LOCUS       sequence-430429-       12097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a cDNA under 
            regulation of CaMV 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Dombey R, Buendia-Avila D, Barragan-Borrero V, Diezma-Navas L, 
            Ponce-Mane A, Vargas-Guerrero JM, Elias R, Mari-Ordonez A
  TITLE     Atypical epigenetic and small RNA control of degenerated transposons
            and their fragments in clonally reproducing Spirodela polyrhiza
  JOURNAL   bioRxiv 2024.04.03.587901; doi: 
            https://doi.org/10.1101/2024.04.03.587901
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.03.587901; doi: https://doi.org/10.1101/2024.04.03.587901"
COMMENT     Sequence Label: p35S-FHA-SpAGO4a_cDNA
FEATURES             Location/Qualifiers
     source          1..12097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    155..179
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    247..267
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        270..288
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     270..287
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        948..1292
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1236..1256
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1393..1417
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1423..1446
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1462..1488
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4142..4166)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4179..4188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4553..4732
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4774..5325
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      5345..5597
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5690..5714
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6235..7026
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7272..7860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7761..7780
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8014..8031
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8046..8186
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8112..8131)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8272..8294
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8530..8724
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8790..9863)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10292..10921)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tcaaagtact ttaaagtact ttaaagtact ttaaagtact ttgatcccga ggggaaccct
       61 gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
      121 tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa
      181 acactgatag tttaaactga aggcgggaaa cgacaatctg atccaagctc aagctaagct
      241 tggctccaac tttgtataga aaagttgtca tttaggtgac actatagaat actcaagcta
      301 tgcatccaac gcgttgggag ctctcccata tggtcgacta gagccaagct gatctccttt
      361 gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag aaagttcgac
      421 ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct cttcaatttc
      481 agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct catcaagacg
      541 atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt taaagatgca
      601 gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc
      661 agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag gcaagtaata
      721 gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga gtcaaaaatt
      781 cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat acagagtctt
      841 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cactctcgtc
      901 tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
      961 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
     1021 aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag
     1081 gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc acccacgagg
     1141 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
     1201 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
     1261 atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca attaccacaa
     1321 caaaacaaac aacaaacaac attacaattt actattctag tcgacctgca ggcggccgca
     1381 ctagtgatat cagcaagttt gtacaaaaaa gcaggcttca tggattacaa ggatgacgat
     1441 gacaagggag gtggcggatc ctacccatac gatgttccag attacgctgg aggcggtcta
     1501 gtgcccatga aaagaccagg aacgggaaag aatggacgga ccctttcatt gctcaccaat
     1561 catttcagag ttgatatgcg taacgttgat gatggacatt tctaccacta cagtgtgttg
     1621 ctcaagtatg atgatgatcg ccctgtggag gggaagggaa ttggacgaag ggtaattgat
     1681 aagttgcacg agatatatga tgctgaactt ggtggcaagg attttgccta tgatggtgag
     1741 aaaagcttgt tcaccattgg ctctctgccc caggtgaaca ataattttac tgtagtcctg
     1801 gaggatgttt catcaaatag aactggggaa gtttcgaacg gaagctctgg tggaaatgat
     1861 agcctggagg atagtgaaag aaagaggagg cgacggccct atcaaatgaa gacttttaag
     1921 gttgagctga gcttctctgc aaaggttgcg atccagtcca ttgcaaatgc tcttcgagga
     1981 cacgaaaatg agaattctca ggaagcactc agggtgcttg atatcatact aaggcagcat
     2041 gccgcgaagc aggggtgcct gcttgttcgg caatcatttt ttcataatga tcctaagaat
     2101 tatgtcgaat tgggaggagg agtttcagga tgcaggggtt ttcattcgag ctttcggaca
     2161 acccagggtg gtctgtccct gaacatggat gtctcaacta caatggttgt gaagccgggt
     2221 ccagtcatag attttctcat ggcaaaccaa aaggctcgta acgctcgaga aattgactgg
     2281 ctgaaggcca aacgccagct gaaaaatctt agggtaaaaa ttacttccac aaacttggag
     2341 tttaaagttg cgggattgag cgatcagcca tgtaaccaac tcaagttttc actgaaacag
     2401 aagaatcgtg gaaatgggag tggtgattct gatgtcgaag ccgttgatat aacagtgtat
     2461 gaatattttg tcaaccaccg taacataaag ttgacaaagt ctgctgatct gccttgtgtt
     2521 aatgttggga aatcaaaacg tcccacatat tatccattgg agctatgttc tcttgtgtcg
     2581 ctgcaacgtt atacaaaggc actgtcgaca ttccaacgat cctcacttgt agagaaatct
     2641 cggcagaatc cccaagagag gatgagagtt ttaaaagatt cattgaagat gagtcgctat
     2701 gatgctgata ggatgctgaa tgcatgcggc atttctattt gtccggaatt tatgcaggtt
     2761 gaaggtcgag tattacaaac accgaagctt agagtaggta atggtgaaga ctttatacca
     2821 cggaatgggc ggtggaattt caacgacaag acatttgcag aacccacagc catcgataaa
     2881 tgggctgtcg tcaatttttc tgttcgctgt gatgtaggcc attttattcg ggaaatgaaa
     2941 aaatgtgggc agatgaaagg aatcaatatt gcgcatccat ttgatgtgtt tgaggaggat
     3001 gcttcattta ggcgagctcc tccaaatgtt agggtggacg aaatgtttga gaaaatgagg
     3061 aaaaagcttc ctgggattcc tcgctttctt ctgtgtattc ttccagtgag gaagaattct
     3121 gatctctatg gtccatggaa gcgcaagaac cttaccgaat ttggtattgt tacccagtgt
     3181 atcggtcccc ccactaaaat cagtgatcag tattttacaa atcttcttat gaagatcaac
     3241 agcaaattgg ggggcatcaa ttctttgtta caggtggagc ttccgcattc aagcactctt
     3301 gttcccaagg tttcgaccat catccttggg atggacgttt cacatggtcc acctggccaa
     3361 tctgatatcc catcagttgc cgcagttgtc ggttcgcggc agtggccttc aatatctcgt
     3421 tacagagcgg aagtgcgcac tcagtccccc aaggttgaga tgatagattc tttatttaag
     3481 cctgaaccag ataatcatga ttcgggcata attcgggaac tcttggtgga cttttatgcc
     3541 acttctcagc aaacgaaacc tgatcgggtg ataatttttc gggatggcgt gagcgaatcg
     3601 cagttcaacc aggtgctgaa cattgagctg gatcaaatca tccaggcctg caagctcctc
     3661 gacgacacgt ggcatccgag gttcactgtg attgtagccc agaagaacca tcacaccaag
     3721 ttctttcagg ccaattccac gcataatgtt cctgccggta cggtggtcga caccagagtg
     3781 tgccatccac gaaactttga cttttacatg tgctcgcatg ccgggatgat cggtacgacc
     3841 cgcccgactc actatcatgt gctgtatgat gagattggct tctcagcgga cgaactccaa
     3901 gaactcgttc attctctctc ctatgtgtac caacggagca cgaccgccgt ttccctggtt
     3961 gctccaatat gctatgccca cttggcggcg gcccaggtgg gacaattcat caagttcgag
     4021 gagtcctcgg agacctcatc cggccgcggc ggcgtgacct ccgccggcag cgtccccgtg
     4081 gtggcgctgc cgaggcttca cccccaggtg agcagctcca tgttcttctg ctgaactaga
     4141 tacccagctt tcttgtacaa agtggtgata tcccgcggcc atggcggccg ggagcatgcg
     4201 acgtcgatct aactgactag ccgcggccat gctagagtcc gcaaaaatca ccagtctctc
     4261 tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa
     4321 gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta
     4381 tgtatttgta tttgtaaaat acttctatca ataaaatttc taatccctaa aaccaaaatc
     4441 cagtgacctc ccgggtaccg cgaattatcg atcatgagcg gagaattaag ggagtcacgt
     4501 tatgaccccc gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca
     4561 acgttgaagg agccactgag ccgcgggttt ctggagttta atgagctaag cacatacgtc
     4621 agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag caaatatttc
     4681 ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat tagagtctca
     4741 tattcactct caactcgatc gaggggatct accatgagcc cagaacgacg cccggccgac
     4801 atccgccgtg ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa ccactacatc
     4861 gagacaagca cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac
     4921 ctcgtccgtc tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc
     4981 ggcatcgcct acgcgggtcc ctggaaggca cgcaacgcct acgactggac ggccgagtcg
     5041 accgtgtacg tctccccccg ccaccagcgg acgggactgg gctccacgct ctacacccac
     5101 ctgctgaagt ccctggaggc acagggcttc aagagcgtgg tcgctgtcat cgggctgccc
     5161 aacgacccga gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg
     5221 gcggccggct tcaagcacgg gaactggcat gacgtgggtt tctggcagct ggacttcagc
     5281 ctgccggtgc cgccccgtcc ggtcctgccc gtcaccgaaa tctgatgacc cctagagtca
     5341 agcagatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     5401 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     5461 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     5521 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     5581 atctatgtta ctagatcgac cggcatgcaa gctgataatt caattcggcg ttaattcagt
     5641 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
     5701 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
     5761 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
     5821 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
     5881 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
     5941 tgcctgtgat caattcgggc acgaacccag tggacataag cctgttcggt tcgtaagctg
     6001 taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt
     6061 ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct
     6121 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     6181 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgggg
     6241 gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc
     6301 catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg
     6361 aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg
     6421 cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt
     6481 ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca
     6541 gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc
     6601 gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc
     6661 gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta
     6721 tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat
     6781 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc
     6841 gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc
     6901 gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc
     6961 gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc
     7021 aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     7081 aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt caagtctgct
     7141 tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat atcatgcatg
     7201 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     7261 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     7321 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     7381 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     7441 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     7501 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7561 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7621 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7681 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7741 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7801 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7861 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7921 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7981 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     8041 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     8101 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     8161 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     8221 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     8281 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
     8341 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
     8401 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
     8461 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg
     8521 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
     8581 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
     8641 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
     8701 ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg
     8761 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
     8821 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
     8881 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
     8941 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
     9001 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
     9061 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
     9121 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
     9181 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
     9241 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
     9301 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
     9361 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
     9421 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
     9481 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
     9541 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
     9601 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
     9661 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
     9721 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
     9781 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
     9841 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
     9901 agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
     9961 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
    10021 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
    10081 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
    10141 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
    10201 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
    10261 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
    10321 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
    10381 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
    10441 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
    10501 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
    10561 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
    10621 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
    10681 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
    10741 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
    10801 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
    10861 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
    10921 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
    10981 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
    11041 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
    11101 atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
    11161 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
    11221 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
    11281 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
    11341 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
    11401 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
    11461 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
    11521 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
    11581 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
    11641 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
    11701 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
    11761 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
    11821 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
    11881 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
    11941 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
    12001 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt
    12061 cagaagccga ctgcactata gcagcggagg ggttgga
//
