LOCUS       sequence-430448-       11849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A simplified disease resistance assay using YFP-expressing Potato 
            Virus X in N. benthamiana .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11849)
  AUTHORS   Hasegawa K, Timmers T, Chai J, Maekawa T
  TITLE     A disease resistance assay in Nicotiana benthamiana reveals the 
            immune function of Response to HopBA1.
  JOURNAL   Plant Physiol. 2024 Jul 8:kiae368. doi: 10.1093/plphys/kiae368.
  PUBMED    38976586
REFERENCE   2  (bases 1 to 11849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2024 Jul 8:kiae368. doi: 10.1093/plphys/kiae368."
COMMENT     Sequence Label: pSfinx-mYFP
FEATURES             Location/Qualifiers
     source          1..11849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      365..800
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    951..975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     1080..1097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1214..1235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1250..1255,1256..1273,1274..1280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1288..1304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1293..1315
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1312..1328
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1312..1328
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1347..1367
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1349..1367
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1519..1541
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1533..1550
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1534..1550
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(1559..1811)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(2699..3412,3413..3415,3416..3418))
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2744..2765)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3093..3112
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3352..3373
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8397..8406
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9112..9457)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9149..9169)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    9554..9576
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     rep_origin      complement(9643..10231)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9723..9742)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10332..11147)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(10332..11147)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     11055..11074
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     11139..11158
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     mobile_element  join(11241..11849,1..159)
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
ORIGIN
        1 tatacgcagc gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga
       61 cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat
      121 ctgaacataa aacactatca ataagttgga gtcattacca gattttgaga cacaacgtgg
      181 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
      241 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
      301 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcg
      361 atccccatcc aacagcccgc cgtcgagcgg gcttttttat ccccggaagc ctgtggatag
      421 agggtagtta tccacgtgaa accgctaatg ccccgcaaag ccttgattca cggggctttc
      481 cggcccgctc caaaaactat ccacgtgaaa tcgctaatca gggtacgtga aatcgctaat
      541 cggagtacgt gaaatcgcta ataaggtcac gtgaaatcgc taatcaaaaa ggcacgtgag
      601 aacgctaata gccctttcag atcaacagct tgcaaacacc cctcgctccg gcaagtagtt
      661 acagcaagta gtatgttcaa ttagcttttc aattatgaat atatatatca attattggtc
      721 gcccttggct tgtggacaat gcgctacgcg caccggctcc gcccgtggac aaccgcaagc
      781 ggttgcccac cgtcgagcgc ctttgcccac aacccggcgg ccggccgcaa cagatcgttt
      841 tataaatttt tttttttgaa aaagaaaaag cccgaaaggc ggcaacctct cgggcttctg
      901 gatttccgat ccccggaatt agatctcaaa caaacacata cagcgactta gtttacccgc
      961 caatatatcc tgtcaaggcc ttcatgttct ttcctgcgtt atcccctgat tctgtggata
     1021 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1081 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1141 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     1201 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     1261 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     1321 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     1381 agctcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     1441 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     1501 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcccga
     1561 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
     1621 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
     1681 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     1741 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     1801 tttgaacgat cggggaaatt cgcggtaccc actagttttt tttttttatt tatattattc
     1861 atacaatcaa accagaaaat actatgaaac tggggtaggc gtcggttatg tagacgtagt
     1921 tatggtggtg gtagagtgac aacagcctca gcggttgttg ttccagtgat acgacctcga
     1981 gtgacagctg catctaggct ggcaaagtcg ttggattgtg ccctggcctt tgtaatcttc
     2041 acaaaggcag cagtttgggc agcattcatt tcagcttcag acggtggccg gatgagcccc
     2101 tctttgggca tgatggcagc tgggttggtg actccattga agaagtcgaa tgcagcgaat
     2161 ttgtgctcag gcttgaaacc ttgtgcttgc cagttagcag gtggactgtt gttagttaac
     2221 atccagttcc ataccactgg ggcatacttc atgcaaaatt gcctaagtgt gcacacctct
     2281 ttaattgctg ctgccagtct agctctgctg atgccgttgg aatagggacc tgtatctatc
     2341 atttctgttt gagcggatga tcctacatca gcacagtgtc tgactaagtc ccaagcagcc
     2401 tgtgccatag tgtctgtggg caccttcatg tccttccaaa tagcctcaat cttgctgagg
     2461 tcctcatttg ttgcgacagc attgctggct actatggcac gggctgtact aaagaaatcc
     2521 ccatccggga tagtgaacag gcctgaagct gtggcaggag ttgcgcctgc agtttttgtg
     2581 gtagttgagg tagttgaccc tatgggctgt gttgtgctag ctggtgctga catctttcga
     2641 gtatcaatgg aaacttaacc gttcatcggc ggtcgacgcg gccgcggccg aggcggcctt
     2701 acttgtacag ctcgtccatg ccgtgagtga tcccggcggc ggtcacgaac tccagcagga
     2761 ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactggtag ctcaggtagt
     2821 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     2881 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     2941 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     3001 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     3061 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     3121 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     3181 tcatgtggtc ggggtagcgg gcgaagcact tcaggccgta gccgaaggtg gtcacgaggg
     3241 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     3301 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     3361 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
     3421 ccgtaatggc cggcgcgcca atcgatgcta gctggtgctg acctctttcg agtatcaatg
     3481 gaaacttaac cgttcaacgg agagtggctt gagatcggca atggccctta tggtttctgc
     3541 atctagtttg caagccaaca ctgtgattga ttccccagta atcttgatga cacaaggttc
     3601 agtcctcact aaggaagtgc taatgactgc tatgattgtt accacaagca caagtatgat
     3661 tgcgttgaga tatgtattta cttccataga tcagcaaagt cagtagcaaa acggcagcaa
     3721 atgctgcgtt ctttccgttg tgtagactca ctcgtgaccc tagatttggg gagttgtaca
     3781 agattgcttt ggtgccgtct ctgtaagctc ctccgtgtgg caagctgtga atgttgtcac
     3841 cgacgtgggg taagctattt ctagaaagca gaaaggtaat tgaaactaaa gcaaatgata
     3901 gacctaatac tatgtacact ttttcagaat tgaccggagc ggtcagccta tggccctgcg
     3961 cggacatatg tcaatccctt tgacctggtg atagcgttgt agaaagctgt ggactggcca
     4021 atttcctcta ttggtgcggc agacacaata gtgactactt tgaactcaag tcccgtcact
     4081 tggcagggct taacaaactc aacaccatgc ctggacagtg ttgtctcaga ctcctcatca
     4141 atggctatca cctttccgag taggggccct ttgaatatgc cagtgatctc taagtgccct
     4201 tcttccggtg agttggtctc gaaatcgaag ccacagccag ctatcaaatc tgccactttc
     4261 ctcggaactc gaaatgatgt ttccaagtag aagtggggct ctaggctaaa ctccggtgcc
     4321 tgataagggt cagcaaaaag tgcctggtat gagttccttg tggtgttgtc caaagtatac
     4381 tcatcgagga ttgcgaagtt gccctcagga ataggtcctg gcttctgtat gcctctagtt
     4441 ctgatactca ccttgtcagg gacaccgagt gtatgcacgg tgaatgttgg gtgtctgagg
     4501 atcaacttcc ttagggctgt ggacttaccg gctccggcta ctgcatgtac taccaaaggt
     4561 cctgaatcta aagatttgga agtcctagaa taacctaaac ttttcaaact actgatgaga
     4621 atatccatct tattcaaatc tctaaggtaa cttaacggtt aaagaaagtt tctgaggcgg
     4681 gaaagtgaga cagtgcctct ccctgacttg attagtgttc tgcaagtgag tgtgtgtgcc
     4741 tgacactgtt tctcatcgaa caagtcatgc agagagtcct tgtggtcata ggcataactc
     4801 agatcaattt cataggaatc ttgacatttc ttgagttcac ccttagcttc agccaatttt
     4861 aagctaacat ggagcttaat tgggtctttc atcacccctt ttggtgtgat cagccaacca
     4921 caaaactcag gccaactgcc cttcttttgc tgcgtgatta caggctttga ctttaggagt
     4981 aatttgtcct caagcctgtg gaaactatgc ttcacttctg gaacacagtc cagtgcggag
     5041 tcgtctcctg cataaacttg agcagttccg gctgggatgt caaactttgt atgggtgtaa
     5101 gctatgttgc actcagtgtt tgcatcaaaa gtgggacctt caccagtcag gcgcataatt
     5161 gataacgtgc ctaggaaaat ctgtgcatta gtcttaatat ctatgtatgc ctgaatgatt
     5221 tcctctggta tgcagtggtg tttggctttg agcacctcaa attgcaacat ggctccatcc
     5281 tgagactggt cgaaagctgt gtagtcatta gccaagctag gtctgctgaa attccagttg
     5341 ttcaaggccc atgcagacat gtcatctggc gtggtctcac agtttatgaa gacttctttt
     5401 ggctggaaag cctgtctgaa ccatcgcatg tacctagcca tagttccaaa aagcatcaca
     5461 gtctgctggt aaaaggctgc tatggtttga cctggcttaa tcttgggtag acctagtttt
     5521 tccacctttg tgacccactg cgacttgagg aataccataa tcttattttc atcgaagtct
     5581 gggctctgtc tcacagtccc attgatcaag ttgcactttg acttgctgag gtacttgctt
     5641 tgtacttcgt gggcacaagc ttcccagacc tcttgggaaa aaggaatacg ctctttgggt
     5701 aaacccatag ctttttggta gtttaaaaac agaacgtccc caatgtcctt cttgctcagg
     5761 aattctcgga agtttgtctc ttgattgctg gtcttgagcc gcgcatctat agtcgcccag
     5821 aggagggtct catcttttgc ttgttgatgc gaaaacaact gaatggttgt gtcttcagtt
     5881 tggacacagt ttgaatgacc atgggattca gagtggatct ctctgtcaaa cttttccaag
     5941 agttcctctt tgtactcttc tagcacggac tcctcattct cgacacacat gtgtgtctgg
     6001 ggctcaggct cttgaattgg ctctgcctct gccggctcgt actccctgag tgcttgttct
     6061 ctcaccactg ataggaaagt cttgaggtaa ggggtgctgt ccaacttttc ccagaaggcc
     6121 gaggaatttg cacttgtgtt cacgaagtga atcctatcgg tggctctaga aagtgccgtg
     6181 tacatcacat tcgcgctaca cacttgggtg ttgtggtcca acactatttg taccttcggc
     6241 ttagttagcc cctggcatcc agcgtatgtg aacgtgtcat tcctcccggt gcccatgtac
     6301 agctttctct tctcatccga gggtaccaaa gttgggattc cttctaagaa ctcggcgctc
     6361 atgctgattt cggtgactcc cgttctctca ctgtagacac caagcatgtt cgcaagatct
     6421 ttcttgttgc ggtgtgtggc attgagatag tatcggcagt attttgagaa gtactctgtt
     6481 gctggtccca aatgcctgat ggaggcgtcc tcagcagttt catggtagac gctttgtctg
     6541 ctatctcctg ttagaatgat tagcttgatt ttagagtaga aacagactaa ggcttctatg
     6601 taaccgggag gaagttttga gtaatcgtca aagatgacta tgctgcctgt tcccccaatt
     6661 aacgcctttt cataggtctt gaacatatag ggctcagtgt taggcacttt cttactccaa
     6721 tctagccgca gttcattggt cggcaggact acagtgatgt ccgagccctt gccaatttct
     6781 ctcaatgcct tctggatggc atgacttttt ccagaacctc cagctccatg aatgacacaa
     6841 gtcgcaactt tcctctcagt caagctttct atctttcctg tcaagtattc gccccaactc
     6901 gttgcctgtg tctttgtgat ggcaccaatt ctcttgttct ttacatcact gccgtagtct
     6961 ctggctctaa gcaggtccaa agggatggtg gcagggcttc tgttcatagc gaacaattct
     7021 cgtgccaact cttttggagt tccttcaggg aagtcctctt tttccagccc ggagaccatt
     7081 tctgtgatgg gcatgatcat tgttccactc ctatccctct gaatttccag cgccttgaat
     7141 ccaacagcat ttaattgagg aatccagtgt ttccaaggaa gtccttcgat gacttgttgc
     7201 gtcgctgttt caacggtttc ttttccaagg ggtttaacct gctcctcttc cacactagaa
     7261 tctgtgaatg aagcatcttc ccaccttgca gggacaggtt tagtggttgg gagtgtgagt
     7321 tcttgtctgg catcattcgt gccaggaagt tcggcgttgc tgggtattgc ccccatgccc
     7381 ccgcttattt catttggttc ggcatcacct tcagctctca ttgactcctg gatttcagct
     7441 aggcgtttag ctttctttgc ggcctggtgt ttcacctccc tactgtgttt cctgtggagc
     7501 ttgttgagga aagtttctgc agggtttgag tattgatttc tgtctcctgc aggttcttta
     7561 acttcttgac cgacatccat ctcgggcgtt ttcctgattg cagctaaatc ttcctctgcg
     7621 tttaccgcat aagctggcat tcttgtgaag caatcaagca agtccccatc tgagaacaaa
     7681 ctttccattt cctctacatc cgacacttcc cttggtcgga aggctttcca cgcttgcaag
     7741 gggtggaatc tgaagtccca agtttccact ttgaaagaaa aatccaccgg gtggaaatca
     7801 actgctttga ctaacttcgc gtagtcctca agaccaaaca gctgcatctt tttctcttgt
     7861 atccaagccc tcaccggtgc aagggtcttt gttagcaagc caccagaaag tgtgtctgag
     7921 aagcaggtgg tagcttgtaa atcggcaagg aactccatgt agcttactaa gtaaaccagt
     7981 tccacagcag agtatttgtc caagtcagat gatttaatta attgtctgac tttggcaaaa
     8041 atgtcacaat tttttgcgac cttgactgtc ctaacataca agaagagctg catcatagtt
     8101 ttcttgagaa tcggcttctt gcagttgtga acttttggga ggaagatctg tggtggaatc
     8161 acgtacctgt caggttggcc gaaagtgcgc acctccggtg tgagcaagtc tcctctcctg
     8221 atgcagtaca agtggtttgc cgcgaacgat tcctgcaact gtactgtcac tgtgtgcatc
     8281 tcaacctgct cagtcgtgta attgagatgt ccgagaaagc tatccttggg gtccctccac
     8341 ttgatctttc ccactttgag ccattgtaga tgagcgaatt catgatggta tgccccgcca
     8401 ccatggttgc ctggtatata ctggaaacca tctccgaagt atttgaggct gtatatgttc
     8461 gggtgagtgc tttccatttt aaaggctgcc tcaacgggga gaactaaggt cgcatacaat
     8521 gtttgcaatt ttgggcagtt ttggaatgtc tccactatgt agctcggatc caagaagtgc
     8581 agagtgtcac taatgtatgc tgtttccgtt gtgatctctg tgagtttgtc aattattgtt
     8641 tccttggggt acctggctac gtctcttggt tcaatggcaa cattttggaa aatgtccttg
     8701 atccgcgggt ttcttctcat gtagtttagc tttctgggtt taagaaacat aaatgtaaca
     8761 ggttcttgtg gtaggatgga accaagcacc tctagaagtt tattctctat ggtcttagcg
     8821 gctgcatgtg tatgcaattc aatgctatag ggattggtgg ctatgccgaa cccctctaga
     8881 tcattagccg cttcaaccgt ttgagcgtaa gggttagcta gtttgtgttt ttccatgatg
     8941 gggcgaatgt ttctataagc ctcatcttgg atgagagttt ttgtggtgga gtctgtaaaa
     9001 gattggtaaa cctcgcgcac cttggccatt tgttaaactt tctttttcaa gcgggtgtgt
     9061 aacaattggg cgtggtgggt ttggttgtgt tggtggtgta tggtttagtt ttctcctctc
     9121 caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc
     9181 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
     9241 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
     9301 ggcagaggca tcttcaacga tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc
     9361 accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat ggaatccgag
     9421 gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt cttctgagac
     9481 tgtatctttg atatttttgg agtagacaag tgtgtcgtgc tccaccatgt tgacctgcag
     9541 gcatgcgaga acgttacacc acaatatatc ctgccaagat ctcatgtgag caaaaggcca
     9601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10081 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10201 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10261 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatggtta caaccaatta
    10321 accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
    10381 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
    10441 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
    10501 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
    10561 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
    10621 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
    10681 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
    10741 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
    10801 gaatcaggat attcttctaa tacctggaat gctgttttcc ctgggatcgc agtggtgagt
    10861 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
    10921 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
    10981 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcggtagat tgtcgcacct
    11041 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
    11101 tttaatcgcg gccttgagca agacgtttcc cgttgaatat ggctcataac accccttgta
    11161 ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca
    11221 atgtaacatc agagattttg ggtgatgctg ccaacttact gatttagtgt atgatggtgt
    11281 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
    11341 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
    11401 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
    11461 atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt
    11521 ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta acctcgcgca
    11581 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg
    11641 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg aagacggttg
    11701 ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg agcctgctgt
    11761 caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa tcccgcctga
    11821 agggaaagct gcacgtaatc agcaagcga
//
