LOCUS       sequence-430424-       11871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1-V1251R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11871)
  AUTHORS   Petit AP, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, 
            Izaurralde E, Weichenrieder O
  TITLE     The structural basis for the interaction between the CAF1 nuclease 
            and the NOT1 scaffold of the human CCR4-NOT deadenylase complex.
  JOURNAL   Nucleic Acids Res. 2012 Nov;40(21):11058-72. doi: 
            10.1093/nar/gks883. Epub 2012 Sep 12.
  PUBMED    22977175
REFERENCE   2  (bases 1 to 11871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks883"; journalName: "Nucleic Acids Res"; date: 
            "2012-11"; volume: "40"; issue: "21"; pages: "11058-72"
COMMENT     Sequence Label: pT7-EGFP-C1-HsNot1iso1-V1251R_R
FEATURES             Location/Qualifiers
     source          1..11871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     553..572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        553..571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(650..671)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(911..930)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1258..1279
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8599..8720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8636..8655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8690..8709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8727..9182)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8864..8885)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9076..9095
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9209..9313
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9315..9672
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9315..9506
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9338..9358)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9523..9658
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9707..10501
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9707..10501
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9761..9780)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10371..10390
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10689..10708)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10733..10780
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11109..11697
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11598..11617
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      541 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      601 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      661 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      721 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      781 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      841 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      901 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      961 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1021 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1081 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1141 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1201 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1261 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1321 gtccggactc agatctcgag ctatgaatct tgactcgctc tcgctggcct tgtctcaaat
     1381 cagctacctg gtggacaatt taaccaagaa aaattaccga gccagccagc aggaaataca
     1441 gcatattgtg aatcggcacg gtcctgaggc agacaggcat ttattacgct gcctattttc
     1501 gcatgtggat ttcagtggcg atggtaaaag cagtggcaaa gatttccatc agactcagtt
     1561 tctgattcag gagtgtgcgt tgctgattac aaagccaaat tttatctcga cgctgtccta
     1621 tgccattgat aatccattgc actatcagaa gagtttaaag cctgcacccc acttatttgc
     1681 ccagctgagt aaagtgctca aattaagcaa agtacaagag gtaatttttg gccttgccct
     1741 gttgaattct tccagctcag atcttagagg tttcgctgcc cagtttatca aacagaagct
     1801 tccagatctt ctgcgttctt acattgacgc agacgtcagt ggaaatcaag aaggtggctt
     1861 ccaagatata gcaatagagg tcctacacct cctcctctcc catctcctct ttgggcagaa
     1921 gggagccttt ggagttggac aagaacagat agacgctttt cttaagacgc tgcgcagaga
     1981 ttttccccaa gaacgctgtc ccgtggtgct cgcaccactt ttataccctg aaaaacggga
     2041 cattctaatg gacaggatcc tgcctgattc cggaggggta gctaaaacca tgatggagag
     2101 ctctttggct gatttcatgc aagaagtagg ctatggcttt tgtgcaagta ttgaagaatg
     2161 tcgcaatata atcgtgcagt ttggtgttcg ggaggtcaca gctgcccagg ttgcaagggt
     2221 tttgggaatg atggctcgaa ctcattcagg attaacagat ggcattccat tacagagtat
     2281 ttctgctccg ggcagtggga tctggagtga tgggaaagat aaaagtgatg gagcacaggc
     2341 acacacatgg aatgtagaag tcttgattga cgttcttaaa gaactgaatc caagtttgaa
     2401 tttcaaggaa gtaacttatg aactggacca tcctggattt caaattcgtg acagtaaagg
     2461 acttcataat gtggtttatg gcattcagag gggtttgggt atggaagtgt tcccagtaga
     2521 cctcatatat agaccttgga aacatgctga aggccagctc tccttcattc aacattccct
     2581 tataaatcca gagatcttct gttttgctga ctatccctgt catactgttg ccactgatat
     2641 tctgaaagca ccaccagagg atgacaatcg agaaattgcc acatggaaga gcttggattt
     2701 gattgaatct ctgctgaggc ttgcagaggt tgggcagtat gagcaagtca aacagctctt
     2761 cagcttccct atcaaacact gtccagacat gctggtattg gccttactac aaattaacac
     2821 ctcttggcat accttgcgcc atgaacttat ctccactctg atgccaattt tccttggaaa
     2881 ccatcctaac tcagctatta ttttgcacta tgcatggcat gggcagggac agtctccctc
     2941 aattcgccaa cttatcatgc atgcaatggc agaatggtac atgagagggg agcagtatga
     3001 tcaggccaaa ttgtctcgaa tacttgatgt ggcccaggac ttgaaggcct tgtcaatgct
     3061 gctaaatggt actccatttg cctttgttat tgaccttgct gcacttgctt cacgtcgtga
     3121 atacctcaaa cttgataagt ggctcacaga taaaattcga gagcatgggg agccttttat
     3181 ccaggcgtgt atgacttttt taaagagacg gtgtccttct attttgggcg gacttgcccc
     3241 agaaaaagac cagcccaaaa gtgctcaact tcctccagaa actttggcga caatgttggc
     3301 ctgtctgcaa gcttgtgcag ggagtgtttc tcaggagcta tcagaaacta tcctcaccat
     3361 ggtagccaat tgcagtaatg ttatgaataa ggccagacaa ccaccacctg gagttatgcc
     3421 aaaaggacgt cctcctagtg ctagcagctt agatgccatt tctcctgttc agattgaccc
     3481 tcttgctgga atgacatctc ttagtatagg tggttcagct gcccctcaca cccagagtat
     3541 gcagggtttt cctccaaatt tgggttctgc attcagtacc cctcagtcac cagcaaaagc
     3601 atttccaccc ctttcaaccc ccaatcagac cactgcattc agtggtattg gaggactttc
     3661 atcacagctt ccagtaggtg gtcttggcac aggcagcctg actggtatag gaactggtgc
     3721 tcttggactc cctgcagtga ataacgaccc ttttgtacag aggaaactgg gcacctctgg
     3781 actgaatcag cctacattcc agcagacgga cttgtctcag gtgtggccag aggcaaacca
     3841 gcactttagt aaagagatag atgatgaagc aaacagctat ttccagcgaa tatataatca
     3901 tccaccacat ccaaccatgt ctgttgatga ggtattagaa atgctgcaga gatttaaaga
     3961 ctctactata aagagggaac gagaagtatt taactgtatg ctaaggaact tgtttgaaga
     4021 atatcgtttt tttccccagt atcctgataa agagttacat ataacagcct gcctatttgg
     4081 tggtataatt gagaaaggac tggtcactta catggcacta ggtctggctc tacgatatgt
     4141 tcttgaagcc ttacgcaagc cttttggatc caaaatgtat tatttcggga ttgctgcact
     4201 agatagattt aaaaacagat tgaaggacta tccccagtat tgtcagcatt tggcttctat
     4261 cagtcacttt atgcaatttc cacatcattt acaggagtat attgagtatg gacagcagtc
     4321 tagagatcct cctgtgaaaa tgcaaggctc tatcacaacc cctggaagta ttgcactggc
     4381 tcaggcccag gctcaggccc aggttccagc aaaagctcct cttgctggtc aagttagcac
     4441 tatggtaacc acctcaacaa ctaccactgt tgctaaaacg gttacggtca ccaggccaac
     4501 tggagtcagc tttaagaaag atgtgccacc ttctattaat actacaaata tagatacgtt
     4561 gcttgtggcc acagatcaaa ctgagagaat tgtggagccc ccagaaaata tccaggagaa
     4621 aattgctttt attttcaata atctctcaca gtcaaatatg acacaaaagg ttgaagagct
     4681 aaaggaaacg gtgaaagaag aatttatgcc ttgggtttca cagtatctgg ttatgaagag
     4741 agtcagtatt gagccaaact ttcatagcct gtattcaaac ttccttgaca cgctgaagaa
     4801 tcctgaattt aacaagatgg ttctgaatga gacctacaga aacattaaag tgctcctgac
     4861 ctctgataaa gctgcagcca atttctcaga tcgttctttg ctgaagaact tgggacattg
     4921 gctaggaatg atcacattag ctaaaaacaa acccatctta cacactgact tggatgtgaa
     4981 atcattgctg ctagaggctt atgttaaagg acaacaagaa ttgctctatg tagtgccctt
     5041 tgttgccaaa gtcttagaat ctagcattcg tagtgtgcgt tttaggccac caaacccttg
     5101 gacaatggca attatgaatg tattagctga gctacatcag gagcatgact taaagttaaa
     5161 cttgaagttt gaaatcgagg ttctctgcaa gaaccttgca ttagacatca atgagctaaa
     5221 acctggaaac ctcctaaagg ataaagatcg cctgaagaat ttagatgagc aactctctgc
     5281 tccaaagaaa gatgtcaagc agccagaaga actccctccc atcacaacca caacaacttc
     5341 tactacacca gctaccaaca ccacttgtac agccacggtt ccaccacagc cacagtacag
     5401 ctaccacgac atcaatgtct attcccttgc gggcttggca ccacacatta ctctaaatcc
     5461 aacaattccc ttgtttcagg cccatccaca gttgaagcag tgtgtgcgtc aggcaattga
     5521 acgggctgtc caggagctgg tccatcctgt ggtggatcga tcaattaaga ttgccatgac
     5581 tacttgtgag caaatagtca ggaaggattt tgccctggat tcggaggaat ctcgaatgcg
     5641 aatagcagct catcacatga tgcgtaactt gacagctgga atggctatga ttacatgcag
     5701 ggaacctttg ctcatgagca tatctaccaa cttaaaaaac agttttgcct cagcccttcg
     5761 tactgcttcc ccacaacaaa gagaaatgat ggatcaggca gctgctcaat tagctcagga
     5821 caattgtgag ttggcttgct gttttattca gaagactgca gtagaaaaag caggccctga
     5881 gatggacaag agattagcaa ctgaatttga gctgagaaaa catgctaggc aagaaggacg
     5941 cagatactgt gatcctgttg ttttaacata tcaagctgaa cggatgccag agcaaatcag
     6001 gctgaaagtt ggtggtgtgg acccaaagca gttggctgtt tacgaagagt ttgcacgcaa
     6061 tgttcctggc ttcttaccta caaatgactt aagtcagccc acgggatttt tagcccagcc
     6121 catgaagcaa gcttgggcaa cagatgatgt agctcagatt tatgataagt gtattacaga
     6181 actggagcaa catctacatg ccatcccacc aactttggcc atgaaccctc aagctcaggc
     6241 tcttcgaagt ctcttggagg ttgtagtttt atctcgaaac tctcgggatg ccatagctgc
     6301 tcttggattg ctccaaaagg ctgtagaggg cttactagat gccacaagtg gtgctgatgc
     6361 tgaccttctg ctgcgctaca gggaatgcca cctcttggtc ctaaaagctc tgcaggatgg
     6421 ccgggcatat gggtctccat ggtgcaacaa acagatcaca aggtgcctaa ttgaatgtcg
     6481 agatgaatat aaatataatg tggaggctgt ggagctgcta attcgcaatc atttggttaa
     6541 tatgcagcag tatgatcttc acctagcgca gtcaatggag aatggcttaa actacatggc
     6601 tgtggcattt gctatgcagt tagtaaaaat cctgctggtg gatgaaagga gtgttgctca
     6661 tgttactgag gcagatctgt tccacaccat tgaaaccctc atgaggatta atgctcattc
     6721 cagaggcaat gctccagaag gattgcccca gctgatggaa gtagtgcgat ccaactatga
     6781 agcaatgatt gatcgtgctc atggaggccc aaactttatg atgcattctg ggatctctca
     6841 agcctcagag tatgatgacc ctccaggcct gagggagaag gcagagtatc ttctgaggga
     6901 atgggtgaat ctctaccatt cagcagcagc tggccgcgac agtaccaaag ctttctctgc
     6961 atttgttgga cagatgcacc agcaaggaat actgaagacc gatgatctca taacaaggtt
     7021 ctttcgtctg tgtactgaaa tgtgtgttga aatcagttac cgtgctcagg ctgagcagca
     7081 gcacaatcct gctgccaatc ccaccatgat ccgagccaag tgctatcaca acctggatgc
     7141 ctttgttcga ctcattgcac tgctcgtgaa acactcaggg gaggccacca acactgtcac
     7201 aaagattaat ctgctgaaca aggtccttgg tatagtagtg ggagttctcc ttcaggatca
     7261 tgatgttcgt cagagtgaat ttcagcaact tccctaccat cgaattttta tcatgcttct
     7321 cttggaactc aatgcacctg agcatgtgtt ggaaaccatt aatttccaga cacttacagc
     7381 tttctgcaat acattccaca tcttgaggcc taccaaagct cctggctttg tatatgcctg
     7441 gcttgaactg atttcccatc ggatatttat tgcaagaatg ctggcacata cgccacagca
     7501 gaaggggtgg cctatgtatg cacagctact gattgattta ttcaaatatt tagcgccttt
     7561 ccttagaaat gtggaactca ccaaacctat gcaaatcctc tacaagggca ctttaagagt
     7621 gctgctggtt cttttgcatg atttcccaga gttcctttgt gattaccatt atgggttctg
     7681 tgatgtgatc ccacctaatt gtatccagtt aagaaatttg atcctgagtg cctttccaag
     7741 aaacatgagg ctccccgacc cattcactcc taatctaaag gtggacatgt tgagtgaaat
     7801 taacattgct ccccggattc tcaccaattt cactggagta atgccacctc agttcaaaaa
     7861 ggatttggat tcctatctta aaactcgatc accagtcact ttcctgtctg atctgcgcag
     7921 caacctacag gtatccaatg aacctgggaa tcgctacaac ctccagctca tcaatgcact
     7981 ggtgctctat gtcgggactc aggccattgc gcacatccac aacaagggca gcacaccttc
     8041 aatgagcacc atcactcact cagcacacat ggatatcttc cagaatttgg ctgtggactt
     8101 ggacactgag ggtcgctatc tctttttgaa tgcaattgca aatcagctcc ggtacccaaa
     8161 tagccacact cactacttca gttgcaccat gctgtacctt tttgcagagg ccaatacgga
     8221 agccatccaa gaacagatca caagagttct cttggaacgg ttgattgtaa ataggccaca
     8281 tccttggggt cttcttatta ccttcattga gctgattaaa aacccagcgt ttaagttctg
     8341 gaaccatgaa tttgtacact gtgccccaga aatcgaaaag ttattccagt cggtcgcaca
     8401 gtgctgcatg ggacagaagc aggcccagca agtaatggaa gggacaggtg ccagttagcc
     8461 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     8521 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     8581 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8641 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8701 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     8761 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8821 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     8881 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     8941 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     9001 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     9061 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     9121 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     9181 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9241 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9301 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     9361 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9421 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9481 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9541 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9601 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9661 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     9721 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     9781 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     9841 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     9901 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     9961 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    10021 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    10081 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    10141 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    10201 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
    10261 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
    10321 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
    10381 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    10441 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    10501 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
    10561 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    10621 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
    10681 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
    10741 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
    10801 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
    10861 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
    10921 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
    10981 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    11041 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    11101 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    11161 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    11221 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    11281 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    11341 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11401 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11461 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11521 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11581 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11641 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11701 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    11761 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
    11821 atagtaatca attacggggt cattagttca tagcccatat atggagttcc g
//
