LOCUS       sequence-43045-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Hartmann C, Tabin CJ
  TITLE     Wnt-14 plays a pivotal role in inducing synovial joint formation in 
            the developing appendicular skeleton.
  JOURNAL   Cell. 2001 Feb 9. 104(3):341-51.
  PUBMED    11239392
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2001 
            Feb 9. 104(3):341-51."
COMMENT     Sequence Label: pGEM cAgg (CT#689)
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(804..822)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(805..822)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(840..856)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(840..856)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(853..875)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    864..880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctccggccgc caggcggccg cgggaattcg attatacctg agcagtctcc cctgcgtgtt
       61 gtcctgggaa gctccctgaa catcccctgc tatttcaaca tcccagagga agaggacacc
      121 aatgctctgc tgaccccccg gatcaaatgg agcaagcttt ccaatggaac agagattgtc
      181 ttactagtgg ccaccggtgg gaagatccgg ctcaatgcag agtacagaga ggtgatctcc
      241 ttgcccaatt accctgccat ccccactgat gccaccttgg aaatcaaggc gctgagatcc
      301 aaccacactg ggatttatcg ctgtgaagtg atgtatggga ttgaggacag acaagacacc
      361 atagaggtcc tggtgaaagg cgtcgtgttc cactacagag caatctccac aaggtacact
      421 ttgaacttcg agagggcaaa gcaggcctgt atccagaaca gtgccgtcat tgccacccct
      481 gagcagctgc aggctgccta cgaggatggg tacgagcagt gcgatgccgg ctggctggct
      541 gaccagactg tcaggtaccc catccatctg ccccgggagc gctgctacgg tgacaaggat
      601 gagtttccag gagtgagaac ctacggtgtc cgtgagacag atgaaaccta tgatgtttac
      661 tgctatgcag agcaaatgca aggcaaagtc ttctacgcca cctcccccga gaagttcacc
      721 ttcaatcact agtgaattcg cggccgcctg caggtcgacc atatgggaga gctcccaacg
      781 cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg
      841 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      901 gg
//
