LOCUS       sequence-43051-e        5516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+NmCitrine-ABCB1a
FEATURES             Location/Qualifiers
     source          1..5516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..724)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4773..4907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4797..4816)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4851..4870
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5020..5040)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5020..5038)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5059..5075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5059..5075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5072..5094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5083..5099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5107..5113,5114..5131,5132..5137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5152..5173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5290..5307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcgattgatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      481 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      721 caaggaattc aaggcctctc gagccagtat ggtagagggt gacccacctg ctgtgggttt
      781 ctccaatggc aatggaacag tgcagatgaa tggaaatgga gctagctttg atggaccagc
      841 cgtgatcgat gtgtcgccta tacatgagga cgctccttct gctaagcatg cgagagactc
      901 tcctccccct gcctacagtg atgtcagtgg aacgaaggaa cagggaaatg gaggatctgt
      961 ggaaggagag ggtggagaga aggaagacaa gaaagagaca aaagtagtgg gcacgtttga
     1021 agtgtttcgc tttgcaactg gtctagacgt cgtgttcatt ctgctagctc ttgtgatctc
     1081 tctctgccat ggtgtggcct tgcctgccgt tcttcttcta tttggagaag tcacagattc
     1141 tttcatcacc acagcctccg tgaacgtgac agacaatttg gcagcctttg aggaatcggt
     1201 ggacagtatt ataactttct ccatctacta ttcctacctg ggttgtggag tactcgccct
     1261 ggcttacttt caggtggtgc tgtgggacgt ggcagccgag agacagattc acaaagtgag
     1321 gcttcggttt ttccatgcca tcctccgtca agagattgcc tggtttgatg tccataaagg
     1381 cggcgaactc aacacaaggc ttgcagatga cattgacaag atccgcaatg gcattggaga
     1441 caagcttggc atcatgctgc agtacacggc cacgttcgtt gctggcatca ccatcggctt
     1501 cgtgaagagc tggaagctca cccttgtcat cctggcagtc tctctgatac taattgtacc
     1561 cctggtggga tctacatccg tgattatcca gcggatgacc aagcaagccc tggatgccta
     1621 tgctaaggca ggggcgatcg cgggtgaggt cttctcaggc ataaggactg tggtcgcatt
     1681 caacggggag gagaaggaga tggtcaggta ctccagcaac ttggaccaag ctaaatcaaa
     1741 gacggtcaaa aaggattttg ccacactact tgcccagggc ttccttttct tctccatgtt
     1801 ctccagctat gccattgcct tctggtatgg cacagttctg tatttagata atgaaattac
     1861 acctggagat atcctaacga cattccttgc tgtgctcttt ggtgcgtttg ccattggcca
     1921 agcgggtccg aactacagtg acttcacaac agctcgggcg gctgcatcta gcatctggga
     1981 agtcatagac cagatcccta ccatcgactg cttctcgacc gacggaaaga aggagaagat
     2041 taccggacag gtgacctttg aaggggtcca tttctcgtac ccgtccagag catcggtcaa
     2101 ggtgctcaat gggatcaacc tgaaggtgga cgttgggaag acggtggcca tggtcggcag
     2161 cagtggatgc gggaagagca cctgcatcca gctcattcag aggttctatg atgttgctga
     2221 aggcagcatc aaaattgacg gcattgacat ccgtgacctc aacgtgagct ggcttcgaga
     2281 tcacatcggt gtcgtgagcc aggaacccat cctgtttgcc accacgatcg aggagaacat
     2341 ccgctatgga cgtcttgacg tcacccaggc agagattgag aaagccgccg aggaagccaa
     2401 cgcccatgac ttcatctcca aacttccaga gggttactcc acattggttg gagagcgagg
     2461 agctcagctg tctggtggtc agaagcagag gatagccatt gccagggcac tggtcaggaa
     2521 ccctacaatc ctactcctgg acgaagccac atcggctcta gacacggaga gtgaggctac
     2581 cgttcaactc gccctcgaaa aggcccagca cggtcgaacc accctcgtca tcgctcaccg
     2641 tctctcaacc atcttcaact ctgacctcat ctgcgctttc aaggagggcg tcatctccga
     2701 gcagggcacc catgaagagc tgatgaagaa cgagggcggt gtttatcaca cgctcgtcat
     2761 gaagcagggc atgaagaaag aggaggagga gaagaaagag aacgaggttc cgctcgatga
     2821 tgatgatgac gaagaggatg atagccaagg tgagaaggtc tacagagcag gctctggaaa
     2881 gaagaagctg acacgagtgc tatcacggac acagtcacag atgtctggag atgaagaaaa
     2941 acaggatgaa gacgagtacg agaaggagct tgagaagcat ttcagcatga tgcgagtctg
     3001 gaagctgaac accccagagt gtggtttcat cctcctggga tgcatcgggg ccgcaatcaa
     3061 tggggccgtc cagcctggat tcgccgtcgt cttcagtaaa attcttgggg cttacagcat
     3121 tacagataga gctgccctct ttgatgaagt gactatatac tgtgttctgt ttgctgccct
     3181 tggtctactc tccttactcg ccagtatcat acagggtgtt ggatttggca agtcgggagg
     3241 tgagctgaca ctgaggcttc gtaacatgat gtttagagct atcctcagac agaacatttc
     3301 tttctttgat gacaagagga atggaaccgg tgccttgaca actaaattgg cgacggatgt
     3361 ctctcttata caaggggtga ctggagtaag acttggtatg atctttgaag tgctcttcaa
     3421 catcggtgtt ggtatcgtga tctcgttcgt ctacagctgg cagatcgctt gtctccttct
     3481 ctttgccttc ctccccatcc tgtccttggc cggtatgatt ggttggaaga tcctccaggg
     3541 taactccatc ggaacagccg gtagccaggc tgaggttggg aagcttgtct ccgagtgtat
     3601 tgagaacata cgtaccgttc agtccctcaa ccgaggacag accttccatc tgaagtactg
     3661 tgaactccag aaccctccgt acaaacaagg gatcaaggga gctttcgccg ccggtcttgc
     3721 gttcggcttc tctcaagcta ccatcttctt tgcatactca gctactttcc ggcttggtgc
     3781 ccaccttgtg ggtactggag acctcacctt ccctgatgtt ttcctgtctt tctctgcctt
     3841 gatgtttggt gcctttggtc tcggtcgcgc tgcaggatct gtgccagact tctccaaggc
     3901 taaggtggca acaggagagc tcttctattt ggtcgaccgt agtccagaca ttgacacttt
     3961 cagcgatgac ggggagaaac cggcgagcta cggtggatcg gtgagcctca acaacgttcg
     4021 gttccgctac ccgacccgtc ctgatgtccc tgtcctgagg gggctgtccg tctcggtgga
     4081 ccctggcgag accctggctt tggtcggcag cagcggttgc ggcaagagca ccaccatcca
     4141 gctgatggag cgcttctatg accctcactc agggactgtg atgtttgact cccacgatgc
     4201 ctcgctgctc aacactcgct ggcagcgcgc ccaggtgggc ctcgtatccc aagaaccctg
     4261 cctcttcgac atgtccatcg cagagaacat caagtacggc gacaactctc gggaagtctc
     4321 catcgaagac tgcatcgaag cggccaaaaa gtccaacatc catgactttg tggactctct
     4381 tcccatgaaa tacgacacca acgttggatc caagggcacc cagctgtccg gcggtcagaa
     4441 gcagaggata gccattgcca gggcactggt caggaacccc aaggtcctgc tcctggatga
     4501 agccacatcg gccttggaca ctgaaagcga aagggttgta caagatgccc tggatgaagc
     4561 caagaagggc cggacctgca tcaccatcgc ccatcgcctc tcaaccatcc acaatgcaga
     4621 gaagatcgct gtcatccgag aaggaaagct cgccgagttt ggcaagcatg aagagctgat
     4681 ggctatgaag cagcaatatt attccctcta cactgcccag tctatgcagc attaactcga
     4741 gcctctagaa ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg
     4801 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4861 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     4921 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcgcggccg
     4981 cggcgccaat gcattgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
     5041 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5101 tccacacaac atacgagccg ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5161 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     5221 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     5281 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     5341 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     5401 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     5461 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcaga
//
