LOCUS       sequence-430544-        7165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NBU1 backbone with BsmBI restriction sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Huang BD, Groseclose TM, Wilson CJ
  TITLE     Transcriptional programming in a Bacteroides consortium.
  JOURNAL   Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8.
  PUBMED    35794179
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-07-6"; volume: "13(1):3901. doi"; pages: " 
            10.1038/s41467-022-31614-8"
COMMENT     Sequence Label: pBH002
FEATURES             Location/Qualifiers
     source          1..7165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(136..141,142..159,160..166)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    174..190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     179..201
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     198..214
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     198..214
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             210..533
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    227..283
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(284..301)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(284..300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(293..315)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(509..528)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             join(5581..5649,5650..6441)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5799..5818)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     oriT            6572..6681
                     /note="incP origin of transfer"
     rep_origin      join(6852..7165,1..75)
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttgagtg acacaggaac
       61 acttaacggc tgacatggga attcccctcc accgcggtgt attaatgcgg ctgccccttg
      121 agacggcatc ttccatctac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      181 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgcatgcct
      241 gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac
      301 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      361 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      421 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
      481 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca taggatctga
      541 tgcatcgtct caccgataat tgcctatctt ccagtgatgg aacagcattt gtgcattggc
      601 tgcaacaatc agccttactt gtgcctgttc tatttccgaa ccgaccgctt gtatgaatgc
      661 atcaaaattc gttttctcta cgttggattc cttgttgctc atattgtgat gataatttct
      721 acaaatatag tcattggtaa ctatctatga aactgtttga tacttttatc agtccagtag
      781 ttttacaagg tctttcttca tttcttcgtc aatatccctg tatcgtctga aagctttgct
      841 tccctccttg tgtcccgaca gtgcggaaac aaggttcggg tctttcactt ttttatagat
      901 attgccgata aacgtacgtc ttgccagatg gctgcttgcc acttcataaa taggtcgttt
      961 gatttcgttg tgcgtcaacg ggtctaagat tgttacgatg cggtcaactc cagctaattt
     1021 gaatatcttt tttatggcat cattgtactt ttgctcggat atgaacggca acagttttcc
     1081 ctcatattct ttgtagcgtt caaggatttc tttcgctttg tcgttaagtg gaacacgtac
     1141 cgtaaccgga ttcccctctt tggttttctt gggaatatat tctatggctt cattgaccac
     1201 atttagtttg gtcattcggt acaggtcgct caccctgcat cctatcagtg tctgaaatat
     1261 gaatatatcc ctctgtattg ccagttgtgg ggtggcagaa aggtctgcat taaaaatcct
     1321 gtccctttct tcgagtgtta tataataagg tgtaccatat gtacactcct ctatcggaaa
     1381 cttgtcgaaa ggtctgtttg tggtgcgttt gttatcgaag caccacagga agaatgtgcg
     1441 tattcttgaa aaacagtcta tcagcgtgtt tttgcttctg ggctgtggtg tcctcttttc
     1501 gggaatggct tcataaatgc tcgggtaaag ttcataatac tggtattcgt tctgaaagaa
     1561 atcccacata tcccgaagcg tgtcaggtgt taccaaatcc acatcaagga taaagccctt
     1621 ttgtcctctc tttgtagccc ttacatatag ttcataacgc agtaaggctc ttttgacaac
     1681 ccggaaattt ttctttcgta cttccgacaa agggtgcttg tttagaaatt catcgaatag
     1741 ttctccaatg gtaggcttga taaccacttc ctccggcaaa aaatattttt caggatggta
     1801 aaatttatca agtgttgttt ttagccattc tttgtctatt gcttcctttt cttgttgata
     1861 taccttctcg atataggttt tcaactggcg tatctcttca tttatatggg tacgcatttc
     1921 ttcattgcag acagctttcg tttttacaca ttcgtcttta tcatcccata gattgggatt
     1981 gatggctaac tgagtaggag caacggagtc taactgcctt ccgtttctaa aacggacata
     2041 gattgtagcc atggattctg tatcatatcg tttggctgct tttttaatga taaaggttac
     2101 tttcatagac tttcaggttg aattttactc tgctgcaaat ataaatattt tccccagcat
     2161 tttccccaca tctgctaaat attttgcaat tcgattaaac ttggattaaa atttaatagg
     2221 attataacat attgaaatac agtgtaattg tggcgttttt ccgcattttt ctttttaccc
     2281 acattttccc cactttaatt atttcaaatg cggggtctgg gtacaagaaa gaaagctaag
     2341 tatttgatag ttcaatactt agctttttct tttgcttgaa ttttccccac attttcccca
     2401 cacgtgcaaa aaatatagca gtaagtcatt atttcttttg gttgaacgta gagagtagcg
     2461 atattaaaag aatccgatga gaaaagacta atatttatct atccattcag tttgatttct
     2521 caggacttta catcgtcctg aaagtatttg ttggtaccga ggacgcgtaa acatttacag
     2581 ttgcatgtgg cctattgttt aggacgcgtt atctccttaa cgtacgtttt cgttccattg
     2641 gccctcaaac cccgttatat acattcatgt ccatttatgt aaaaaatcct gctgaccttg
     2701 tttatgtctt gtcagtcacc atttgcaaaa ccatatttga ccctcaaaga ggctgaattt
     2761 gataagcaac ttgctacata ctcataataa ggagctaaat agaacacgaa tgggaaatac
     2821 tcaaatgcca aactaaagaa gatattggcc aaaataaacg ctataccgag agagaaactt
     2881 gatttttcaa cttcctaaaa cagtgttgtt caaacatttc tacttatttg tacttaccag
     2941 ttgaacctac gtttccctaa taaaatgtct atggtaaaaa gttaaaaaat cctcctactt
     3001 ttgttagata tatttttttg tgtaattttg taatcgttat gcggcagtaa taatatacat
     3061 attaatacga gttaggaatc ctgtagttct catatgctac gaggaggtat taaaaggtgc
     3121 gtttcgacaa tgcatctatt gtagtatatt attgcttaat ccaaatgaat attataaatt
     3181 taggaattct tgctcacatt gatgcaggaa aaacttccgt aaccgagaat ctgctgtttg
     3241 ccagtggagc aacggaaaag tgcggctgtg tggataatgg tgacaccata acggactcta
     3301 tggatataga gaaacgtaga ggaattactg ttcgggcttc tacgacatct attatctgga
     3361 atggtgtgaa atgcaatatc attgacactc cgggacacat ggattttatt gcggaagtgg
     3421 agcggacatt caaaatgctt gatggagcag tcctcatctt atccgcaaag gaaggcatac
     3481 aagcgcagac aaagttgctg ttcaatactt tacagaagct gcaaatcccg acaattatat
     3541 ttatcaataa gattgaccga gccggtgtga atttggagcg tttgtatctg gatataaaag
     3601 caaatctgtc tcaagatgtc ctgtttatgc aaaatgttgt cgatggatcg gtttatccgg
     3661 tttgctccca aacatatata aaggaagaat acaaagaatt tgtatgcaac catgacgaca
     3721 atatattaga acgatatttg gcggatagcg aaatttcacc ggctgattat tggaatacga
     3781 taatcgctct tgtggcaaaa gccaaagtct atccggtgct acatggatca gcaatgttca
     3841 atatcggtat caatgagttg ttggacgcca tcacttcttt tatacttcct ccggcatcgg
     3901 tctcaaacag actttcatct tatctttata agatagagca tgaccccaaa ggacataaaa
     3961 gaagttttct aaaaataatt gacggaagtc tgagacttcg tgacgttgta agaatcaacg
     4021 attcggaaaa attcatcaag attaaaaatc taaaaactat caatcagggc agagagataa
     4081 atgttgatga agtgggcgcc aatgatatcg cgattgtaga ggatatggat gattttcgaa
     4141 tcggaaatta tttaggtgct gaaccttgtt tgattcaagg attatcgcat cagcatcccg
     4201 ctctcaaatc ctccgtccgg ccagacaggc ccgaagagag aagcaaggtg atatccgctc
     4261 tgaatacatt gtggattgaa gacccgtctt tgtccttttc cataaactca tatagtgatg
     4321 aattggaaat ctcgttatat ggtttaaccc aaaaggaaat catacagaca ttgctggaag
     4381 aacgattttc cgtaaaggtc cattttgatg agatcaagac tatatacaaa gaacgacctg
     4441 taaaaaaggt caataagatt attcagatcg aagtgccgcc caacccttat tgggccacaa
     4501 tagggctgac tcttgaaccc ttaccgttag ggacagggtt gcaaatcgaa agtgacatct
     4561 cctatggtta tctgaaccat tcttttcaaa atgccgtttt tgaagggatt cgtatgtctt
     4621 gccaatccgg gttacatgga tgggaagtga ctgatctgaa agtaactttt actcaagccg
     4681 agtattatag cccggtaagt acacctgctg atttcagaca gctgacccct tatgtcttca
     4741 ggctggcctt gcaacagtca ggtgtggaca ttctcgaacc gatgctctat tttgagttgc
     4801 agatacccca agcggcaagt tccaaagcta ttacagattt gcaaaaaatg atgtctgaga
     4861 ttgaagacat cagttgcaat aatgagtggt gtcatattaa agggaaagtt ccattaaata
     4921 caagtaaaga ctatgcatca gaagtaagtt catacactaa gggcttaggc atttttatgg
     4981 ttaagccatg cgggtatcaa ataacaaaag gcggttattc tgataatatc cgcatgaacg
     5041 aaaaagataa acttttattc atgttccaaa aatcaatgtc atcaaaataa tggtataacc
     5101 tctccttaat ttattgcatc tcttttcgaa tatttatgtt ttttgagaaa agaacgtact
     5161 catggttcat cccgatatgc gtatcggtct gtatatcagc aactttctat gtgtttcaac
     5221 tacaatagtc atctattctc atctttctga gtccaccccc tgcaaagccc ctctttacga
     5281 cataaaaatt cggtcggaaa aggtatgcaa aagatgtttc tctctttaag agaaactctt
     5341 cgggatgcaa aaatatgaaa ataactccaa ttcaccaaat tatatagcga cttttttaca
     5401 aaatgctaaa atttgttgat ttccgtcaag caattgttga gcaaaaatgt cttttacgat
     5461 aaaatgatac ctcaatatca actgtttagc aaaacgatat ttctcttaaa gagagaaaca
     5521 cctttttgtt caccaatccc cgacttttaa tcccgcggcc atgattgaaa aaggaagagt
     5581 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5641 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5701 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5761 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5821 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5881 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5941 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6001 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6061 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6121 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6181 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6241 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtca
     6301 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6361 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6421 tcactgatta agcattggta actgtcagac caagtttact cataacgcgt caattcgagg
     6481 gggatcaatt ccgtgatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc
     6541 cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg
     6601 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
     6661 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga
     6721 accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct
     6781 ataatgaccc cgaagcaggg ttatgcagcg gaaaacggaa ttgatccggc cacgatgcgt
     6841 ccggcgtaga ggatctgaag atcagcagtt caacctgttg atagtacgta ctaagctctc
     6901 atgtttcacg tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa
     6961 accctcatgg ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg
     7021 tactaagctc tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct
     7081 aaggttttaa gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt
     7141 taacggttgt ggacaacaag ccagg
//
