LOCUS       sequence-430466-        1430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of CREB1 domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Martinez-Yamout MA, Nasir I, Shnitkind S, Ellis JP, Berlow RB, Kroon
            G, Deniz AA, Dyson HJ, Wright PE
  TITLE     Glutamine-rich regions of the disordered CREB transactivation domain
            mediate dynamic intra- and intermolecular interactions.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Nov 21;120(47):e2313835120. doi: 
            10.1073/pnas.2313835120. Epub 2023 Nov 14.
  PUBMED    37971402
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-11-21"; volume: "120(47):e2313835120. 
            doi"; pages: " 10.1073/pnas.2313835120. Epub 2023 Nov 14"
COMMENT     Sequence Label: H6Gb1-CREB(1-280) T2C, C90A, T198C
FEATURES             Location/Qualifiers
     source          1..1430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             24..46
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             59..76
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             92..253
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             278..298
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1379..1396
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cnnngtcatt ccctctagaa tattttgttt aactttaaga aggagatata ccatgggcca
       61 tcatcatcat catcacagca gcggcatgac ttacaaactg atccttaacg gtaaaacttt
      121 gaaaggcgaa accactaccg aagctgttga tgctgcgact gcagaaaaag ttttcaaaca
      181 gtacgctaac gacaacggtg ttgacggtga atggacttac gacgatgcga ctaagacctt
      241 caccgttact gaagaattca gcagcggcag cagcggcgaa aacctgtatt ttcagggctc
      301 ccatatgtgc atggactctg gagcagacaa ccagcagagt ggagatgctg ctgtaacaga
      361 agctgaaagt caacaaatga cagttcaagc ccagccacag attgccacat tagcccaggt
      421 atccatgcca gcagctcatg cgacgtcatc tgctcccact gtaaccttag tgcagctgcc
      481 caatgggcag acagtccagg tccatggggt catccaggcg gcccagccat cagttattca
      541 gtctccacaa gtccaaacag ttcagtcttc cgctaaggac ttaaaaagac ttttctccgg
      601 aactcagatt tcaactattg cagaaagtga agattcacag gagtctgtgg atagtgtaac
      661 tgattcccaa aaacgaaggg aaatcctttc aaggaggcct tcctacagga aaattttgaa
      721 tgacttatct tctgatgcac caggggtgcc aaggattgaa gaagaaaaat cagaagaaga
      781 gacttcagcc cctgccatca ccactgtaac agtgccaacc ccgatttacc aaactagcag
      841 tgggcagtat attgccatta cccagggagg agcaatacag ctggctaaca atggttgcga
      901 tggggtacag ggcctgcaga cattaaccat gaccaatgca gctgccactc agccgggtac
      961 taccattcta caatatgcac agaccactga tggacagcag attctagtgc ccagcaacca
     1021 agttgttgtt caagctgcct ctggtgatgt acaaacatac cagattcgca cagcacccac
     1081 tagcaccatt gcccctggag ttgttatggc gtcctcccca gcacttccta cacagcctgc
     1141 tgaataatag gcacgaaaga gagaggttcg tctaatgaag aacagggaag cagcaagaga
     1201 atctcgtaga aagaagaaag aatatgtgaa atctttagag aacagagtgg cagtgcttga
     1261 aaaccaaaac aaaacattga ttgaggagct aaaagcactt aaggaccttt actcccacaa
     1321 gtcagattaa taggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca
     1381 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaagaagc
//
