LOCUS       sequence-430419-       12963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNBAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12963)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 12963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-MS2-HA-HsNBAS_AA
FEATURES             Location/Qualifiers
     source          1..12963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      661..670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1099..1125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      1142..1151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8282..8300)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8283..8300)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8320..8337)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8326..8550
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8596..9024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8683..8702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8893..8914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9033..9053)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9038..9367
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9218..9353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9280..9299
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9434..10228
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9488..9507)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10098..10117
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10402..10523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10439..10458)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10493..10512
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10572..10588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10572..10588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10585..10607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10596..10612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10620..10626,10627..10644,10645..10650))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10665..10686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10803..10820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10974..11562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11054..11073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11733..12524,12525..12593))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12356..12375
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12594..12698)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12773..12792)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 gccaccatgg cttctaactt tactcagttc gttctcgtcg acaatggcgg aactggcgac
      721 gtgactgtcg ccccaagcaa cttcgctaac ggggtcgctg aatggatcag ctctaactcg
      781 cgatcacagg cttacaaagt aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa
      841 tacaccatca aagtcgaggt gcctaaagtg gcaacccaga ctgttggtgg tgaagagctt
      901 cctgtagccg gatggagatc ttacttaaat atggaactaa ccattccaat tttcgccacg
      961 aattccgact gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg
     1021 attccctcgg ccatcgcggc caactccggc atctacggag gtggaggtgg atctggtgga
     1081 tctcctccac atatggctta cccatacgat gttccagatt acgctcctcc aaagcttgga
     1141 tccatggcgg cccccgagtc agggccggct ttgagtccag gcactgcaga gggtgaggag
     1201 gagacgattc tctatgactt gttggtcaac accgagtggc caccggagac tgaagtacag
     1261 cctagaggca accaaaaaca tggtgcatcc tttatcatca cgaaagcaat tcgagatcgt
     1321 ttattatttt tacgccaata catctggtac agcccggcac cttttttgct ccctgatgga
     1381 ctggttcgct tggttaataa acagataaac tggcatttgg tacttgcaag caatggaaag
     1441 cttttggctg ctgttcaaga tcagtgtgtg gaaatcaggt ctgcaaaaga tgattttaca
     1501 tccattattg ggaaatgtca agttccgaaa gacccgaaac cccagtggag acgggtagca
     1561 tggagttacg attgtaccct actggcctat gccgaaagca caggaactgt gagggtgttt
     1621 gatctcatgg gaagtgaact ctttgtcatt tccccggcat ctagttttat aggtgactta
     1681 agctatgcca ttgctgggtt gatattttta gaatataaag caagtgcaca gtggtctgca
     1741 gaactcctgg tcatcaatta ccgaggagaa cttagaagtt accttgtaag tgttggaaca
     1801 aatcagagct accaagaaag tcactgtttc agcttcagta gtcattatcc tcatggaatc
     1861 aacacagcta tttaccaccc tggtcacaga cttttacttg ttggtggatg tgaaactgct
     1921 gaagtaggca tgtcaaaagc ttctagctgt ggcctttctg cctggagagt tctttcagga
     1981 tcaccgtatt ataagcaggt tactaatggt ggagacgggg ttactgcagt accgaagaca
     2041 ctgggattat taaggatgtt aagtgtcaag ttttacagtc gccagggaca agaacaggat
     2101 ggaattttta agatgagcct ttctccagat gggatgctcc tggcagccat tcacttctca
     2161 gggaaactga gcatctgggc gattccatct ctgaagcaac aaggggaatg gggtcaaaat
     2221 gagcagccag gctatgatga ccttaatcct gattggaggc tctctactga gaagagaaaa
     2281 aaaatcaaag ataaagagtc cttttaccca ctgatagatg tcaattggtg ggcagacagt
     2341 gcagtgactt tagctcgatg ctctggtgct ttaactgttt catctgtgaa aactttgaag
     2401 aatttactgg gaaaatcctg tgaatggttt gaaccatcac ctcaagtcac tgctacccat
     2461 gatgggggat ttttaagttt ggagtgtgag attaaacttg cccccaaacg atctcgtttg
     2521 gagactagag ctggagaaga agatgaagga gaagaggatt ctgattctga ttatgaaata
     2581 tctgccaagg ctcgctactt tggttatata aaacagggcc tttacttggt gactgaaatg
     2641 gagcgatttg caccaccacg gaaacgccca cgaaccatta ctaaaaacta ccgccttgtg
     2701 agtttgcgct ccacgacacc agaggaactt tatcagagga agattgaaag tgaagagtat
     2761 gaggaagcct tgtccttggc tcatacctac ggcctggata ctgaccttgt atatcagagg
     2821 cagtggagga agtcagcggt caacgttgct tcaattcaga attatttgag taaaataaag
     2881 aagcgatcct gggttctcca tgagtgtttg gaaagagttc ctgaaaatgt ggatgctgca
     2941 aaagaactgc ttcagtatgg attaaaaggc acagacctgg aggctctttt agcaataggg
     3001 aaaggagcag atgatggcag atttacatta cctggtgaaa tagacattga cagtatctcc
     3061 tatgaagagc tttcaccacc tgatgaagag cctgccaaga ataaaaagga aaaggagctc
     3121 aagaagagac aagaactact gaaattagtg aacttttcca agttgacact ggaacaaaag
     3181 gaactttgcc gttgtagacg gaagttatta acctacttag atcgacttgc aacatatgag
     3241 gaaatcctag gagtgcctca tgcatctgaa cagagatatg atgctgaatt ctttaagaaa
     3301 ttcagaaatc agaatattgt tctctcagca agaacttatg ctcaggaaag taatgtacaa
     3361 gccctggaaa ttctgtttac ttaccatggt tccgacctgc ttcctcatcg ccttgcaatt
     3421 ctgtccaact ttccagagac cacttctcca catgaatatt ctgttttgct gcccgaagct
     3481 tgttttaacg gtgactccct gatgatcatt ccttggcatg aacataaaca ccgagctaaa
     3541 gattggtgcg aggagttggc ttgcagaatg gttgttgagc cgaatctcca agatgaaagt
     3601 gaattcttgt atgctgcaca gcctgagtta ctaaggttca ggatgaccca gcttacggtg
     3661 gagaaggtta tggactggta tcagaccaga gcagaggaaa tagagcatta tgctcggcag
     3721 gtggactgtg cattgtcact tattcgactt gggatggagc ggaatattcc tggtttgctg
     3781 gttctctgtg acaatttggt tactctggaa acattggttt atgaagccag gtgtgatgta
     3841 actctaaccc tgaaagaact ccagcagatg aaagacattg aaaaactaag attactgatg
     3901 aatagttgtt ctgaggataa atatgtgaca agtgcctacc agtggatggt tccctttctt
     3961 catcgttgtg agaaacagtc gcctggtgtg gctaatgagc tattaaaaga atatttagta
     4021 actttagcta aaggggactt aaaatttccc ctgaagatat ttcagcattc caaaccagat
     4081 ctgcagcaaa aaattattcc tgatcaggac caactgatgg caatagcact agagtgcatc
     4141 tatacctgtg aacgaaatga tcaactctgt ctttgctatg acctactaga atgtctgcca
     4201 gaaagaggat atggtgataa gacagaggca accacaaagc ttcatgacat ggtagaccaa
     4261 ctggaacaaa ttctcagtgt gtcagagctt ttggaaaaac atggactcga gaaaccaatt
     4321 tcatttgtta aaaacactca atctagctca gaagaggcac gcaagctgat ggttagattg
     4381 acgaggcaca ctggccggaa gcagcctcct gtcagtgagt ctcattggag aacgttgctg
     4441 caagacatgt taactatgca gcagaatgta tacacatgtc tagattctga tgcctgctat
     4501 gagatattta cagaaagcct tctgtgctct agtcgccttg aaaacatcca cctggctgga
     4561 cagatgatgc actgcagtgc ttgttcagaa aatcctccag ctggtatagc ccataaaggg
     4621 aaaccccact acagggtcag ctacgaaaag agtattgact tggttttggc tgccagcaga
     4681 gagtacttca attcttctac caacctcact gatagctgca tggatctagc caggtgctgc
     4741 ttacaactga taacagacag accccctgcc attcaagagg agctagatct tatccaagcc
     4801 gttggatgtc ttgaagaatt tggggtaaag atcctgcctt tgcaagtgcg attgtgccct
     4861 gatcggatca gtctcatcaa ggagtgtatt tcccagtccc ccacatgcta taaacaatcc
     4921 accaagcttc tgggccttgc tgagctgctg agggttgcag gtgagaaccc agaagaaagg
     4981 cggggacagg ttctaatcct tttagtggag caggcacttc gcttccatga ctacaaagca
     5041 gccagtatgc attgtcagga gctgatggcc acaggttatc ctaaaagttg ggatgtttgt
     5101 agccagttag gacaatcaga aggttaccag gacttggcca ctcgtcaaga gctcatggct
     5161 tttgctttga cacattgccc tcctagcagc attgaacttc ttttggcagc tagcagctct
     5221 ctgcagacag aaattcttta tcaaagagtg aatttccaga tccatcatga aggaggggaa
     5281 aatatcagtg cttcaccatt aactagtaaa gcagtacaag aggatgaagt aggtgttcca
     5341 ggtagcaatt cagctgacct attgcgctgg accactgcta ccaccatgaa agtcctttcc
     5401 aacaccacaa ccaccaccaa agcggtgctg caggccgtca gtgatgggca gtggtggaag
     5461 aagtctttaa cttaccttcg accccttcag gggcaaaaat gtggtggtgc atatcaaatc
     5521 ggaactacag ccaatgaaga tctagagaaa caagggtgtc atccttttta tgaatctgtc
     5581 atctcaaatc cttttgtcgc tgagtctgaa gggacctatg acacctatca gcatgttcca
     5641 gtggaaagct ttgcagaagt attgctgaga actggaaaat tggcagaggc taaaaataaa
     5701 ggagaagtat ttccaacaac tgaagttctc ttgcaactag caagtgaagc cttgccaaat
     5761 gacatgacct tggctcttgc ttaccttctt gccttaccac aagtgttaga tgctaaccgg
     5821 tgctttgaaa agcagtcccc ctctgcatta tctctccagc tggcagcgta ttactatagc
     5881 ctccagatct atgcccgatt ggccccatgt ttcagggaca agtgccatcc tctttacagg
     5941 gctgatccca aagaactaat caagatggtc accaggcatg tgactcgaca tgagcacgaa
     6001 gcctggcctg aagaccttat ttcactgacc aagcagttac actgctacaa tgaacgtctc
     6061 ctggatttca ctcaggcgca gatccttcag ggccttcgga agggtgtgga cgtgcagcgg
     6121 tttactgcag atgaccagta taaaagggaa actatccttg gtctggcaga aactctagag
     6181 gaaagcgtct acagcattgc tatttctctg gcacaacgtt acagtgtctc ccgctgggaa
     6241 gtttttatga cccatttgga gttcctcttc acggacagtg gtttgtccac actagaaatt
     6301 gaaaatagag cccaagacct tcatctcttt gagactttga agactgatcc agaagccttt
     6361 caccagcaca tggtcaagta tatttaccct actattggtg gctttgatca cgaaaggctg
     6421 cagtattatt tcactcttct ggaaaactgt ggctgtgcag atttggggaa ctgtgccatt
     6481 aaaccagaaa cccacattcg actgctgaag aagtttaagg ttgttgcatc aggtcttaat
     6541 tacaaaaagc tgacagatga aaacatgagt cctcttgaag cattggagcc agttctttca
     6601 agtcaaaata tcttgtctat ttccaaactt gttcccaaaa tccctgaaaa ggatggacag
     6661 atgctttccc caagctctct gtacaccatc tggttacaga agttgttctg gactggagac
     6721 cctcatctca ttaaacaagt cccaggctct tcaccggagt ggcttcatgc ctatgatgtc
     6781 tgcatgaagt actttgatcg tctccaccca ggtgacctca tcactgtggt agatgcagtt
     6841 acattttctc caaaagctgt gaccaagctg tctgtggaag cccgtaaaga gatgactaga
     6901 aaggctatta agacagtcaa acattttatt gagaagccaa ggaaaagaaa ctcagaagac
     6961 gaagctcaag aagctaagga ttctaaagtt acctatgcag atactttgaa tcatctggag
     7021 aaatcacttg cccacctgga aaccctgagc cacagcttca tcctttctct gaagaatagt
     7081 gagcaggaaa cactgcaaaa atacagtcac ctctatgatc tgtcccgatc agaaaaagag
     7141 aaacttcatg atgaagctgt ggctatttgt ttagatggtc agcctctagc aatgattcag
     7201 cagctgctag aggtggcagt tggccctctt gacatctcac ccaaggatat agtgcagagt
     7261 gcaatcatga aaataatttc tgcattgagt ggtggcagtg ctgaccttgg tgggccaagg
     7321 gacccactga aggtcctgga aggtgttgtt gcagcagtcc acgccagtgt ggacaagggt
     7381 gaggagctgg tttcacctga ggacctgctg gagtggctgc ggcctttctg tgctgatgac
     7441 gcctggccgg tgcggccccg cattcacgtg ctgcagattt tggggcaatc atttcacctg
     7501 actgaggagg acagcaagct cctcgtgttc tttagaactg aagccattct caaagcctcc
     7561 tggccccaga gacaggtaga catagctgac attgagaatg aagagaaccg ctactgtcta
     7621 ttcatggaac tcctggaatc tagtcaccac gaggctgaat ttcagcactt ggttttactt
     7681 ttgcaagctt ggccacctat gaaaagtgaa tatgtcataa ccaataatcc atgggtgaga
     7741 ctagctacag tgatgctaac cagatgtacg atggagaaca aggaaggatt ggggaatgaa
     7801 gttttgaaaa tgtgtcgctc tttgtataac accaagcaga tgctgcctgc agagggtgtg
     7861 aaggagctgt gtctgctgct gcttaaccag tccctcctgc ttccatctct gaaacttctc
     7921 ctcgagagcc gagatgagca tctgcacgag atggcactgg agcaaatcac ggcagtcact
     7981 acggtgaatg attccaattg tgaccaagaa cttctttccc tgctcctgga tgccaagctg
     8041 ctggtgaagt gtgtctccac tcccttctat ccacgtattg ttgaccacct cttggctagc
     8101 ctccagcaag ggcgctggga tgcagaggag ctgggcagac acctgcggga ggccggccat
     8161 gaagccgaag ccgggtctct ccttctggcc gtgaggggga ctcaccaggc cttcagaacc
     8221 ttcagtacag ccctccgcgc agcacagcac tgggtgtgag cggccgccta gagggcccta
     8281 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt
     8341 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     8401 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     8461 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     8521 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     8581 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     8641 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     8701 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     8761 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     8821 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     8881 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     8941 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     9001 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     9061 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9121 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9181 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9241 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9301 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9361 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     9421 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     9481 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     9541 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     9601 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     9661 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     9721 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     9781 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     9841 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     9901 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     9961 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
    10021 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    10081 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    10141 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    10201 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
    10261 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    10321 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    10381 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
    10441 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    10501 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
    10561 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    10621 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    10681 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    10741 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    10801 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    10861 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    10921 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    10981 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11041 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11101 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11161 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11221 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11281 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11341 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11401 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11461 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11521 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    11581 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    11641 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    11701 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    11761 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    11821 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    11881 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    11941 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    12001 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    12061 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    12121 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    12181 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    12241 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    12301 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    12361 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    12421 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12481 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12541 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    12601 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    12661 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    12721 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
    12781 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
    12841 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
    12901 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
    12961 gtt
//
