LOCUS       sequence-430389-        9360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of hsOPA1 isoform 5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Song Z, Chen H, Fiket M, Alexander C, Chan DC
  TITLE     OPA1 processing controls mitochondrial fusion and is regulated by 
            mRNA splicing, membrane potential, and Yme1L.
  JOURNAL   J Cell Biol. 2007 Aug 27;178(5):749-55. Epub 2007 Aug 20.
  PUBMED    17709429
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2007-08-27"; volume: "178(5)"; pages: "749-55. Epub 
            2007 Aug 20"
COMMENT     Sequence Label: OPA1-isoform5
FEATURES             Location/Qualifiers
     source          1..9360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     polyA_signal    4399..4520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4410..4429)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4464..4483
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        4534..5033
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4569..4592)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4966..4985
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5054..5653
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5054..5073)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5550..5570
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5753..6267
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6429..6445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6453..6459,6460..6477,6478..6483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6498..6519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6636..6653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6807..7395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6887..6906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7566..8357,8358..8426))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8189..8208
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8427..8531)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8599..8617
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8655..8677)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8777..8796
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8990..9012
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9140..9159
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9359..9360,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga gaattcacgc gtggtaccat
     1441 gtggcgacta cgtcgggccg ctgtggcctg tgaggtctgc cagtctttag tgaaacacag
     1501 ctctggaata aaaggaagtt taccactaca aaaactacat ctggtttcac gaagcattta
     1561 tcattcacat catcctacct taaagcttca acgaccccaa ttaaggacat cctttcagca
     1621 gttctcttct ctgacaaacc ttcctttacg taaactgaaa ttctctccaa ttaaatatgg
     1681 ctaccagcct cgcaggaatt tttggccagc aagattagct acgagactct taaaacttcg
     1741 ctatctcata ctaggatcgg ctgttggggg tggctacaca gccaaaaaga cttttgatca
     1801 gtggaaagat atgataccgg accttagtga atataaatgg attgtgcctg acattgtgtg
     1861 ggaaattgat gagtatatcg attttgagaa aattagaaaa gcccttccta attcagaaga
     1921 ccttgtaaag ttagcaccag actttgacaa gattgttgaa agccttagct tattgaagga
     1981 cttttttacc tcaggtcaca aattggttag tgaagtcata ggagcttctg acctacttct
     2041 cttgttaggt tctccggaag aaacggcgtt tagagcaaca gatcgtggat ctgaaagtga
     2101 caagcatttt agaaaggtgt cagacaaaga gaaaattgac caacttcagg aagaacttct
     2161 gcacactcag ttgaagtatc agagaatctt ggaacgatta gaaaaggaga acaaagaatt
     2221 gagaaaatta gtattgcaga aagatgacaa aggcattcat catagaaagc ttaagaaatc
     2281 tttgattgac atgtattctg aagttcttga tgttctctct gattatgatg ccagttataa
     2341 tacgcaagat catctgccac gggttgttgt ggttggagat cagagtgctg gaaagactag
     2401 tgtgttggaa atgattgccc aagctcgaat attcccaaga ggatctgggg agatgatgac
     2461 acgttctcca gttaaggtga ctctgagtga aggtcctcac catgtggccc tatttaaaga
     2521 tagttctcgg gagtttgatc ttaccaaaga agaagatctt gcagcattaa gacatgaaat
     2581 agaacttcga atgaggaaaa atgtgaaaga aggctgtacc gttagccctg agaccatatc
     2641 cttaaatgta aaaggccctg gactacagag gatggtgctt gttgacttac caggtgtgat
     2701 taatactgtg acatcaggca tggctcctga cacaaaggaa actattttca gtatcagcaa
     2761 agcttacatg cagaatccta atgccatcat actgtgtatt caagatggat ctgtggatgc
     2821 tgaacgcagt attgttacag acttggtcag tcaaatggac cctcatggaa ggagaaccat
     2881 attcgttttg accaaagtag acctggcaga gaaaaatgta gccagtccaa gcaggattca
     2941 gcagataatt gaaggaaagc tcttcccaat gaaagcttta ggttattttg ctgttgtaac
     3001 aggaaaaggg aacagctctg aaagcattga agctataaga gaatatgaag aagagttttt
     3061 tcagaattca aagctcctaa agacaagcat gctaaaggca caccaagtga ctacaagaaa
     3121 tttaagcctt gcagtatcag actgcttttg gaaaatggta cgagagtctg ttgaacaaca
     3181 ggctgatagt ttcaaagcaa cacgttttaa ccttgaaact gaatggaaga ataactatcc
     3241 tcgcctgcgg gaacttgacc ggaatgaact atttgaaaaa gctaaaaatg aaatccttga
     3301 tgaagttatc agtctgagcc aggttacacc aaaacattgg gaggaaatcc ttcaacaatc
     3361 tttgtgggaa agagtatcaa ctcatgtgat tgaaaacatc taccttccag ctgcgcagac
     3421 catgaattca ggaactttta acaccacagt ggatatcaag cttaaacagt ggactgataa
     3481 acaacttcct aataaagcag tagaggttgc ttgggagacc ctacaagaag aattttcccg
     3541 ctttatgaca gaaccgaaag ggaaagagca tgatgacata tttgataaac ttaaagaggc
     3601 cgttaaggaa gaaagtatta aacgacacaa gtggaatgac tttgcggagg acagcttgag
     3661 ggttattcaa cacaatgctt tggaagaccg atccatatct gataaacagc aatgggatgc
     3721 agctatttat tttatggaag aggctctgca ggctcgtctc aaggatactg aaaatgcaat
     3781 tgaaaacatg gtgggtccag actggaaaaa gaggtggtta tactggaaga atcggaccca
     3841 agaacagtgt gttcacaatg aaaccaagaa tgaattggag aagatgttga aatgtaatga
     3901 ggagcaccca gcttatcttg caagtgatga aataaccaca gtccggaaga accttgaatc
     3961 ccgaggagta gaagtagatc caagcttgat taaggatact tggcatcaag tttatagaag
     4021 acatttttta aaaacagctc taaaccattg taacctttgt cgaagaggtt tttattacta
     4081 ccaaaggcat tttgtagatt ctgagttgga atgcaatgat gtggtcttgt tttggcgtat
     4141 acagcgcatg cttgctatca ccgcaaatac tttaaggcaa caacttacaa atactgaagt
     4201 taggcgatta gagaaaaatg ttaaagaggt attggaagat tttgctgaag atggtgagaa
     4261 gaagattaaa ttgcttactg gtaaacgcgt tcaactggcg gaagacctca agaaagttag
     4321 agaaattcaa gaaaaacttg atgctttcat tgaagctctt catcaggaga aataagcggc
     4381 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     4441 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     4501 taagctgcaa taaacaagtt aacgaattct accgggtagg ggaggcgctt ttcccaaggc
     4561 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     4621 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     4681 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     4741 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     4801 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     4861 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     4921 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     4981 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     5041 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5101 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     5161 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     5221 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     5281 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     5341 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     5401 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     5461 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     5521 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     5581 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     5641 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     5701 ccatgcatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     5761 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaat
     5821 acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca gcagaatatg
     5881 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     5941 gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     6001 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     6061 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     6121 ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca ataaaccctc
     6181 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     6241 gactacccgt cagcgggggt ctttcatggg taacagtttc ttgaagttgg agaacaacat
     6301 tctgagggta ggagtcgaat attaagtaat cctgactcaa ttagccactg ttttgaatcc
     6361 acatactcca atactcctga aatagttcat tatggacagc gcagaaagag ctggggagaa
     6421 ttgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     6481 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     6541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     6601 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     6661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     6781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     7501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     7561 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     7621 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     7681 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     7741 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     7801 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     7861 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7921 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7981 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8041 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8101 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8161 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8221 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8281 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8341 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8401 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     8461 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     8521 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     8581 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     8641 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     8701 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     8761 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     8821 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     8881 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     8941 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9001 cgttgtaaaa cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     9061 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     9121 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     9181 cgccggtgat gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag
     9241 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     9301 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
//
