LOCUS       sequence-430537-        7183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NBU2 backbone with LacI(HQN) and P(ttg)_nanoluc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Huang BD, Groseclose TM, Wilson CJ
  TITLE     Transcriptional programming in a Bacteroides consortium.
  JOURNAL   Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8.
  PUBMED    35794179
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-6"; volume: "13(1):3901. doi"; pages: " 
            10.1038/s41467-022-31614-8"
COMMENT     Sequence Label: pBH104
FEATURES             Location/Qualifiers
     source          1..7183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1257..2339
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSHQTVSNVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(1276..1295)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(5627..5695,5696..6487)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5845..5864)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            6618..6727
                     /note="incP origin of transfer"
     rep_origin      join(6898..7183,1..103)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
       61 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac
      121 cgcggtggcc ctgtacaaag aaaattcgac aaactgttat ttttctatct atttatttga
      181 attttgagcg ctcaaaattt acctttgtcg gcaattttga gcgctcaaaa ttaaataaag
      241 atattctcgt caaacaaata taaataatat aaacatggtt tttactctgg aagattttgt
      301 tggcgattgg cgtcagaccg cgggttataa tttggatcaa gtcctggaac agggtggcgt
      361 aagctctctg ttccagaacc tgggtgtgag cgtgacgccg attcagcgca tcgttctgtc
      421 cggcgagaac ggtctgaaaa ttgatattca tgtgatcatc ccgtacgaag gcctgagcgg
      481 tgaccaaatg ggtcaaatcg agaaaatctt taaagtcgtc tacccagttg acgatcacca
      541 cttcaaggtt atcttgcatt acggtacgct ggtgattgat ggtgtgaccc cgaatatgat
      601 tgactatttc ggccgtccgt atgaaggcat tgccgttttt gacggtaaaa agatcaccgt
      661 caccggtacc ctgtggaatg gcaataagat tattgacgag cgtctgatta acccggacgg
      721 cagcctgctg ttccgcgtga ccatcaacgg tgtcacgggt tggcgtctgt gcgagcgcat
      781 cctggcataa tgaactgcac ttgctttgat aattaatgat aaacaatcta aaagcactct
      841 aatcgttatc ggagtgcttt tagattacta atcaaattgc ttctactaat tgcctatctt
      901 ccagtgatgg aacagcattt gaacgcattg gctgcaacaa tcagccttga tctggaagaa
      961 gcaatgaaag ctgctgttaa gtctccgaat caggtattgt tcctgacagg tgtattccca
     1021 tccggtaaac gcggatactt tgcagttgat ctgactcagg aataaattat aaattaaggt
     1081 aagaagattg taggataagc taatgaaata gaaaaaggat gccgtcacac aacttgtcgg
     1141 cattcttttt tgttttatta gttgaaaata tagtgaaaaa gttgcctaaa tatgtatgtt
     1201 aacaaattat ttgtcgtaac tttgcactcc aaatctgttt ttaacatatg gcactagtga
     1261 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctctcatcag accgtttcca
     1321 atgttgttaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
     1381 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
     1441 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
     1501 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
     1561 gcggcgtcga agcctgtaaa acggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
     1621 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
     1681 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
     1741 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
     1801 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
     1861 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
     1921 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
     1981 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
     2041 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
     2101 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     2161 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtat
     2221 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
     2281 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     2341 ctttcctcgg taccaaattc cagaaaagag gcctcccgaa aggggggcct tttttcgttt
     2401 tggtcctact tgtgcctgtt ctatttccta tgcggttcct ggccttttgc tggccttttg
     2461 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2521 agtgagctga tacccgatac ttgtgcctgt tctatttccg aaccgaccgc ttgtatgaat
     2581 gcatcaaaat tcgttttctc tacgttggat tccttgttgc tcatattgtg atgataattt
     2641 ctacaaatat agtcattggt aactatctat gaaactgttt gatactttta tagttgatta
     2701 aacttgttca tggcatttgc cttaatatca tccgctatgt caatgtaggg tttcatagct
     2761 ttgtagtcgc tgtgtcccgt ccatttcatg accacctgtg ccgggattcc gagagccagc
     2821 gcattgcaga tgaatgtcct ttttcctgca tgggtactga gcaaagcgta tttgggtgtg
     2881 acttcatcaa tacgttcatt tcccttgtag taggtttccc gtacaggctc gttgatttct
     2941 gccagttcac ccagctcttt caggtaatcg ttcatcttct ggttgctgat gacgggcaga
     3001 gccatgtaat tctcgaaatg gatgtccttg tatttgtcca gtatggcttt gctgtatttg
     3061 ttcagttcaa tcgtcaggct gtcggcagtc ttgactgtgg ttatttcgat gtggtcggac
     3121 ttcacatcgc ttcttttcag attgcgaaca tccgaatacc gcaaactcgt aaagcagcag
     3181 aacaggaaaa catcacgcac acgttccagg tattgcttat ccttgggtat ctggtagtct
     3241 ttcagcttgt tcagttcatc ccaagtcagg aagattactt ttttcgaggt ggttttcagt
     3301 ttcggtttga acgtatcgta tgcaatgttc tgatgatgtc ctttcttgaa gctccagcgc
     3361 aggaaccatt tgaggaatcc catttgcttg ccgatggtgc tgtttctcat atccttggtg
     3421 tcacgcagga agttgacgta ttcgttcaat ccaaactcgt tgaaatagtt gaacgttgca
     3481 tcctccttga actctttgag gtggttcctc actgctgcaa atttttcata ggtggatgcc
     3541 gtccagttat tctggttacc gcactctttt acaaactcat cgaacacctc ccaaaagctg
     3601 acaggggctt cttccggctg ttcttcactg gtgtctttca ttctcatgtt gaaagcttcc
     3661 ttcaactgtt gggtcgttgg catgacctcc tgcacctcaa attccttgaa aatattctgg
     3721 atttcggcat agtatttcag caagtccgta ttgatttcgg ctgcactttg ctttagcttg
     3781 ttggtacatc cgttctttac ccgctgctta tctacatccc atttggctac gtcaatccgg
     3841 tagcccgttg taaactcgat acgttggctg gcaaagatga cacgcatacg gatgggtacg
     3901 ttctctacga ttggcacacc gttctttttc cggctctcca atgcaaaaat gatgttgcgc
     3961 ttgatattca taattgggtg cgtttgaaat tctacaccca aatatacacc caattattga
     4021 gatagcaaaa gacatttaga aacatttact tttactctat attgtaattt acacttgatt
     4081 atcagtcgtt tgcagtttta tgatattctg tgaaagtata agttcgagag cctgtctctc
     4141 cgcaaaaaac gctgaaaatc agcagattgc aaaacaaaca ccctgtttta cacccaagaa
     4201 tgtaaagtcg ggtgtttttg ttttatttaa gataatacaa ccactacata ataaaagagt
     4261 agcgatatta aaagaatccg atgagaaaag actaatattt atctatccat tcagtttgat
     4321 ttctcaggac tttacatcgt cctgaaagta tttgttggta ccgaggacgc gtaaacattt
     4381 acagttgcat gtggcctatt gtttttagcc gttaaatatt ttataactat taaatagcga
     4441 tacaaattgt tcgaaactaa tattgtttat atcatatatt ctcgcatgtt ttaaagcttt
     4501 attaaattga ttttttgtaa acagtttttc gtactctttg ttaacccatt tcattacaaa
     4561 agtttcatat ttttttctct ctttaaatgc catttttgct ggctttcttt ttaatacaat
     4621 taatgtgcta tccactttag gttttggatg gaaataatac ctaggaattt ttgctaatat
     4681 agaaatatct acctctgcca ttaacagcaa tgctagtgat ctgtttgtat ctaataacat
     4741 tttagcaaaa ccatattcca ctattaaata acttattgtg gctgaacttt caaaaacaat
     4801 ttttcgaatt atatttgtgc ttatgttgta aggtatgctg ccaaatattt tatatggatt
     4861 gtggctagga aatgtaaatt tcagtatatc atcatttact atttgatagt taggataatt
     4921 taagagctta ttacgagtta cctcacataa tttagaatca atttctatcg ccgttacaaa
     4981 attacatctc tttaccaatc cagcagtaaa atgacctttc cctgcaccta tttcaaagat
     5041 gttatctttt tcatctaaac ttatgcaatt cattattttt tctatgtgat attttgaagt
     5101 aataaaattt tgactatctt ttatatttac tttgttcatt ataacctctc cttaatttat
     5161 tgcatctctt ttcgaatatt tatgtttttt gagaaaagaa cgtactcatg gttcatcccg
     5221 atatgcgtat cggtctgtat atcagcaact ttctatgtgt ttcaactaca atagtcatct
     5281 attctcatct ttctgagtcc accccctgca aagcccctct ttacgacata aaaattcggt
     5341 cggaaaaggt atgcaaaaga tgtttctctc tttaagagaa actcttcggg atgcaaaaat
     5401 atgaaaataa ctccaattca ccaaattata tagcgacttt tttacaaaat gctaaaattt
     5461 gttgatttcc gtcaagcaat tgttgagcaa aaatgtcttt tacgataaaa tgatacctca
     5521 atatcaactg tttagcaaaa cgatatttct cttaaagaga gaaacacctt tttgttcacc
     5581 aatccccgac ttttaatccc gcggccatga ttgaaaaagg aagagtatga gtattcaaca
     5641 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5701 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5761 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5821 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5881 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5941 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6001 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6061 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6121 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6181 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6241 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6301 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6361 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6421 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6481 ttggtaactg tcagaccaag tttactcata acgcgtcaat tcgaggggga tcaattccgt
     6541 gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc
     6601 tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag
     6661 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
     6721 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
     6781 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
     6841 gcagggttat gcagcggaaa acggaattga tccggccacg atgcgtccgg cgtagaggat
     6901 ctgaagatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact
     6961 aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa
     7021 cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat
     7081 gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt
     7141 tataagaaaa aaaagaatat ataaggcttt taaagctttt aag
//
