LOCUS       sequence-430408-        8235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in One IQ transgene switch, empty vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Hong J, Sohn KC, Park HW, Jeon H, Ju E, Lee JG, Lee JS, Rho J, Hur 
            GM, Ro H
  TITLE     All-in-one IQ toggle switches with high versatilities for 
            fine-tuning of transgene expression in mammalian cells and tissues.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Feb 2;32(1):101202. doi: 
            10.1016/j.omtm.2024.101202. eCollection 2024 Mar 14.
  PUBMED    38374964
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-02-2"; volume: "32(1):101202. doi"; 
            pages: " 10.1016/j.omtm.2024.101202. eCollection 2024 Mar 14"
COMMENT     Sequence Label: pSIQ
FEATURES             Location/Qualifiers
     source          1..8235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(563..583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        568..897
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      748..883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             990..1589
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(990..1009)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1486..1506
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1819..1953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1972..2071
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        2240..2619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2620..2823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2774..2794
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2868..2886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4689..4707)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4727..4744)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4733..4957
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5290..5359
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5683..5703
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6048..6066
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    6187..6321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6360..6459)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6477..6495)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6500..6516)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6500..6516)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6618..6637)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6629..7438
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6696..6715)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      7585..8173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8074..8093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaact gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      601 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      661 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      721 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      781 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      841 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      901 cttgcatgcc tgcaggtcgg ccgccacgac cggtgccgcc accatcccct gacccacgcc
      961 cctgacccct cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg
     1021 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     1081 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     1141 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     1201 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     1261 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     1321 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     1381 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     1441 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     1501 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     1561 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     1621 aaaggagcgc acgaccccat ggctccgacc gaagccaccc ggggcggccc cgccgacccc
     1681 gcacccgccc ccgaggccca ccgactctag aggatcataa tcagccatac cacatttgta
     1741 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     1801 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     1921 aaactcatca atgtatctta tcatgtctgg atcctcgtta agctcgggcc ccaaataatg
     1981 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     2041 ataatgccaa ctttgtacaa aaaagcaggc tccgataccg ctcgccgcag ccgaacgacc
     2101 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     2161 acgcatctgt gcggtatttc acaccgcata gttaggcgtt ttgcgctgct tcgcgatgta
     2221 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     2281 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2341 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2401 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2461 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2521 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2581 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2641 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2701 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2761 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2821 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     2881 tatagggaga cccaagctgc caacatgccg cctaaacgca agacactcaa tgccgctgcc
     2941 gaagccaatg cccacgctga tggccatgct gatggcaatg ctgatggtca tgtcgctaac
     3001 actgcagcaa gcagcaacaa cgcccgtttt gcggacttga ccaacattga cacacccggc
     3061 ctcggcccta ccacgacgac gttacttgtc gagcccgctc gttcgaaacg ccagagagtc
     3121 tcgagggcct gtgatcagtg tcgagctgca cgtgaaaagt gtgatggaat ccagccggct
     3181 tgcttcccct gtgtgtcgca gggccggtcg tgtacctacc aggccagtcc caagaagcga
     3241 ggagtccaga cgggctacat ccgcactctc gaactggctc tggcttggat gttcgagaac
     3301 gttgcccgca gcgaggacgc cctccacaat cttttggtcc gtgatgctgg ccagggcagc
     3361 gctctcctgg tcggcaaaga ctcgcctgct gcagaacgcc tgcatgcaag atgggcgacg
     3421 agtcgagtca acaaaagcat cacccgtctt ctctcaggtc aggccgcaca agatccatct
     3481 gaagacggcc aatccccgtc cgaagacata aatgtccaag atcaggccgc acaagatcca
     3541 tctgaagacg gccaatcccc gtccgaagac ataaatgtcc aagatgagtt cctggatatg
     3601 gccgacttcg agtttgagca gatgtttacc gatgcccttg gaattgacga gtacggtggg
     3661 ctgcagatga ggcctgaatg tgtcatacag gagcccagta aaaataaaga caggcaaaga
     3721 caaaagaaag acaaaggaat attattacct gttagtacga ccacagtcga agaccacatg
     3781 cccccgatca tgcaatgtga tccacctccg cccgaggccg ccaggattca cgaagtcgtc
     3841 ccgaggtatc tttcggagaa gctgatggag cagaacaggc agaagaacat accaccattg
     3901 tcggcgaatc agaagtctct gatcgcgagg ctcgtgtggt accaggaggg atatgagcag
     3961 ccctccgacg aggatctcaa aagagtaacg cagacttggc agtcggatga agaggacgag
     4021 gaatccgatc tacccttccg ccagatcacg gagatgacga tcttaacggt ccagttgatc
     4081 gtcgagttcg ccaagggtct accgggcttt tcgaagatat cacagtctga tcaaatcacc
     4141 ttattaaaag cctcgtccag cgaggtgatg atgctgcggg tggcgaggcg atacgacgcc
     4201 gcgtccgaca gcattctgtt cgccaacaac aaggcgtaca cgcgcgacaa ctaccgcaag
     4261 gcgggcatgg ccgaggtcat cgaagacctc ctacacttct gccggtgcat gttcgcgatg
     4321 ggcatggaca atgtgcactt tgcactgctc acggccatcg ttatattctc agatcggccc
     4381 gggctcgagc agccgtcgct ggtagaagag atccagagat actacctgaa cacgttgcga
     4441 atttacatca tcaaccagaa cagcgcgtcg tcgcgctgcg ccgtgatcta cggcaggatc
     4501 ctgagcgtgc tgaccgagct acgcacgctc ggcacgcaaa actccaacat gtgcatctcg
     4561 ctgaagctga agaacaggaa gctgccgccg ttcctcgagg agatctggga cgtggcggag
     4621 gtggccacga cgcatcccac ggtgctgccg cccaccaacc cggtggtgct atagctagag
     4681 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     4741 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4801 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4861 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4921 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagttgagag
     4981 gacattccaa tcataggctg cccatccacc ctctgtgtcc tcctgttaat taggtcactt
     5041 aacaaaaagg aaattgggta ggggtttttc acagaccgct ttctaagggt aattttaaaa
     5101 tatctgggaa gtcccttcca ctgctgtgtt ccagaagtgt tggtaaacag cccacaaatg
     5161 tcaacagcag aaacatacaa gctgtcagct ttgcacaagg gcccaacacc ctgctcatca
     5221 agaagcactg tggttgctgt gttagtaatg tgcaaaacag gaggcacatt ttccccacct
     5281 gtgtaggttc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
     5341 gtactccacc tcacccatct atccaaaaca gccttgtggt cagtgttcat ctgctgactg
     5401 tcaactgtag cattttttgg ggttacagtt tgagcaggat atttggtcct gtagtttgct
     5461 aacacaccct gcagctccaa aggttcccca ccaacagcaa aaaaatgaaa atttgaccct
     5521 tgaatgggtt ttccagcacc attttcatga gttttttgtg tccctgaatg caagtttaac
     5581 atagcagtta ccccaataac ctcagtttta acagtaacag cttcccacat caaaatattt
     5641 ccacaggtta agtcctcatt taaattaggc aaaggaattg ctcaactttg tatagaaaag
     5701 ttggggtaat cgcttatcct cggataaaca attatcctca cgggtaatcg cttatccgct
     5761 cgggtaatcg cttatcctcg ggtaatcgct tatcctcggg taatcgctta tcctcggata
     5821 aacaattatc ctcacgggta atcgcttatc cgctcgggta atcgcttatc ctcggataaa
     5881 caattatcct cacgggtaat cgcttatccg ctcgggtaat cgcttatcct cgggtaatcg
     5941 cttatccttg caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct
     6001 cactttgagc tcctccacac gaattccctc gacctcgaag aagcttgatt taggtgacac
     6061 tatagaatac aagctacttg ttctttttgc aggatcccat cgattccgga cttaaggcgg
     6121 ccgcagtact cgattcgaat tcaaggcctc tcgagcctct agaactatag tgagtcgtat
     6181 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6241 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6301 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcagggccga
     6361 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
     6421 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
     6481 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     6541 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
     6601 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     6661 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     6721 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     6781 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
     6841 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
     6901 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
     6961 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
     7021 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
     7081 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
     7141 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
     7201 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
     7261 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
     7321 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
     7381 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
     7441 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
     7501 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
     7561 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7621 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7681 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     7741 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7801 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7861 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7921 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7981 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     8041 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     8101 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     8161 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     8221 ttttgctcac atgtt
//
