LOCUS       sequence-43048-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Park HS, Hohn MJ, Umehara T, Guo LT, Osborne EM, Benner J, Noren CJ,
            Rinehart J, Soll D
  TITLE     Expanding the genetic code of Escherichia coli with phosphoserine.
  JOURNAL   Science. 2011 Aug 26;333(6046):1151-4.
  PUBMED    21868676
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-08-26"; volume: "333(6046)"; pages: "1151-4"
COMMENT     Sequence Label: pMYO127TAG-SepT
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             549..566
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      597..683
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atattctcaa cataaaaaac tttgtgtaat acttgtaacg ctatatggag attaactcaa
       61 tctagagggt attacatatg gttctgtctg aaggtgaatg gcagctggtt ctgcacgttt
      121 gggctaaagt tgaagctgac gttgctggtc acggtcagga catcctgatc cgtctgttca
      181 aatctcaccc ggaaaccctg gaaaaattcg accgtttcaa acacctgaaa accgaagctg
      241 aaatgaaggc ttctgaagac ctgaaaaaac acggtgttac cgttctgacc gctctgggtg
      301 ctatcctgaa gaaaaagggt caccacgaag ctgaactgaa accgctggct cagtctcacg
      361 ctaccaaaca caaaatcccg atcaaatacc tggagttcat ctctgaagct atcatccacg
      421 ttctgcactc tcgtcatccg ggtaacttcg gtgcttaggc tcagggtgct atgaacaaag
      481 ctctggaact gttccgtaaa gacatcgctg ctaaatacaa agaactgggt taccagggtg
      541 gttctggtca tcaccatcac catcactaag gcgcgccgta gggaactgcc aggcatcaaa
      601 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      661 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
      721 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
      781 ccatcctgac
//
