LOCUS       sequence-430519-        5463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase with mouse liver DNase hypersensitive site # 
            29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Chang TY, Waxman DJ
  TITLE     HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver 
            in vivo.
  JOURNAL   BMC Genomics. 2024 Dec 24;25(1):1240. doi: 
            10.1186/s12864-024-11162-9.
  PUBMED    39716078
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2024-12-24"; volume: "25(1):1240. doi"; pages: " 
            10.1186/s12864-024-11162-9"
COMMENT     Sequence Label: pTYC2_pPromALB_enh29774_FireflyLuc
FEATURES             Location/Qualifiers
     source          1..5463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1315..1324
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1321..2973
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3017..3138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3028..3047)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3082..3101
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3386..3403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3557..4145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3637..3656)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4345..5136,5137..5205))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5310..5358
                     /note="synthetic polyadenylation signal"
     misc_feature    5372..5463
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5412..5431
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct tctacaggga gataactatg ttttacagct attagatttt
       61 agggttatgt gagtcaggta ccatatagct gcttctcttt gccctcgata aagaattcac
      121 ctgtattcac tcaggcaata agtattatat agtaaacttc tgacacagat tttaatccac
      181 aggtagtgta attattaact aaactcattt ttatttgaaa gtcacagcct gagtcatgaa
      241 atgttgactc tcagggcata gttgatcctg gtgaacccag ttgaactgaa gcctgaagag
      301 aagagtgtaa ggtacagggg acttgggttc cagcttaact gtactaccct cagtctgtgt
      361 gactctctaa ggcccagctt cctcagctct gaaaaagaaa gggttttatc atgatgggca
      421 aagctaattg agggactcca tagggataca tttggtgtga aacctctgga aatgaaacta
      481 tcaaaattct gtcaaagatg ggtagaacat tgcctaactc ttcctccttg tctctgcaga
      541 ggtgacattt gctcagtttt gttttgtttt tgtttttgtt ctcctgagta catcagagtc
      601 aaataaggac ttctgtgttg tgtgaagctg atgtcctagg aacaaaatca gggagctgta
      661 catttggagt aattaaagat ggggggttga ttttgtttgc cctctggata gcttcagtgc
      721 aaggcatagg tcaccgcaaa ggccaacaga tcttatctca aagctaagtt tttccatgta
      781 cgaggtacaa tatataacat gtgtggtatg ctcccagatt tcctttaccc ataaaaaatt
      841 agaaacaaaa cttcctgctt taagtgtttt ttttttaaag agggaattta acagtaaaat
      901 atacatattt acttagttaa ttacttggcc tcgaggatat caagatctgg cctcggcggc
      961 caagctttgc tcaaatggga gacaaagaga ttaagctctt atgtaaaatt tgctgtttta
     1021 cataacttta atgaatggac aaagtcttgt gcatgggggt gggggtgggg ttagagggga
     1081 acagctccag atggcaaaca tacgcaaggg atttagtcaa acaacttttt ggcaaagatg
     1141 gtatgatttt gtaatggggt aggaaccaat gaaatgcgag gtaagtatgg ttaataatct
     1201 acagttattg gttaaagaag tatattagag cgagtctttc tgcacacaga tcaccttcct
     1261 atcaacccca ctagcctctg gcaaaaagct tggcaatccg gtactgttgg taaagccacc
     1321 atggaagatg ccaaaaacat taagaagggc ccagcgccat tctacccact cgaagacggg
     1381 accgccggcg agcagctgca caaagccatg aagcgctacg ccctggtgcc cggcaccatc
     1441 gcctttaccg acgcacatat cgaggtggac attacctacg ccgagtactt cgagatgagc
     1501 gttcggctgg cagaagctat gaagcgctat gggctgaata caaaccatcg gatcgtggtg
     1561 tgcagcgaga atagcttgca gttcttcatg cccgtgttgg gtgccctgtt catcggtgtg
     1621 gctgtggccc cagctaacga catctacaac gagcgcgagc tgctgaacag catgggcatc
     1681 agccagccca ccgtcgtatt cgtgagcaag aaagggctgc aaaagatcct caacgtgcaa
     1741 aagaagctac cgatcataca aaagatcatc atcatggata gcaagaccga ctaccagggc
     1801 ttccaaagca tgtacacctt cgtgacttcc catttgccac ccggcttcaa cgagtacgac
     1861 ttcgtgcccg agagcttcga ccgggacaaa accatcgccc tgatcatgaa cagtagtggc
     1921 agtaccggat tgcccaaggg cgtagcccta ccgcaccgca ccgcttgtgt ccgattcagt
     1981 catgcccgcg accccatctt cggcaaccag atcatccccg acaccgctat cctcagcgtg
     2041 gtgccatttc accacggctt cggcatgttc accacgctgg gctacttgat ctgcggcttt
     2101 cgggtcgtgc tcatgtaccg cttcgaggag gagctattct tgcgcagctt gcaagactat
     2161 aagattcaat ctgccctgct ggtgcccaca ctatttagct tcttcgctaa gagcactctc
     2221 atcgacaagt acgacctaag caacttgcac gagatcgcca gcggcggggc gccgctcagc
     2281 aaggaggtag gtgaggccgt ggccaaacgc ttccacctac caggcatccg ccagggctac
     2341 ggcctgacag aaacaaccag cgccattctg atcacccccg aaggggacga caagcctggc
     2401 gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg tggacttgga caccggtaag
     2461 acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg gccccatgat catgagcggc
     2521 tacgttaaca accccgaggc tacaaacgct ctcatcgaca aggacggctg gctgcacagc
     2581 ggcgacatcg cctactggga cgaggacgag cacttcttca tcgtggaccg gctgaagagc
     2641 ctgatcaaat acaagggcta ccaggtagcc ccagccgaac tggagagcat cctgctgcaa
     2701 caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg acgacgatgc cggcgagctg
     2761 cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga ccgagaagga gatcgtggac
     2821 tatgtggcca gccaggttac aaccgccaag aagctgcgcg gtggtgttgt gttcgtggac
     2881 gaggtgccta aaggactgac cggcaagttg gacgcccgca agatccgcga gattctcatt
     2941 aaggccaaga agggcggcaa gatcgccgtg taataattct agagtcgggg cggccggccg
     3001 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3061 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3121 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     3181 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag
     3241 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     3301 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     3361 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     3421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     3481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     3541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     3601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     3661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     3721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     3781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     3841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     3901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     3961 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4141 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     4201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     4261 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc
     4321 gcaaatgcta aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc
     4381 gatctgccta tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat
     4441 tcgtgagggc ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc
     4501 ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc
     4561 tgctactttg tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag
     4621 ttcgccagtg agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg
     4681 ctcgtcgttc ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg
     4741 atcacccata ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag
     4801 taagttggcc gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt
     4861 catgccatcc gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga
     4921 gtagtgtata cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc
     4981 acatagcagt actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc
     5041 aaggatcttg ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc
     5101 ttcagcatct tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc
     5161 cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca
     5221 atattattga agcatttatc agggttacta gtacgtctct caaggataag taagtaatat
     5281 taaggtacgg gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt
     5341 gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga
     5401 aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc
     5461 tct
//
