LOCUS       sequence-430385-        9851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of Hfl1 point mutant. Uses auxotrophic marker LEU2 
            (Saccharomyces cerevisiae). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   He CW, Cui XF, Ma SJ, Xu Q, Ran YP, Chen WZ, Mu JX, Li H, Zhu J, 
            Gong Q, Xie Z
  TITLE     Membrane recruitment of Atg8 by Hfl1 facilitates turnover of 
            vacuolar membrane proteins in yeast cells approaching stationary 
            phase.
  JOURNAL   BMC Biol. 2021 Jun 4;19(1):117. doi: 10.1186/s12915-021-01048-7.
  PUBMED    34088313
REFERENCE   2  (bases 1 to 9851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2021-06-4"; volume: "19(1):117. doi"; pages: " 
            10.1186/s12915-021-01048-7"
COMMENT     Sequence Label: BS-ZJLEU-PTEF1-HFL1-D384A-GFP
FEATURES             Location/Qualifiers
     source          1..9851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(2035..2442)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        complement(2730..2748)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2769..2785)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2769..2785)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2782..2804)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2793..2809
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2817..2823,2824..2841,2842..2847))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2862..2883
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3000..3017)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3171..3759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3251..3270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3930..4721,4722..4790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4553..4572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4791..4895)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4980..4998
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5036..5058)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5158..5177
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5533..5988)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5670..5691)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5882..5901
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6114..6136
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6128..6145
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6129..6145
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6152..6171
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6152..6170
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(7449..7614)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     7535..7559
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(7646..8356)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(7686..7706)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     8301..8329
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     promoter        complement(join(9653..9851,1..215))
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 tttttcgacg aagaaaaaga aacgaggcgg tctctttttt cttttccaaa cctttagtac
       61 gggtaattaa cgacacccta gaggaagaaa gaggggaaat ttagtatgct gtgcttgggt
      121 gttttgaagt ggtacggcga tgcgcggagt ccgagaaaat ctggaagagt aaaaaaggag
      181 tagaaacatt ttgaagctat ggtgtgtggg ggcccggtac cgttcgtttc ccgatacatg
      241 cgaatgcaca agctaatata gccttatcag atgattcacc ggtttgatag tcattactaa
      301 atgcttgcaa ggattgcgat ctggtcaaaa ccaatatagt cactgatttc taaaccgaac
      361 tagatatgat atttacttct ttctgttcag acttatgtat gcaactgtac atctactaag
      421 tctaagctaa tagttccctg acagctttcg ctactgcatc accgacctca gtagtagagt
      481 tggtaccctt tagatccgct gttctgatac cagaatccaa cactttcttg actgcttgtt
      541 ctataatttc accttcttcg aacaggtcca gagacaactt caacatcata gcagcagata
      601 ggattgttgc tacggggtta acctttccct ttggtaagtc tggagcagag ccatgacatg
      661 gctcatataa accaaatgcc gtattcttat ctggaagaga tgccaaggag gcagatggaa
      721 gtaatcctaa cgaacctggg atcacggagg cttcatcgga gataatatca ccaaacatat
      781 tgtttgtgat aataatacca tttagatgag ttgggttctt aaccaagatc atagcagcgg
      841 aatcaatcaa ttgatgttga acagtcaatt gtgggaattc attcttaatc gtctcttcca
      901 ctgtttttct ccataatctt gatgatgcta gaacgttggc tttatctaga gaccaaatag
      961 gtaaaggtgg gttgtgttgc agagccatga aagcagccat tctagtgatt ctttgaactt
     1021 cgggcacaga atacttttcg ctatcccagg cgacaccatc accttcatct tcttttctct
     1081 ccccgaaata aataccaccc actagttctc taacaacgac gaaatctgta cctcttgcaa
     1141 tttcaggctt caatggcgat agatctagta aggaatcaga tgcaaaatta catggtctta
     1201 gattagcata caattgcaac tccttacgga ttttcaaaag accttgttct ggtctgacag
     1261 cgccagtacc ccattttgga ccaccaacag cacccagaag cacggcatca gctttcttag
     1321 aggcctccaa agcttcgtct ggtaatggaa caccagtggc atcaattgca gcaccaccga
     1381 tcaaatgatg atcaaacttg aaattgacct tgtctggacg acattcctga atagcattta
     1441 aaactttaat ggcctcttca gtgatttctt gaccaacatg gtcacctggt agaactacaa
     1501 ttgtcttggt cacagccatg attattcttt atttattagt tgtgtatagt gtattctctt
     1561 tcaagtttgt tctttaagga ttatggtact ttaagtacta tatgaacaat gggaggatac
     1621 aagaaaactg taagacaagc ttcaacacaa tggtttatat atacattgct ttcaattgga
     1681 aatacatcct cgattcaaaa gttagtcatt gactttactg tgaccggttg aacaaattac
     1741 tacgaaagtt gaatcatttt agcttgctag gggcttttgc ccggtaccgg tgccgggctc
     1801 ttttgagcga tacaggtcac atgcgcaacg atcataacca gtttcaagat agcgatgaat
     1861 acagagcaca catatacggg aaacacagaa atttgataac tacaatggga tgaactgaaa
     1921 acttaaactt tcatattttc gacagtgaaa ctattaactt ttgattttca caaaatattt
     1981 cttgttgaaa ccttatatta aaccgctatc tattggtaac ttaggggcag acattagaat
     2041 ggtatatcct tgaaatatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag
     2101 aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgt
     2161 caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt ctatatgaaa
     2221 agccggttcc ggcgctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat
     2281 atgcgtcagg cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg
     2341 cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat
     2401 tcgaactctt gcatcttacg atacctgagt attcccacag ttaactgcgg tcaagatatt
     2461 tcttgaatca ggcgccttag accgctcggc caaacaacca attacttgtt gagaaataga
     2521 gtataattat cctataaata taacgttttt gaacacacat gaacaaggaa gtacaggaca
     2581 attgattttg aagagaatgt ggattttgat gtaatcgttg ggattccatt tttaataagg
     2641 caataatatt aggtatgtag atatactaga agttctcctc ggccagcgtg gccgtttaaa
     2701 catttaaatg gccggcccag cttttgttcc ctttagtgag ggttaattcc gagcttggcg
     2761 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2821 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2881 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2941 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3601 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4921 gacgtcctcg gcgcgccgac gtctaagaaa ccattattat catgacatta acctataaaa
     4981 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     5041 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     5101 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     5161 catcagagca gattgtactg agagtgcacc ataccacctt ttcaattcat catttttttt
     5221 ttattctttt ttttgatttc ggtttccttg aaattttttt gattcggtaa tctccgaaca
     5281 gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca tattgggtca
     5341 gcagtacaga accgtcgacg aagttgtaag tggtggatca gatatcatca ttgttggcag
     5401 aggacttttc gccaagggta gagatcctaa tactaaactc acaaattaga gcttcaattt
     5461 aattatatca gttattaccc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     5521 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     5581 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     5641 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     5701 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     5761 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     5821 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     5881 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     5941 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
     6001 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     6061 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     6121 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gggccagcgt
     6181 ggccgtttaa acatttaaat ggccggccta cgtcgttaag gccgtttctg acagagtaaa
     6241 attcttgagg gaactttcac cattatggga aatggttcaa ggaggtattg acttaaactc
     6301 catcaaatgg tcaggtcatt gagtgttttt tatttgttgt attttttttt ttttagagaa
     6361 aatcctccaa tatataaatt aggaatcata gtttcatgat tttctgttac acctaacttt
     6421 ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctt tttccttatc
     6481 acgttgagcc attagtatca atttgcttac ctgtattcct ttacatcctc ctttttctcc
     6541 ttcttgataa atgtatgtag attgcgtata tagtttcgtc taccctatga acatattcca
     6601 ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat gtacaaatat
     6661 cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc gacagcatca
     6721 ccgacttcgg tggccacaca attataagca aagggggtac aatcattaca ccacaacaca
     6781 acacccagtt taatttccat gagttcaaat caagagtggt cccaagatat attaaaacaa
     6841 ttgaatctac gagactctcg ggaaaaaaac catgagattg atgctagata tttcaacgcc
     6901 ttccagcaat tgtctcaaaa attgaatcaa ttatccacaa caacaaatac tcttctacag
     6961 gaccaatcaa gcaattcaga aactttgaca aatctaataa actctaaaga aattgaaaac
     7021 ttgaaacttg aaaacaccga gttgataact aacttaaata atttaattat caataatgaa
     7081 aaactaacac aaacaatcaa tgccagagat caaaccataa aatcattaac caaaatcaac
     7141 gacaaattga ataataaaat tgatgcatta aatttggaaa taaacgagaa aaacaaaact
     7201 atagaattga ttaatgatga agtattgaca aatcaaatac aattgaatgt attgagaagt
     7261 aaactagagt tgttggagag gtaaacccag aaagcattca tcgcgttaat taatagacaa
     7321 ctcattttat agagtatata caactgtata agatagtaat aaaaatatcg cactcacgta
     7381 aacacttaat cataatatta attcgtatat aatttagcta tttgcttaga gctcggtgtg
     7441 gtcaataaga gcgacctcat gctatacctg agaaagcaac ctgacctaca ggaaagagtt
     7501 actcaagaat aagaattttc gttttaaaac ctaagagtca ctttaaaatt tgtatacact
     7561 tatttttttt ataacttatt taataataaa aatcataaat cataagaaat tcgcgcggca
     7621 gcttatttac tcaggatcct tactatttgt atagttcatc catgccatgt gtaatcccag
     7681 cagctgttac aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa
     7741 gggcagattg tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg
     7801 gagtattttg ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa
     7861 ttttgaagtt aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg
     7921 agttatagtt gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac
     7981 cttttaactc gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct
     8041 tgtagttccc gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg
     8101 cactcttgaa aaagtcatgc cgtttcatat gatctgggta tcttgaaaag cattgaacac
     8161 cataagtgaa agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa
     8221 taaatttaag ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa
     8281 atttgtgccc attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa
     8341 gttcttctcc tttactgtta attaacccgg ggatccgaca agccattgat gacctacttt
     8401 gaacatctct tcttaaatca ttcattgatc tactatatcg atggcccatc gtgtagtcgt
     8461 gaacaaccgg ataatttggc gcctcaggaa aagttctctg accagcaata tcatcttccc
     8521 atgattcctc tattgattcc ggaacaaggt tgttatctat actcccatat gccacccaat
     8581 aactatttct tccattatca gtaaatctta gcccatgctt tagtctttcc atagtcgcac
     8641 cggattgttg tctagtgctt aatagatcca tggcttcagg atcgaagttc ctataattat
     8701 agtacaaggg accagcaaac aaagtctgtt taaaatccca tattagatca ccgcaaccaa
     8761 gaaaatcttt caaagcgtaa atgaatttca tcctggcccc ataaggtatg ctaaatgctg
     8821 tgtatttgtt ccaagggaaa gctactgcat gcaagatggc gaaaggcacc atctctatac
     8881 acaggagccc attcttgtac acatatccag aggttcgatc ttggtttccc gtacccaact
     8941 ttccagtaac cactagaccc tggattatga tgctctgcca ataagaggca aaaataatca
     9001 atttgacaca taaaaatttc aaccatggtt tataaggagt taattccgga tagagacact
     9061 tccaaaatag tgctaaacta tacagtgacc aagtcactga aatattataa aaaacgttca
     9121 aaaatatttc gaatttgggt agcttccatg cactgcatat caatgtacca aaacaataaa
     9181 atggtttgaa ccaaacatac tgtaatatgc cttttttgac aaaaagaaaa tcaaaaggat
     9241 cagataagtc tatcggtttg caaattttac ctattagcgg tatcggatgt cttgtggggg
     9301 cgtgatttaa gcttaaaaca gtaatgatgt tcctctcgcc tcctagtagt agtgttaaaa
     9361 atgtgaaaaa agtgtaaata acgaatgctt cataaaattc tctgatagga tcgacataaa
     9421 attgagctgc ttcaggctta attatgccac ttgcgcatga cacactaaaa atgggcacga
     9481 gcaatagtat ccgtatggat agtctttgtt catatggttt acggtaattt aataagtgtc
     9541 tagttatggt gtaaaaagat attatggtag ctataataga tgaatacacg catggccagt
     9601 acaaccacca acaaagtagt ttgttttcca tctcgagcat ttgtaattaa aacttagatt
     9661 agattgctat gctttctttc taatgagcaa gaagtaaaaa aagttgtaat agaacaagaa
     9721 aaatgaaact gaaacttgag aaattgaaga ccgtttatta acttaaatat caatgggagg
     9781 tcatcgaaag agaaaaaaat caaaaaaaaa aattttcaag aaaaagaaac gtgataaaaa
     9841 tttttattgc c
//
