LOCUS       sequence-430539-        7045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NBU2 backbone with LacI(TAN) and P(tta)_nanoluc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Huang BD, Groseclose TM, Wilson CJ
  TITLE     Transcriptional programming in a Bacteroides consortium.
  JOURNAL   Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8.
  PUBMED    35794179
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-6"; volume: "13(1):3901. doi"; pages: " 
            10.1038/s41467-022-31614-8"
COMMENT     Sequence Label: pBH102
FEATURES             Location/Qualifiers
     source          1..7045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1250..2332
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSTATVSNVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(1269..1288)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             join(5489..5557,5558..6349)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5707..5726)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            6480..6589
                     /note="incP origin of transfer"
     rep_origin      join(6760..7045,1..103)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc ttagagcctc
       61 tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggaat tcccctccac
      121 cgcggtggta caaagaaaat tcgacaaact gttatttttc tatctattta tttgaatttt
      181 aagcgcttaa aatttacctt tgtcggcaat tttaagcgct taaaattaaa taaagatatt
      241 ctcgtcaaac aaatataaat aatataaaca tggtttttac tctggaagat tttgttggcg
      301 attggcgtca gaccgcgggt tataatttgg atcaagtcct ggaacagggt ggcgtaagct
      361 ctctgttcca gaacctgggt gtgagcgtga cgccgattca gcgcatcgtt ctgtccggcg
      421 agaacggtct gaaaattgat attcatgtga tcatcccgta cgaaggcctg agcggtgacc
      481 aaatgggtca aatcgagaaa atctttaaag tcgtctaccc agttgacgat caccacttca
      541 aggttatctt gcattacggt acgctggtga ttgatggtgt gaccccgaat atgattgact
      601 atttcggccg tccgtatgaa ggcattgccg tttttgacgg taaaaagatc accgtcaccg
      661 gtaccctgtg gaatggcaat aagattattg acgagcgtct gattaacccg gacggcagcc
      721 tgctgttccg cgtgaccatc aacggtgtca cgggttggcg tctgtgcgag cgcatcctgg
      781 cataatgaac tgcacttgct ttgataatta atgataaaca atctaaaagc actctaatcg
      841 ttatcggagt gcttttagat tactaatcaa attgcttcta ctaattgcct atcttccagt
      901 gatggaacag catttgtgca ttggctgcaa caatcagcct tgatctggaa gaagcaatga
      961 aagctgctgt taagtctccg aatcaggtat tgttcctgac aggtgtattc ccatccggta
     1021 aacgcggata ctttgcagtt gatctgactc aggaataaat tataaattaa ggtaagaaga
     1081 ttgtaggata agctaatgaa atagaaaaag gatgccgtca cacaacttgt cggcattctt
     1141 ttttgtttta ttagttgaaa atatagtgaa aaagttgcct aaatatgtat gttaacaaat
     1201 tatttgtcgt aactttgcac tccaaatctg tttttaacat atggcactag tgaaaccagt
     1261 aacgttatac gatgtcgcag agtatgccgg tgtctctacc gcgaccgttt ccaacgtggt
     1321 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga
     1381 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat
     1441 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa
     1501 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt
     1561 cgaagcctgt aaaacggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat
     1621 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc
     1681 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga
     1741 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct
     1801 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata
     1861 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc
     1921 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt
     1981 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt
     2041 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc
     2101 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt
     2161 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt
     2221 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2281 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagctttcct
     2341 cggtaccaaa ttccagaaaa gaggcctccc gaaagggggg ccttttttcg ttttggtcct
     2401 acttgtgcct gttctatttc cgaaccgacc gcttgtatga atgcatcaaa attcgttttc
     2461 tctacgttgg attccttgtt gctcatattg tgatgataat ttctacaaat atagtcattg
     2521 gtaactatct atgaaactgt ttgatacttt tatagttgat taaacttgtt catggcattt
     2581 gccttaatat catccgctat gtcaatgtag ggtttcatag ctttgtagtc gctgtgtccc
     2641 gtccatttca tgaccacctg tgccgggatt ccgagagcca gcgcattgca gatgaatgtc
     2701 ctttttcctg catgggtact gagcaaagcg tatttgggtg tgacttcatc aatacgttca
     2761 tttcccttgt agtaggtttc ccgtacaggc tcgttgattt ctgccagttc acccagctct
     2821 ttcaggtaat cgttcatctt ctggttgctg atgacgggca gagccatgta attctcgaaa
     2881 tggatgtcct tgtatttgtc cagtatggct ttgctgtatt tgttcagttc aatcgtcagg
     2941 ctgtcggcag tcttgactgt ggttatttcg atgtggtcgg acttcacatc gcttcttttc
     3001 agattgcgaa catccgaata ccgcaaactc gtaaagcagc agaacaggaa aacatcacgc
     3061 acacgttcca ggtattgctt atccttgggt atctggtagt ctttcagctt gttcagttca
     3121 tcccaagtca ggaagattac ttttttcgag gtggttttca gtttcggttt gaacgtatcg
     3181 tatgcaatgt tctgatgatg tcctttcttg aagctccagc gcaggaacca tttgaggaat
     3241 cccatttgct tgccgatggt gctgtttctc atatccttgg tgtcacgcag gaagttgacg
     3301 tattcgttca atccaaactc gttgaaatag ttgaacgttg catcctcctt gaactctttg
     3361 aggtggttcc tcactgctgc aaatttttca taggtggatg ccgtccagtt attctggtta
     3421 ccgcactctt ttacaaactc atcgaacacc tcccaaaagc tgacaggggc ttcttccggc
     3481 tgttcttcac tggtgtcttt cattctcatg ttgaaagctt ccttcaactg ttgggtcgtt
     3541 ggcatgacct cctgcacctc aaattccttg aaaatattct ggatttcggc atagtatttc
     3601 agcaagtccg tattgatttc ggctgcactt tgctttagct tgttggtaca tccgttcttt
     3661 acccgctgct tatctacatc ccatttggct acgtcaatcc ggtagcccgt tgtaaactcg
     3721 atacgttggc tggcaaagat gacacgcata cggatgggta cgttctctac gattggcaca
     3781 ccgttctttt tccggctctc caatgcaaaa atgatgttgc gcttgatatt cataattggg
     3841 tgcgtttgaa attctacacc caaatataca cccaattatt gagatagcaa aagacattta
     3901 gaaacattta cttttactct atattgtaat ttacacttga ttatcagtcg tttgcagttt
     3961 tatgatattc tgtgaaagta taagttcgag agcctgtctc tccgcaaaaa acgctgaaaa
     4021 tcagcagatt gcaaaacaaa caccctgttt tacacccaag aatgtaaagt cgggtgtttt
     4081 tgttttattt aagataatac aaccactaca taataaaaga gtagcgatat taaaagaatc
     4141 cgatgagaaa agactaatat ttatctatcc attcagtttg atttctcagg actttacatc
     4201 gtcctgaaag tatttgttgg taccgaggac gcgtaaacat ttacagttgc atgtggccta
     4261 ttgtttttag ccgttaaata ttttataact attaaatagc gatacaaatt gttcgaaact
     4321 aatattgttt atatcatata ttctcgcatg ttttaaagct ttattaaatt gattttttgt
     4381 aaacagtttt tcgtactctt tgttaaccca tttcattaca aaagtttcat atttttttct
     4441 ctctttaaat gccatttttg ctggctttct ttttaataca attaatgtgc tatccacttt
     4501 aggttttgga tggaaataat acctaggaat ttttgctaat atagaaatat ctacctctgc
     4561 cattaacagc aatgctagtg atctgtttgt atctaataac attttagcaa aaccatattc
     4621 cactattaaa taacttattg tggctgaact ttcaaaaaca atttttcgaa ttatatttgt
     4681 gcttatgttg taaggtatgc tgccaaatat tttatatgga ttgtggctag gaaatgtaaa
     4741 tttcagtata tcatcattta ctatttgata gttaggataa tttaagagct tattacgagt
     4801 tacctcacat aatttagaat caatttctat cgccgttaca aaattacatc tctttaccaa
     4861 tccagcagta aaatgacctt tccctgcacc tatttcaaag atgttatctt tttcatctaa
     4921 acttatgcaa ttcattattt tttctatgtg atattttgaa gtaataaaat tttgactatc
     4981 ttttatattt actttgttca ttataacctc tccttaattt attgcatctc ttttcgaata
     5041 tttatgtttt ttgagaaaag aacgtactca tggttcatcc cgatatgcgt atcggtctgt
     5101 atatcagcaa ctttctatgt gtttcaacta caatagtcat ctattctcat ctttctgagt
     5161 ccaccccctg caaagcccct ctttacgaca taaaaattcg gtcggaaaag gtatgcaaaa
     5221 gatgtttctc tctttaagag aaactcttcg ggatgcaaaa atatgaaaat aactccaatt
     5281 caccaaatta tatagcgact tttttacaaa atgctaaaat ttgttgattt ccgtcaagca
     5341 attgttgagc aaaaatgtct tttacgataa aatgatacct caatatcaac tgtttagcaa
     5401 aacgatattt ctcttaaaga gagaaacacc tttttgttca ccaatccccg acttttaatc
     5461 ccgcggccat gattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5521 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5581 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5641 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5701 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5761 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5821 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5881 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5941 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6001 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6061 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6121 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6181 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6241 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6301 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6361 agtttactca taacgcgtca attcgagggg gatcaattcc gtgataggtg ggctgccctt
     6421 cctggttggc ttggtttcat cagccatccg cttgccctca tctgttacgc cggcggtagc
     6481 cggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat aagggacagt
     6541 gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccg gctgacgccg
     6601 ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat atcgtgcgaa
     6661 aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt atgcagcgga
     6721 aaacggaatt gatccggcca cgatgcgtcc ggcgtagagg atctgaagat cagcagttca
     6781 acccgttgat agtacgtact aagctctcat gtttcacgta ctaagctctc atgtttaacg
     6841 tactaagctc tcatgtttaa cgaactaaac cctcatggct aacgtactaa gctctcatgg
     6901 ctaacgtact aagctctcat gtttcacgta ctaagctctc atgtttgaac aataaaatta
     6961 atataaatca gcaacttaaa tagcctctaa ggttttaagt tttataagaa aaaaaagaat
     7021 atataaggct tttaaagctt ttaag
//
