LOCUS       sequence-430457-       14669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a gDNA under 
            regulation of CaMV 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14669)
  AUTHORS   Dombey R, Buendia-Avila D, Barragan-Borrero V, Diezma-Navas L, 
            Ponce-Mane A, Vargas-Guerrero JM, Elias R, Mari-Ordonez A
  TITLE     Atypical epigenetic and small RNA control of degenerated transposons
            and their fragments in clonally reproducing Spirodela polyrhiza
  JOURNAL   bioRxiv 2024.04.03.587901; doi: 
            https://doi.org/10.1101/2024.04.03.587901
REFERENCE   2  (bases 1 to 14669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.04.03.587901; doi: https://doi.org/10.1101/2024.04.03.587901"
COMMENT     Sequence Label: p35S-FHA-SpAGO4a_gDNA
FEATURES             Location/Qualifiers
     source          1..14669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    154..178
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    246..266
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        269..287
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     269..286
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        947..1291
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1235..1255
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1392..1416
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1422..1445
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1461..1487
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(6713..6737)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6750..6759
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        7124..7303
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7345..7896
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      7916..8168
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8261..8285
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8806..9597
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9843..10431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10332..10351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10585..10602
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10617..10757
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10683..10702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10843..10865
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11101..11295
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11361..12434)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12863..13492)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 caaagtactt taaagtactt taaagtactt taaagtactt tgatcccgag gggaaccctg
       61 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
      121 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
      181 cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctaagctt
      241 ggctccaact ttgtatagaa aagttgtcat ttaggtgaca ctatagaata ctcaagctat
      301 gcatccaacg cgttgggagc tctcccatat ggtcgactag agccaagctg atctcctttg
      361 ccccggagat caccatggac gactttctct atctctacga tctaggaaga aagttcgacg
      421 gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc ttcaatttca
      481 gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctc atcaagacga
      541 tctacccgag taataatctc caggagatca aataccttcc caagaaggtt aaagatgcag
      601 tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt ctcaagatca
      661 gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg caagtaatag
      721 agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag tcaaaaattc
      781 agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata cagagtcttt
      841 tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac actctcgtct
      901 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
      961 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
     1021 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
     1081 ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     1141 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata
     1201 tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta
     1261 tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa ttaccacaac
     1321 aaaacaaaca acaaacaaca ttacaattta ctattctagt cgacctgcag gcggccgcac
     1381 tagtgatatc agcaagtttg tacaaaaaag caggcttcat ggattacaag gatgacgatg
     1441 acaagggagg tggcggatcc tacccatacg atgttccaga ttacgctgga ggcggtccca
     1501 tgaaaagacc aggaacggga aagaatggac ggaccctttc attgctcacc aatcatttca
     1561 gagttgatat gcgtaacgtt gatgatggac atttctacca ctacagtgta cgtttcctta
     1621 tgctcttact ctgacgtgcg aaacatatat tttttttccg tacatgattt attctgtgat
     1681 ggtgtctctg ctgaaaatag gtgttgctca agtatgatga tgatcgccct gtggagggga
     1741 agggaattgg acgaagggta attgataagt tgcacgagat atatgatgct gaacttggtg
     1801 gcaaggattt tgcctatgat ggtgagaaaa gcttgttcac cattggctct ctgccccagg
     1861 tgaacaataa ttttactgta gtcctggagg atgtttcatc aaataggtat ttcctagcat
     1921 actatgtgtt ttgtaatttc tctctattcg atgtcaagtt tatttttcat tttgatcctg
     1981 gaattcctat attaaactca gaactgggga agtttcgaac ggaagctctg gtggaaatga
     2041 tagcctggag gatagtgaaa gaaagaggag gcgacggccc tatcaaatga agacttttaa
     2101 ggttgagctg agcttctctg caaaggttgc gatccagtcc attgcaaatg ctcttcgagg
     2161 acacgaaaat gagaattctc aggaagcact cagggtgctt gatatcatac taaggcagca
     2221 tgccgcgaag cagtaagctg ccatgagttt tataattttt ttcccttggt cttcaatatt
     2281 ttccatattt cactgtcaat tctcctattt gtagggggtg cctgcttgtt cggcaatcat
     2341 tttttcataa tgatcctaag aattatgtcg aattgggagg aggagtttca ggatgcaggg
     2401 gttttcattc gagctttcgg acaacccagg gtggtctgtc cctgaacatg ggtatgcttg
     2461 tagaaacttc cgaattttcg gccacctgtg tcattactct gttccagtta cacttctttc
     2521 tctatctgtt tcctggatgc ctaaaatatt gctcgtttta tctcaatttc atttcaagat
     2581 atggttaatg cttgttctac agatgtctca actacaatgg ttgtgaagcc gggtccagtc
     2641 atagattttc tcatggcaaa ccaaaaggct cgtaacgctc gagaaattga ctggctgaag
     2701 gtgatctgga attttgttaa gttgtctctg ggtgtcattt tgaacttctc tctcaacctt
     2761 ttaaccgaat tttctttttt ttaataggcc aaacgccagc tgaaaaatct tagggtaaaa
     2821 attacttcca caaacttgga gtttaaagtt gcgggattga gcgatcagcc atgtaaccaa
     2881 ctcaagtaag ttacagtttt gtgaaagaca gattatttct tcagctaact gattgcaaca
     2941 cttttctaat ttttttgaaa tgggtaggtt ttcactgaaa cagaagaatc gtggaaatgg
     3001 gagtggtgat tgtgatgtcg aagccgttga tataacagtg tatgaatatt ttgtcaacca
     3061 ccgtaacata aagttgacaa agtctgctga tctgccttgt gttaatgttg ggaaatcaaa
     3121 acgtcccaca tattatccat tggaggtttg gagatgctct tgtcatttaa acttcataac
     3181 tgtacctttt tatgtgcctt ttggatgaat ttatcctact tgtccagcta tgttctcttg
     3241 tgtcgctgca acgttataca aaggcactgt cgacattcca acgatcctca cttgtggaga
     3301 aatctcggca gaatccccaa gagaggatga gagttttaaa agatgtaggt gcatgctgtt
     3361 catgaatgag atgcatgtga caggctagat aatgttctaa ccgtcattcg attctttttg
     3421 tcagtcattg aagatgagtc gctatgatgc tgataggatg ctgaatgcat gcggcatttc
     3481 tatttgtccg gaatttatgc aggttgaagg tcgagtatta caaacaccga aggtttttcc
     3541 taaactattt tttcatgtta tcaggtcttt ctattagcac taattaactg tgtaactcaa
     3601 attcttctgc ttgtactgta gcttagagta ggtaatggtg aagactttat accacgcaat
     3661 gggcggtgga atttcaacga caaggtatct atttaaatgc ttaatcattc tacgaatttt
     3721 tacctgatgg caacttctct ttcagacatt tgcagaaccc acagccatcg acaaatgggc
     3781 tgtcgtcaat ttttctgttc gctgtgatgt aggccatttt attcgggaaa tgaaaaaatg
     3841 tgggcagatg aaaggaatcg tatgttcctt tttcataata atttctcttt tttttatcac
     3901 tggctactac gatctatatc actgaaggga tgggaatgtt tgtaattttt ttttgtagaa
     3961 tattgcgcat ccatttgatg tgtttgagga ggatgcttca tttaggcgag ctcctccaaa
     4021 tgttagggtg gacgaaatgt ttgagaaaat gaggaaaaag cttcctggga ttcctcgctt
     4081 tcttctgtgt attcttccag tgaggaagaa ttctgatctc tatggtgttt agcagctcct
     4141 tttccatttt ttttttcgtt gtttcccttc tttttagttc ctcatgcatg catgtgatct
     4201 taccttttgt tgttcgtcag gtccatggaa gcgcaagaac cttaccgaat ttggtattgt
     4261 tacccagtgt atcggtcccc ccactaaaat cagtgatcag tattttacaa atcttcttat
     4321 gaagatcaac agcaaagtga gctcggagat tatctttagc atgtttttaa tgttcctctc
     4381 tagaacaaaa ctgtcttatc taatcactgc tggctgtttt tcttctcgta gttggggggc
     4441 atcaattctt tgttacaggt ggagcttccg cattcaagca ctcttgttcc caaggtttcg
     4501 accatcatcc ttgggatgga cgtttcacat ggtccacctg gccaatctga tatcccatca
     4561 gttgccgcag taagatatca gatggtctct tctaaatttt atcaattatg aggatgctag
     4621 ataaccaaca tggtacatga tcactggaaa caactattct tcttgaaata gaaacttcgg
     4681 aataaattgg ttccattcca tgcaaactct tcgttctcgt ccctctgaaa attgcccaat
     4741 atatggggcc cagaatgttt tgtatcgact gtctatttgt gtcgtttgca ttataacatg
     4801 tgtggaagta tgtatagtaa taatatgcca ctaacaagtt cctgacaaca tactttgttt
     4861 ctctctgtaa gttccattta atatctctgt gaagccggat ttgttggtat gtttcttccg
     4921 ttcttactct ctacctctct ttacctgcca taggttgtcg gttcgcggca gtggccttca
     4981 atatctcgtt acagagcgga agtgcgcact cagtccccca aggttgagat gatagattct
     5041 ttatttaagc ctgaaccaga taatcatgat tcgggcataa ttcggtatgc tgtttcttat
     5101 ttttcccatg tttatatcat tggtctatat ttgtcctgcc cgctgctttt gcgtattttt
     5161 tctgttcttc ataatttttt gtatctggat aatttcaggg aactcttggt ggacttttat
     5221 gccacttctc agcaaacgaa acctgatcgg gtgataattt ttcggtatat attcgcgcta
     5281 ctggtttcgt ccatgagatt ttttctacac ttttggagtt attttggttt atatgcacgc
     5341 gcactatctg ttctgtcgaa attaattcga ttactggggt ctgaggtact catatccttg
     5401 gggcatttct tgaaatattt gccaaatgta ggaataagtg acgatcgtct ggactctgac
     5461 taccgattaa tctccacaac ttcgcttgat tctcaaaagt tagttgccgc ttcatcttat
     5521 gaataggggg ggaccttggt attttttatt gttcctaggg atggcgtgag cgaatcgcag
     5581 ttcaaccagg tgctgaacat tgagctggat caaatcatcc aggtgcttct tctccatcct
     5641 gccacactct cgatcgtatt aatacgagca gataatcgta attctgagga gggggcttgg
     5701 atcgtgtctc ccttaattct ctcaggcctg caagctcctc gacgacacgt ggcatccgag
     5761 gttcactgtg attgtagccc agaagaacca tcacaccaag ttctttcagg ccaattccgc
     5821 gcataatgtt cctgccggtc ggcccctgaa ccagaccccg aggtccgttg actttcctta
     5881 aagagaaaac tgttcgttgt tttctgacgg tgttcttccc tgatgacagg tacggtggtc
     5941 gacaccagag tgtgccatcc acgaaacttt gacttttaca tgtgctcgca tgccgggatg
     6001 atcgtgagct gaccttcaga aagtctcatt tcctctaatg ggggagataa tcgccaccac
     6061 tgtgctaaca agagttccct ggatttctga aacagggtac gacccgcccg actcactatc
     6121 atgtgctgta tgatgagatt ggcttctcag cggacgaact ccaagaactc gttcattctc
     6181 tctcctatgt gtaaggaacc tgttcttccg tactcatccg cgtcgccaca agtgaactca
     6241 aaagatctgt tacctttctt tcaggtacca acggagcacg accgccgttt ccctgggtaa
     6301 gcaattctct ctctatctct ctctctctgt cgcaatattt atacctaaat atttgttttt
     6361 tttctcataa gatattcatt tgaacttatt caagtttagt tcttcatgca tcatatttat
     6421 ccgattatta tgtgaaaatg gtacaattac gaacagtgtt acaattcaga tgtgtggagg
     6481 ggtggggatt ctgacgtggc ggctctctct gttcatcacc ttttttccag ttgctccaat
     6541 atgctatgcc cacttggcgg cggcccaggt gggacaattc atcaagttcg aggagtcctc
     6601 ggagacctca tccggccgcg gcggcgtgac ctccgccggc agcgtccccg tggtggcgct
     6661 gccgaggctt cacccccagg tgagcagctc catgttcttc tgctgactag atacccagct
     6721 ttcttgtaca aagtggtgat atcccgcggc catggcggcc gggagcatgc gacgtcgatc
     6781 taactgacta gccgcggcca tgctagagtc cgcaaaaatc accagtctct ctctacaaat
     6841 ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata agggaattag
     6901 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     6961 atttgtaaaa tacttctatc aataaaattt ctaatcccta aaaccaaaat ccagtgacct
     7021 cccgggtacc gcgaattatc gatcatgagc ggagaattaa gggagtcacg ttatgacccc
     7081 cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag
     7141 gagccactga gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat
     7201 tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa
     7261 atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc
     7321 tcaactcgat cgaggggatc taccatgagc ccagaacgac gcccggccga catccgccgt
     7381 gccaccgagg cggacatgcc ggcggtctgc accatcgtca accactacat cgagacaagc
     7441 acggtcaact tccgtaccga gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt
     7501 ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc
     7561 tacgcgggtc cctggaaggc acgcaacgcc tacgactgga cggccgagtc gaccgtgtac
     7621 gtctcccccc gccaccagcg gacgggactg ggctccacgc tctacaccca cctgctgaag
     7681 tccctggagg cacagggctt caagagcgtg gtcgctgtca tcgggctgcc caacgacccg
     7741 agcgtgcgca tgcacgaggc gctcggatat gccccccgcg gcatgctgcg ggcggccggc
     7801 ttcaagcacg ggaactggca tgacgtgggt ttctggcagc tggacttcag cctgccggtg
     7861 ccgccccgtc cggtcctgcc cgtcaccgaa atctgatgac ccctagagtc aagcagatcg
     7921 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     7981 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     8041 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     8101 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     8161 actagatcga ccggcatgca agctgataat tcaattcggc gttaattcag tacattaaaa
     8221 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     8281 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     8341 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     8401 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     8461 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     8521 tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     8581 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     8641 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     8701 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     8761 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     8821 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     8881 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     8941 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     9001 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     9061 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     9121 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     9181 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     9241 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     9301 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     9361 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     9421 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     9481 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     9541 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     9601 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     9661 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     9721 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc
     9781 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     9841 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     9901 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     9961 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
    10021 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10081 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10141 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10201 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10261 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10321 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10381 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10441 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10501 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10561 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
    10621 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
    10681 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
    10741 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
    10801 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
    10861 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
    10921 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
    10981 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
    11041 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
    11101 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
    11161 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
    11221 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
    11281 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
    11341 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
    11401 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
    11461 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
    11521 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
    11581 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
    11641 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
    11701 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
    11761 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
    11821 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
    11881 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
    11941 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
    12001 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
    12061 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
    12121 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
    12181 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
    12241 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
    12301 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
    12361 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
    12421 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
    12481 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
    12541 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
    12601 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
    12661 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
    12721 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
    12781 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
    12841 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
    12901 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
    12961 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    13021 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    13081 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    13141 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    13201 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    13261 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    13321 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    13381 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    13441 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    13501 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    13561 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    13621 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    13681 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    13741 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    13801 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    13861 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    13921 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    13981 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    14041 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    14101 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    14161 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    14221 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    14281 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    14341 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    14401 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    14461 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    14521 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    14581 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    14641 actgcactat agcagcggag gggttggat
//
