8941 atcaagatta tggagaaaaa cagtggaaga gacgattaag aatgaattcc cacaattgac
     9001 tgttcaacat caattgattg attccgctgc tatgatcttg gttaagaacc caactcatct
     9061 aaatggtatt attatcacaa acaatatgtt tggtgatatt atctccgatg aagcctccgt
     9121 gatcccaggt tcgttaggat tacttccatc tgcctccttg gcatctcttc cagataagaa
     9181 tacggcattt ggtttatatg agccatgtca tggctctgct ccagacttac caaagggaaa
     9241 ggttaacccc gtagcaacaa tcctatctgc tgctatgatg ttgaagttgt ctctggacct
     9301 gttcgaagaa ggtgaaatta tagaacaagc agtcaagaaa gtgttggatt ctggtatcag
     9361 aacagcggat ctaaagggta ccaactctac tactgaggtc ggtgatgcag tagcgaaagc
     9421 tgtcagggaa ctattagctt agacttagta gatgtacagt tgcatacata agtctgaaca
     9481 gaaagaagta aatatcatat ctagttcggt ttagaaatca gtgactatat tggttttgac
     9541 cagatcgcaa tccttgcaag catttagtaa tgactatcaa accggtgaat catctgataa
     9601 ggctatatta gcttgtgcat tcgcatgtat cgggaaacga acggtaccgg gcccccacac
     9661 accatagctt caaaatgttt ctactccttt tttactcttc cagattttct cggactccgc
     9721 gcatcgccgt accacttcaa aacacccaag cacagcatac taaatttccc ctctttcttc
     9781 ctctagggtg tcgttaatta cccgtactaa aggtttggaa aagaaaaaag agaccgcctc
     9841 gtttcttttt c
//
