LOCUS       sequence-430390-        6063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500
            / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 6063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..6063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..870
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    2791..2912
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2828..2847)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2882..2901
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2919..3374)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3056..3077)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3268..3287
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3401..3505
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3507..3864
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3507..3698
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3530..3550)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3715..3850
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3899..4693
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3899..4693
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3953..3972)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4563..4582
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4881..4900)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4925..4972
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5301..5889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5790..5809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggagaa aagtttcgtg ataactgatc cacggctgcc agacaatccc
      601 atcatcttcg catccgatgg cttcctggag ctgaccgagt attccagaga ggagatcctg
      661 ggccgcaatg gccgctttct gcagggacca gagacagacc aggccacagt gcagaagatt
      721 cgcgatgcca ttagagatca gcgcgagatt accgtgcagc tgataaacta cacaaaaagc
      781 gggaagaaat tctggaacct cctgcacctc cagcccatga gggaccagaa gggtgagctc
      841 cagtatttca tcggagtgca gctggatgga tccggactca gatctcgagc tcaagcttcg
      901 atggcgactg cgacccccgt gccgccgcgg atgggcagcc gcgctggcgg ccccaccacg
      961 ccgctgagcc ccacgcgcct gtcgcggctc caggagaagg aggagctgcg cgagctcaat
     1021 gaccggctgg cggtgtacat cgacaaggtg cgcagcctgg agacggagaa cagcgcgctg
     1081 cagctgcagg tgacggagcg cgaggaggtg cgcggccgtg agctcaccgg cctcaaggcg
     1141 ctctacgaga ccgagctggc cgacgcgcga cgcgcgctcg acgacacggc ccgcgagcgc
     1201 gccaagctgc agatcgagct gggcaagtgc aaggcggaac acgaccagct gctcctcaac
     1261 tatgctaaga aggaatctga tcttaatggc gcccagatca agcttcgaga atatgaagca
     1321 gcactcaatt cgaaagatgc agctcttgct actgcacttg gtgacaaaaa aagtttagag
     1381 ggagatttgg aggatctgaa ggatcagatt gcccagttgg aagcctcctt agctgcagcc
     1441 aaaaaacagt tagcagatga aactttactt aaagtagatt tggagaatcg ttgtcagagc
     1501 cttactgagg acttggagtt tcgcaaaagc atgtatgaag aggagattaa cgagaccaga
     1561 aggaagcatg aaacgcgctt ggtagaggtg gattctgggc gtcaaattga gtatgagtac
     1621 aagctggcgc aagcccttca tgagatgaga gagcaacatg atgcccaagt gaggctgtat
     1681 aaggaggagc tggagcagac ttaccatgcc aaacttgaga atgccagact gtcatcagag
     1741 atgaatactt ctactgtcaa cagtgccagg gaagaactga tggaaagccg catgagaatt
     1801 gagagccttt catcccagct ttctaatcta cagaaagagt ctagagcatg tttggaaagg
     1861 attcaagaat tagaggactt gcttgctaaa gaaaaagaca actctcgtcg catgctgaca
     1921 gacaaagaga gagagatggc ggaaataagg gatcaaatgc agcaacagct gaatgactat
     1981 gaacagcttc ttgatgtaaa gttagccctg gacatggaaa tcagtgctta caggaaactc
     2041 ttagaaggcg aagaagagag gttgaagctg tctccaagcc cttcttcccg tgtgacagta
     2101 tcccgagcat cctcaagtcg tagtgtacgt acaactagag gaaagcggaa gagggttgat
     2161 gtggaagaat cagaggcgag tagtagtgtt agcatctctc attccgcctc agccactgga
     2221 aatgtttgca tcgaagaaat tgatgttgat gggaaattta tccgcttgaa gaacacttct
     2281 gaacaggatc aaccaatggg aggctgggag atgatcagaa aaattggaga cacatcagtc
     2341 agttataaat atacctcaag atatgtgctg aaggcaggcc agactgttac aatttgggct
     2401 gcaaacgctg gtgtcacagc cagcccccca actgacctca tctggaagaa ccagaactcg
     2461 tggggcactg gcgaagatgt gaaggttata ttgaaaaatt ctcagggaga ggaggttgct
     2521 caaagaagta cagtctttaa aacaaccata cctgaagaag aggaggagga ggaagaagca
     2581 gctggagtgg ttgttgagga agaacttttc caccagcagg gaaccccaag agcatccaat
     2641 agaagctgtg caattatgta gggatccacc ggatctagat aactgatcat aatcagccat
     2701 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     2761 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     2821 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2881 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     2941 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     3001 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     3061 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     3121 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     3181 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     3241 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     3301 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     3361 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3421 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3481 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     3541 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3601 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3661 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3721 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3781 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3841 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     3901 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3961 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     4021 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     4081 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4141 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4201 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4261 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4321 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4381 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     4441 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4501 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4561 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4621 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4681 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     4741 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4801 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4861 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     4921 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     4981 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     5041 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     5101 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     5161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5941 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     6001 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     6061 ccg
//
